CN204679511U - A kind of mutton rapid detection card - Google Patents
A kind of mutton rapid detection card Download PDFInfo
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- CN204679511U CN204679511U CN201520394950.7U CN201520394950U CN204679511U CN 204679511 U CN204679511 U CN 204679511U CN 201520394950 U CN201520394950 U CN 201520394950U CN 204679511 U CN204679511 U CN 204679511U
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- mutton
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Abstract
A kind of mutton rapid detection card of the utility model, belongs to meat detection technique field.There is test-strips in test card shell of the present utility model, formed by supporting backboard pastes sample pad, collaurum (or latex particle) film, nitrocellulose filter and absorbing membrane successively; Collaurum (or latex particle) film is the glass fibre membrane containing anti-sheep haemoglobin monoclonal antibody collaurum (or latex particle) label, nitrocellulose filter transversely has two detection zone, article one, be the detection zone containing anti-sheep haemoglobin monoclonal antibody, another is the quality control band containing anti-mouse antibody.Cleaning Principle of the present utility model is that the mutton haemoglobin of the method direct-detection meat sample of applied immunology is to judge that mutton is true and false; The utility model mutton rapid detection card is easy to preparation, and fast easy to use, result is accurate.
Description
Technical field
The utility model relates to a kind of mutton rapid detection card, is a kind of detection utensil of quick detection mutton, belongs to meat detection technique field.
Background technology
Mutton is nutritious, delicious meat, is one of meat that the whole world is general.Popular especially in China, become common people's mutton meat similar to beef, but meat flavour is denseer.Mutton wants delicate compared with the meat of pork, few compared with the fat of pork and beef, cholesterol level.It can drive chill, health can be mended again, general cough due to wind-cold evil, chronic bronchitis, cold of insufficiency type asthma, deficiency of the kidney impotence, belly crymodynia, body void are afraid of cold, soreness and weakness of waist and knees, lean and haggard, qi-blood deficiency, after being ill or postpartum health void all empty shapes such as to lose and all have treatment and help effect, be most appropriate to eat winter, therefore be called as winter tonic, deeply popular to people.
Due to mutton extensive market, mutton forgery case happens occasionally.As the saying goes " hanging out a sheep's head but actually sell dog's meat ", now hanging sheepshead, to sell " mutton fraud " problems such as duck, horseflesh, pork, fox meat, rat meat, chicken of common occurrence, the mutton bought in hand is any meat on earth, believes that many people can produce this query, but from can't see again on the surface.Be all adopt genetic test at present, require high to experimental instrument and equipment, method complicated operation, require high to the professional knowledge of operating personnel, can only carry out in Specialty Experiment indoor, waste time and energy.Therefore research and develop a kind of detection utensil detecting again mutton accurately and reliably to be quickly and easily very important.
Openly not needing specific installation to carry out the method for meat qualification although also have, is all based on technique of gene detection, such as:
Documents CN102534035A discloses a kind of kit of quick discriminating beef and mutton, kit is made up of jointly 1 group of primer with high specific, 2 × isothermal reaction damping fluid, BstDNA polymerase and dyestuff, is to utilize LAMP technology and animal mitochondria DNA to combine the method identified beef and mutton.
Documents CN102337328A discloses a kind of method differentiating pig, beef or mutton and goods thereof, by round pcr and gel electrophoresis technology, utilize a pair detection primer to differentiate the mitochondrial DNA of fresh pig, cattle and sheep, thus pig, ox, goat, sheep are made a distinction.
Due to, must a certain amount of sheep blood be contained in mutton, and erythrocytic 90% in sheep blood is made up of haemoglobin.Generally, haemoglobin (Hb) is combined into by globin and heme, and the haemoglobin of often kind of animal has homology, but also there are differences, and therefore the haemoglobin of often kind of animal has the antigenicity of some uniquenesses.Based on the antigenic specificity of the haemoglobin in the animal blood of separate sources, special immunological detection method can be set up and carry out detecting and identifying.According to the difference of haemoglobin in meat, the differentiation of meat can be carried out.
The utility model is intended providing a kind of detection utensil based on sheep hemoglobin immune, immunology is taked to compete ratio juris, a kind of quick detection reagent of association colloid gold (or latex particle) labelling technique and immunochromatographic method design, the sheep haemoglobin in meat sample can be detected fast specifically, thus judge meat sample whether as mutton.Develop the color according to the presence or absence of test card sample detection line (T) and nature controlling line (C) and to judge in sample whether as mutton.Reaction is a kind of detection method utilizing sheep haemoglobin and set up, and has the special advantage such as fast.Test card is fast simple to operate simultaneously, without the need to special instrument and equipment, is applicable to execute-in-place.Due to sample size need less, fast easy, be applicable to the examination of great amount of samples.Result is accurate, and highly sensitive, accuracy rate is large.
