CN204807560U - Carry out outer driven immunity side direction chromatographic device to biological tag - Google Patents
Carry out outer driven immunity side direction chromatographic device to biological tag Download PDFInfo
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- CN204807560U CN204807560U CN201420852815.8U CN201420852815U CN204807560U CN 204807560 U CN204807560 U CN 204807560U CN 201420852815 U CN201420852815 U CN 201420852815U CN 204807560 U CN204807560 U CN 204807560U
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Abstract
The utility model discloses a carry out outer driven immunity side direction chromatographic device to biological tag installs including a carry out outer driven immunity side direction chromatographic device to biological tag, include by end liner 1, sample pad 2, combine to fill up 3, analysis membrane 4 and absorb water and fill up the 5 immune side direction chromatography test paper of constituteing, the analysis is epimembranal be provided with detect take 6 with matter the accuse take 7, still include upper reaches driving electrode 8 and low reaches driving electrode 9, the upper reaches driving electrode and the upper reaches outer ends of sample pad are connected, low reaches driving electrode and the low reaches outer ends of pad of absorbing water are connected. The utility model discloses a powerful and the even external driving force that the method adopted the electrode to produce carries out outer drive as " power supply " to springing up of biological tag to promote present each species immunity side direction chromatographic technique's accuracy, accuracy and measuring speed by a wide margin, and improved detection sensitivity through promoting biological tag to release on this basis.
Description
Technical field
The utility model belongs to vitro diagnostic techniques field, relates to a kind of immune lateral chromatography device biomarker being carried out to outer driving.
Background technology
Immunity lateral chromatography is a kind of field fast detection method the most classical in vitro diagnostic techniques field.The physics formation of immunity lateral chromatography test paper comprises three class materials: bioactive molecule, label, solid phase consumptive material.Its concrete inscape and function as follows:
Solid phase consumptive material: be multiple poromerics, can as the sample pad of immune lateral chromatography test paper, pad, analyzing film, adsorptive pads, viscosity end liner; The solidification of its thing bioactive molecule of making a living on the one hand provides solid phase support with bio-identification, and another aspect provides power by the capillary syphonic effect of microcellular structure for whole chromatography.
Bioactive molecule: be the multiple biomacromolecule with specific biological recognition capability, can be fixed on analyzing film and be fixed in pad as biomarker as detection zone and quality control band, with label coupling; It is the probe realizing specific biological identification in immunoassay procedures.
Label: be the large molecule of particle (comprising nanometer, sub-micron etc.) or chemistry (comprise organic and inorganic etc.) with characteristics such as optical, electrical, magnetic, colors, can be fixed in pad as biomarker with bioactive molecule coupling; The characteristics such as himself optical, electrical, magnetic, color, the bioactive molecule that can carry out microcosmic and detect specific biological identification between target and give macroscopic view and show.
Bioactive molecule in immune lateral chromatography detection paper process, relation imbody between label and solid phase consumptive material is as follows, under the capillary siphon power of solid phase consumptive material, the biomarker prestored in solid phase material (pad) is released under the effect of fluid sample, bioactive molecule (detection zone) place fixing in advance to solid phase material (analyzing film) is sprung up with sample, and there is " bioactive molecule of coupling on biomarker " at this place, " detection target ", bio-identification special between " bioactive molecule fixing in detection zone ", this bio-identification can change the amount of this place's label by the bioactive molecule in biomarker, thus by the light of label, electricity, magnetic, the characteristics such as color give qualitative or quantitative displaying to bio-identification.
The specific recognition of bioactive molecule is the fact of outwardness, when bioactive molecule characteristic (compatibility, specificity) is clear and definite, immune lateral chromatography technology is exactly the carrier fact of bioactive molecule specific recognition being given to displaying objective, directly perceived.As a kind of display carrier, immune lateral chromatography technology mainly deposits problem both ways in actual use, first identification show susceptibility, quantize ability, its two be identify show accuracy, accuracy.
With regard to susceptibility, quantification ability, main relevant with label self-characteristic, the i.e. easy identification of optical, electrical, the characteristic such as magnetic, color of label and quantification property, the various immune lateral chromatography method of prior art, if colloid gold immune lateral chromatography, fluorescence immunoassay lateral chromatography, paramagnetic particle immunity lateral chromatography are all concentrate on to carry out screen and optimizes to various tracer, thus improve the detection sensitivity of immune lateral chromatography and realization detection target quantification.
