CN207401491U - For the micro-fluidic chemical luminous chip of cell tests - Google Patents
For the micro-fluidic chemical luminous chip of cell tests Download PDFInfo
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- CN207401491U CN207401491U CN201721088476.0U CN201721088476U CN207401491U CN 207401491 U CN207401491 U CN 207401491U CN 201721088476 U CN201721088476 U CN 201721088476U CN 207401491 U CN207401491 U CN 207401491U
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Abstract
The utility model discloses a kind of micro-fluidic chemical luminous chips for cell tests, including chemical illuminating reagent and sample implanted layer, cell culture layer and glass substrate layer, at least one cell culture microchannel is distributed on cell culture layer, chemical illuminating reagent and sample implanted layer both sides set cell culture fluid import and cell culture fluid outlet, at least one chemical illuminating reagent and sample is set to inject microchannel on chemical illuminating reagent and sample implanted layer, there are square crossing connectivity points in chemical illuminating reagent and sample injection microchannel with cell culture microchannel, the both ends of the upside chemical illuminating reagent and sample of chemical illuminating reagent and sample implanted layer injection microchannel are respectively arranged with chemical illuminating reagent and sample injection silicagel column.Micro-fluidic chip with chemical luminescence detection method is combined, realizes the biochemical reaction between human body environment simulation, cell culture, cell line and chemical reaction integration, realize on chip piece simultaneously multiple data quantity, highly sensitive detect and results contrast.
Description
Technical field
The utility model is related to a kind of multi-layer micro-fluidic chips, more particularly to for cell culture and the miniflow of cell tests
Chemical luminous chip is controlled, belongs to biochip field.
Background technology
The basic operation units such as cell culture, reaction and detection can be integrated into core as small as possible by microfluidic chip technology
On piece, and be equipped with detection device realizes integration operation, provides one for culture and the test of cell and extremely important puts down
Platform.Micro-fluidic chip as the carrier of microfluidic chip technology may be designed to different dimensions, multimode communication configuration, seal in addition
The environment closed can simulated in vivo environment, realize on piece cell culture, separation or other processing, being aided with corresponding detector can obtain
Obtain various index parameters.Micro-fluidic chip has detector the special requirement such as high sensitivity, fast response time.Optical detection
Due to it is simple, interference is small and is widely used.The optical detection used on chip mainly includes fluorescence method and chemoluminescence method.
Wherein chemoluminescence method is not required to additional light source, has many advantages, such as that low background signal, high sensitivity and equipment are simple, becomes common
One of detection means.
204536292 U of patent document CN disclose a kind of biological detection chip, including porous plate and arranged on porous plate
Probe dot matrix in hole utilizes immunoassay detection antibiotic, illegal additive and biotoxin.Chip described in the patent is
A kind of chip of molecular level detection, and unbonded fluorogenic chemiluminescence instrument, realize integration detection.Patent document CN
103801412 A disclose a kind of integrated micro-flow control chip for enzymatic product fluoroscopic examination, integrate difference in functionality, can
With the acquisition that analysis data are carried out in the microplate reader of standard.Although chip disclosed in the patent can be carried out with microplate reader matching
Pattern detection, but equally in molecular level, and method therefor is fluoroscopic examination, there are background signal interference.Patent document CN
103344759 A disclose a kind of single highly sensitive detection aflatoxin of pearl photon crystal micro-ball liquid-phase chip chemoluminescence method
The method of B1, is combined by antigen and antibody specific, and chemiluminescence signal is detected using multi-function microplate reader.The patent
Although disclosed method is also compatible with microplate reader, molecular level detection is similarly.
Chemiluminescence with micro-fluidic chip is combined at present, it is Fluoresceinated to build micro-fluidic chemical luminous chip replacement commercialization
Learn the micropore board detector orifice plate of shining, continuously cultivated for cell with cell tests not yet it has been reported that.Therefore, build new
Micro-fluidic chemical luminous chip, realize cell culture with it is test integrated be very necessary.
Utility model content
In order to solve the above-mentioned technical problem, the utility model provides a kind of micro-fluidic chemiluminescence core for cell tests
Piece is combined micro-fluidic chip with chemical luminescence detection method, realizes the life between human body environment's simulation, cell culture, cell line
Change reaction and chemical reaction integration, realize on chip piece multiple data quantity, highly sensitive detection and results contrast simultaneously.
