CN204779592U - Three gas incubators of air current switching -over formula - Google Patents

Three gas incubators of air current switching -over formula Download PDF

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Publication number
CN204779592U
CN204779592U CN201520452969.2U CN201520452969U CN204779592U CN 204779592 U CN204779592 U CN 204779592U CN 201520452969 U CN201520452969 U CN 201520452969U CN 204779592 U CN204779592 U CN 204779592U
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gas
incubator
incubator body
induction
dividing plate
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CN201520452969.2U
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陈蕾
祁少海
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First Affiliated Hospital of Sun Yat Sen University
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First Affiliated Hospital of Sun Yat Sen University
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Abstract

The utility model discloses a three gas incubators of air current switching -over formula, mix gas tank, a plurality of incubator body including one, this internal at least one baffle that is equipped with of every incubator, each baffle is parallel with the diapire of incubator body, and the baffle is separated the incubator body and is formed multilayer inclosed relatively and train the room, and train the room correspondence for every layer and dispose the induction -pipe, the one end of induction -pipe and the mist export intercommunication that mixes the gas tank, the other end one -to -one reposition of redundant personnel intercommunication of induction -pipe is each layer cultivation room extremely. The utility model discloses can commutate to the mist of output, this is internal to get into each different incubator to make this internal oxygen concentration everywhere of incubator unified, be beneficial to and train in batches.

Description

Airflow-reversing formula three gas incubator
Technical field
The utility model relates to incubator, particularly relates to airflow-reversing formula three gas incubator.
Background technology
At present, the incubator of cell, microorganism etc. often need possess persistence hyperoxia or hypoxemia cultivation function.Three traditional gas incubators are by the nitrogen in gas cylinder through air pump, carbon dioxide gas and air (if culture condition is hyperoxia) are sent in incubator body along pore and are entered mixing, although can the more cell of disposable placement in this large-scale incubator body, the cultures such as microorganism, but because incubator internal capacity is bigger than normal, the flexible of culture volume cannot be carried out according to concrete cultivation amount and situation, after cultivating door opening or closing, internal gas governing speed is slow, air consumption large (conventional 67L incubator can exhaust one bottle in 10 days), cause experimental gas waste outside experiment condition also can be caused strictly not control, cell or microorganism long time-histories easily have crossed contamination and pollute uppity problem when cultivating.
Utility model content
In order to overcome the deficiencies in the prior art, the purpose of this utility model is to provide airflow-reversing formula hypoxemia incubator, the low-oxygen gas mixture body exported can be commutated, enter in each different incubator body, make incubator body relatively independent but the unification of internal oxygen concentration, be beneficial to Batch Culture.
The purpose of this utility model realizes by the following technical solutions:
Airflow-reversing formula three gas incubator, comprise a gas mixed box, multiple incubator body, at least one dividing plate is provided with in each incubator body, each dividing plate is parallel with the diapire of incubator body, incubator body is separated to form relatively airtight multilayer culturing room by dividing plate, Mei Ceng culturing room correspondence is configured with induction pipe, and one end of induction pipe is communicated with the mixed gas outlet of gas mixed box, and the other end one_to_one corresponding shunting of induction pipe is communicated to Ge Ceng culturing room.
Preferably, described gas mixed box is provided with the first air inlet port for passing into extraneous air, for pass into outside carbonic acid gas the second air inlet port, for passing into the 3rd air inlet port of external inert gas.
Preferably, multiple oxygen concn induction regulator is provided with in described gas mixed box.
Preferably, described dividing plate has breather hole.
Compared to existing technology, the beneficial effects of the utility model are:
The utility model makes full use of the original structure of three gas incubators and function, more economically.By structure and the capacity advantage of gas mixed box and incubator body, the easy gas that can input the output of small volume economically mixes and sensitive regulation and control, gas and vapor permeation and regulation and control efficiency higher.Mixed gas is commutated by induction pipe; enter in each different incubator body; each incubator body is relatively independent while the unified oxygen concentration of maintenance; the propagation of polluting when avoiding cultivation while the unity ensureing experiment condition, can play better protecting effect for batch, long-time cell cultures.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model airflow-reversing formula three gas incubator;
Fig. 2 is the structural representation of the utility model gas mixed box.
In figure: 1, gas mixed box; 11, the first air inlet port; 12, the second air inlet port; 13, the 3rd air inlet port; 2, incubator body; 3, dividing plate; 31, breather hole; 4, culturing room; 5, induction pipe; 6, oxygen concn induction regulator oxygen concn induction regulator; 7, backboard; 8, extraneous gas take-off equipment.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the utility model is described further:
Airflow-reversing formula three gas incubator as shown in Fig. 1 ~ 2, comprise a gas mixed box 1, multiple incubator body 2, at least one dividing plate 3 is provided with in each incubator body 2, each dividing plate 3 is parallel with the diapire of incubator body 2, incubator body 2 is separated to form relatively airtight multilayer culturing room 4 by dividing plate 3, Mei Ceng culturing room 4 correspondence is configured with induction pipe 5, and one end of induction pipe 5 is communicated with the mixed gas outlet of gas mixed box 1, and the other end one_to_one corresponding shunting of induction pipe 5 is communicated to Ge Ceng culturing room 4.Wherein, each incubator body defines parallel form by the connection of induction pipe 5.This example is specially, gas mixed box 1 is without backboard, in covering in and covering on the backboard 7 of original three gas incubators, by the original air inlet port of former three gas incubator and oxygen concn induction regulator 6, accordingly, by extraneous gas take-off equipments 8 such as oxygen cylinders, to input oxygen in gas mixed box 1, to export low-oxygen gas mixture body.Establish water pond position (lowest layer) and two dividing plates 3 in each incubator body 2, each dividing plate 3 is parallel with the bottom surface of incubator body 2, can determine whether extract according to culture dish/plate height and manipulation require.The gas of culturing cell is entered in gas mixed box 1 by original path, and flows in each incubator body 2 through the connected induction pipe of gas mixed box 15 points.After incubator body 2 is closed, each incubator body 2 forms relatively airtight multilayer culturing room.
Each incubator body 2 internal gas when chamber door is closed keeps steady state, and in the case that chamber door causes after opening, oxygen concn change will activate oxygen concn induction regulator 6 in gas mixed box 1, readjusts by inputting gas and to stablize in case oxygen concn in setting level.
By dividing plate 3, incubator body 2 is separated to form multiple culturing room 4, more culture can be held with this, simultaneously, under the effect of induction pipe 5, low-oxygen gas mixture body (or the high-oxygen gas mixture body) shunting that commutates exported by gas mixed box 1 passes into each culturing room 4, thus make each culture in each culturing room 4 obtain being tending towards uniform oxygen concentration, to ensure the accurate realization of culture condition, the concrete oxygen concentration of different heights in incubator body 2 that also makes is unified, is beneficial to Batch Culture.Wherein, induction pipe 5 can arrange threeway or multiport valve, be connected to multiple segmentation pipe fitting to make a pipe fitting shunt and then make oxygen mixed gas be communicated to each culturing room 4.Utilize the air inlet port on the backboard 7 of former three gas incubators and oxygen concn induction regulator 6, first air, carbonic acid gas and rare gas element will enter gas mixed box 1 carries out mixing and regulating, the mixed gas of the different oxygen concentrations formed, and will be sent by each induction pipe 5 through it.Incubator body 2 can be made into the casing with switch gate, picks and places culture to facilitate.
When incubator body 2 chamber door is closed, internal gas oxygen concn will be undertaken finely tuning by oxygen concn induction regulator on backboard 76 and keep stable, when opening chamber door, in incubator body 2 casees, the original gaseous equilibrium state of gas is broken, and on backboard 7, all kinds of for startup gas enters and carries out regulating until rebuild balance by oxygen concn induction regulator 6 once again.
For ease of external device (ED), air, carbonic acid gas and rare gas element are passed into gas mixed box 1 respectively to mix, gas mixed box 1 is provided with the first air inlet port 11 passing into extraneous air, for pass into outside carbonic acid gas the second air inlet port 12, for passing into the 3rd air inlet port 13 of external inert gas.。
For detecting the oxygen concentration in gas mixed box 1, the oxygen obtaining desired concn with this is to carry out culture cultivation, be provided with multiple oxygen concn induction regulator 6 in gas mixed box 1, the data that this oxygen concn induction regulator 6 obtains control being used for the amount of oxygen that external device (ED) passes into gas mixed box 1.
For ensureing that in incubator body 2, oxygen concentration is balanced, dividing plate 3 has breather hole 31 further everywhere.
To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the utility model claim.

