CN204832039U - Categorised detection device of alga - Google Patents
Categorised detection device of alga Download PDFInfo
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- CN204832039U CN204832039U CN201520546821.5U CN201520546821U CN204832039U CN 204832039 U CN204832039 U CN 204832039U CN 201520546821 U CN201520546821 U CN 201520546821U CN 204832039 U CN204832039 U CN 204832039U
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Abstract
The utility model discloses a categorised detection device of alga, it includes light source subassembly, sample cell and light signal processing subassembly, the light source subassembly includes the light source that a plurality of center wavelength are different to light source through these a plurality of differences sends different exciting lights extremely sample in the sample cell makes the sample is because of the quilt different exciting lights arouses and produces corresponding fluorescence, passes through light signal processing subassembly converts the multichannel signal of telecommunication, rethread computer basis into the multichannel signal of telecommunication carries out the categorised detection of alga. The utility model discloses a categorised detection device of alga can avoid the problem that the alga type is too single that detects simultaneously.
Description
Technical field
The utility model relates to technical field of biological, particularly a kind of alga classifying pick-up unit.
Background technology
In view of state, the aggravation of inside and outside water body eutrophication degree and the needs to the algae research that body eutrophication causes, how to realize type and the quantity of algae in the real-time analysis style of calligraphy, and Eutrophic Extent is judged, more and more cause the extensive attention of water quality monitoring department.
At present, the method for monitoring algae in water body both at home and abroad mainly adopts testing laboratory's microscope monitoring method and chlorophyll detection technique.Laboratory microscope monitoring method, by the method for morphological analysis and artificial counting, realizes the classification to algae in water body, and efficiency is low and difficulty is comparatively large, and very difficult realization labor further, has larger limitation.Although chlorophyll detection technique can judge the total body density of algae, be difficult to distinguish dissimilar algae and analyze.
Therefore, be necessary to propose the algae that a kind of alga classifying pick-up unit can detect number of different types simultaneously, to pass through growth course and the period of analyzing dissimilar algae, its growth tendency made a decision.
Utility model content
The purpose of this utility model is to provide a kind of structure simple alga classifying pick-up unit, for solving the too single problem of algae type that algae pick-up unit of the prior art detects simultaneously.
In order to solve the problems of the technologies described above, the utility model provides following technical scheme:
A kind of alga classifying pick-up unit, comprise light source assembly, sample cell and optical signal prosessing assembly, described light source assembly comprises the different light source of multiple centre wavelength, to be sent the sample in different exciting light to described sample cell by the plurality of different light source, make described sample because being produced corresponding fluorescence by described different excitation, be converted to multi-channel electric signal through described optical signal prosessing assembly, then carry out alga classifying detection by computing machine according to described multi-channel electric signal.
Preferably, described sample cell is T-shaped, and its small end is arranged with described light source assembly, and is connected with described optical signal prosessing assembly.
Preferably, seal is equipped with between the junction of described optical signal prosessing assembly and light source assembly and described sample cell.
Preferably, the quantity of described light source is three.
Preferably, the centre wavelength of described three light sources is respectively 590nm-610nm, 501nm-540nm and 455nm-490nm.
Preferably, modulated described three light sources by modulator-demodular unit, modulating frequency is respectively 700Hz, 900Hz and 1100Hz.
Preferably, described light source assembly also comprises a circular shell, and described multiple light source is evenly laid in described circular shell, and the material of described circular shell is quartz.
Preferably, described optical signal prosessing assembly comprises lens, filter plate and photomultiplier, and described fluorescence, after described lens light gathering, is incident to filtering parasitic light on described filter plate, re-shoots to described photomultiplier and carries out opto-electronic conversion.
Preferably, the selected wavelength of described filter plate is 680nm.
Preferably, described optical signal prosessing assembly also comprises a tubular shell, and described lens, filter plate and photomultiplier are uniform located therein, and the material of described tubular shell is metal.
