CN204499258U - A kind of programmed cell freezing storing box of improvement - Google Patents
A kind of programmed cell freezing storing box of improvement Download PDFInfo
- Publication number
- CN204499258U CN204499258U CN201520047487.9U CN201520047487U CN204499258U CN 204499258 U CN204499258 U CN 204499258U CN 201520047487 U CN201520047487 U CN 201520047487U CN 204499258 U CN204499258 U CN 204499258U
- Authority
- CN
- China
- Prior art keywords
- frozen
- cryopreservation tube
- jack
- tube
- cryopreservation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Packages (AREA)
Abstract
The utility model is a kind of programmed cell freezing storing box of improvement, comprises container, frozen tube seat, frozen pipe support and lid; Frozen pipe support is provided with cryopreservation tube jack, pillar and hollow shadoof; Frozen tube seat there is circular groove face and from groove face to the annular outer wall of downward-extension, groove face is provided with cryopreservation tube corresponding aperture, pillar jack and shadoof, annular outer wall is provided with internal and external threads; Lid is provided with internal thread; Container is provided with external screw thread; Frozen tube seat is placed in above container, and frozen tube seat is connected by threaded engagement with container; Cryopreservation tube is placed on the top of frozen tube seat, and pillar inserts in pillar jack, and shadoof embeds in hollow shadoof, and cryopreservation tube jack is corresponding with cryopreservation tube corresponding aperture; Lid is placed in above frozen pipe support, and lid is connected by threaded engagement with frozen tube seat.The utility model can adapt to the batch access of cryopreservation tube; Meanwhile, can effectively prevent the volatilization of isopropyl alcohol and leak outside.
Description
Technical field
the utility model relates to biological consumptive material field, is specifically related to a kind of programmed cell freezing storing box of improvement.
Background technology
cell cryopreservation is an indispensable operating technology in cultured cell.The main object of freeze-stored cell is Cord blood seed cell, makes cell temporarily depart from growth conditions and be saved by its cell characteristics, uses after recovering at any time when needs; Main points of freeze-stored cell are that refrigerating process is wanted slowly.The method of tradition freeze-stored cell first in 4 DEG C of refrigerators, places 30--60 minute, then proceeds to-20 DEG C and place 30 minutes, then proceed to-80 DEG C of placements 16--18 hour (or spending the night), finally puts into the medium-term and long-term preservation of liquid nitrogen more as required.There is now programmed cell freezing storing box (under the effect of isopropyl alcohol, the speed cooling by 1 DEG C per minute can be reached, until less than-80 DEG C), the cell being added with cryopreserving liquid is placed in cell cryopreservation box can directly put into-80 DEG C spend the night after, just directly can put into the medium-term and long-term preservation of liquid nitrogen.Now cell cryopreservation box commercially assists by 100% isopropyl alcohol in container the object realizing slow cooling in cell freezing process, but isopropyl alcohol has toxicity, volatility, combustibility and easily absorb moisture, seal bad or improper use may cause and leak outside, therefore the sealing of isopropyl alcohol is the technical problem existed in existing program freezing storing box.Ordinary circumstance cryopreservation tube only has several milliliters, small volume, and in freezing storing box, several cryopreservation tubes of compact storage pick and place trouble sometimes simultaneously, therefore makes cryopreservation tube pick and place the convenient point being also existing program freezing storing box and needing to improve.Existing program freezing storing box mostly is 18 hole designs, the size in hole is identical, the frozen amount of each pipe is identical, freezing storing box can not meet meeting of cell sample different acquisition amount in experimentation, and the layout in hole is not suitable for fast, in large quantities cryopreservation tube is placed in-80 degree refrigerators or-196 degree liquid nitrogen are preserved.
Summary of the invention
The required technical problem solved of the utility model is providing the programmed cell freezing storing box that a kind of structure is simple, access convenience, good airproof performance, applicable quick, a large amount of frozen difference amount cell.The purpose of this utility model is achieved through the following technical solutions.
A programmed cell freezing storing box for improvement, comprises container, frozen tube seat, frozen pipe support and lid, and described frozen pipe support is provided with cryopreservation tube jack; Described frozen pipe support is provided with pillar and hollow shadoof; Described frozen pipe support inserts frozen tube seat and is connected with container upper end; Described frozen tube seat there is circular groove face and from groove face to the annular outer wall of downward-extension, described groove face is provided with cryopreservation tube corresponding aperture, pillar jack and shadoof, described annular outer wall is provided with internal and external threads; The outer wall inner edge of described lid is provided with internal thread; The outer wall outer rim of described container is provided with external screw thread; Described frozen tube seat is placed in above described container, and described frozen tube seat is connected by threaded engagement with described container; Described cryopreservation tube is placed on the top of described frozen tube seat, and described pillar inserts in described pillar jack, and described shadoof embeds in described hollow shadoof, and described cryopreservation tube jack is corresponding with described cryopreservation tube corresponding aperture; Described lid is placed in above described frozen pipe support, and described lid is connected by threaded engagement with described frozen tube seat.
Further, the varying in size of described cryopreservation tube jack.
