CN103409319A - Tumor cell anoikis incubator - Google Patents
Tumor cell anoikis incubator Download PDFInfo
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- CN103409319A CN103409319A CN2013103149615A CN201310314961A CN103409319A CN 103409319 A CN103409319 A CN 103409319A CN 2013103149615 A CN2013103149615 A CN 2013103149615A CN 201310314961 A CN201310314961 A CN 201310314961A CN 103409319 A CN103409319 A CN 103409319A
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Abstract
The invention discloses a tumor cell anoikis incubator which comprises a 3D culture bracket filled with a cell culture medium and culture dishes arranged at two ends of the culture bracket; culture rooms filled with 3D materials are arranged in the culture dishes; the 3D culture bracket is communicated with the interiors of the culture dishes; an opening used for the cell culture medium to go in/out the culture rooms is formed in the culture room; the 3D culture bracket comprises two culture tanks oppositely arranged and at least one hollow spiral pipe for communicating the two culture tanks. The tumor cell anoikis incubator can simulate the in-vivo environment of a circulating tumor cell reliably, facilitate culturing the tumor cell and screening the anti-anoikis tumor cell, and provide an important experiment tool for studying tumor metastasis.
Description
Technical field
The invention belongs to cell cultures apparatus field, relate to a kind of tumour cell and lose the nest incubator.
Background technology
Anoikis, to disengage due to cell and extracellular matrix or flanking cell the apoptosis brought out, part tumour cell but can survive, become the Anoikis resistance cell, in the cell energy intravasation that wherein part is energetic, grade of malignancy is high, become circulating tumor cell, this part Anoikis resistance cell is realized the distant metastasis of tumour by blood circulation.
The research model of mechanism of tumor metastasis is confined to metastasis model in Mice Body at present, and cost is high, and the cycle is long.Not yet there is ripe external model to announce.Set up external model and at first will obtain the Anoikis resistance cell at experiment in vitro.At present, Effect of Anti anoikis model still is confined to agar plate culture, perhaps use simple external force to rock suspended culture cell, cause the cell can't adherent growth, can not simulate the true environment of metastases in human body, mainly there is following problems in this method: 1. this model is cultivated the Anoikis resistance cell obtained, and its survival condition can't analog cell and the impact of the contacting of extracellular matrix, flanking cell, local metabolic factor and blood vessel microenvironment factor; 2. the cell that obtains of this culture model is the state that is in static cultivation or rocks merely, and blood flow phase environment that can not analog cell existence, can not supply a model for the research of metastases.
Summary of the invention
In view of this, but the object of the present invention is to provide the tumour cell of metastases environment in a kind of analogue body to lose the nest incubator, this incubator can make tumour cell under the different treatment factor, from the dimensional culture support, break away from, enter the model that continues to cultivate and maintain its survival condition in flow system, the special equipment that provides cell in vitro to cultivate for the research Anoikis resistance of circulating tumor cell and mechanism of tumor metastasis, for the drug screening of target metastatic tumour cell provides research model.
For achieving the above object, the invention provides following technical scheme: comprise that inside is filled with the 3D cultivation support of cell culture medium and is arranged on the culture dish that 3D cultivates the support two ends, described culture dish is provided with the culturing room for the splendid attire three-dimensional material, described 3D cultivates support and communicates with culture dish inside, and described culturing room is provided with the opening for cell culture medium turnover culturing room; Described 3D cultivates support and comprises two corresponding culture tank and at least one solenoids that two culture tanks are connected that arrange.
Further, also comprise the connecting joint that culture tank and culture dish are linked together.
Further, also comprise and be arranged on 3D to cultivate support outside and 3D is cultivated to the protective guard of support in being enclosed in.
Further, described spiral tube is that pipe diameter size is the capillary blood vessel simulative tube of 50～300 μ m.
Beneficial effect of the present invention is: the internal milieu of simulation loop tumour cell preferably, the Anoikis resistance cell obtained is to break away from the cell of surviving after the contacting of cell and extracellular matrix or flanking cell, a group cell that not can't be adherent, this is identical with variation in body; Eliminated the defect of existing model, proved under blood flow, this group cell still can be survived or grade of malignancy changes, and can become the model of research metastases; Can cultivate on support or the material inwall at simulated blood vessel and apply processing factor Effect of Anti anoikis at 3D, simulated in vivo environment, provide important experimental tool for studying metastases preferably.
The accompanying drawing explanation
In order to make purpose of the present invention, technical scheme and beneficial effect clearer, the invention provides following accompanying drawing and describe:
Fig. 1 is structural representation of the present invention;
Fig. 2 is the structural representation that in the present invention, 3D cultivates support;
Wherein: 1-3D cultivates support, 2-culture dish, 3-culturing room, 4-culture tank, 5-spiral tube, 6-protective guard.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.
As shown in the figure, the tumour cell of the present embodiment loses the nest incubator and comprises that inside is filled with the 3D cultivation support 1 of cell culture medium and is connected to the culture dish 2 that 3D cultivates support 1 two ends, culture dish 2 has been provided with the inner splendid attire culturing room 3 of three-dimensional material, 3D cultivates support 1 and communicates with culture dish 2 inside, and culturing room 3 is provided with the opening for cell culture medium turnover culturing room 3; In the present embodiment, 3D cultivates taper culture tank 4 and two solenoids 5 that two culture tanks 4 are connected that support 1 comprises two corresponding settings; The tumour cell of good seal is lost to the nest incubator to be put upside down repeatedly in incubator, make current downflow on cell culture medium, the cell culture medium flowed enters in culturing room 3, allow and be attached to the Growth of Cells on three-dimensional material, the cell of having grown breaks away from from three-dimensional material, with the cell culture medium flowed, enter into flow system and fall in below taper culture tank 4, culture tank 4 communicates with culture dish 2, takes off culture dish 2 and can obtain required cell; Can simulate the environment of metastases in human body, true and reliable.
