CN204608009U - A kind of Markers for Detection test kit of blueberry heat resistant variety screening - Google Patents
A kind of Markers for Detection test kit of blueberry heat resistant variety screening Download PDFInfo
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- CN204608009U CN204608009U CN201520299018.6U CN201520299018U CN204608009U CN 204608009 U CN204608009 U CN 204608009U CN 201520299018 U CN201520299018 U CN 201520299018U CN 204608009 U CN204608009 U CN 204608009U
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Abstract
The utility model discloses the Markers for Detection test kit of a kind of blueberry heat resistant variety screening, comprise box body and cover the lid on box body, box body comprises vacuum wall, attemperator, sponge permanent seat.Described sponge permanent seat is contained in attemperator, it is offered 3 large accommodating hole accommodating real-time quantitative PCR reaction reagent mixed solution Reagent Tubes and 3 little accommodating holes accommodating external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube respectively; The attemperator height of described box body is lower than vacuum wall, and the thermal insulation layer of described lid is protruding, and the end face that lid and box body contact is mutually chimeric to be mated; Described real-time quantitative PCR reaction reagent mixed solution Reagent Tube is stylostome, and be cylinder outside at the bottom of pipe, inner side is semisphere; Described external source object of reference Reagent Tube and described reference gene primer kit pipe and described FeSOD gene primer Reagent Tube shape different.The utility model uses simply, screening efficiency is high, good airproof performance.
Description
Technical field
The utility model relates to fruit tree molecular genetic breeding field, the Markers for Detection test kit of particularly a kind of blueberry heat resistant variety screening.
Background technology
Climate warming is global problem, and extreme climate is frequently occurred, causes various environment stress to growth and development of plants, as high temperature, low temperature, arid, saline and alkaline etc.High temperature makes harmful active oxygen in cell (ROS) produce and the running balance of removing is broken, and causes oxidative stress to plant.In plant materials, activating oxide accumulation increases, and affects the growth of plant, growth, even causes it dead, has a strong impact on crop product production and the quality of results.Therefore, the screening of heat resistant variety and cultivation, to plant health growth, improve crop yield extremely important.
Blueberry is rich in the mineral elements such as anthocyanin, flavonoid compound, multiple amino acids and phosphorus, iron, zinc, magnesium, has high economic worth and wide DEVELOPMENT PROSPECT, can be rated as " king of third generation in the world fruit ".In recent years, blueberry industry in the development of southern province rapidly.But because the Changjiang river areas to the south summer weather is scorching, sunshine is strong, easily causes high temperature injury to blueberry, even causes cessation of growth cessation, the heat-resisting blueberry kind therefore screening and cultivate applicable southern province cultivation has great importance to local production practice.
Utilize molecule marker and biotechnology deeply to excavate heat-stable material, the heat-resisting genetic improvement of blueberry can be accelerated.In oxygen scavenging activity system, a topmost approach is that SOD combines other antioxidases and carries out.SOD catalysis superoxide anion disproportionation is H
2o
2and O
2, in approach, remove the key enzyme of ROS for this reason.In plant materials, SOD can be divided into MnSOD(to be mainly positioned at plastosome according to its enzyme egg from the difference of metal prothetic group combined), Cu/ZnSOD(is mainly positioned at tenuigenin and chloroplast(id)) and FeSOD(be mainly positioned at chloroplast(id)).Wherein the ability such as FeSOD and disease resistance of plant, salt resistance, heat impedance, winter hardiness, salt tolerance is closely related.FeSOD expression amount can raise rapidly by these environment stress Effects of Factors, thus reduces plant oxidation stress damage.Visible FeSOD gene is resistance gene important in plant materials.Therefore, can blueberry Holstein Cattle be assisted to screen as molecule marker by FeSOD enzyme gene, greatly accelerate blueberry heat enduring vegetables breeding process.