Utility model content
The purpose of this utility model is to provide a kind of detection utensil that can detect mutton fast, whether direct applied immunology method is carried out detecting and determined in meat sample is mutton, overcome the shortcomings such as the complex operation of existing genetic test, set up the detection method based on immunological specificity.
For solving technical matters of the present utility model, the technical scheme adopted is as follows:
The test card shell that a kind of mutton rapid detection card is included in rectangular flat shell shape and the test-strips be arranged in shell; Described test card case surface has detection fenestra and well; Described test-strips is the fillet thin slice of sandwich construction, formed by supporting backboard pastes sample pad, colloidal gold film, nitrocellulose filter and absorbing membrane thereon successively by adhesive sticker; Stacked stickup nitrocellulose filter in the middle part of described supporting backboard, the stacked stickup absorbing membrane in described supporting backboard one end, the stacked stickup sample pad of the other end, inner and the described sample pad the inner of described absorbing membrane overlaps with nitrocellulose filter separately respectively, at the clinch of described sample pad and nitrocellulose filter, sandwiched stickup one section of colloidal gold film between the two; Described colloidal gold film is the glass fibre membrane containing anti-sheep hemoglobin antibodies colloid gold label; Described nitrocellulose filter is provided with successively a detection zone and a quality control band, described detection zone is the detection zone containing anti-sheep haemoglobin monoclonal antibody; Described quality control band is the quality control band containing anti-mouse antibody; Described test-strips is inserted in test card shell, and described sample pad is just to well, and described nitrocellulose filter is just to detection fenestra.
Described colloidal gold film also can be latex particle film, and described latex particle film is the glass fibre membrane containing anti-sheep hemoglobin antibodies latex particle mark.
Preferably, described test card shell is made up of cap and shell block two semi join, and described detection fenestra and well are opened on described test card shell cap.
Preferably, the test card shell of described rectangular flat shell shape is by long 70mm, and wide 20mm, thick 5mm, the engineering plastics of shell wall thickness 1mm are made.
Preferably, described test-strips (2) length of a film 60mm, the wide 4mm of sheet, thickness 2.5mm.
Preferably, described supporting backboard (21) is polyvinyl chloride plastic sheets, thick about 0.5mm, long 60mm, wide 4mm.
Preferably, described sample pad (22) is water adsorption glass tunica fibrosa, thick 0.5-0.8mm, long 20mm, wide 4mm.
Preferably, described colloidal gold film (23) thick 0.5-0.8mm, long 5mm, wide 4mm.
Preferably, described nitrocellulose filter (24) thick 0.5mm, long 20-25mm, wide 4mm; Described detection zone (241) and quality control band (242) are in the upper spaced arrangement of described nitrocellulose filter (24).
Preferably, described absorbing membrane (25) is thieving paper, thick 0.5-0.8mm, long 16-20mm, wide 4mm.
Preferably, described colloidal gold film (23), sample pad (22) and absorbing membrane (25) are 1-2mm with the lap of splice of described nitrocellulose filter (24).
Adopt above-mentioned mutton rapid detection card to detect the detection method of mutton fast, be the content of sheep haemoglobin in the method direct-detection meat sample of applied immunology, concrete disposal route is as follows:
1. extract sample liquid: the meat sample taking 1.0g chopping, is placed in the 5ml reaction tube containing 3ml reactant liquor, mixes, react 5 minutes under room temperature, obtain sample liquid to be detected, detect for mutton;
The formula of described reactant liquor is: NH
4cl 8.29g, NaHCO
31.00g, Na
2eDTA 37.2mg, distilled water 1000ml;
2. test card detects: first sample liquid to be detected be instilled in the sample pad of described mutton rapid detection card from described well, due to syphonic effect principle, drive anti-sheep hemoglobin antibodies colloid gold label thing contained by sample liquid to be detected and colloidal gold film together to described cellulose nitrate membrane diffusion, observations in 5-10 minute.