And with regard to accuracy, accuracy, mainly with biomarker under solid phase consumptive material capillary action to spring up state relevant, namely the homogeneity of solid phase consumptive material self microscopic aperture and generation capillary syphonic effect thereof, springs up the impact of speed and homogeneity on biomarker.But immune lateral chromatography solid phase consumptive material production technology determines its microscopic aperture and distribution certainly exists great unevenness, result also in current chromatography product thus and all face the larger problem of testing result variation; Faint capillary siphon power also makes lateral chromatography required time relatively grow (about 10-15 minute) simultaneously.
Utility model content
The purpose of this utility model overcomes the deficiencies in the prior art, provides a kind of structure simply biomarker to be carried out to the immune lateral chromatography device of outer driving.
The technical solution of the utility model is summarized as follows:
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, described upstream drive electrode is connected with the upstream outer end of sample pad, or upstream drive electrode is by the lower surface of upstream drive electrode and the upper surface of sample pad, or the upper surface of pad, or the analyzing film upper surface of detection zone upstream or sample pad, pad, the upper surface of the analyzing film of detection zone upstream connects, described downstream drive electrode is connected with the downstream outboard end of adsorptive pads or downstream drive electrode is connected by the upper surface of the analyzing film in the upper surface of the analyzing film in the lower surface of downstream drive electrode and the upper surface of adsorptive pads or quality control band downstream or adsorptive pads, quality control band downstream.
Described immune lateral chromatography test paper is the immune lateral chromatography test paper using coloured particle as label, the immune lateral chromatography test paper using fluorescent macromolecule as label, the immune lateral chromatography test paper using fluorescent grain as label or the immune lateral chromatography test paper using paramagnetic particle as label.
The described immune lateral chromatography test paper using coloured particle as label is colloid gold immune lateral chromatography test paper or latex beads immunity lateral chromatography test paper.
The described immune lateral chromatography test paper using fluorescent macromolecule as label is Cy5 fluorescence immunoassay lateral chromatography test paper, Cy3 fluorescence immunoassay lateral chromatography test paper, FITC fluorescence immunoassay lateral chromatography test paper or green fluorescent protein immunity lateral chromatography test paper.
The described immune lateral chromatography test paper using fluorescent grain as label is quantum dot immune lateral chromatography test paper, on turn fluorescence immunoassay lateral chromatography test paper or fluorescent microsphere immunity lateral chromatography test paper.
Advantage of the present utility model:
The utility model, overcome prior art using immune lateral chromatography solid phase consumptive material simultaneously as " carrier " and " power source " of bio-identification time, solid phase consumptive material microcellular structure heterogeneity, capillary siphon power is faint cause " power source " drive lower biomarker spring up uneven, speed is comparatively slow, thus the problem such as the testing result accuracy caused is poor, detection speed is slow.Based on the utility model, the function of immunochromatography solid phase consumptive material is only positioned as " carrier " of bio-identification, and the more powerful and uniform external driving force adopting electrode to produce carries out outer driving as " power source " to springing up of biomarker, thus significantly improve the accuracy of existing various immune lateral chromatography technology, accuracy and detection speed, and on this basis by promoting that biomarker release improves detection sensitivity.Finally, meet on the one hand the demand of user for immune lateral chromatography test paper more excellent technique performance, also reduce the difficulty of manufacturing enterprise's quality control on the other hand.
Accompanying drawing explanation
Fig. 1 is the immune lateral chromatography test paper of prior art.
Fig. 2 is of the present utility model a kind of to one of biomarker immune lateral chromatography device carrying out outer driving.
Fig. 3 is a kind of immune lateral chromatography device two biomarker being carried out to outer driving of the present utility model.
Fig. 4 is a kind of immune lateral chromatography device three biomarker being carried out to outer driving of the present utility model.
Fig. 5 is a kind of immune lateral chromatography device four biomarker being carried out to outer driving of the present utility model.
Fig. 6 is a kind of immune lateral chromatography device five biomarker being carried out to outer driving of the present utility model.
Fig. 7 is a kind of immune lateral chromatography device six biomarker being carried out to outer driving of the present utility model.
Fig. 8 is a kind of immune lateral chromatography device seven biomarker being carried out to outer driving of the present utility model.
Fig. 9 is a kind of immune lateral chromatography device eight biomarker being carried out to outer driving of the present utility model.
Figure 10 is a kind of immune lateral chromatography device nine biomarker being carried out to outer driving of the present utility model.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is further described.