Therefore compared with prior art, the utility model has following beneficial effect:
By arranging the different light source of multiple centre wavelength in light source assembly, the multiple light sources utilizing this centre wavelength different send different exciting lights to the sample in sample cell, make sample because being produced corresponding fluorescence by different excitation, and be converted to multi-channel electric signal through optical signal prosessing assembly, then carry out alga classifying detection by computing machine according to this multi-channel electric signal.That is, because the light source that multiple centre wavelength is different can inspire multiple exciting light to sample simultaneously, make the dissimilar algae comprised in sample can form different fluorescent effects, the fluorescence spectrum formed by the fluorescent effect that computing machine is different to this is carried out detecting and can detect dissimilar algae, thus avoids the too single problem of the algae type that simultaneously detects.
Accompanying drawing explanation
In order to be illustrated more clearly in each embodiment of the utility model or technical scheme of the prior art, be briefly described to the accompanying drawing used required in each embodiment of the utility model or description of the prior art below.Apparently, the accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structured flowchart of the alga classifying pick-up unit of an embodiment.
Fig. 2 is the structured flowchart of the alga classifying pick-up unit of another embodiment.
Fig. 3 is the partial cutaway schematic of the alga classifying pick-up unit of another embodiment.
Wherein, description of reference numerals is as follows:
100, alga classifying pick-up unit; 1, light source assembly; 11, light source; 2, optical signal prosessing assembly; 21, lens; 22, filter plate; 23, photomultiplier; 3, sample cell.
Embodiment
Technical scheme in the utility model is understood better in order to make those of ordinary skill in the art, and enable above-mentioned purpose of the present utility model, feature and advantage become apparent more, below in conjunction with accompanying drawing, the technical scheme in each embodiment of the present utility model is elaborated further.
Refer to Fig. 1, in one embodiment, a kind of alga classifying pick-up unit 100 comprises light source assembly 1, sample cell 3 and optical signal prosessing assembly 2.
Wherein, light source assembly 1 comprises the different light source of multiple centre wavelength, and light source can be the wide spectrum light source such as laser instrument or LED.
Sample in sample cell 3 is dissimilar algae, and it is light tight but can intake, and is beneficial to alga classifying pick-up unit 100 and can be placed in water and detects sample.
Optical signal prosessing assembly 2 can comprise photomultiplier or other photoelectric switching circuit, to realize the mutual conversion between light signal and electric signal.
Different exciting lights is sent to the sample in sample cell 3 by the light source that the plurality of centre wavelength is different, make sample because being produced corresponding fluorescence by different excitation, be converted to multi-channel electric signal through optical signal prosessing assembly 2, then carry out alga classifying detection by computing machine according to this multi-channel electric signal.
In the present embodiment, utilize as the different fluorescence molecules contained in the dissimilar algae of sample after by different excitation, obtain corresponding fluorescence by absorbing the energy of different exciting lights, and then utilize fluorescence excitation spectrum technology for detection principle to realize carrying out alga classifying detection to the multi-channel electric signal converted to by corresponding fluorescence by computing machine.
Because the light source that multiple centre wavelength is different can inspire multiple exciting light to sample simultaneously, the energy of the exciting light making the dissimilar algae Absorbable rod that comprises in sample different and draw corresponding fluorescence, carry out detecting to this corresponding fluorescence by computing machine and can detect dissimilar algae, thus avoid the too single problem of the algae type that simultaneously detects.
Refer to Fig. 2, in one embodiment, a kind of alga classifying pick-up unit 100 comprises interconnective light source assembly 1, sample cell 3 and optical signal prosessing assembly 2.
Particularly, sample cell 3 is T-shaped, and its small end is arranged with light source assembly 1, and is connected with optical signal prosessing assembly 2.This connected mode can for being threaded, riveting, weld or one-body molded.Preferably, the small end of sample cell 3 with optical signal prosessing assembly 2 for being threaded.
