CN204509338U - A kind of bacteria culture bottle measuring gas production rate - Google Patents

A kind of bacteria culture bottle measuring gas production rate Download PDF

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Publication number
CN204509338U
CN204509338U CN201520136214.1U CN201520136214U CN204509338U CN 204509338 U CN204509338 U CN 204509338U CN 201520136214 U CN201520136214 U CN 201520136214U CN 204509338 U CN204509338 U CN 204509338U
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Prior art keywords
airway
glass
production rate
bottle
glass airway
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Expired - Fee Related
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CN201520136214.1U
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Chinese (zh)
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李越
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Individual
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Individual
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Abstract

The utility model discloses a kind of bacteria culture bottle measuring gas production rate, comprise body, bottleneck, bottle cap, airway, wherein, body inwall is provided with vertical bar shaped small embossment, and the contact surface of small embossment and body inwall is arc; Body is provided with interlayer, and interlayer outer wall bottom is provided with water inlet, and top is provided with water outlet, and bottleneck is funnel-form; Bottle cap is connected with bottle sealing, and bottle cap is provided with air guide pore, is provided with airway in air guide pore; Airway comprises glass airway I, flexible pipe, glass airway II, and by hose connection between glass airway I and glass airway II, glass airway II free end is provided with scale.The utility model can carry out gas sampling, measures the generation of gas, and has wider useful range; Can also control the temperature of nutrient solution, structure is simple, good seal performance, and convenient cleaning, does not exist dead angle.There is a part of only replacing damage when damaging in any part of airway, cost-saving.