Claims (4)

1. airflow-reversing formula three gas incubator, it is characterized in that: comprise a gas mixed box, multiple incubator body, at least one dividing plate is provided with in each incubator body, each dividing plate is parallel with the diapire of incubator body, incubator body is separated to form relatively airtight multilayer culturing room by dividing plate, Mei Ceng culturing room correspondence is configured with induction pipe, and one end of induction pipe is communicated with the mixed gas outlet of gas mixed box, and the other end one_to_one corresponding shunting of induction pipe is communicated to Ge Ceng culturing room.
2. airflow-reversing formula three gas incubator according to claim 1, it is characterized in that: described gas mixed box is provided with the first air inlet port for passing into extraneous air, for pass into outside carbonic acid gas the second air inlet port, for passing into the 3rd air inlet port of external inert gas.
3. airflow-reversing formula three gas incubator according to claim 1, is characterized in that: be provided with multiple oxygen concn induction regulator in described gas mixed box.
4. the airflow-reversing formula three gas incubator according to any one of claims 1 to 3, is characterized in that: described dividing plate has breather hole.
CN201520452969.2U 2015-06-26 2015-06-26 Three gas incubators of air current switching -over formula Active CN204779592U (en)

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CN201520452969.2U CN204779592U (en) 2015-06-26 2015-06-26 Three gas incubators of air current switching -over formula

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Application Number Priority Date Filing Date Title
CN201520452969.2U CN204779592U (en) 2015-06-26 2015-06-26 Three gas incubators of air current switching -over formula

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669667A (en) * 2019-09-27 2020-01-10 深圳先进技术研究院 Oxygen concentration controllable multi-channel culture system

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669667A (en) * 2019-09-27 2020-01-10 深圳先进技术研究院 Oxygen concentration controllable multi-channel culture system

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