3. testing result contrast: the key reaction of test card is immunologic antigen and antibody response, if when meat sample to be checked is mutton, add sample liquid in described well after, namely with in described colloidal gold film sheep haemoglobin in sample liquid reacts with the anti-sheep hemoglobin antibodies of colloid gold label, and toward described nitrocellulose filter migration, in transition process, again with the anti-sheep haemoglobin monoclonal antibody reactive that contains in described detection zone, form the sandwich complex of antibody-antigen-antibody, thus described detection zone shows brownish red; Meanwhile, the anti-sheep hemoglobin antibodies of colloid gold label that is that dissociate or that combine moves to described quality control band and reacts with anti-mouse antibody wherein, forms brownish red band, thus colour developing; The result of key reaction has following 3 kinds of situations:
A, manifest brownish red trace band when described detection zone and described quality control band simultaneously, represent that testing result be positive, illustrate that being detected sample is mutton;
B, when described detection zone not Show Color, only have described quality control band to manifest brownish red trace, testing result is negative, illustrates that being detected sample is not mutton;
C, when described detection zone and described quality control band all do not develop the color, then show inspection test-strips lost efficacy.
The beneficial effects of the utility model: the utility model can detect the sheep haemoglobin in meat sample fast, specifically, thus judge meat sample whether as mutton; The utility model is easy to preparation and saves testing cost, easy to use, and detect fast, highly sensitive, result is accurate.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the utility model embodiment, be briefly described to the accompanying drawing used required in embodiment below, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the utility model mutton rapid detection card shell and test-strips arrangement schematic diagram in the enclosure;
Fig. 2 is the test-strips structural representation of mutton rapid detection card of the present utility model;
Fig. 3 be the test-strips of mutton rapid detection card of the present utility model nitrocellulose filter on show trace schematic diagram;
Accompanying drawing mark illustrates:
1, shell; 11, fenestra is detected; 12, well;
2, test-strips; 21, backboard is supported; 22, sample pad; 23, colloidal gold film (or latex particle film); 24, nitrocellulose filter; 25, absorbing membrane; 241, detection zone; 242, quality control band.
Embodiment
With below by reference to the accompanying drawings and specific embodiment the utility model is further described:
Test card embodiment 1
A kind of mutton rapid detection card, as shown in Figure 1, it arranges test-strips 2 in test card shell 1, and test card shell 1 is rectangular flat shell shape shell, and long 70mm, wide 20mm, thick 5mm, shell wall thickness 1mm, is made up of engineering plastics.Test card shell 1 is made up of cap and shell block two semi join, test card shell 1 cap has and detects fenestra 11 and well 12.Test-strips 2 is placed in the shell block of test card shell 1.
As shown in Figure 2, test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, and the wide about 4mm of sheet, thickness is about 2.5mm.Test-strips 2 is sandwich construction, and bottom is supporting backboard 21, is polyvinyl chloride plastic sheets, thick about 0.5mm, long 60mm, wide 4mm; Stacked stickup nitrocellulose filter 24 in the middle part of supporting backboard 21, the thick 0.5mm of nitrocellulose filter 24, long 20-25mm, wide 4mm.As shown in Figure 3, nitrocellulose filter 24 has two isolated laterally display trace bands, in two display trace bands, one is detection zone 241, containing anti-sheep hemoglobin antibodies; Another is quality control band 242, containing anti-mouse antibody.Stacked stickup absorbing membrane 25 on supporting backboard 21 1 end, stacked stickup sample pad 22 on supporting backboard 21 the other end, absorbing membrane 25 is inner to be overlapped with nitrocellulose filter 24 one end separately respectively with sample pad 22 the inner, at the clinch of sample pad 22 with nitrocellulose filter 24, sandwiched stickup one section of colloidal gold film 23 between the two, colloidal gold film 23 is the glass fibre membranes containing anti-sheep hemoglobin antibodies colloid gold label thing.The thick 0.5-0.8mm of colloidal gold film 23, long 5mm, wide 4mm.The thick 0.5-0.8mm of sample pad 22, long 20mm, wide 4mm.The thick 0.5-0.8mm of absorbing membrane 25, long 16-20mm, wide 4mm.Colloidal gold film 23, sample pad 22 and absorbing membrane 25 and nitrocellulose filter 24 lap of splice are 1-2mm.Absorbing membrane 25 material is thieving paper, and sample pad 22 material is water adsorption glass tunica fibrosa.
The utility model mutton test card is used for the sheep haemoglobin detected specifically fast in meat sample, thus judges meat sample whether as mutton.
Test card embodiment 2
The difference of the present embodiment and test card embodiment 1 is, colloidal gold film 23 is latex particle film, and latex particle film is the glass fibre membrane containing anti-sheep hemoglobin antibodies latex particle mark.
