CN204369865U - A kind of kapillary gene-amplification system - Google Patents
A kind of kapillary gene-amplification system Download PDFInfo
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- CN204369865U CN204369865U CN201420809974.XU CN201420809974U CN204369865U CN 204369865 U CN204369865 U CN 204369865U CN 201420809974 U CN201420809974 U CN 201420809974U CN 204369865 U CN204369865 U CN 204369865U
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- kapillary
- temperature
- gene
- temperature cycling
- peristaltic pump
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A kind of kapillary gene-amplification system, it is characterized in that: comprise amplification mixing liquid bath, peristaltic pump, temperature cycling groove, kapillary, laser induced fluorescence detector, described amplification mixing liquid bath is connected with peristaltic pump entrance by pipeline, peristaltic pump outlet is connected with kapillary, described kapillary is arranged in temperature cycling groove ringwise, described temperature cycling groove comprises three thermostatic baths, and described kapillary is connected with laser induced fluorescence detector through after temperature cycling groove.The beneficial effects of the utility model are: provide a kind of kapillary gene-amplification system, structure is simple, easy to use, because kapillary passes in different sweat boxs, emulsion in kapillary is slow alternating temperature wherein, improve the activity of polysaccharase, and homogeneous temperature, operational efficiency is high, instrument volume is little, whole instrumentation cost is low, easy to maintenance, and a sense cycle only needs 30-40 minute.
Description
Technical field
The utility model relates to DNA cloning technical field, particularly relates to a kind of kapillary gene-amplification system.
Background technology
The technical equipment of gene amplification is made up of three parts: 1. template DNA required equipment, is mainly high speed micro whizzer or high speed freezing centrifuge; 2. PCR gene amplification instrument; 3. the interpretation of DNA cloning result and metering equipment, mainly contains Horizontal low pressure electrophoresis apparatus, PCR nucleic acid electrophoresis tank and ultraviolet transilluminator and DNA particle fluorescence meter etc.The technical scheme of gene-amplificative instrament can be summed up as two large classes: first kind scheme i.e. three container mechanical manipulator recycle schemes.The arrangement of such design consideration thermostatted can be divided into linear pattern and ring-like two kinds.The PCR instrument of program design arranges three accurate temperature controllings and the adjustable thermostatted of temperature, and maintains static, and makes sample disc under the mechanical manipulator effect of microcomputerized control, according to the soaking time needed for PCR reaction, stops and circulation between thermostatted.Equations of The Second Kind scheme and single vessel temp recycle scheme.Such scheme is in whole reaction process, and sample is invariant position in sample cell, and completes the conversion of temperature, maintenance and circulation by the lifting of Quality control groove temperature.
All things considered, the advantage of first kind scheme is: temperature transition is fast, and consuming time few, ramp rates can reach 10 DEG C/s, and thus temperature transition rate and DNA cloning efficiency are all higher than Equations of The Second Kind scheme.Temperature homogeneity more easily ensures, and very strong to the adaptive faculty of sample hose, can not only use centrifuge tube, thin-walled tube, and can do the capillary PCR of recent development and adopt the In situPCR of slide glass.Hardware aspect, because eliminating refrigerator, equipment is fairly simple, reduces cost and also easily controls and keep in repair, improve the reliability of instrument.Because the annealing temperature of PCR is usually all at 42 DEG C, thus the program does not affect effect and the quality of DNA cloning without the shortcoming of refrigerator.The weak point of the program is because gradient of temperature is too fast, may affect the activity of polysaccharase.In addition owing to saving refrigerator, make lowest temperature be subject to the restriction of envrionment temperature, the handiness of temperature program(me) is little, and instrument volume is larger.The advantage of Equations of The Second Kind scheme has been easy to alternating temperature owing to having introduced refrigerator, thus add the handiness of temperature inversion program, and instrument volume is also smaller.Main drawback is that temperature transition rate will be restricted owing to carrying out temperature cycle in a vessel, and temperature homogeneity and operational efficiency are all not as first scheme.At hardware aspect, if take compressor cooling will increase volume and cost; Adopt semiconductor cooler (Peltier element), then because thermal stresses acts on Peltier element face of weld, damage through repeatedly circulating causing face of weld to ftracture.The airbath of newly-developed, fluid cooling, kapillary fast PCR instrument, owing to adopting kapillary to make sample hose, utilize high velocity air to force cooling, in raising temperature transition rate and temperature homogeneity, propose new measure.
Utility model content
The utility model aims to provide a kind of kapillary gene-amplification system, solve the poor activity of the polysaccharase existed in current gene-amplificative instrament, temperature homogeneity and operational efficiency poor, instrument volume is large, arrange more complicated in instrument, and then cause whole instrumentation high in cost of production problem.
In order to solve the problem, the utility model provides a kind of kapillary gene-amplification system, it is characterized in that: comprise amplification mixing liquid bath, peristaltic pump, temperature cycling groove, kapillary, laser induced fluorescence detector, described amplification mixing liquid bath is connected with peristaltic pump entrance by pipeline, peristaltic pump outlet is connected with kapillary, described kapillary is arranged in temperature cycling groove ringwise, described temperature cycling groove comprises three thermostatic baths, and described kapillary is connected with laser induced fluorescence detector through after temperature cycling groove.
Preferably, described capillary diameter is 50-200 μm.