Because current each reagent for detecting FeSOD gene is all that dispersion is contained in different test kits, when testing, after being placed on test-tube stand after the reagent of use need being taken out respectively respectively from the test kit that it holds, carry out follow-up test again, but because every day can test repeatedly, such every day will pick and place reagent many times back and forth, the frequent variations of temperature easily causes reagent inactivation, in addition, during current purchase reagent be all be contained in there is no heat insulation function carton inside, again it is put into refrigerator cold-storage rapidly after buying, it being taken out from refrigerator before test is placed on test-tube stand, again test-tube stand is placed on and is used for cold insulation on ice, but summer does experiment, ice easily melts, so many inconvenience can be caused to test, therefore propose a kind ofly required for test reagent to be positioned over same and can to become present problem demanding prompt solution in the test kit of cold insulation.
Utility model content
The utility model carries out to solve current Problems existing, and it is strong that object is to provide cold insulation that is highly sensitive, quick, easy-to-use, that have a good application prospect, and be convenient for carrying structure simple blueberry Holstein Cattle identification reagent box.To achieve these goals, the utility model have employed following technical scheme.
The Markers for Detection test kit of blueberry heat resistant variety screening, comprise box body and cover the lid on box body, described box body, comprise vacuum wall, attemperator and sponge permanent seat, described sponge permanent seat is contained in attemperator, it is offered 3 large accommodating holes and 3 little accommodating holes, described 3 large accommodating hole accommodating real-time quantitative PCR reaction reagent mixed solution Reagent Tubes, described 3 little accommodating holes accommodating external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube respectively; Described attemperator has bilayer structure, and interlayer is filled with cold-retaining agent, and outer field material is lagging material; Described vacuum wall is double-deck rigid plastics, and centre vacuumizes.
Described lid is identical with cell material, and skin is vacuum wall, and middle layer is the thermal insulation layer can filling cold-retaining agent, and internal layer is sponge pad.
Preferably, described 6 accommodating holes are point two row's arrangements on sponge permanent seat, upper row two large accommodating holes, lower row large accommodating hole and 3 little accommodating holes, 3 described little accommodating hole circular array, described large accommodating hole can hold 5mLEP pipe, and described little accommodating hole can hold 1.5mLEP pipe.
Preferably, described 6 accommodating hole degree of depth are less than sponge permanent seat height, and chamber bottom has sponge.
Preferably, the attemperator height of described box body is lower than vacuum wall, and the thermal insulation layer of described lid is convex, and the end face that lid and box body contact is mutually chimeric to be mated.
Preferably, described lid side is connected by rotating shaft with box body, and opposite side has a plastic hasp, and accordingly, this side of described box body is provided with corresponding plastic hasp locked part.
Preferably, described real-time quantitative PCR reaction reagent mixed solution Reagent Tube is the brown opaque EP pipe of 5mL, EP pipe shaft is provided with scale mark, scale mark place tube wall is white clear, scale mark is superfine, be cylinder outside at the bottom of EP pipe pipe, inner side is semisphere, and EP pipe pipe is stamped two kinds of unfolding mode of renovating out and outward winding; Described external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube are that 1.5mL EP manages, and pipe lid shape is respectively circular, oval, hexagon.
The utility model have employed vacuum wall and attemperator is in low temperature environment to keep real-time quantitative PCR reaction reagent mixed solution, external source object of reference, reference gene primer and FeSOD gene primer in transportation, prevents high temperature from causing reagent inactivation.Real-time quantitative PCR reaction reagent mixed solution Reagent Tube is that brown opaque reagent of avoiding is degraded, and pipe shaft indicates visible scale and is convenient to observe service condition, has two kinds of modes of uncapping, and is convenient to draw, and avoids too much exposing in atmosphere; External source object of reference, internal reference primer and FeSOD gene primer hold with the Reagent Tube of difform pipe lid, avoid obscuring in experimentation; The end face that lid and box body contact is mutually chimeric to be mated, and has plastic hasp, guaranteed reagent,G.R. box stopping property, convenient transport.The utility model can be fast and simple blueberry heat resistant variety is differentiated, use simple, highly sensitive, screening efficiency is high, the cycle is short, meanwhile, the reagent used has commercial supplier widely.
Accompanying drawing explanation
Fig. 1 is the utility model reagent cartridge configuration schematic diagram;
Fig. 2 is the structural representation of 5mLEP pipe.