Embodiment 1
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described immune lateral chromatography test paper is belong to the colloid gold immune lateral chromatography test paper in the immune lateral chromatography test paper using coloured particle as label, described analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, described upstream drive electrode is connected with the upstream outer end of sample pad, described downstream drive electrode is connected with the downstream outboard end of adsorptive pads.As shown in Figure 2.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, be energized respectively to upstream drive electrode 8 and downstream drive electrode 9, naked eyes carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 2
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, described analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upstream outer end of sample pad, downstream drive electrode is connected with the downstream outboard end of adsorptive pads.As shown in Figure 2.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 3
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described immune lateral chromatography test paper is the Cy5 fluorescence immunoassay lateral chromatography test paper belonged in the immune lateral chromatography test paper using fluorescent macromolecule as label, described analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upstream outer end of sample pad, downstream drive electrode is connected with the downstream outboard end of adsorptive pads.As shown in Figure 2.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 4
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described immune lateral chromatography test paper is the paramagnetic particle immunity lateral chromatography test paper belonged in the immune lateral chromatography test paper using paramagnetic particle as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upstream outer end of sample pad, downstream drive electrode is connected with the downstream outboard end of adsorptive pads.As shown in Figure 2.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt magnetometric analysis instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 5
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of sample pad by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the downstream outboard end of adsorptive pads.As shown in Figure 3.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 6
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of sample pad by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 4.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 7
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of sample pad by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the upper surface of the analyzing film in quality control band downstream by the lower surface of downstream drive electrode.As shown in Figure 5.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 8
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of sample pad by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the analyzing film in quality control band downstream and the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 6.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 9
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upstream outer end of print pad; Downstream drive electrode is connected with the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 7.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 10
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of pad by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 8.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 11
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise the immune lateral chromatography test paper be made up of end liner 1, sample pad 2, pad 3, analyzing film 4 and adsorptive pads 5, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode is connected with the upper surface of the analyzing film of detection zone upstream by the lower surface of upstream drive electrode; Downstream drive electrode is connected with the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 9.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Embodiment 12
A kind of immune lateral chromatography device biomarker being carried out to outer driving, comprise by end liner 1, sample pad 2, pad 3, the immune lateral chromatography test paper that analyzing film 4 and adsorptive pads 5 form, described immune lateral chromatography test paper is belong to the quantum dot immune lateral chromatography test paper in the immune lateral chromatography test paper using fluorescent grain as label, analyzing film is provided with detection zone 6 and quality control band 7, also comprise upstream drive electrode 8 and downstream drive electrode 9, upstream drive electrode passes through lower surface and the sample pad of upstream drive electrode, pad, the upper surface of the analyzing film of detection zone upstream connects, downstream drive electrode is connected with the upper surface of adsorptive pads by the lower surface of downstream drive electrode.As shown in Figure 10.
With a kind of of the present embodiment, the immune lateral chromatography device that biomarker carries out outer driving is detected crystal methamphetamine in urine:
In sample pad 2, add detected sample, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized, adopt optic analytical instrument to carry out result interpretation to the detection zone 6 on analyzing film 4 and quality control band 7.
Experimental example 1: the effectiveness comparison biomarker of different test paper being carried out to outer driving
[experimental implementation]:
1. the device of colloid gold immune lateral chromatography test paper and embodiment 1 drives effect control experiment:
Colloid gold immune lateral chromatography test paper: be added in test paper sample pad by the urine 100 μ l/ bar containing crystal methamphetamine 1500ng/ml, 1000ng/ml, 500ng/ml, 100ng/ml, 50ng/ml, 0ng/ml, leaves standstill observations; Each concentration determination 3 times;
The device of embodiment 1: the urine 100 μ l/ bar containing crystal methamphetamine 1500ng/ml, 1000ng/ml, 500ng/ml, 100ng/ml, 50ng/ml, 0ng/ml is added in test paper sample pad 2, respectively upstream drive electrode 8 and downstream drive electrode 9 are energized (turn on angle is 10V), leave standstill observations; Each concentration determination 3 times; In table 1.
2. the device of quantum dot immune lateral chromatography test paper and embodiment 2 drives effect control experiment:
Quantum dot immune lateral chromatography test paper: be added in test paper sample pad by the urine 100 μ l/ bar containing crystal methamphetamine 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1ng/ml, 0ng/ml, leaves standstill, optical analyser analysis result; Each concentration determination 3 times;
The device of embodiment 2: the urine 100 μ l/ bar containing crystal methamphetamine 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1ng/ml, 0ng/ml is added in test paper sample pad 2, respectively (turn on angle is 10V) is energized to upstream drive electrode 8 and downstream drive electrode 9, leave standstill, optical analyser analysis result; Each concentration determination 3 times; In table 1.
