CN204298401U - A kind of steril cell returnable bottle - Google Patents
A kind of steril cell returnable bottle Download PDFInfo
- Publication number
- CN204298401U CN204298401U CN201420769689.XU CN201420769689U CN204298401U CN 204298401 U CN204298401 U CN 204298401U CN 201420769689 U CN201420769689 U CN 201420769689U CN 204298401 U CN204298401 U CN 204298401U
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- China
- Prior art keywords
- bottle
- cell
- bottle cap
- cone
- cap
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Abstract
The utility model relates to a kind of experiment using appts, is specifically related to a kind of steril cell returnable bottle used for placenta stem-cell lavation or other cells flushing separation.This steril cell returnable bottle, comprise at the bottom of bottle cap, bottle and bottle, described bottle is stamped bottle cap, bottle cap is provided with ventpipe and cell reclaims flexible rubber hose, ventilation pipe range 1cm, diameter 0.1mm, and cell reclaims flexible glue pipe range 5cm, diameter 0.5cm, described bottle is provided with scale marks, cell reclaims flexible rubber hose and is provided with pipe close, is cone at the bottom of described bottle, pointed end at the bottom of the bottle of cone.Its beneficial effect is: a kind of steril cell returnable bottle of the utility model, in the removal process of placenta stem-cell, can avoid microbiological contamination, leakage, the problems such as cell contamination effectively, experiment can be made convenient, fast, efficiently.
Description
Technical field
The utility model relates to a kind of experiment using appts, is specifically related to a kind of steril cell returnable bottle used for placenta stem-cell lavation or other cells flushing separation.
Background technology
New research proves containing abundant stem cell in placenta, especially the hemopoietic stem cell content several times of hemopoietic stem cell content in umbilical cord especially.At present, in the clinical application of hemopoietic stem cell, quantity is topmost restraining factors.As a kind of source of new hemopoietic stem cell, and abundant amount, the acquisition of convenience of drawing materials (placenta Chang Zuowei Biohazard Waste process) placental hematopoietic stem cell becomes more and more important.Verified in experiment, hemopoietic stem cell in placenta can lavation out, in Colony forming experiment, also demonstrating placental hematopoietic stem cell has into colony (CFU) ability, and the transplantation experiments in Immune deficient mice has proved the potentiality of placental hematopoietic stem cell in transplanting.Apply up-to-date method from placenta by hemopoietic stem cell lavation out, the recovery of cell needs a sterile environment, and the process reclaimed relates to the circulation etc. of air, so need an aseptic returnable bottle that can ventilate.
Current common way directly the recovery tube of lavation is positioned over the centrifugal bottle being exposed to air openings, like this, be easy in the process reclaimed, what have a bacterium etc. drops in centrifugal bottle, causes lavation to reclaim hemopoietic stem cell failure, makes a whole flow process failure in the end link; Also can exist and twist wrong time lid is screwed on, or twist and tight etc. do not cause leakage, microbiological contamination, reclaim cell failure.
Utility model content
The utility model, in order to make up the defect of prior art, provides and a kind ofly reclaims the steril cell returnable bottle used for placenta stem-cell lavation.
The utility model is achieved by the following technical solution:
A kind of steril cell returnable bottle, comprise at the bottom of bottle cap, bottle and bottle, described bottle is stamped bottle cap, bottle cap is provided with ventpipe and cell reclaims flexible rubber hose, ventilation pipe range 1cm, diameter 0.1mm, and cell reclaims flexible glue pipe range 5cm, diameter diameter 0.5cm, described bottle is provided with scale marks, cell reclaims flexible rubber hose and is provided with pipe close, is cone at the bottom of described bottle, pointed end at the bottom of the bottle of cone.
Further, be provided with two-layer air filter film in described ventpipe, air filter film lays respectively at ventpipe and bottle cap connectivity part and ventpipe end.
Further, described bottle cap is provided with the bottle cap screw thread of external spiral.
Further, on described bottle, bottleneck position is provided with the bottle screw thread of internal screw.
Further, described bottle cap and bottle are screwed up by the bottle cap screw thread of external spiral and the bottle thread helix of internal screw.
Further, the exterior contour of the body of described bottle is right cylinder, the exterior contour of bottleneck is right cylinder, the exterior contour of the bottle between bottleneck and body is Rotary-table, end face on the bottle that bottleneck is positioned at Rotary-table, cylindrical body is positioned at the Rotary-table bottom surface of bottle, is at the bottom of the bottle of cone bottom cylindrical body, pointed end at the bottom of the bottle of cone, the exterior contour that scale marks is positioned at bottle is Rotary-table end face and bottleneck junction.
The beneficial effects of the utility model are: a kind of steril cell returnable bottle of the utility model, in the removal process of placenta stem-cell, can avoid microbiological contamination, leakage, the problems such as cell contamination effectively, experiment can be made convenient, fast, efficiently.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is further described.
Accompanying drawing 1 is structural representation of the present utility model;
Accompanying drawing 2 is the structural representation of the utility model bottle cap;
Accompanying drawing 3 is the structural representation of the utility model bottle.
In figure, 1 bottle cap, 2 bottles, 3 cells reclaim flexible rubber hose, 4 pipe closes, 5 ventpipes, 6 scale markss, at the bottom of 7 bottles, 8 air filter films, 9 bottle cap screw threads, 10 bottle screw threads.