Further, between the junction of optical signal prosessing assembly 2 and light source assembly 1 and sample cell 3, be equipped with seal, to reach the object of waterproof.Preferably, the seal is fluid sealant, those junction adhesions are made by fluid sealant, even if thus make whole alga classifying pick-up unit 100 enter water also can not to have influence in light source assembly 1 and optical signal prosessing assembly 2 and intake, and only have water inlet in sample cell 3, thus still can realize the classification and Detection to sample in water.
Refer to Fig. 3, in one embodiment, the quantity of light source 11 is three.Preferably, three light sources 11 that centre wavelength is different are LED light source.
Further, the centre wavelength of three light sources 11 is respectively 590nm-610nm, 501nm-540nm and 455nm-490nm.Correspondingly, the color of the exciting light that first light source 11 sends is amber, and centre wavelength is 590nm-610nm; The color of the exciting light that second light source 11 sends is for green, and centre wavelength is 501nm-540nm; The color of the 3rd exciting light that light source 11 sends is for blue, and centre wavelength is 455nm-490nm.
Under the exciting of the exciting light sent at these three light sources 11, dissimilar algae can form different fluorescent effects, makes the sample including algae because being produced by those excitation the corresponding fluorescence that centre wavelength is roughly 680nm.
Further, light source assembly 1 also comprises a circular shell, and the plurality of light source 11 is evenly laid in circular shell.Wherein, the material of circular shell is the quartz of printing opacity waterproof.Certainly, in other embodiments, multiple light source 11 also can be laid in the same side in circular shell successively.
Further, modulated three light sources 11 by modulator-demodular unit, modulating frequency is respectively 700Hz, 900Hz and 1100Hz, is beneficial to the fluorescence spectrum detected easily by computing machine under corresponding modulation.That is, although the centre wavelength of the corresponding fluorescence produced by different excitation because of sample is unanimous on the whole, but its cyclical variation rule is consistent with the modulating frequency of the modulation suffered by corresponding exciting light, thus make the fluorescence spectrum that can be detected by computing machine under corresponding modulation, namely judge the classification of dissimilar algae.
Refer to Fig. 2 again, in one embodiment, optical signal prosessing assembly 2 comprises lens 21, filter plate 22 and photomultiplier 23 (PhotoMultiplierTube, PMT).Certainly, in other embodiments, photomultiplier 23 also can be substituted by other photoelectric switching circuit.
Particularly, fluorescence is after lens 21 optically focused, be incident to filtering parasitic light on filter plate 22, re-shoot to photomultiplier 23 and carry out opto-electronic conversion, with by highly sensitive, respond photomultiplier 23 rapidly the corresponding fluorescence after process be converted to multi-channel electric signal, and then make computing machine carry out alga classifying detection according to this multi-channel electric signal.
Further, the selected wavelength of filter plate 22 is 680nm.That is, wavelength is that parasitic light beyond 680nm cannot can by filtering by filter plate 22, the fluorescence produced because being excited optical excitation that wavelength is roughly 680nm then can not by filtering by filter plate 22, be beneficial to the detection of subsequent calculations machine to alga classifying, improve accuracy of detection.Certainly, in other embodiments, filter plate 22 also can by other filtering devices such as bandpass filter, low-pass filter or Hi-pass filters substitute.
Further, optical signal prosessing assembly 2 also comprises a tubular shell, and lens 21, filter plate 22 and photomultiplier 23 are uniform located therein.The material of this tubular shell is metal, and such as, this metal is the waterproof metal materials such as stainless steel.
In one embodiment, a kind of alga classifying pick-up unit also comprises data collecting card, between the photomultiplier that data collecting card is connected to optical signal prosessing assembly and computing machine, by data collecting card by conversion after multi-channel electric signal collection to computing machine, to carry out alga classifying detection by computing machine according to this multi-channel electric signal.
Wherein, the acquisition rate of data collecting card, to meet premised on testing requirement, is not limited it in the present embodiment.
Existing composition graphs 1 to Fig. 3, explained as follows to the principle of work of alga classifying pick-up unit 100 in a specific embodiment, optical path direction is as shown in figure chain lines.
