CN204824905U - Do cell culture perfusion device - Google Patents
Do cell culture perfusion device Download PDFInfo
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- CN204824905U CN204824905U CN201520555022.4U CN201520555022U CN204824905U CN 204824905 U CN204824905 U CN 204824905U CN 201520555022 U CN201520555022 U CN 201520555022U CN 204824905 U CN204824905 U CN 204824905U
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- cell
- perfusion
- bottle
- cell culture
- stem cell
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Abstract
The utility model discloses a do cell culture perfusion device, including the perfusion bottle, raise a layer cell culture dish, cell perfusion groove and waste liquid bottle, perfusion bottle bottleneck department is equipped with first filter, the lateral wall of cell culture door is provided with a plurality of layers of slope inlet, and the incline direction of all slope inlets is unanimous, perfusion bottle 1 links to each other with the slope inlet through the input tube, being equipped with a first control ware that can show pH value and controlled temperature on the input tube, raising layer cell culture dish and link to each other with cell perfusion groove through first connecting pipe, be provided with the the second controllor and the first peristaltic pump that can show pH value and controlled temperature on the first connecting pipe, cell perfusion inslot is provided with screw feeder, is provided with electric heating wire inside the screw feeder. The utility model discloses utensil temperature control accuracy is high, and can improve the cultivation speed of cell, and that both can satisfy the stem cell independently cultivates the environment to guarantee cell purity, avoid again mixing and raise a layer cell influence.
Description
Technical field
The utility model relates to a kind of perfusion device, refers to a kind of stem cell culture perfusing device especially.
Background technology
The 1950's, Late Cambrian embryo is dry thin first in monster knurl for scientist, from then on to have started the course of stem cell biology research.The research and apply of stem cell almost relates to all life sciences and biomedical sector, is the focus and emphasis of current life science.The biological characteristics of stem cell energy self and Multidirectional Differentiation makes stem-cell research have a wide range of applications in the exploitation and clinical therapy of tumor etc. of animal cloning, new and effective medicine.
In embryonic stem cell vitro culture, except normal substratum, need first to cultivate the special cell of one deck (as mouse embryo fibroblast-mouseembryonicfibroblastMEF cell etc.), and then inoculate the stem cell that will test thereon.These special cells are exactly feeder layer cells, normally become fiber-like cell, these cells can secrete the necessary somatomedin of some the unknowns as leukaemia inhibitory factor, STEM CELL FACTOR and Prostatropin etc., provide embryonic stem cell, induced multi-potent stem cells better nutrition.The survival of these factor pair stem cells and propagation are required, and its quantity not only can be made to increase, and relevant with stem cell differentiation in vivo and in vitro.Experiment proves, the effect of feeder layer cells is not only create some cytokines, and in most cells growth and atomization, the adhesion of cell has all played considerable effect.ES cell can not be survived as completely independently cell, is always associated with the cell of surrounding, and the adhesion receptors in intracellular signaling regulates the motion of cell, propagation and differentiation.
Usually under embryonic stem cell/induced multi-potent stem cells culture system, feeder layer cells and stem cell are in Dual culture state, and both cannot be separated thoroughly.If adopt the mode of feeder layer and stem cell difference static cultivation, cannot in real time the factor that feeder layer cells is secreted be sent in stem cell culture system, and operating process is many, is scarcely used.Tradition Dual culture mode affects stem cell purity, in stem cell application etc., serves very large restriction, and all to there is temperature control accuracy low for existing equipment simultaneously, cultivates the defect that speed is lower.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of stem cell culture perfusing device, temperature control accuracy is high, and can improve the cultivation speed of cell, both can meet the independent culture environment of stem cell, to ensure cell purity, avoid again mixing feeder layer cells impact.
For solving the problems of the technologies described above, embodiment of the present utility model provides a kind of stem cell culture perfusing device, comprise perfusion bottle, feeder layer cells culture dish, cell perfusion groove and waste liquid bottle, perfusion bottle bottle mouth position is provided with the first filter, the sidewall of described cell cultures door is provided with some layers of inclination fluid inlet, the vergence direction of all inclination fluid inlets is consistent, described perfusion bottle 1 is connected with inclination fluid inlet by input tube, described input tube is provided with the first controller that can show pH value and control temperature, feeder layer cells culture dish is connected with cell perfusion groove by the first pipe connecting, first pipe connecting is provided with second controller and the first peristaltic pump that one can show pH value and control temperature, helical screw feeder is provided with in cell perfusion groove, helical screw feeder inside is provided with electrical heating wire, cell perfusion groove is connected with waste liquid bottle with the second peristaltic pump by the second pipe connecting.
As preferably, described waste liquid bottle is provided with the second filter.
As preferably, in described perfusion bottle, be provided with the PH sensor be connected with the first controller and heater strip.
As preferably, in described first pipe connecting, be provided with the PH sensor be connected with second controller and heater strip.
The utility model has following beneficial effect:
Temperature control accuracy is high, and can improve the cultivation speed of cell, both can meet the independent culture environment of stem cell, to ensure cell purity, avoids again mixing feeder layer cells impact.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model embodiment.
Embodiment
For making the technical problems to be solved in the utility model, technical scheme and advantage clearly, be described in detail below in conjunction with the accompanying drawings and the specific embodiments.
As shown in Figure 1, the utility model embodiment provides a kind of stem cell culture perfusing device, comprise perfusion bottle 1, feeder layer cells culture dish 5, cell perfusion groove 9 and waste liquid bottle 13, perfusion bottle 1 bottle mouth position is provided with the first filter 2, the sidewall of described cell cultures door 5 is provided with some layers of inclination fluid inlet 14, the vergence direction of all inclination fluid inlets 14 is consistent, described perfusion bottle 1 is connected with inclination fluid inlet 14 by input tube 3, described input tube 3 is provided with the first controller 4 that can show pH value and control temperature, feeder layer cells culture dish 5 is connected with cell perfusion groove 9 by the first pipe connecting 6, first pipe connecting 6 is provided with second controller 7 and the first peristaltic pump 8 that one can show pH value and control temperature, helical screw feeder 10 is provided with in cell perfusion groove 9, helical screw feeder 10 inside is provided with electrical heating wire 11, cell perfusion groove 9 is connected with waste liquid bottle 13 with the second peristaltic pump 12 by the second pipe connecting 16.
Described waste liquid bottle 13 is provided with the second filter 15.
The PH sensor and heater strip that are connected with the first controller 4 is provided with in described perfusion bottle 1.
The PH sensor and heater strip that are connected with second controller 7 is provided with in described first pipe connecting 6.
Wherein, described electrical heating wire 11 is connected with temperature control platform, described temperature control platform is arranged on the outer wall of cell perfusion groove 9, and the acute angle that described inclination fluid inlet 14 and the inwall of Tissue Culture Dish 5 are formed is 20 ° ~ 40 °, and the inclination fluid inlet 14 of different layers is crisscross arranged.
This concrete setting implemented by inclination fluid inlet 14, liquid is rotated, and temperature control accuracy is high, and can improve the cultivation speed of cell, both can meet the independent culture environment of stem cell, to ensure cell purity, avoids again mixing feeder layer cells impact.
The above is preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite not departing from principle described in the utility model; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. a stem cell culture perfusing device, comprise perfusion bottle (1), feeder layer cells culture dish (5), cell perfusion groove (9) and waste liquid bottle (13), perfusion bottle (1) bottle mouth position is provided with the first filter (2), it is characterized in that, the sidewall of described cell cultures door (5) is provided with some layers of inclination fluid inlet (14), the vergence direction of all inclination fluid inlets (14) is consistent, described perfusion bottle (1) is connected with inclination fluid inlet (14) by input tube (3), described input tube (3) is provided with the first controller (4) that can show pH value and control temperature, feeder layer cells culture dish (5) is connected with cell perfusion groove (9) by the first pipe connecting (6), first pipe connecting (6) is provided with second controller (7) and the first peristaltic pump (8) that one can show pH value and control temperature, helical screw feeder (10) is provided with in cell perfusion groove (9), helical screw feeder (10) inside is provided with electrical heating wire (11), cell perfusion groove (9) is connected with waste liquid bottle (13) with the second peristaltic pump (12) by the second pipe connecting (16).
2. a kind of stem cell culture perfusing device according to claim 1, is characterized in that, described waste liquid bottle (13) is provided with the second filter (15).
3. a kind of stem cell culture perfusing device according to claim 1, is characterized in that, is provided with the PH sensor be connected with the first controller (4) and heater strip in described perfusion bottle (1).
4. a kind of stem cell culture perfusing device according to claim 1, is characterized in that, is provided with the PH sensor be connected with second controller (7) and heater strip in described first pipe connecting (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520555022.4U CN204824905U (en) | 2015-07-24 | 2015-07-24 | Do cell culture perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520555022.4U CN204824905U (en) | 2015-07-24 | 2015-07-24 | Do cell culture perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204824905U true CN204824905U (en) | 2015-12-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520555022.4U Expired - Fee Related CN204824905U (en) | 2015-07-24 | 2015-07-24 | Do cell culture perfusion device |
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| CN (1) | CN204824905U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
-
2015
- 2015-07-24 CN CN201520555022.4U patent/CN204824905U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN111979088B (en) * | 2019-05-23 | 2022-08-26 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151202 Termination date: 20170724 |