Description

A kind of bacteria culture bottle measuring gas production rate
Technical field
The utility model relates to a kind of culturing bottle, is specifically a kind of bacteria culture bottle measuring gas production rate.
Background technology
Some bacteriums can produce gas in culturing process, by the detection of gas production rate and quality can understand bacterium upgrowth situation and to nutrient solution fermentation decomposition situation.The method of current collection and confinement of gases has drainage and blind enema, drainage concrete operations are: prepare a tank, a bottle, fills water by bottle, filled with water in tank, bottle hangs and is buckled in the water of tank, and the tube mouth of Exhaust Gas is put in bottle bottom, both can fill gas to be collected in the drained rear bottle of the water in bottle, although this method is fairly simple, but operate cumbersome, and be difficult to when the amount of gas is fewer collect, and be not easy the amount calculating total gas.
Utility model content
In order to overcome above defect, the utility model provides a kind of bacteria culture bottle measuring gas production rate, and this culturing bottle structure is simple, good seal performance, convenient cleaning airway inwall, and there is a part of only replacing damage when damaging in any part of airway, cost-saving.
The purpose of this utility model is realized by following technical scheme:
A kind of bacteria culture bottle measuring gas production rate, comprise body, bottleneck, bottle cap, airway, it is characterized in that, described body inwall is provided with vertical bar shaped small embossment, the contact surface of small embossment and body inwall is arc, described body is provided with interlayer, and described interlayer outer wall top is provided with water outlet, and interlayer outer wall bottom is provided with water inlet; Described bottleneck is funnel-form; Described bottle cap is connected with bottle sealing, and bottle cap is provided with air guide pore, is provided with airway in air guide pore; Described airway comprises glass airway I, flexible pipe, glass airway II, by hose connection between glass airway I and glass airway II, glass airway I is inverted U structure, the free end of described glass airway I stretches into body inside through air guide pore, described glass airway I is tightly connected with air guide pore, described glass airway II is U-shaped structure, and the free end of glass airway II is provided with vertical scale.
During use, glass airway I and glass airway II are passed through hose connection, the free end of glass airway I is inserted in the air guide pore of bottle cap, airway is fixed, and inject appropriate amounts of sterilized water to the free end of glass airway II.Nutrient solution and bacterial classification are put into culturing bottle, build bottle cap and cultivable bacteria, the free end volume change of sterilized water at glass airway II can be read according to the vertical scale of glass airway II free end, the gas production rate of culturing bacterium can be calculated by sterilized water at the volume volume change of glass airway II free end.If culturing bottle needs heating, the heat-conducting liquid of certain temperature can be flowed into from water inlet and flow out heating culturing bottle from water outlet.
In order to realize the purpose of this utility model further, can also by the following technical solutions:
The top of described glass airway I is provided with vertical stopple coupon, is provided with sealing consent in the middle part of described stopple coupon, and sealing consent is provided with supporting sealing plug, is provided with ventilating pit in the middle part of sealing plug.The contact surface of described sealing consent and sealing plug is ground glass surface.Described glass airway II is fixed by support.
The beneficial effects of the utility model:
1, body is provided with interlayer is double-wall structure, sandwich structure can better keep the temperature of nutrient solution, the temperature of nutrient solution can also be controlled by the liquid adding certain temperature to water inlet, water inlet under, water outlet can better control upper the flow velocity adding liquid, makes the more uniform temperature of nutrient solution.Vertical bar shaped small embossment is conducive to stirring, and the contact surface of small embossment and culturing bottle inwall is arc, is conducive to the clean of culturing bottle, can not there is dead angle.Bottleneck is funnel-form, is conducive to lid bottle cap, also helps the sealing between bottle cap and bottleneck.By hose connection between glass airway I and glass airway II, facilitate the fixing of glass airway II, and glass airway II fixing after, also can not move the bottle cap of inhibiting belt glass airway I, the wider scale be provided with by the front of motor of glass airway II can read the size producing water displacement after gas, thus calculate the size of gas production rate, the upgrowth situation of bacterium and the nourishment of nutrient solution in culturing bottle can be known by the size of gas production rate; Airway is divided into three parts, convenient cleaning airway inwall, and a part of only replacing damage when damaging appears in any part, can be cost-saving.
2, stopple coupon is located at the top of glass airway I, and be conducive to cleaning glass airway I inwall, the liquid or clear water that contain sanitising agent are injected stopple coupon can be cleaned whole glass airway I inwall, and can not there is dead angle.Sampled by the ventilating pit angle that is provided with of adjustment sealing plug, need not extraction of closure plug, also close thief hole after convenient sampling, the flow velocity of gas sampled can also be controlled by the angle adjusting ventilating pit.
3, the sealing effectiveness of ground glass surface is better, convenient angle and the sampling repeatedly adjusting sealing plug.
A kind of bacteria culture bottle measuring gas production rate of the present utility model, because airway is glass material, thinner, free end is provided with wider scale, therefore the generation measuring a small amount of gas can be known, and there is wider scope, the upgrowth situation of bacterium and the nourishment of nutrient solution in culturing bottle can be known by the size of gas production rate; By sampling, composition and the content of gas in culturing bottle can also be detected, the upgrowth situation of bacterium in the nourishment of nutrient solution and culturing bottle can better be understood.The utility model can also control the temperature of nutrient solution, and the stirring being conducive to nutrient solution does not exist dead angle.The utility model structure is simple, good seal performance.The structure of airway, convenient cleaning airway inwall, and also there is a part of only replacing damage when damaging in any part, cost-saving.It is simple that this utility model belongs to a structure, the bacteria culture bottle of the convenient and practical gas production rate measured.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is a kind of structural representation measuring the bacteria culture bottle of gas production rate of the utility model.