Cy5 fluorescence immunoassay lateral chromatography test paper drives effect control experiment:
Cy5 fluorescence immunoassay lateral chromatography test paper: the urine 100 μ l/ bar containing crystal methamphetamine 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1ng/ml, 0ng/ml is added in test paper sample pad, leave standstill, optical analyser analysis result; Each concentration determination 3 times;
The device of embodiment 3: the urine 100 μ l/ bar containing crystal methamphetamine 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 2.5ng/ml, 1ng/ml, 0ng/ml is added in test paper sample pad 2, respectively (turn on angle is 10V) is energized to upstream drive electrode 8 and downstream drive electrode 9, leave standstill, optical analyser analysis result; Each concentration determination 3 times; In table 1.
Paramagnetic particle immunity lateral chromatography test paper drives effect control experiment:
Paramagnetic particle immunity lateral chromatography test paper: the urine 100 μ l/ bar containing crystal methamphetamine 150ng/ml, 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 0ng/ml is added in test paper sample pad, leaves standstill, magnetic analytical meter analysis result; Each concentration determination 3 times;
The device of embodiment 4: by containing crystal methamphetamine 150ng/ml, 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, the urine 100 μ l/ bar of 0ng/ml is added in test paper sample pad 2, respectively (turn on angle is 10V) is energized to upstream drive electrode 8 and downstream drive electrode 9, leave standstill, magnetic analytical meter analysis result; Each concentration determination 3 times; In table 1.
Interpretation of result:
Detection time: to the clear and definite positive findings of appearance from interpolation sample, the required time;
Susceptibility: the least concentration that can show clear and definite positive findings is detectability, represents detection sensitivity;
Accuracy: the sample of detectability corresponding concentration, the coefficient of variation (CV=SD/Mean) of three measurement results, represents accuracy;
Accuracy: the sample of detectability corresponding concentration, the number percent (recovery=measurement concentration/theoretical concentration) of measurement result and theoretical configuration concentration, represents accuracy;
[results and analysis]:
Table 1
Detection time: add after electrode drives outward, no matter which kind of foreshortens to 3min by 15min detection paper time;
Susceptibility: after interpolation electrode drives outward, no matter which kind of detection paper susceptibility all has remarkable lifting, promotes minimum 10 times of amplitude;
Accuracy: after interpolation electrode drives outward, the detection accuracy of quantitative testing test paper all significantly promotes;
Accuracy: after interpolation electrode drives outward, the detection accuracy of quantitative testing test paper all significantly promotes;
Experimental example 2: quantum dot immune lateral chromatography test paper (fluorescent grain immunity lateral chromatography test paper) arranges the effectiveness comparison of electrode at diverse location
[experimental implementation]:
Respectively the urine 100 μ l/ bar containing crystal methamphetamine 10ng/ml, 5ng/ml, 2.5ng/ml, 1ng/ml, 0ng/ml is added in the sample pad 2 of the device of one of embodiment 5-12; Be energized (turn on angle is 10V) to upstream drive electrode 8 and downstream drive electrode 9 respectively, leave standstill, optical analyser analysis result is in table 2; Each concentration determination 3 times;
Interpretation of result:
Detection time: to the clear and definite positive findings of appearance from interpolation sample, the required time;
Susceptibility: the least concentration that can show clear and definite positive findings is detectability, represents detection sensitivity;
Accuracy: the sample of detectability corresponding concentration, the coefficient of variation (CV=SD/Mean) of three measurement results, represents accuracy;
Accuracy: the sample of detectability corresponding concentration, the number percent (recovery=measurement concentration/theoretical concentration) of measurement result and theoretical configuration concentration, represents accuracy;
[results and analysis]: in table 2
Table 2
| Embodiment | Detection time | Detectability (susceptibility) | The coefficient of variation (accuracy) | The recovery (accuracy) |
| 5 | 3min | 2.5ng/ml | 3.2％ | 104％ |
| 6 | 3min | 2.5ng/ml | 3.3％ | 102％ |
| 7 | 3min | 2.5ng/ml | 3.5％ | 104％ |
| 8 | 3min | 2.5ng/ml | 3.7％ | 102％ |
| 9 | 3min | 2.5ng/ml | 3.1％ | 102％ |
| 10 | 3min | 2.5ng/ml | 3.2％ | 105％ |
| 11 | 3min | 2.5ng/ml | 3.1％ | 104％ |
| 12 | 3min | 2.5ng/ml | 3.7％ | 102％ |
Detection time: no matter which position electrode is arranged on, and all can complete detection in 3min;
Susceptibility: no matter which position electrode is arranged on, and detection sensitivity all can reach 2.5ng/ml;
Accuracy: no matter which position electrode is arranged on, and accuracy does not have marked difference;
Accuracy: no matter which position electrode is arranged on, and accuracy does not have marked difference;
Above embodiment and experimental example are all illustrate one of the present utility model, the restriction not to the utility model technology contents.The position of upstream drive electrode and downstream drive electrode can combination in any, is not limited to the above-mentioned mode listed.For magnetic particle immunity lateral chromatography test paper, drive electrode can be changed to magnet and drive magnetic granular biological label.