Detection method embodiment 1
The detection method of the present embodiment is the content of sheep haemoglobin in the method direct-detection meat sample of applied immunology, and concrete disposal route is as follows:
1. extract sample liquid: the meat sample taking 1.0g chopping, is placed in the 5ml reaction tube containing 3ml reactant liquor, mixes, react 5 minutes under room temperature, obtain sample liquid to be detected, detect for mutton;
The formula of described reactant liquor is: NH
4cl 8.29g, NaHCO
31.00g, Na
2eDTA 37.2mg, distilled water 1000ml;
2. test card detects: first sample liquid to be detected be instilled into from described well 12 in the sample pad 22 of described mutton rapid detection card, due to syphonic effect principle, the anti-sheep hemoglobin antibodies colloid gold label thing contained by sample liquid to be detected and colloidal gold film 23 is driven to spread to described nitrocellulose filter 24 together, observations in 5-10 minute.
3. testing result contrast: the key reaction of test card is immunologic antigen and antibody response, if when meat sample to be checked is mutton, add sample liquid in described well 12 after, namely sheep haemoglobin in sample liquid reacts with the anti-sheep hemoglobin antibodies of the interior colloid gold label of described colloidal gold film 23, and move toward described nitrocellulose filter 24, in transition process, again with the anti-sheep haemoglobin monoclonal antibody reactive that contains in described detection zone 241, form the sandwich complex of antibody-antigen-antibody, thus described detection zone 241 shows brownish red; Meanwhile, the anti-sheep hemoglobin antibodies of colloid gold label that is that dissociate or that combine moves to described quality control band 242 and reacts with anti-mouse antibody wherein, forms brownish red band, thus colour developing; The result of key reaction has following 3 kinds of situations:
A, manifest brownish red trace band when described detection zone 241 and described quality control band 242 simultaneously, represent that testing result be positive, illustrate that being detected sample is mutton;
B, when described detection zone 241 not Show Color, only have described quality control band 242 to manifest brownish red trace, testing result is negative, illustrates that being detected sample is not mutton;
C, when described detection zone 241 and described quality control band 242 all do not develop the color, then show that test-strips 2 lost efficacy.
The utility model can detect the sheep haemoglobin in meat sample fast, specifically, thus judges meat sample whether as mutton; The utility model is easy to preparation and saves testing cost, easy to use, and detect fast, highly sensitive, result is accurate.
Detection method embodiment 2
The method detected is carried out by the mutton rapid detection card described in test card embodiment 2, specific as follows:
1. extract sample liquid: the meat sample taking 1.0g chopping, is placed in the 5ml reaction tube containing 3ml reactant liquor, mixes, react 5 minutes under room temperature, obtain sample liquid to be detected, detect for mutton;
The formula of described reactant liquor is: NH
4cl 8.29g, NaHCO
31.00g, Na
2eDTA 37.2mg, distilled water 1000ml;
2. test card detects: first sample liquid to be detected be instilled into from described well 12 in the sample pad 22 of described mutton rapid detection card, due to syphonic effect principle, the anti-sheep hemoglobin antibodies latex particle label contained by sample liquid to be detected and latex particle film 23 is driven to spread to described nitrocellulose filter 24 together, observations in 5-10 minute.
3. testing result contrast: the key reaction of test card is immunologic antigen and antibody response, if when meat sample to be checked is mutton, add sample liquid in described well 12 after, namely with in described latex particle film 23 sheep haemoglobin in sample liquid reacts with the anti-sheep hemoglobin antibodies that latex particle marks, and move toward described nitrocellulose filter 24, in transition process, again with the anti-sheep haemoglobin monoclonal antibody reactive that contains in described detection zone 241, form the sandwich complex of antibody-antigen-antibody, thus described detection zone 241 shows brownish red, meanwhile, the anti-sheep hemoglobin antibodies of colloid gold label that is that dissociate or that combine moves to described quality control band 242 and reacts with anti-mouse antibody wherein, forms brownish red band, thus colour developing, the result of key reaction has following 3 kinds of situations:
A, manifest brownish red trace band when described detection zone 241 and described quality control band 242 simultaneously, represent that testing result be positive, illustrate that being detected sample is mutton;
B, when described detection zone 241 not Show Color, only have described quality control band 242 to manifest brownish red trace, testing result is negative, illustrates that being detected sample is not mutton;
C, when described detection zone 241 and described quality control band 242 all do not develop the color, then show that test-strips 2 lost efficacy.