Fig. 2 is a kind of structural representation measuring the glass airway I (6) of the bacteria culture bottle of gas production rate of the utility model.
Number in the figure: 1 is body, 2 is bottleneck, and 3 is bottle cap, 4 is interlayer, and 5 is small embossment, and 6 is glass airway I, 7 is stopple coupon, and 8 is sealing plug, and 9 is ventilating pit, 10 is sealing consent, and 11 is flexible pipe, and 12 is glass airway II, 13 is water inlet, and 14 is water outlet, and 15 is air guide pore, 16 is airway, and 17 is support.
Embodiment
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As shown in Figure 1-2, the bacteria culture bottle of gas production rate is measured for the present embodiment is a kind of, comprise body (1), bottleneck (2), bottle cap (3), airway (16), wherein, body (1) inwall is provided with vertical bar shaped small embossment (5), and small embossment (5) is arc with the contact surface of body (1) inwall; During liquid in stir culture bottle, small embossment (5) is conducive to stirring, and small embossment (5) is arc with the contact surface of culturing bottle inwall, and arc is conducive to the clean of culturing bottle, can not there is dead angle.Described body (1) is provided with interlayer (4), and interlayer (4) outer wall top is provided with water outlet (14), and interlayer (4) outer wall bottom is provided with water inlet (13); Sandwich structure can better keep the temperature of nutrient solution, the temperature of nutrient solution can also be controlled by the liquid adding certain temperature to water inlet (13), water inlet (13) under, water outlet (14) can better control upper the flow velocity adding liquid, makes the more uniform temperature of nutrient solution.Described bottleneck (2) is funnel-form, is conducive to lid bottle cap (3), also helps the sealing between bottle cap (3) and bottleneck (2).Described bottle cap (3) and bottleneck (2) are tightly connected, and bottle cap (3) is provided with air guide pore (15), is provided with airway (16) in air guide pore (15), described airway (16) comprises glass airway I (6), flexible pipe (11), glass airway II (12), be connected by flexible pipe (11) between glass airway I (6) with glass airway II (12), , described glass airway I (6) is inverted U structure, the free end of described glass airway I (6) stretches into body (1) inside through air guide pore (15), described glass airway I (6) is tightly connected with air guide pore (15), described glass airway II (12) is U-shaped structure, the free end of glass airway II (12) is provided with scale.Be connected by flexible pipe (11) between glass airway I (6) with glass airway II (12), facilitate the fixing of glass airway II (12), and glass airway II (12) fixing after, also can not the bottle cap (3) of inhibiting belt glass airway I (6) mobile, the scale be provided with by the free end of glass airway II (12) can read the size producing water displacement after gas, thus calculate the size of gas production rate, the upgrowth situation of bacterium and the nourishment of nutrient solution in culturing bottle can be known by the size of gas production rate; Airway (16) is divided into three parts, conveniently cleans airway (16) inwall, and a part of only replacing damage when damaging appears in any part, can be cost-saving.The present embodiment bottle cap (3) adopts polytetrafluoroethylmaterial material, is conducive to bottle cap with the sealing between bottleneck, also helps being tightly connected between air guide pore (15) and airway (16).The present embodiment bottle cap is polytetrafluoroethylmaterial material, is conducive to bottle cap and bottleneck, being tightly connected between air guide pore (15) and airway (16).
During use, glass airway I (6) is connected by flexible pipe (11) with glass airway II (12), the free end of glass airway I (6) is inserted in the air guide pore (15) of bottle cap (3), airway (16) is fixed, and injects appropriate amounts of sterilized water to the free end of glass airway II (12).Nutrient solution and bacterial classification are put into culturing bottle, build bottle cap (3) i.e. cultivable bacteria, the free end volume change of sterilized water at glass airway II can be read according to the vertical scale of glass airway II (12) free end, the gas production rate of culturing bacterium can be calculated by sterilized water at the volume volume change of glass airway II free end.If culturing bottle needs heating, the heat-conducting liquid of certain temperature can be flowed into from water inlet (13) and flow out heating culturing bottle from water outlet (14).
Further, the top of glass airway I (6) described in the present embodiment is provided with vertical stopple coupon (7), and described stopple coupon (7) middle part is provided with sealing consent (10); Sealing consent (10) is provided with supporting sealing plug (8), and sealing plug (8) middle part is provided with ventilating pit (9).The top that stopple coupon (7) is located at glass airway I (6) is conducive to cleaning glass airway I (6) inwall, the liquid or clear water that contain sanitising agent are injected thief hole (7) to clean whole glass airway I (6) inwall, and can not dead angle be there is.The angle adjusting ventilating pit (9) by adjustment sealing plug (8) samples, need not extraction of closure plug (8), can thief hole be closed after convenient sampling, the flow velocity of gas sampled can also be controlled by the angle of adjustment ventilating pit (9).
Further, sealing consent (10) described in the present embodiment with the contact surface of sealing plug (8) is ground glass surface, and the sealing effectiveness of ground glass surface is better, conveniently repeatedly adjusts the angle of sealing plug (8) and samples.Described glass airway II (12) is fixed by support (17).
Last it is noted that above embodiment is only in order to illustrate the technical solution of the utility model, be not intended to limit; Although be described in detail the utility model with reference to previous embodiment, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of each embodiment technical scheme of the utility model.