(1) send modulated three different exciting lights by the LED light source of three in light source assembly 1 11 to surrounding, namely through the centre wavelength of 700Hz modulating frequency modulation be the first exciting light, the second exciting light sent through the centre wavelength of 900Hz modulating frequency modulation second LED light source 11 that be 520nm and the 3rd exciting light sent through the 3rd LED light source 11 that the centre wavelength of 1100Hz modulating frequency modulation is 470nm that first LED light source 11 of 590nm sends.
(2) sample made in sample cell 3 is excited and produces corresponding fluorescence by aforementioned three exciting lights, this corresponding fluorescence after lens 21 optically focused in optical signal prosessing assembly 2, filter plate 22 filtering parasitic light, then is converted into multi-channel electric signal through photomultiplier 23.
(3) be that the multi-channel electric signal collected is transferred to computing machine by the data collecting card of 50KHz by acquisition rate, processed this multi-channel electric signal by computing machine, the spectral line of the algae fluorescence excitation spectrum corresponding to the corresponding fluorescence gone out by three excitation that centre wavelength is different with basis and strength difference realization are to the classification and Detection of algae and quantitative measurment.
Foregoing, is only preferred embodiment of the present utility model, does not form the restriction to the utility model protection domain.Any do within spirit of the present utility model and principle amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (10)
1. an alga classifying pick-up unit, it is characterized in that, comprise light source assembly, sample cell and optical signal prosessing assembly, described light source assembly comprises the different light source of multiple centre wavelength, to be sent the sample in different exciting light to described sample cell by the plurality of different light source, make described sample because being produced corresponding fluorescence by described different excitation, be converted to multi-channel electric signal through described optical signal prosessing assembly, then carry out alga classifying detection by computing machine according to described multi-channel electric signal.
2. alga classifying pick-up unit as claimed in claim 1, it is characterized in that, described sample cell is T-shaped, and its small end is arranged with described light source assembly, and is connected with described optical signal prosessing assembly.
3. alga classifying pick-up unit as claimed in claim 1, is characterized in that, be equipped with seal between the junction of described optical signal prosessing assembly and light source assembly and described sample cell.
4. alga classifying pick-up unit as claimed in claim 1, it is characterized in that, the quantity of described light source is three.
5. alga classifying pick-up unit as claimed in claim 4, it is characterized in that, the centre wavelength of described three light sources is respectively 590nm-610nm, 501nm-540nm and 455nm-490nm.
6. alga classifying pick-up unit as claimed in claim 4, it is characterized in that, modulated described three light sources by modulator-demodular unit, modulating frequency is respectively 700Hz, 900Hz and 1100Hz.
7. alga classifying pick-up unit as claimed in claim 1, it is characterized in that, described light source assembly also comprises a circular shell, and described multiple light source is evenly laid in described circular shell, and the material of described circular shell is quartz.
8. alga classifying pick-up unit as claimed in claim 1, it is characterized in that, described optical signal prosessing assembly comprises lens, filter plate and photomultiplier, described fluorescence is after described lens light gathering, be incident to filtering parasitic light on described filter plate, re-shoot to described photomultiplier and carry out opto-electronic conversion.
9. alga classifying pick-up unit as claimed in claim 8, it is characterized in that, the selected wavelength of described filter plate is 680nm.
10. alga classifying pick-up unit as claimed in claim 8, it is characterized in that, described optical signal prosessing assembly also comprises a tubular shell, and described lens, filter plate and photomultiplier are uniform located therein, and the material of described tubular shell is metal.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520546821.5U CN204832039U (en) | 2015-07-26 | 2015-07-26 | Categorised detection device of alga |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520546821.5U CN204832039U (en) | 2015-07-26 | 2015-07-26 | Categorised detection device of alga |
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| CN204832039U true CN204832039U (en) | 2015-12-02 |
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| CN201520546821.5U Active CN204832039U (en) | 2015-07-26 | 2015-07-26 | Categorised detection device of alga |
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