Application Example
Test by the principle described in detection method embodiment 1, detection mutton, pork, beef sample is carried out by the test card described in the utility model test card embodiment 1, repeat 10 times, the testing result that wherein only meat samples detects is detection line and the equal outlet of nature controlling line on cellulose membrane, the testing result of the sample detection of pork and beef is only nature controlling line outlet on cellulose membrane, and testing result is accurate.Repeat to detect the colour developing degree of depth for 10 times homogeneous, testing result display is consistent.
Stability test: test card is put into aluminium foil bag and packs, simultaneously each packaging of aluminium foil bag in-built is responsible for a task until it is completed drying prescription, room temperature preservation after 12 months indices all meet.
Above-described embodiment further illustrates of the present utility model, instead of limit scope of the present utility model.Do not depart from whole technical scope of the present utility model, can various amendment and change be carried out.
Claims (4)
1. a mutton rapid detection card, is characterized in that: in the test card shell (1) of rectangular flat shell shape, arrange test-strips (2);
There are detection fenestra (11) and well (12) in described test card shell (1) surface;
Described test-strips (2) is the fillet thin slice of sandwich construction, formed by supporting backboard (21) pastes sample pad (22), colloidal gold film (23), nitrocellulose filter (24) and absorbing membrane (25) thereon successively by adhesive sticker; The stacked stickup nitrocellulose filter (24) in described supporting backboard (21) middle part, the stacked stickup absorbing membrane (25) in described supporting backboard (21) one end, the stacked stickup sample pad (22) of the other end, described absorbing membrane (25) is inner to be overlapped with nitrocellulose filter (24) separately respectively with described sample pad (22) the inner, at the clinch of described sample pad (22) with nitrocellulose filter (24), the one section of colloidal gold film (23) of sandwiched stickup between the two;
Described colloidal gold film (23) is the glass fibre membrane containing anti-sheep hemoglobin antibodies colloid gold label;
Described nitrocellulose filter (24) is provided with successively a detection zone (241) and a quality control band (242), described detection zone (241) is the detection zone containing anti-sheep haemoglobin monoclonal antibody; Described quality control band (242) is the quality control band containing anti-mouse antibody;
Described test-strips (2) is inserted in test card shell (1), and described sample pad (22) is just to well (12), and described nitrocellulose filter (24) is just to detection fenestra (11).
2. a kind of mutton rapid detection card according to claim 1, is characterized in that: described colloidal gold film (23) is latex particle film, and described latex particle film is the glass fibre membrane containing anti-sheep hemoglobin antibodies latex particle mark.
3. a kind of mutton rapid detection card according to claim 1 or 2 any one, is characterized in that,
Described test card shell (1) is made up of cap and shell block two semi join, and described detection fenestra (11) and well (12) are opened on described test card shell (1) cap;
The test card shell (1) of described rectangular flat shell shape is by long 70mm, and wide 20mm, thick 5mm, the engineering plastics of shell wall thickness 1mm are made.
4. a kind of mutton rapid detection card according to claim 1 or 2 any one, is characterized in that,
Described test-strips (2) length of a film 60mm, the wide 4mm of sheet, thickness 2.5mm;
Described supporting backboard (21) is polyvinyl chloride plastic sheets, thick about 0.5mm, long 60mm, wide 4mm;
Described sample pad (22) is water adsorption glass tunica fibrosa, thick 0.5-0.8mm, long 20mm, wide 4mm;
Described colloidal gold film (23) thick 0.5-0.8mm, long 5mm, wide 4mm;
Described nitrocellulose filter (24) thick 0.5mm, long 20-25mm, wide 4mm; Described detection zone (241) and quality control band (242) are in the upper spaced arrangement of described nitrocellulose filter (24);
Described absorbing membrane (25) is thieving paper, thick 0.5-0.8mm, long 16-20mm, wide 4mm;
Described colloidal gold film (23), sample pad (22) and absorbing membrane (25) are 1-2mm with the lap of splice of described nitrocellulose filter (24).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520394950.7U CN204679511U (en) | 2015-06-10 | 2015-06-10 | A kind of mutton rapid detection card |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520394950.7U CN204679511U (en) | 2015-06-10 | 2015-06-10 | A kind of mutton rapid detection card |
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| CN204679511U true CN204679511U (en) | 2015-09-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104914250A (en) * | 2015-06-10 | 2015-09-16 | 长沙安迪生物科技有限公司 | Rapid mutton detection card and detection method thereof |
-
2015
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104914250A (en) * | 2015-06-10 | 2015-09-16 | 长沙安迪生物科技有限公司 | Rapid mutton detection card and detection method thereof |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150930 Termination date: 20190610 |
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| CF01 | Termination of patent right due to non-payment of annual fee |