To achieve these goals, the technical solution of the utility model is as follows：A kind of micro-fluidicization for cell tests
Learn luminescence chip, it is characterised in that：Including stacking the chemical illuminating reagent of setting and sample implanted layer, cell successively from top to bottom
Culture layer and glass substrate layer, cross direction profiles have at least one cell culture microchannel, the chemistry on the cell culture layer
Luminescence reagent and sample implanted layer both sides correspond to every cell culture microchannel both ends set respectively axis up and down extend it is thin
The import of born of the same parents' culture solution and cell culture fluid outlet, the cell culture fluid import and cell culture fluid outlet and cell culture are micro- logical
Road communicates, the aperture of the upside cell culture fluid import of the chemical illuminating reagent and sample implanted layer and cell culture fluid outlet
On be provided with cell culture fluid injection silicagel column, the cell training that axis extends up and down is provided on the cell culture fluid silicagel column
Nutrient solution injection hole；
Also longitudinally disposed at least one chemical illuminating reagent and sample note on the chemical illuminating reagent and sample implanted layer
Enter microchannel, the chemical illuminating reagent and sample injection microchannel are located between the cell culture fluid silicagel column of both sides, described
Chemical illuminating reagent and sample injection microchannel have with cell culture microchannel intersects connectivity points, the chemical illuminating reagent and sample
The both ends of upside chemical illuminating reagent and sample the injection microchannel of this implanted layer are respectively arranged with chemical illuminating reagent and sample
Silicagel column is injected, is provided on the chemical illuminating reagent and sample injection silicagel column micro- with chemical illuminating reagent and sample injection
The solution injection hole of passage unicom.
In use, after micro-fluidic chip carries out sterilization treatment, pumped by flow injection and train cell suspending liquid by cell
Nutrient solution injection silicagel column is slowly injected into cell culture passage, makes cell evenly laid out in cell culture passage, in incubator
Cellar culture.Cell is passed through the thickness of various concentration from cell culture fluid injection silicagel column after ABAP oxidative stress induction processing
Plain phenol/honokiol solution, continues to be incubated, and removes magnolol/honokiol solution in passage.
Chemical illuminating reagent and sample injection microchannel and the connectivity points of intersecting of cell culture microchannel are examined for chemiluminescence
Survey window.The detection of optical signalling is detected by fluorogenic chemiluminescence instrument on chip.Start fluorogenic chemiluminescence instrument, chemistry is sent out
Light detection window alignment detection hole center, by flow injection pump, chemically luminescence reagent and sample inject silicagel column to chemistry hair
Luminol solution is injected in light reagent and sample injection microchannel, and records chemiluminescence signal.The results show that micro-fluidicization
The culture and test of on piece cell can be realized by learning luminescence chip；Magnolol/honokiol is respectively provided with apparent antioxidation activity.
In said program：The chemical illuminating reagent and sample implanted layer, cell culture layer are made of polymer material.
In said program：The cell culture microchannel is 1-16 items, the spacing between every two cell culture microchannels
For 1-10mm.50~150 μm of the depth of the cell culture microchannel, 250~350 μm wide, long 15~20mm.The chemistry hair
Light reagent and sample injection microchannel are 1-24 items, every chemical illuminating reagent and sample injection microchannel and every cell culture
There are intersection connectivity points in microchannel.The chemical illuminating reagent and sample injection microchannel deep 50~150 μm, wide 250~350 μ
M, long 25~40mm, the spacing between every two chemical illuminating reagents and sample injection microchannel is 1~10mm.Realize one piece of core
On piece multiple data quantity, highly sensitive detection.
Advantageous effect：The micro-fluidic chemical luminous chip for cell tests of the utility model is by micro-fluidic chip with changing
It learns luminous detection method to combine, realizes biochemical reaction and chemical reaction one between human body environment's simulation, cell culture, cell line
Change, realize on chip piece multiple data quantity, highly sensitive detection and results contrast simultaneously.Fungible goods orifice plate is realized glimmering
Integrated, integral application of the photochemiluminescence micropore board detector in terms of cell tests.