Embodiment
As shown in Figure 1, the Markers for Detection test kit of the blueberry heat resistant variety screening that the utility model provides, comprises box body 1 and covers the lid 2 on box body 1.The side of lid 2 is connected by rotating shaft with box body 1, opposite side is provided with a plastic hasp 10, and accordingly, this side of box body 1 is provided with corresponding plastic hasp locked part 11, plastic hasp 10 can be buckled together with corresponding plastic hasp locked part 11, improves stopping property.The material of lid 2 is identical with box body 1 material, and its skin 21 is vacuum wall, and middle layer 22 is the thermal insulation layer can filling cold-retaining agent, and internal layer 23 is sponge pad.
The box body 1 of the present embodiment comprises vacuum wall 3, is arranged on the attemperator 4 inside vacuum wall 3 and is arranged on the sponge permanent seat 5 in the space of attemperator 4 formation.Vacuum wall 3 is double-deck rigid plastics, and centre vacuumizes, and internal and external environment is isolated.Attemperator 4 has bilayer structure, and interlayer is filled with cold-retaining agent, and outer field material is lagging material, better can ensure that in transportation, reagent is in low temperature environment.Attemperator 4 height of box body 1 is lower than vacuum wall 3, and the middle layer 22 of lid 2 is thermal insulation layer, and it is convex, and lid 2 and the end face that box body 1 contacts are mutually chimeric to be mated, and strengthens the stopping property of the present embodiment detection kit further.Sponge permanent seat 5 to be contained in attemperator 4 and to offer three large accommodating holes and three little accommodating holes 13, three large accommodating hole accommodating real-time quantitative PCR reaction reagent mixed solution Reagent Tubes, three little accommodating holes 13 accommodating external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube respectively.
In order to make, the detection kit of the present embodiment is more neat, saving space, six accommodating hole point two row's arrangements on sponge permanent seat, upper row two large accommodating holes, lower row large accommodating hole and three little accommodating holes 13, three little accommodating hole 13 circular array, large accommodating hole is the hole that can hold 5mL EP pipe, and little accommodating hole 13 is the hole of 1.5mL EP pipe; Six accommodating hole degree of depth are less than the height of sponge permanent seat 5, and chamber bottom has sponge, play shock absorption in transit, and container can be avoided to be shaken.
Due to a large amount of real-time quantitative PCR reaction reagent mixed solutions can be used in process of the test, but conveniently test kit is portable, in the present embodiment, real-time quantitative PCR reaction reagent mixed solution is divided in multiple Reagent Tube, again Reagent Tube is inserted in large accommodating hole, the quantity of large accommodating hole can be multiple, the quantity of the large accommodating hole of the present embodiment is three, and it holds real-time quantitative PCR reaction reagent mixed solution Reagent Tube 6 for holding three.The real-time quantitative PCR reaction reagent mixed solution Reagent Tube 6 of the present embodiment is the brown opaque EP pipe of 5mL, avoids the ingredient degradation to photo-labile in reagent; 5mL EP pipe shaft indicates scale mark 61, and scale mark 61 place tube wall is white clear, and scale mark is superfine, can indicator service condition; Be cylinder outside at the bottom of 5mL EP pipe pipe, inner side is semisphere, is convenient to place; 5mL EP pipe pipe lid 62 is renovated 621 and is opened two kinds of unfolding mode of outwarding winding with threaded cap 622, in actual mechanical process, can not unwinding thread lid 622, and only renovate 621 just can draw reagent by opening, avoid reagent too much to expose in atmosphere.
The external source object of reference Reagent Tube of the present embodiment, reference gene primer kit and FeSOD gene primer Reagent Tube are that 1.5mL EP manages, pipe lid shape is respectively circular, oval, hexagon, reagent of avoiding confusion causes the failure of an experiment, due to circle, oval and hexagonal shape is comparatively common, therefore not shown in accompanying drawing.
External source object of reference Reagent Tube is equipped with 50 ng/ μ L ' Dianas ' blueberry leaf cDNA, reference gene primer kit pipe is equipped with the GAPDH gene upstream and downstream primer of 10 μm of ol/L, and FeSOD gene primer Reagent Tube is equipped with the blueberry FeSOD gene upstream and downstream primer of 10 μm of ol/L.
The Markers for Detection test kit of the blueberry heat resistant variety screening using the utility model to provide, real-time quantitative PCR is carried out to the cDNA that the RNA reverse transcription of blueberry kind blade to be screened becomes, goes out to have the kind of good heat resistance shape according to blueberry kind FeSOD to be detected relative to the relative expression quantity examination of ' Diana '.Screening efficiency of the present utility model is better than existing method, convenient and swift.
Above embodiment only in order to the technical solution of the utility model to be described, but not is limited; Although be described in detail the utility model with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the utility model technical scheme required for protection.
Claims (6)
1. the Markers for Detection test kit of a blueberry heat resistant variety screening, comprise box body and cover the lid on box body, it is characterized in that, described box body, comprise vacuum wall, attemperator and sponge permanent seat, described sponge permanent seat is contained in attemperator, it is offered 3 large accommodating holes and 3 little accommodating holes, described 3 large accommodating hole accommodating real-time quantitative PCR reaction reagent mixed solution Reagent Tubes, described 3 little accommodating holes accommodating external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube respectively; Described attemperator has bilayer structure, and interlayer is filled with cold-retaining agent, and outer field material is lagging material; Described vacuum wall is double-deck rigid plastics, and centre vacuumizes;
Described lid is identical with cell material, and skin is vacuum wall, and middle layer is the thermal insulation layer can filling cold-retaining agent, and internal layer is sponge pad.
2. according to the Markers for Detection test kit of a kind of blueberry heat resistant variety screening described in claim 1, it is characterized in that, described 6 accommodating holes are point two row's arrangements on sponge permanent seat, upper row is two large accommodating holes, lower row is a large accommodating hole and 3 little accommodating holes, 3 described little accommodating hole circular array, described large accommodating hole can hold 5mLEP pipe, and described little accommodating hole can hold 1.5mLEP pipe.
3., according to the Markers for Detection test kit of a kind of blueberry heat resistant variety screening described in claim 2, it is characterized in that: described 6 accommodating hole degree of depth are less than sponge permanent seat height, and chamber bottom has sponge.
4. according to the Markers for Detection test kit of a kind of blueberry heat resistant variety screening described in claim 3, it is characterized in that, the attemperator height of described box body is lower than vacuum wall, and the thermal insulation layer of described lid is convex, and the end face that lid and box body contact is mutually chimeric to be mated.
5. according to the Markers for Detection test kit of a kind of blueberry heat resistant variety screening described in claim 4, it is characterized in that, described lid side is connected by rotating shaft with box body, and opposite side has a plastic hasp, accordingly, this side of described box body is provided with corresponding plastic hasp locked part.
6. according to the Markers for Detection test kit of a kind of blueberry heat resistant variety screening described in claim 5, it is characterized in that, described real-time quantitative PCR reaction reagent mixed solution Reagent Tube is the brown opaque EP pipe of 5mL, EP pipe shaft is provided with scale mark, scale mark place tube wall is white clear, be cylinder outside at the bottom of EP pipe pipe, inner side is semisphere, and EP pipe pipe is stamped two kinds of unfolding mode of renovating out and outward winding; Described external source object of reference Reagent Tube, reference gene primer kit pipe and FeSOD gene primer Reagent Tube are that 1.5mL EP manages, and pipe lid shape is respectively circular, oval, hexagon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520299018.6U CN204608009U (en) | 2015-05-11 | 2015-05-11 | A kind of Markers for Detection test kit of blueberry heat resistant variety screening |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520299018.6U CN204608009U (en) | 2015-05-11 | 2015-05-11 | A kind of Markers for Detection test kit of blueberry heat resistant variety screening |
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| Publication Number | Publication Date |
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| CN204608009U true CN204608009U (en) | 2015-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520299018.6U Expired - Fee Related CN204608009U (en) | 2015-05-11 | 2015-05-11 | A kind of Markers for Detection test kit of blueberry heat resistant variety screening |
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| CN (1) | CN204608009U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017101241A1 (en) * | 2015-12-17 | 2017-06-22 | 深圳精准医疗科技有限公司 | Box for lentivirus reagent kit |
-
2015
- 2015-05-11 CN CN201520299018.6U patent/CN204608009U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017101241A1 (en) * | 2015-12-17 | 2017-06-22 | 深圳精准医疗科技有限公司 | Box for lentivirus reagent kit |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150902 Termination date: 20160511 |
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| CF01 | Termination of patent right due to non-payment of annual fee |