Preferably, the order that described kapillary passes three thermostatic baths in temperature cycling groove is successively temperature 96 DEG C of thermostatic baths, 60 DEG C of thermostatic baths, 72 DEG C of thermostatic baths, out from 72 DEG C of thermostatic baths finally.
The beneficial effects of the utility model are: provide a kind of kapillary gene-amplification system, structure is simple, easy to use, because kapillary passes in different sweat boxs, the emulsion in kapillary is slow alternating temperature wherein, improve the activity of polysaccharase, and homogeneous temperature, operational efficiency is high, and instrument volume is little, whole instrumentation cost is low, easy to maintenance.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Wherein: 1. amplification mixing liquid bath; 2. peristaltic pump; 3. temperature cycling groove; 4. kapillary; 5. laser induced fluorescence detector; 31.96 DEG C thermostatic bath; 32.60 DEG C thermostatic bath; 33.72 DEG C thermostatic bath.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described further.
As shown in Figure 1: the utility model provides a kind of kapillary gene-amplification system, it is characterized in that: comprise amplification mixing liquid bath 1, peristaltic pump 2, temperature cycling groove 3, kapillary 4, laser induced fluorescence detector 5, described amplification mixing liquid bath 1 is connected with peristaltic pump 2 entrance by pipeline, peristaltic pump 2 exports and is connected with kapillary 4, described kapillary 4 is arranged in temperature cycling groove 3 ringwise, described temperature cycling groove 3 comprises three thermostatic baths, i.e. 96 DEG C of thermostatic baths 31, 60 DEG C of thermostatic baths 32, 72 DEG C of thermostatic baths 33, described kapillary 4 is connected with laser induced fluorescence detector 5 through after temperature cycling groove 3.
Preferably, described kapillary 3 diameter is 50-200 μm.
Preferably, the order that described kapillary 3 passes three thermostatic baths in temperature cycling groove is successively temperature 96 DEG C of thermostatic baths, 31,60 DEG C of thermostatic baths 32,72 DEG C of thermostatic baths 33, out from 72 DEG C of thermostatic baths 33 finally.
Principle of work of the present utility model is: store the sample emulsion handled well in amplification mixing liquid bath 1, the sample emulsion pump that peristaltic pump 2 mixes handle well increasing in liquid bath 1 is in kapillary 4, emulsion is under the driving of peristaltic pump 2, enter into temperature cycling groove 3, DNA profiling depolymerization 96 DEG C time, 60 DEG C of primer annealing renaturation, 72 DEG C of primer extensions under archaeal dna polymerase effect, complete PRC amplification.Due to the restriction of the diameter of kapillary own, sample exists with the form of small droplets in kapillary 4, at most only a DNA chain is there is in each drop, ensure that the integrity of amplification, kapillary 4 to circulate 30-50 circulation at temperature cycling groove 3, completes the amplification of DNA chain, then through the detection of laser induced fluorescence detector 5, complete gene diagnosis, instruct doctor's medication, medical advice is provided.
The beneficial effects of the utility model are: provide a kind of kapillary gene-amplification system, structure is simple, easy to use, because kapillary passes in different sweat boxs, emulsion in kapillary is slow alternating temperature wherein, improve the activity of polysaccharase, and homogeneous temperature, operational efficiency is high, instrument volume is little, whole instrumentation cost is low, easy to maintenance, and a sense cycle only needs 30-40 minute.
Claims (3)
1. a kapillary gene-amplification system, it is characterized in that: comprise amplification mixing liquid bath, peristaltic pump, temperature cycling groove, kapillary, laser induced fluorescence detector, described amplification mixing liquid bath is connected with peristaltic pump entrance by pipeline, peristaltic pump outlet is connected with kapillary, described kapillary is arranged in temperature cycling groove ringwise, described temperature cycling groove comprises three thermostatic baths, and described kapillary is connected with laser induced fluorescence detector through after temperature cycling groove.
2. a kind of kapillary gene-amplification system according to claim 1, is characterized in that: described capillary diameter is 50-200 μm.
3. a kind of kapillary gene-amplification system according to claim 1 and 2, it is characterized in that: described kapillary passes the order of three thermostatic baths in temperature cycling groove successively for temperature 96 DEG C of thermostatic baths, 60 DEG C of thermostatic baths, 72 DEG C of thermostatic baths, out from 72 DEG C of thermostatic baths finally.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420809974.XU CN204369865U (en) | 2014-12-20 | 2014-12-20 | A kind of kapillary gene-amplification system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420809974.XU CN204369865U (en) | 2014-12-20 | 2014-12-20 | A kind of kapillary gene-amplification system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204369865U true CN204369865U (en) | 2015-06-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420809974.XU Expired - Fee Related CN204369865U (en) | 2014-12-20 | 2014-12-20 | A kind of kapillary gene-amplification system |
Country Status (1)
| Country | Link |
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| CN (1) | CN204369865U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674821A (en) * | 2017-11-16 | 2018-02-09 | 格致诊断公司 | Drop temperature cycles consersion unit |
-
2014
- 2014-12-20 CN CN201420809974.XU patent/CN204369865U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674821A (en) * | 2017-11-16 | 2018-02-09 | 格致诊断公司 | Drop temperature cycles consersion unit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150603 Termination date: 20161220 |