Description of the drawings
Fig. 1 is the structure diagram of the utility model.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples：
Embodiment 1, as shown in Figure 1：The micro-fluidic chemical luminous chip for cell tests of the utility model is by chemistry
Luminescence reagent and sample implanted layer 1, cell culture layer 2, glass substrate layer 3, cell culture microchannel 4, chemistry meeting luminescence reagent
And sample injection microchannel 5, cell culture fluid silicagel column 6, chemical illuminating reagent and sample injection silicagel column 7 form.
Chemical illuminating reagent and sample implanted layer 1, cell culture layer 2 and glass substrate layer 3 stack successively from top to bottom, change
It learns luminescence reagent and sample implanted layer 1, cell culture layer 2 is prepared by polymer material by formpiston.
Cross direction profiles have at least one cell culture microchannel 4 on cell culture layer 2, and preferred cell culture microchannel 4 is
1-16 items, all cell culture microchannels 4 are parallel, and the spacing between every two cell culture microchannels 4 is 1-10mm.Cell is trained
Support 50~150 μm of the depth of microchannel 4,250~350 μm wide, long 15~20mm.
The both ends that chemical illuminating reagent and 1 both sides of sample implanted layer correspond to every cell culture microchannel 4 set axis respectively
The cell culture fluid import and cell culture fluid outlet that line extends up and down, cell culture fluid import and cell culture fluid outlet with it is thin
Born of the same parents cultivate microchannel 4 and communicate, and the upside cell culture fluid import of chemical illuminating reagent and sample implanted layer 1 and cell culture fluid go out
Cell culture fluid injection silicagel column 6 is provided on the aperture of mouth, is provided with what axis extended up and down on cell culture fluid silicagel column 6
Cell liquid injection hole, the center line of cell culture fluid injection hole and the center line of cell culture fluid import or cell culture fluid outlet
On the same line, cell suspending liquid etc. injects silicagel column 6 by cell culture fluid and injects in cell culture microchannel 4.
Also longitudinally disposed at least one chemical illuminating reagent and sample injection are micro- on chemical illuminating reagent and sample implanted layer 1
Passage 5, as needed, chemical illuminating reagent and sample injection microchannel 5 are 1-24 items, all chemical illuminating reagents and sample note
It is parallel to enter microchannel 5, chemical illuminating reagent and sample injection microchannel 5 are 50~150 μm deep, 250~350 μm wide, long by 25~
40mm, the spacing between every two chemical illuminating reagents and sample injection microchannel 5 is 1~10mm.Chemical illuminating reagent and sample
This injection microchannel 5 is located between the cell culture fluid silicagel column 6 of both sides, every chemical illuminating reagent and sample injection microchannel
5 have with every cell culture microchannel 4 and intersect connectivity points.The upside chemiluminescence of chemical illuminating reagent and sample implanted layer 1
The both ends of reagent and sample injection microchannel 5 are respectively arranged with chemical illuminating reagent and sample injection silicagel column 7, chemiluminescence examination
The solution injection hole with chemical illuminating reagent and sample injection 5 unicom of microchannel is provided in agent and sample injection silicagel column 7.
The utility model is not limited to above-mentioned specific embodiment, it should be understood that those of ordinary skill in the art are without wound
The property made work, which according to the present utility model can conceive, makes many modifications and variations.In short, all technologies in the art
Personnel can be obtained according to the design of the utility model by logical analysis, reasoning, or a limited experiment on the basis of existing technology
The technical solution arrived, all should be in the protection domain being defined in the patent claims.