Further, described cryopreservation tube jack is 24, and described hollow shadoof is arranged at the center of cryopreservation tube jack arrangement.
Further, cryopreservation tube jack is arranged in 5 row, and middle row arranges 4 cryopreservation tube jacks, each two the cryopreservation tube jacks in this journey left and right, and centre arranges described hollow shadoof; Other row are capable arrangement 5 cryopreservation tube jacks often.
Further, described pillar is 3.
Further, described pillar is 4.
The beneficial effects of the utility model are:
Compared with prior art, the cell program freezing storing box of a kind of improvement that the utility model provides, can disposable frozen more cryopreservation tube, and the batch using frozen pipe support can adapt to cryopreservation tube accesses repetitive operation when simplifying cell cryopreservation, reduces the time required for disposable frozen multiple cell cryopreservation tube; Meanwhile, in frozen tube seat outer wall, outside has screw thread, and Inner side screw thread can combine with container sealing, and outside screw can combine with lid sealing, can effectively prevent the volatilization of isopropyl alcohol and leak outside.
Below in conjunction with the drawings and the specific embodiments, the utility model is described in further detail.
Accompanying drawing explanation
Fig. 1 is the structural representation of the lid of the programmed cell freezing storing box of improvement of the present utility model.
Fig. 2 is the structural representation of the frozen tube seat of the programmed cell freezing storing box of improvement of the present utility model.
Fig. 3 is the structural representation of the frozen pipe support of the programmed cell freezing storing box of improvement of the present utility model
Fig. 4 is the structural representation of the container of the programmed cell freezing storing box of improvement of the present utility model.
Reference numeral:
1: lid, 11: lid internal thread; 2: frozen tube seat, 21: shadoof, 22: pillar jack, 23: cryopreservation tube corresponding aperture, 24: frozen tube seat external screw thread, 25: frozen tube seat internal thread; 3: frozen pipe support, 31: hollow shadoof, 32: pillar, 33: cryopreservation tube jack; 4: container, 41: container external screw thread.
Embodiment
The programmed cell freezing storing box of the improvement of the present embodiment, comprises lid 1, frozen tube seat 2, frozen pipe support 3, container 4.
The pipe support plane of described frozen pipe support 3 is for circle is (herein in order to join so circular with frozen tube seat, if 2 planes that the plane of frozen pipe support 3 is less than frozen tube seat select other shapes, also be fine as square), the pipe support in-plane of frozen pipe support 3 is provided with 24 cryopreservation tube jacks 33, cryopreservation tube can be inserted in cryopreservation tube jack 33, cryopreservation tube jack 33 is arranged in 5 row (as shown in Figure 3), become the arrangement of 5*5, the cryopreservation tube receptacle alignment that the cryopreservation tube jack of every a line is corresponding with other row, middle row arranges 4 cryopreservation tube jacks 33, each two the cryopreservation tube jacks 33 in this journey left and right, centre arranges described hollow shadoof 31, other row are capable arrangement 5 cryopreservation tube jacks 33 often.The pipe support horizontal edge of frozen pipe support 3 is evenly provided with 3 or 4 pillars 32.
Frozen tube seat 2 there is circular groove face and from groove face to the annular outer wall of downward-extension, groove face is provided with cryopreservation tube corresponding aperture 23, pillar jack 22 and shadoof 21, cryopreservation tube corresponding aperture 23 and cryopreservation tube jack 33 one_to_one corresponding on frozen pipe support 3, pillar jack 22 and pillar 32 one_to_one corresponding on frozen pipe support 3, shadoof 21 is corresponding with the hollow shadoof 31 on frozen pipe support 3.The inner side of frozen tube seat 2 annular outer wall is provided with frozen tube seat internal thread 25, and the outside of annular outer wall is provided with frozen tube seat external screw thread 24, corresponding with the screw thread on container 4 and lid 1 respectively.
The outer wall inner edge of lid 1 is provided with lid internal thread 11; The outer wall outer rim of container 4 is provided with container external screw thread 41.
Frozen tube seat 2 is placed in above container 4, and frozen tube seat internal thread 25 closely cooperates with container external screw thread 41, is tightly connected; Frozen pipe support 3 is placed in the top of frozen tube seat 2, and pillar 32 inserts in pillar jack 22.Shadoof 21 on frozen tube seat 2 embeds in the hollow shadoof 31 of frozen pipe support 3, and the cryopreservation tube jack 33 of frozen pipe support 3 is corresponding with the cryopreservation tube corresponding aperture 23 on frozen tube seat 2, and such cryopreservation tube is also inserted in the cryopreservation tube corresponding aperture 23 on frozen tube seat 2; Lid 1 is placed in above frozen pipe support 3, and lid internal thread 11 and the frozen tube seat external screw thread 24 of lid 1 outer wall inner edge are connected, and realize sealing.
On same frozen pipe support 24 cryopreservation tube jacks can size identical, also can vary in size.If not of uniform size, can be set to be applicable to respectively depositing 1.2ml, 1.5ml, 1.8ml, the different size cryopreservation tubes such as 2ml, are set to 5*5 arrangement mode in the present embodiment, middle row 4 cryopreservation tube jacks are 2mL cryopreservation tube jack, the first row and fifth line are cryopreservation tube jack is 1.2mL cryopreservation tube jack, and other two row are respectively 1.5mL and 1.8mL cryopreservation tube jack.