In the present embodiment, 3D cultivates support 1 and culture dish 2 be Split type structure, by connecting joint, culture tank 4 and culture dish 2 is linked together, and culture dish 2 can be cultivated support 1 from 3D and disassembles like this, is convenient to cycling and reutilization; Also available direct connection as an alternative solution, for example directly arranges connecting thread on culture tank 4 and culture dish 2, directly both is connected by screw thread, and certainly, 3D cultivates support 1 and culture dish 2 also can be structure as a whole.
In the present embodiment, also comprise and be arranged on 3D to cultivate support 1 outside and 3D is cultivated to the protective guard 6 of support 1 in being enclosed in; 3D cultivates the more tiny fragility of support 1, protective guard 6 is set and both had been convenient to protect 3D to cultivate support 1, facilitates again the testing crew operation.
In the present embodiment, be metastases environment in analogue body preferably, described spiral tube 5 simulation capillary blood vesseies, caliber is 50～300 μ m.
Finally explanation is, above preferred embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is described in detail by above preferred embodiment, but those skilled in the art are to be understood that, can to it, make various changes in the form and details, and not depart from the claims in the present invention book limited range.
Claims (4)
1. a tumour cell loses the nest incubator, it is characterized in that: comprise that inside is filled with the 3D cultivation support of cell culture medium and is arranged on the culture dish that 3D cultivates the support two ends, described culture dish is provided with the culturing room for the splendid attire three-dimensional material, described 3D cultivates support and communicates with culture dish inside, and described culturing room is provided with the opening for cell culture medium turnover culturing room; Described 3D cultivates support and comprises two corresponding culture tank and at least one solenoids that two culture tanks are connected that arrange.
2. tumour cell according to claim 1 loses the nest incubator, it is characterized in that: also comprise the connecting joint that culture tank and culture dish are linked together.
3. tumour cell according to claim 1 loses the nest incubator, it is characterized in that: also comprise being arranged on 3D cultivation support outside and 3D being cultivated to support being enclosed in interior protective guard.
4. tumour cell according to claim 1 loses the nest incubator, and it is characterized in that: described spiral tube is that pipe diameter size is the capillary blood vessel simulative tube of 50～300 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310314961.5A CN103409319B (en) | 2013-07-24 | 2013-07-24 | Tumor cell anoikis incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310314961.5A CN103409319B (en) | 2013-07-24 | 2013-07-24 | Tumor cell anoikis incubator |
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| Publication Number | Publication Date |
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| CN103409319A true CN103409319A (en) | 2013-11-27 |
| CN103409319B CN103409319B (en) | 2014-08-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310314961.5A Expired - Fee Related CN103409319B (en) | 2013-07-24 | 2013-07-24 | Tumor cell anoikis incubator |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109554296A (en) * | 2018-12-19 | 2019-04-02 | 南方医科大学第三附属医院（广东省骨科研究院） | A kind of in-vitro simulated circulating tumor cell generation device and its application |
| CN113373054A (en) * | 2021-05-26 | 2021-09-10 | 重庆大学附属肿瘤医院 | Cancer cell diffusion preference simulation device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4377639A (en) * | 1982-01-18 | 1983-03-22 | University Of Toledo | Tissue culture device for mass cell culture |
| CN201605273U (en) * | 2009-12-31 | 2010-10-13 | 邹绚 | Semi-adherent cell culture dish |
| CN201793567U (en) * | 2010-08-20 | 2011-04-13 | 明博医药技术开发（上海）有限公司 | Microorganism cultivation device |
| CN202175671U (en) * | 2011-08-08 | 2012-03-28 | 贵州省烟草科学研究所 | Humidity-adjustable microorganism incubator |
| CN202322812U (en) * | 2011-11-25 | 2012-07-11 | 上海师范大学 | Microorganism anaerobic culture tank |
-
2013
- 2013-07-24 CN CN201310314961.5A patent/CN103409319B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4377639A (en) * | 1982-01-18 | 1983-03-22 | University Of Toledo | Tissue culture device for mass cell culture |
| CN201605273U (en) * | 2009-12-31 | 2010-10-13 | 邹绚 | Semi-adherent cell culture dish |
| CN201793567U (en) * | 2010-08-20 | 2011-04-13 | 明博医药技术开发（上海）有限公司 | Microorganism cultivation device |
| CN202175671U (en) * | 2011-08-08 | 2012-03-28 | 贵州省烟草科学研究所 | Humidity-adjustable microorganism incubator |
| CN202322812U (en) * | 2011-11-25 | 2012-07-11 | 上海师范大学 | Microorganism anaerobic culture tank |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109554296A (en) * | 2018-12-19 | 2019-04-02 | 南方医科大学第三附属医院（广东省骨科研究院） | A kind of in-vitro simulated circulating tumor cell generation device and its application |
| CN109554296B (en) * | 2018-12-19 | 2024-03-26 | 南方医科大学第三附属医院（广东省骨科研究院） | In-vitro simulated circulation tumor cell generation device and application thereof |
| CN113373054A (en) * | 2021-05-26 | 2021-09-10 | 重庆大学附属肿瘤医院 | Cancer cell diffusion preference simulation device |
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| CN103409319B (en) | 2014-08-06 |
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