Claims (6)
1. a kind of micro-fluidic chemical luminous chip for cell tests, it is characterised in that：It is set including stacking successively from top to bottom
Chemical illuminating reagent and sample implanted layer (1), cell culture layer (2) and the glass substrate layer (3) put, the cell culture layer
(2) cross direction profiles have at least one cell culture microchannel (4), the chemical illuminating reagent and sample implanted layer (1) both sides on
The both ends of corresponding every cell culture microchannel (4) set the cell culture fluid import and cell culture that axis extends up and down respectively
Liquid exports, and the cell culture fluid import and cell culture fluid outlet are communicated with cell culture microchannel (4), the chemiluminescence
Cell culture is provided on the aperture of the upside cell culture fluid import of reagent and sample implanted layer (1) and cell culture fluid outlet
Liquid injects silicagel column (6), and the cell culture fluid injection that axis extends up and down is provided on the cell culture fluid silicagel column (6)
Hole；
Also longitudinally disposed at least one chemical illuminating reagent and sample injection on the chemical illuminating reagent and sample implanted layer (1)
Microchannel (5), the chemical illuminating reagent and sample injection microchannel (5) be located at both sides cell culture fluid silicagel column (6) it
Between, the chemical illuminating reagent and sample injection microchannel (5) have with cell culture microchannel (4) and intersect connectivity points, describedization
The both ends for learning upside chemical illuminating reagent and the sample injection microchannel (5) of luminescence reagent and sample implanted layer (1) are set respectively
There are chemical illuminating reagent and sample injection silicagel column (7), be provided on the chemical illuminating reagent and sample injection silicagel column (7)
With chemical illuminating reagent and the solution injection hole of sample injection microchannel (5) unicom.
2. the micro-fluidic chemical luminous chip of cell tests is used for according to claim 1, it is characterised in that：The chemistry hair
Light reagent and sample implanted layer (1), cell culture layer (2) are made of polymer material.
3. the micro-fluidic chemical luminous chip according to claim 1 or claim 2 for cell tests, it is characterised in that：It is described thin
Born of the same parents cultivate microchannel (4) as 1-16 items, and the spacing between every two cell culture microchannels (4) is 1-10mm.
4. the micro-fluidic chemical luminous chip of cell tests is used for according to claim 3, it is characterised in that：The cell training
Support 50~150 μm of the depth of microchannel (4), 250~350 μm wide, long 15~20mm.
5. the micro-fluidic chemical luminous chip of cell tests is used for according to claim 3, it is characterised in that：The chemistry hair
Light reagent and sample injection microchannel (5) are that 1-24 items, every chemical illuminating reagent and sample injection microchannel (5) and every are thin
Born of the same parents, which cultivate microchannel (4), intersection connectivity points.
6. the micro-fluidic chemical luminous chip of cell tests is used for according to claim 5, it is characterised in that：The chemistry hair
Light reagent and sample injection microchannel (5) are 50~150 μm deep, 250~350 μm wide, long 25~40mm, every two chemiluminescences examination
Spacing between agent and sample injection microchannel (5) is 1~10mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721088476.0U CN207401491U (en) | 2017-08-28 | 2017-08-28 | For the micro-fluidic chemical luminous chip of cell tests |
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| CN201721088476.0U CN207401491U (en) | 2017-08-28 | 2017-08-28 | For the micro-fluidic chemical luminous chip of cell tests |
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| CN207401491U true CN207401491U (en) | 2018-05-25 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758995A (en) * | 2019-03-05 | 2019-05-17 | 大连理工大学 | A kind of Universal fluorescence fluid photochemistry microreactor part and its 3D printing manufacturing method |
| CN110684656A (en) * | 2019-06-29 | 2020-01-14 | 东南大学 | Integrated micro-fluidic chip platform based on SERS technology |
| WO2021147988A1 (en) * | 2020-01-22 | 2021-07-29 | 京东方科技集团股份有限公司 | Biochip and manufacturing method therefor |
| US11872556B2 (en) | 2019-03-05 | 2024-01-16 | Dalian University Of Technology | General-purpose fluorescent fluid photochemical microreactor and manufacturing method therefor by 3D printing |
-
2017
- 2017-08-28 CN CN201721088476.0U patent/CN207401491U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109758995A (en) * | 2019-03-05 | 2019-05-17 | 大连理工大学 | A kind of Universal fluorescence fluid photochemistry microreactor part and its 3D printing manufacturing method |
| US11872556B2 (en) | 2019-03-05 | 2024-01-16 | Dalian University Of Technology | General-purpose fluorescent fluid photochemical microreactor and manufacturing method therefor by 3D printing |
| CN110684656A (en) * | 2019-06-29 | 2020-01-14 | 东南大学 | Integrated micro-fluidic chip platform based on SERS technology |
| WO2021147988A1 (en) * | 2020-01-22 | 2021-07-29 | 京东方科技集团股份有限公司 | Biochip and manufacturing method therefor |
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