Claims (4)

1. can measure the bacteria culture bottle of gas production rate for one kind, comprise body (1), bottleneck (2), bottle cap (3), airway (16), it is characterized in that, described body (1) inwall is provided with vertical bar shaped small embossment (5), small embossment (5) is arc with the contact surface of body (1) inwall, described body (1) is provided with interlayer (4), described interlayer (4) outer wall top is provided with water outlet (14), and interlayer (4) outer wall bottom is provided with water inlet (13), described bottleneck (2) is funnel-form, described bottle cap (3) and bottleneck (2) are tightly connected, and bottle cap (3) is provided with air guide pore (15), is provided with airway (16) in air guide pore (15), described airway (16) comprises glass airway I (6), flexible pipe (11), glass airway II (12), be connected by flexible pipe (11) between glass airway I (6) with glass airway II (12), described glass airway I (6) is inverted U structure, the free end of described glass airway I (6) stretches into body (1) inside through air guide pore (15), described glass airway I (6) is tightly connected with air guide pore (15), described glass airway II (12) is U-shaped structure, the free end of glass airway II (12) is provided with vertical scale.
2. a kind of bacteria culture bottle measuring gas production rate according to claim 1, it is characterized in that, the top of described glass airway I (6) is provided with vertical stopple coupon (7), described stopple coupon (7) middle part is provided with sealing consent (10), sealing consent (10) is provided with supporting sealing plug (8), and sealing plug (8) middle part is provided with ventilating pit (9).
3. a kind of bacteria culture bottle measuring gas production rate according to claim 2, is characterized in that, described sealing consent (10) is ground glass surface with the contact surface of sealing plug (8).
4. a kind of bacteria culture bottle measuring gas production rate according to claim 1, is characterized in that, described glass airway II (12) is fixed by support (17).
CN201520136214.1U 2015-03-10 2015-03-10 A kind of bacteria culture bottle measuring gas production rate Expired - Fee Related CN204509338U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201520136214.1U CN204509338U (en) 2015-03-10 2015-03-10 A kind of bacteria culture bottle measuring gas production rate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201520136214.1U CN204509338U (en) 2015-03-10 2015-03-10 A kind of bacteria culture bottle measuring gas production rate

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CN204509338U true CN204509338U (en) 2015-07-29

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207354A (en) * 2018-11-19 2019-01-15 农业部规划设计研究院 A kind of microbial fermentation production potential test device
CN109370904A (en) * 2018-12-22 2019-02-22 精迅分子检测(深圳)有限公司 A kind of culture bottle with instruction device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207354A (en) * 2018-11-19 2019-01-15 农业部规划设计研究院 A kind of microbial fermentation production potential test device
CN109207354B (en) * 2018-11-19 2023-09-05 农业部规划设计研究院 Microorganism fermentation gas production potential testing device
CN109370904A (en) * 2018-12-22 2019-02-22 精迅分子检测(深圳)有限公司 A kind of culture bottle with instruction device

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150729

Termination date: 20170310

CF01 Termination of patent right due to non-payment of annual fee