CN204332213U - A kind of chloroplast pigment experimental apparatus for filter paper chromatography - Google Patents
A kind of chloroplast pigment experimental apparatus for filter paper chromatography Download PDFInfo
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- CN204332213U CN204332213U CN201520005950.3U CN201520005950U CN204332213U CN 204332213 U CN204332213 U CN 204332213U CN 201520005950 U CN201520005950 U CN 201520005950U CN 204332213 U CN204332213 U CN 204332213U
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- chromatographic solution
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Abstract
A kind of chloroplast pigment experimental apparatus for filter paper chromatography, its structure comprises: base (3), paper chromatography cylinder (1), chromatographic solution conservation tank (2) and filter holder (10).Utilize the principle of linker to make chromatographic solution slowly flow to chromatography cylinder during experiment, chromatography phenomenon observed by the glass transparent by chromatography cylinder.This experimental provision is airtight, solves chromatographic solution volatilization and causes experimenter to suck the problem of chromatographic solution steam.Chromatographic solution is controlled slowly to flow to chromatography cylinder by valve (8), easily controls the degree of depth of chromatographic solution in chromatography cylinder, and filter paper immersion chromatographic solution can not be caused excessively deeply to cause the problem of pigmentolysis.The volume of chromatography cylinder is very little, well saves chromatographic solution, is flowed out, be beneficial to and recycle with the chromatographic solution crossed by sewer pipe, improves conventional efficient and success ratio simultaneously.
Description
Technical field
The utility model patent relates to a kind of biology experimental installation, specifically a kind of chloroplast pigment experimental apparatus for filter paper chromatography.
Background technology
In greenery, the Extraction and separation experiment of pigment is the required student experimenting of Senior Biology, and this experiment adopts silicon dioxide and calcium carbonate grinding greenery, adds absolute ethyl alcohol and extract pigment in greenery in process of lapping.On filter paper, draw a fine rule with extract, then filter paper is placed in chromatographic solution and carries out chromatography, according to chlorophyll a, chlorophyll b, xenthophylls and carotenoid different solubility in chromatographic solution, on filter paper, rate of propagation is different and separate.Chromatographic solution used is mixed by a certain percentage by sherwood oil, acetone and benzene, and these organic solvents are volatile, and has certain toxicity.Student gathers very near in an experiment due to the needs examined, often suck the organic solvent of these volatilizations, damage minor student is healthy.In order to avoid student sucks the chromatographic solution employing cotton balls of volatilization or the container of rubber stopper blocking dress chromatographic solution in prior art, there are following two shortcomings in this device: one, cotton balls or rubber stopper plug obtain not tight, prevent the poor effect of volatilizing, Sai get Tai is tight, chromatographic solution easily spills when extracting tampon or rubber stopper by student, causes more major injury.Two, the filter paper fine rule of chromatography is fixed on tampon or rubber stopper, is then suspended in chromatographic solution, and when filling in cotton balls or rubber stopper, the degree of depth of filter paper is not easy to control, if filter paper is put too deeply by pigmentolysis in chromatographic solution, can cause the failure of an experiment.
Summary of the invention
The utility model patent provides a kind of chloroplast pigment experimental apparatus for filter paper chromatography, and its structure comprises: base (3), paper chromatography cylinder (1), chromatographic solution conservation tank (2) and filter holder (10).Paper chromatography cylinder and chromatographic solution conservation tank are cylindrical, bottom is connected to form linker by a tubule, and tubule is installed chromatography hydraulic control valve door (8), each valve (6,7) installing an equilibrium air pressure above paper chromatography cylinder and chromatographic solution conservation tank, screw capping (4,5) is installed at paper chromatography cylinder and chromatographic solution conservation tank top, and waste liquid vent pipe and valve (9) are also installed by paper chromatography cylinder bottom portion.Filter holder adopts bolt (11) to be fixed on large intermediate plate by little intermediate plate (12), and filter holder is arranged on the inner side of screw capping (4), can take off with screw capping.Base adopts wood materials to make, and paper chromatography cylinder, chromatographic solution conservation tank and all valves adopt tempered glass to make, and top cover and filter holder adopt metal material to make.
Turn on chromatographic solution conservation tank screw capping during experiment, add 2/3 chromatographic solution, tighten screw capping.Turn on chromatography cylinder top cover, ready filter paper bar is fixed on filter holder, tightens screw capping.Open air pressure balance valve (6) and (7), slowly turn on chromatographic solution by-pass valve control (8) again, allow chromatographic solution slowly flow to chromatography cylinder, contact filter paper but valve-off (8), simultaneously closedown air pressure balance valve (6) and (7) when not touching chloroplast pigment fine rule until chromatographic solution.Chromatography phenomenon observed by the glass transparent by chromatography cylinder, until experiment terminates.After experiment terminates, air pressure balance valve (6) is opened, then open sewer pipe valve (9) Recycling of waste liquid.
The beneficial effects of the utility model are: this experimental provision is airtight, solve chromatographic solution volatilization and cause experimenter to suck the problem of chromatographic solution steam.Chromatographic solution slowly flows to chromatography cylinder by the principle of linker, easily controls the degree of depth of chromatographic solution in chromatography cylinder, and filter paper immersion chromatographic solution can not be caused excessively deeply to cause the problem of pigmentolysis.The volume of chromatography cylinder is very little, well saves chromatographic solution, is flowed out, be beneficial to and recycle with the chromatographic solution crossed by sewer pipe, improves conventional efficient and success ratio simultaneously.
Accompanying drawing explanation
Fig. 1 is a kind of chloroplast pigment experimental apparatus for filter paper chromatography longitudinal profile of the utility model structural map.
Fig. 2 is the schematic diagram of a kind of chloroplast pigment experimental apparatus for filter paper chromatography of the utility model in chromatography process.
Fig. 3 is a kind of chloroplast pigment experimental apparatus for filter paper chromatography of the utility model linker block diagram.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1-2, present embodiment chromatography cylinder (1) and chromatographic solution conservation tank (2) upper end uncovered, and the screw thread being provided with screw thread and screw capping (4) and (5) inner side matches.The internal diameter of chromatography cylinder is 30-80mm, and the internal diameter of chromatographic solution conservation tank is 100-400mm.Chromatography cylinder and chromatographic solution conservation tank adopt tempered glass to make, and are convenient to the lifting observing liquid level.
Embodiment two: composition graphs 3 illustrates present embodiment, an osculum is opened in present embodiment chromatography cylinder (1) and chromatographic solution conservation tank (2) bottom, is with the glass tube (15) of valve and rubber stopper (14) to be connected by one.Whole device is convenient to disassembly, cleaning.
Claims (5)
1. a chloroplast pigment experimental apparatus for filter paper chromatography, its structure comprises base (3), paper chromatography cylinder (1), chromatographic solution conservation tank (2) and filter holder (10), it is characterized in that: bottom paper chromatography cylinder and chromatographic solution conservation tank, be connected to form linker by a tubule, and tubule is installed chromatography hydraulic control valve door (8), each valve (6,7) installing an equilibrium air pressure above paper chromatography cylinder and chromatographic solution conservation tank, screw capping (4,5) is installed at paper chromatography cylinder and chromatographic solution conservation tank top, and waste liquid vent pipe and valve (9) are also installed by paper chromatography cylinder bottom portion.
2. chloroplast pigment experimental apparatus for filter paper chromatography according to claim 1, is characterized in that: filter holder adopts bolt (11) to be fixed on large intermediate plate by little intermediate plate (12), and filter holder is arranged on the inner side of top cover (4), can take off with screw capping.
3. chloroplast pigment experimental apparatus for filter paper chromatography according to claim 1, is characterized in that: base adopts wood materials to make, and paper chromatography cylinder, chromatographic solution conservation tank and all valves adopt tempered glass to make, and screw capping and filter holder adopt metal material to make.
4. chloroplast pigment experimental apparatus for filter paper chromatography according to claim 1, is characterized in that: paper chromatography cylinder, chromatographic solution conservation tank and the tubule both being connected are one-piece constructions.
5. chloroplast pigment experimental apparatus for filter paper chromatography according to claim 1, is characterized in that: the structure connecting paper chromatography cylinder and chromatographic solution conservation tank is dismountable structure, is made up of the glass tube of rubber stopper and band valve.
Priority Applications (1)
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CN201520005950.3U CN204332213U (en) | 2015-01-06 | 2015-01-06 | A kind of chloroplast pigment experimental apparatus for filter paper chromatography |
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CN201520005950.3U CN204332213U (en) | 2015-01-06 | 2015-01-06 | A kind of chloroplast pigment experimental apparatus for filter paper chromatography |
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CN201520005950.3U Expired - Fee Related CN204332213U (en) | 2015-01-06 | 2015-01-06 | A kind of chloroplast pigment experimental apparatus for filter paper chromatography |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106802335A (en) * | 2017-03-21 | 2017-06-06 | 天津科技大学 | A kind of automatic liquid control detachable flip type double flute chromatography cylinder |
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2015
- 2015-01-06 CN CN201520005950.3U patent/CN204332213U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106802335A (en) * | 2017-03-21 | 2017-06-06 | 天津科技大学 | A kind of automatic liquid control detachable flip type double flute chromatography cylinder |
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C14 | Grant of patent or utility model | ||
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150513 Termination date: 20170106 |
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CF01 | Termination of patent right due to non-payment of annual fee |