In actual applications, dripped in sample pad 2 by the fluid sample containing detected target, sample permeates enters pad 3 and is taken out of by the biomarker wherein solidified; And then, fluid sample and biomarker potpourri spring up under the drive of solid phase material capillary siphon " power source " on analyzing film 4, and the specific recognition occurred when passing through detection zone 6 and quality control band 7 between " bioactive molecule in biomarker ", " detected target ", " bioactive molecule in detection zone " is reacted, thus biomarker is fixed on detection zone 6 with on quality control band 7, and label in fixing biomarker just the characteristic such as light, point, magnetic of its uniqueness can give qualitative or quantitative displaying to bio-identification.
Pass through the utility model, in upstream and the downstream of immune lateral chromatography test paper, connect upstream drive electrode 8 and downstream drive electrode 9 respectively, biomarker can be made more even springing up of test paper inside (especially on analyzing film), quick, thus improve the accuracy, the accuracy that detect; And the effect of external driving force also can make more biomarker discharge from pad, and then increase the susceptibility detected.
Claims (5)
1. one kind is carried out the immune lateral chromatography device of outer driving to biomarker, comprise by end liner (1), sample pad (2), pad (3), the immune lateral chromatography test paper that analyzing film (4) and adsorptive pads (5) form, described analyzing film is provided with detection zone (6) and quality control band (7), it is characterized in that also comprising upstream drive electrode (8) and downstream drive electrode (9), described upstream drive electrode is connected with the upstream outer end of sample pad, or upstream drive electrode is by the lower surface of upstream drive electrode and the upper surface of sample pad, or the upper surface of pad, or the analyzing film upper surface of detection zone upstream or sample pad, pad, the upper surface of the analyzing film of detection zone upstream connects, described downstream drive electrode is connected with the downstream outboard end of adsorptive pads or downstream drive electrode is connected by the upper surface of the analyzing film in the upper surface of the analyzing film in the lower surface of downstream drive electrode and the upper surface of adsorptive pads or quality control band downstream or adsorptive pads, quality control band downstream.
2. a kind of immune lateral chromatography device biomarker being carried out to outer driving according to claim 1, is characterized in that described immune lateral chromatography test paper is the immune lateral chromatography test paper using coloured particle as label, the immune lateral chromatography test paper using fluorescent macromolecule as label, the immune lateral chromatography test paper using fluorescent grain as label or the immune lateral chromatography test paper using paramagnetic particle as label.
3. a kind of immune lateral chromatography device biomarker being carried out to outer driving according to claim 2, is characterized in that the described immune lateral chromatography test paper using coloured particle as label is for colloid gold immune lateral chromatography test paper or latex beads immunity lateral chromatography test paper.
4. a kind of immune lateral chromatography device biomarker being carried out to outer driving according to claim 2, is characterized in that the described immune lateral chromatography test paper using fluorescent macromolecule as label is Cy5 fluorescence immunoassay lateral chromatography test paper, Cy3 fluorescence immunoassay lateral chromatography test paper, FITC fluorescence immunoassay lateral chromatography test paper or green fluorescent protein immunity lateral chromatography test paper.
5. a kind of immune lateral chromatography device biomarker being carried out to outer driving according to claim 2, it is characterized in that the described immune lateral chromatography test paper using fluorescent grain as label be quantum dot immune lateral chromatography test paper, on turn fluorescence immunoassay lateral chromatography test paper or fluorescent microsphere immunity lateral chromatography test paper.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105807042A (en) * | 2014-12-29 | 2016-07-27 | 张红 | Biomarker outer driving immune lateral chromatography device and method |
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| CN105807042A (en) * | 2014-12-29 | 2016-07-27 | 张红 | Biomarker outer driving immune lateral chromatography device and method |
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