Embodiment
Accompanying drawing 1-3 is a kind of specific embodiment of the present utility model.A kind of steril cell returnable bottle of this utility model, to comprise at the bottom of bottle cap 1, bottle 2 and bottle 7, described bottle 2 is stamped bottle cap 1, bottle cap 1 is provided with ventpipe 5 and cell reclaims flexible rubber hose 3, the long 1cm of ventpipe 5, diameter 0.1mm, and cell reclaims the long 5cm of flexible rubber hose 3, diameter diameter 0.5cm, described bottle 2 is provided with scale marks 6, cell reclaims flexible rubber hose 3 and is provided with pipe close 4, and at the bottom of described bottle, 7 is cone, 7 pointed end at the bottom of the bottle of cone.
Further, be provided with two-layer air filter film 8 in described ventpipe 5, air filter film 8 lays respectively at ventpipe 5 and bottle cap 1 connectivity part and ventpipe 5 end.
Further, described bottle cap 1 is provided with the bottle cap screw thread 9 of external spiral.
Further, on described bottle 2, bottleneck position is provided with the bottle screw thread 10 of internal screw.
Further, described bottle cap 1 and bottle 2 are screwed up by the bottle cap screw thread 9 of external spiral and bottle screw thread 10 spiral of internal screw.
Further, the exterior contour of the body of described bottle 2 is right cylinder, the exterior contour of bottleneck is right cylinder, the exterior contour of the bottle 2 between bottleneck and body is Rotary-table, end face on the bottle 2 that bottleneck is positioned at Rotary-table, cylindrical body is positioned at the Rotary-table bottom surface of bottle 2, is at the bottom of the bottle of cone 7 bottom cylindrical body, 7 pointed end at the bottom of the bottle of cone, the exterior contour that scale marks 6 is positioned at bottle 2 is Rotary-table end face and bottleneck junction.
A kind of steril cell returnable bottle of this utility model, during use and placental hematopoietic stem cell lavage apparatus UNICOM, after sterilization, physiological saline circulates 10 minutes flushing pipes, then, after in access placenta to irrigation system, in aseptic operating platform, the cell opened on bottle cap 1 reclaims the pipe close 4 of flexible rubber hose 3, cell vein cells recovery tube be linked on bottle cap 1 reclaims on flexible rubber hose 3, fixing bottle 2, then constant current irrigation system is opened by cell harvesting in bottle 2, when the irrigating solution in bottle 2 reaches 90% time, close perfusion system, cell on bottle cap 1 is reclaimed flexible rubber hose 3 heat seal, disconnect with perfusion system again, irrigating solution in bottle is centrifugal, isolated cell, the mode that bottle cap 1 bottle 2 screws up for spiral, facilitate the use of aseptic returnable bottle, in order to the pollution of lavation process, increase ventpipe 5, in order to avoid polluting, after cell harvesting terminates, heat seal is returned cell and is reclaimed flexible rubber hose 3, reclaim for the ease of cell, the bottle end 7 is cone, in order to avoid reclaiming too much, bottle 2 there is scale marks 6, the equal medical plastic of whole parts of the present utility model makes, nontoxic aseptic single use.
The utility model is not limited to above-mentioned embodiment, anyone should learn make under enlightenment of the present utility model with the utility model, there is identical or close technical scheme, all fall within protection domain of the present utility model.
Technology, shape, structure part that the utility model is not described in detail are known technology.
Claims (6)
1. a steril cell returnable bottle, it is characterized in that, comprise at the bottom of bottle cap, bottle and bottle, described bottle is stamped bottle cap, bottle cap is provided with ventpipe and cell reclaims flexible rubber hose, ventilation pipe range 1cm, diameter 0.1mm, cell reclaims flexible glue pipe range 5cm, diameter diameter 0.5cm, and described bottle is provided with scale marks, and cell reclaims flexible rubber hose and is provided with pipe close, be cone at the bottom of described bottle, pointed end at the bottom of the bottle of cone.
2. bottle according to claim 1, is characterized in that: be provided with two-layer air filter film in described ventpipe, and air filter film lays respectively at ventpipe and bottle cap connectivity part and ventpipe end.
3. bottle according to claim 1, is characterized in that: described bottle cap is provided with the bottle cap screw thread of external spiral.
4. bottle according to claim 1 is characterized in that: on described bottle, bottleneck position is provided with the bottle screw thread of internal screw.
5. the bottle according to claim 3 or 4 is characterized in that: described bottle cap and bottle are screwed up by the bottle cap screw thread of external spiral and the bottle thread helix of internal screw.
6. bottle according to claim 1 is characterized in that: the exterior contour of the body of described bottle is right cylinder, the exterior contour of bottleneck is right cylinder, the exterior contour of the bottle between bottleneck and body is Rotary-table, end face on the bottle that bottleneck is positioned at Rotary-table, cylindrical body is positioned at the Rotary-table bottom surface of bottle, be at the bottom of the bottle of cone bottom cylindrical body, pointed end at the bottom of the bottle of cone, the exterior contour that scale marks is positioned at bottle is Rotary-table end face and bottleneck junction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420769689.XU CN204298401U (en) | 2014-12-10 | 2014-12-10 | A kind of steril cell returnable bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420769689.XU CN204298401U (en) | 2014-12-10 | 2014-12-10 | A kind of steril cell returnable bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204298401U true CN204298401U (en) | 2015-04-29 |
Family
ID=53103958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420769689.XU Expired - Fee Related CN204298401U (en) | 2014-12-10 | 2014-12-10 | A kind of steril cell returnable bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204298401U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625908A (en) * | 2021-01-11 | 2021-04-09 | 长春生物制品研究所有限责任公司 | Aseptic combination formula silica gel cell factory bolt |
-
2014
- 2014-12-10 CN CN201420769689.XU patent/CN204298401U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625908A (en) * | 2021-01-11 | 2021-04-09 | 长春生物制品研究所有限责任公司 | Aseptic combination formula silica gel cell factory bolt |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150429 Termination date: 20151210 |
|
| EXPY | Termination of patent right or utility model |