Claims (6)
1. the programmed cell freezing storing box improved, comprise container and lid, it is characterized in that: described freezing storing box also comprises frozen tube seat and frozen pipe support, described frozen pipe support is provided with cryopreservation tube jack, described frozen pipe support is provided with pillar and hollow shadoof, and described frozen pipe support inserts frozen tube seat and is connected with container upper end;
Described frozen tube seat there is circular groove face and from groove face to the annular outer wall of downward-extension, described groove face is provided with cryopreservation tube corresponding aperture, pillar jack and shadoof, described annular outer wall is provided with internal and external threads;
The outer wall inner edge of described lid is provided with internal thread;
The outer wall outer rim of described container is provided with external screw thread;
Described frozen tube seat is placed in above described container, and described frozen tube seat is connected by threaded engagement with described container; Described cryopreservation tube is placed on the top of described frozen tube seat, and described pillar inserts in described pillar jack, and described shadoof embeds in described hollow shadoof, and described cryopreservation tube jack is corresponding with described cryopreservation tube corresponding aperture; Described lid is placed in above described frozen pipe support, and described lid is connected by threaded engagement with described frozen tube seat.
2. programmed cell freezing storing box according to claim 1, is characterized in that, varying in size of described cryopreservation tube jack.
3. programmed cell freezing storing box according to claim 1, is characterized in that, described cryopreservation tube jack is 24, and described hollow shadoof is arranged at the center of cryopreservation tube jack arrangement.
4. programmed cell freezing storing box according to claim 3, is characterized in that, cryopreservation tube jack is arranged in 5 row, and middle row arranges 4 cryopreservation tube jacks, arranges described hollow shadoof in the middle of each two the cryopreservation tube jacks in this journey left and right; Other row are capable arrangement 5 cryopreservation tube jacks often.
5. programmed cell freezing storing box according to claim 1, is characterized in that, described pillar is 3.
6. programmed cell freezing storing box according to claim 1, is characterized in that, described pillar is 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520047487.9U CN204499258U (en) | 2015-01-23 | 2015-01-23 | A kind of programmed cell freezing storing box of improvement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520047487.9U CN204499258U (en) | 2015-01-23 | 2015-01-23 | A kind of programmed cell freezing storing box of improvement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204499258U true CN204499258U (en) | 2015-07-29 |
Family
ID=53697277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520047487.9U Expired - Fee Related CN204499258U (en) | 2015-01-23 | 2015-01-23 | A kind of programmed cell freezing storing box of improvement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204499258U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476955A (en) * | 2019-09-16 | 2019-11-22 | 上海理工大学 | A kind of programmed cell plant ice production apparatus |
-
2015
- 2015-01-23 CN CN201520047487.9U patent/CN204499258U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476955A (en) * | 2019-09-16 | 2019-11-22 | 上海理工大学 | A kind of programmed cell plant ice production apparatus |
| CN110476955B (en) * | 2019-09-16 | 2021-07-20 | 上海理工大学 | Cell procedure ice planting device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201752216U (en) | Program temperature reduction box | |
| CN106081363A (en) | A kind of liquid nitrogen zero is lost biological specimen low-temperature storing apparatus | |
| CN106442895A (en) | Testing device for testing low-temperature adsorption performance of gas | |
| CN204499258U (en) | A kind of programmed cell freezing storing box of improvement | |
| CN110583618B (en) | Efficient air-cooled gas-phase liquid nitrogen biological storage device and use method and application thereof | |
| CN103424319A (en) | Ultralow-temperature impact test device and test method thereof | |
| CN201322489Y (en) | Double-seal sperm cryopreservation tube | |
| CN204426477U (en) | A kind of Simple cell program freezing storing box | |
| CN209983409U (en) | Cytology is with portable cell cryopreserved pipe | |
| CN203040527U (en) | Cell freezing and storing box | |
| CN202538808U (en) | Sampling test tube device | |
| CN103979210B (en) | The long-term preservation method of a kind of plant sample and device | |
| CN204811679U (en) | Simple and easy multipurpose procedure cooling box | |
| CN208175928U (en) | A kind of cell save set | |
| CN205333348U (en) | Liquid is eaten quality control and is surveyed sample collection container | |
| CN203575473U (en) | Improved cell freezing box | |
| CN208211346U (en) | Gradient cooling box | |
| CN203745398U (en) | Rapid freeze thawing and single-edge freeze thawing all-in-one testing machine for concrete | |
| CN202590833U (en) | Test-tube rack used at low temperature | |
| CN203329745U (en) | Novel cryogenic device for protein purification | |
| CN207613034U (en) | A kind of adipose tissue cryopreservation tube | |
| CN204916647U (en) | Freezing storage device of partial shipment | |
| CN204907661U (en) | Gradient cooling box | |
| CN215775143U (en) | Cell preservation is with freezing pipe protection subassembly | |
| CN204157529U (en) | Segment type cryopreservation tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150729 Termination date: 20170123 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |