CN204298379U - The portable extraction element of rapid extraction nucleic acid - Google Patents
The portable extraction element of rapid extraction nucleic acid Download PDFInfo
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- CN204298379U CN204298379U CN201420507166.8U CN201420507166U CN204298379U CN 204298379 U CN204298379 U CN 204298379U CN 201420507166 U CN201420507166 U CN 201420507166U CN 204298379 U CN204298379 U CN 204298379U
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Abstract
The utility model provides the apparatus and method of preparation and extraction nucleic acid fast from biomaterial.In one embodiment, extraction element of the present utility model comprises extraction pylon, a collection group and a heating group.In another embodiment, extraction element of the present utility model comprises extraction pylon, a collection group, a heating group and a vacuum group.In one embodiment, extraction element of the present utility model and other device or instrument conbined usage, such as flow hybridization device, suction generating device or automatic gear.
Description
Technical field
The present invention relates to the method and apparatus of a kind of quick preparation and extraction nucleic acid.
Background technology
In clinical trial, from biological specimen, extract nucleic acid be a routine but very important step.Quality and the efficiency of extraction are very relevant to clinical trial.There are many method and apparatus now for extracting the nucleic acid in biomaterial.One of technology wherein the most often used is the extraction system based on silica and extraction column.In the art, pellosil is embedded in cylinder or extraction column.With the use of suitable damping fluid, we can from biomaterial by nucleic acid extraction out, make nucleic acid absorption on film subsequently.Conbined usage glued membrane and post can make sample and damping fluid pass film fast and fully under the effect of certain motivating force (as centrifugal force), thus accelerate extraction rate and improve the purity of nucleic acid.But carrying out extraction step still needs to spend consumption many time and effort.Such as, in the process extracted, add sample and damping fluid in extraction column, after often taking turns washing and centrifugal step, discard the water conservancy diversion thing supervisor through film, be all the committed step needing manually to carry out.Time and the program of simplifying the operation can improve leaching process, the efficiency of even clinical trial widely.In addition, shortening whole leaching process can decreasing pollution nucleic acid widely.Therefore, need a set of more high-level efficiency and automation equipment and method to extract nucleic acid.
At present in order to diagnose the illness or analyzing gene (such as single nucleotide polymorphism analysis Single Nucleotide Polymorphism Profiling and genotype identification), clinical and demand that is genetic test constantly increases, Gao Xiaoti get ﹑ expand increase ﹑ detect and/or analysis of nucleic acids extremely important to dealing with regarding demand.Therefore, need to research and develop instrument and system to simplify the program extracting nucleic acid from different biological sample, and be directly transported to next treatment step and such as expand extracting the nucleic acid obtained and increase ﹑ restriction enzyme and cut or mark the efficiency helping and improve test.
Have now much semi-automatic and with vacuum controlled instrument and device for extracting DNA (DNA) and/or the ribonucleic (RNA) of biological sample.
United States Patent (USP) 8,658, No. 105 (Taigen Biological Science Co., Ltd, Taigen Bioscience Corporation) describe the instrument of an energy safe handling biological sample, this instrument can be used for Qing Xi ﹑ isolated or purified biomolecules, such as DNA, RNA and protein.This instrument comprises the post ﹑ vacuum manifold and at least one non-essential adapter composition that assemble semipolar linkage.When vacuum-treat vacuum manifold, the liquid in semi-permeable membranes post is pumped to outside post by film.VacEZor (Taigen Biological Science Co., Ltd) can extract DNA and RNA (at every turn can extract at most 36 samples) when semi-automatic and vacuum.VacEZor allows that Biomolecular adsorption and washing step carry out on vacuum manifold, and does not need to assemble adsorption column centrifugal with repeating when discarding waste liquid.Each sample is independent processing, thus avoids crossed contamination.
QIAvac 24Plus (Qiagen) is a vacuum motor that simultaneously can process 24 adsorption columns.Similar to VacEZor, this device by being omitted in loading and unloading adsorption column on whizzer, and discards the step of water conservancy diversion thing in absorption and washing procedure, shortens the time of extracting nucleic acid.QIAvac 96 (Qiagen) is another vacuum motor, can process 96 samples simultaneously.This device in operation, can add extra Zu Jian ﹑ waste liquid dish or collection container (as collected microtubule or 96 orifice plates), convenient sample From assembly is transported to another assembly, thus mark during Bi Mian Charges and transport step.
Although the above description of device can reduce the time of the adsorption column and waste liquid treatment, the use of these devices is still very limited, because they cannot be applied to biological samples in advance handler, which reduces the efficiency of extraction.Anticipate in program described, cell in biological sample is cleaved, and in other tubule individual or instrument, using specific reagent to remove genosome DNA ﹑ core acid enzyme other enzymes of ﹑ or the protein of large fragment with group point ﹑ in release cells, these materials all may have influence on nucleic acid purification process subsequently.The non-purified extract obtained from the program of anticipating is transferred to the above-mentioned device mentioned subsequently further to purify.
Sometimes need to utilize heating power to accelerate the cracking process of cell, particularly process the time marquis of viscous samples (as phlegm).Therefore, be equipped with calorifier, and the extraction element of bind nucleic acid extraction and nucleic acid treatment step subsequently can help to reach to more high efficiency experiment and analysis.The invention provides a portable nucleic acid-extracting apparatus, this device gets Zhuan Zhi ﹑ guiding device and Heating element in conjunction with Ti, has exempted from but the program of anticipating biological sample, and can combine with nucleic acid handling procedure subsequently.By accelerating nucleic acid extraction and process subsequently and analysis, the present invention can improve clinical and efficiency that is genetic test.
Utility model content
The invention provides simple and quick for extracting the method and apparatus of biological sample nucleic acid.
In one embodiment, extraction element of the present invention comprises extraction pylon, a collection group and a heating group.
In one embodiment, extraction element of the present invention comprises extraction pylon, a collection group, a heating group and a vacuum group.
In one embodiment, extraction element of the present invention can combine with other devices or instrument and use, and such as water conservancy diversion hybridization Zhuan Zhi ﹑ produces suction device or electro-heat equipment.
In one embodiment, the invention provides use extraction element of the present invention to extract the method for nucleic acid.
The invention provides the method and apparatus simply and being fast same as and extracting biological sample nucleic acid.
In one embodiment, extraction element of the present invention can be combined to extract nucleic acid with any suitable extraction Zhu ﹑ Zhu ﹑ Ping ﹑ reagent such as Xi cellular lysate Ye ﹑ Xi Di Ye ﹑ elutriant or other similar materials.In another embodiment, extraction element of the present invention can be combined with other devices or system, to process or to analyze the nucleic acid extracting and obtain further.
In one embodiment, extraction element of the present invention may be used for being combined with other devices or instrument such as flow hybridization device and reverse dot blot hybridization (ASO-RDB) flow hybridization device.In one embodiment, described device can with United States Patent (USP) 5,741, No. 647, United States Patent (USP) 6,020, No. 187 and method described by PCT application publication No. WO/2011/139750 or device, or any can carry out flow hybridization novel method or device be combined.In one embodiment, flow hybridization device is FT-PRO flow hybridization system (Diagcor Bioscience Inc. Ltd., DiagCor Bioscience Incorporation Limited).
In one embodiment, the device that device of the present invention can generate heat or produce suction with other is combined.
In one embodiment, extraction element of the present invention can be combined with other automatizations or mechanised installation or system, extracts the program of nucleic acid and process nucleic acid with automatization.
The invention provides one for extracting the nucleic acid-extracting apparatus of nucleic acid, forming by with lower part:
I) extract pylon, comprise one or more perforate mouth, for supporting one or more extraction column, described extraction column contains sample, reagent or its combination;
Ii) collection group, comprises one or more grooves at an upper portion thereof, is used for supporting one or more collection tube, and described collection tube receives liquid from extraction column or the sample that contains containing nucleic acid;
Iii) heating group, comprises one or more heating chamber, for controlling the temperature of collection tube; The bottom of described extraction pylon is placed on the groove of described collection group, and the bottom of described collection group comprises one or more coupling device, is used for being connected with the heating chamber of described heating group.
In one embodiment, device of the present invention also comprises the lid that covers extraction pylon top.
In one embodiment, device of the present invention also has a waste liquid tank between extraction pylon and collection group, and for collecting from extraction column waste liquid out, described waste liquid tank comprises one or more waste liquid barrel, the groove in the collection group described in being used for connecting.
In one embodiment, device of the present invention also comprises one or more O type ring in following one or more position:
I) extract between pylon and collection group; And
Ii) between the coupling device of collection group and heating group.
In one embodiment, the extraction pylon of apparatus of the present invention is on perforate mouth side or contain one or more slot between described perforate mouth, and each described slot embeds extraction column Heating element.
In one embodiment, the extraction column Heating element of apparatus of the present invention is a kind of resistance heating elements, and described resistance heating element is selected from metal Fa Re Yuan Jian ﹑ ceramic heating element or synthesis Heating element.
In one embodiment, the extraction column of apparatus of the present invention is equipped with an adapter, and for containing sample or the reagent of more volume, described adapter connects with the opening of described extraction column.
In one embodiment, the collection group of apparatus of the present invention is a waste collection Zu ﹑ sample collection Zu ﹑ wash-out group or sample recovery group.
In one embodiment, the heating group of device of the present invention comprises one or more Heating element, and described Heating element is arranged on the below of the heating chamber in heating group.
In one embodiment, the Heating element of apparatus of the present invention is selected from metal Fa Re Yuan Jian ﹑ ceramic heating element and synthesis Heating element.
In one embodiment, device of the present invention comprises a vacuum group combined with suction generating device further, described vacuum group comprises the opening that has multiple holes head, and this some holes head allows air to pass through wherein, and the bottom of wherein heating group is embedded in the opening of described vacuum group.
In one embodiment, device of the present invention also comprises one or more O type ring, is placed on in following one or more position:
I) extract between pylon and collection group;
Ii) between the coupling device of collection group and heating group; And
Iii) between heating group and vacuum group.
In one embodiment, the extraction column used together with device of the present invention is embedded in the filter membrane or film that are applicable to extract nucleic acid.In another embodiment, sample or reagent, or it is combined through extraction column arrival collection tube or waste liquid tank.
In one embodiment, device of the present invention is used for extracting nucleic acid from biological sample.In another embodiment, described biological sample is Xue Ye ﹑ Xue Qing ﹑ Xue Jiang ﹑ Tuo Ye ﹑ Tan ﹑ Jing Ye ﹑ lymph liquid, or other body fluid.
In one embodiment, device of the present invention can hybridize the reverse spot flow hybridization device of Zhuan Zhi ﹑ with water conservancy diversion, or any can carry out flow hybridization device or instrument be combined.
In one embodiment, device of the present invention can be combined with electro-heat equipment or hot-plate.In another embodiment, device of the present invention can be combined with the system of an automatization or mechanize.
In one embodiment, extraction element as shown in Figure 1 includes at least three functional group:
1. extract pylon (10), for supporting multiple extraction column (11), wherein sample or reagent join in extraction column;
2. collection group (20), to support multiple collection tube (21), collection tube (21) is for collecting the material of discharging from the extraction column extracted pylon; And
3. heating group (30), for providing the temperature of heating power or a certain scope of holdout device for device.
In one embodiment, extract pylon (10) to combine with the top of collection group (20), wherein said collection group (20) and then combined by the top of coupling device (22) with heating group (30).Multiple groove (23) is contained in the upper end of collection group (20), for supporting collection tube (21).The lower end of extracting pylon (10) is placed on the upper end of collection group (20), thus makes the collection tube (21) be placed in the groove (23) of collection group (20) combine with extraction column (11).In one embodiment, the lower end of collection group (20) comprises multiple coupling device (22), can insert in the heating chamber (31) of the upper end being in heating group (30).
In one embodiment, extraction pylon (10) of the present invention comprises multiple hollow tube chamber (12), according to the needs of test, can hold extraction post ﹑ post or the bottle of all size.Described extraction Zhu ﹑ post or bottle can by the types of its material to be processed or nucleic acid, are packed with different types of adsorbent and permeable mass mutually as silica.Extract pylon (10) and any suitable form can be designed to, as the form of " nxm " or the form of linear array.In one embodiment, extract the form (Fig. 2) that pylon is " 4x4 ", therefore can extract the nucleic acid of 16 samples simultaneously.In another embodiment, extraction pylon (10) top of extraction element also comprises a lid or cover.Extraction pylon (10) shown in Fig. 2 is combined by upper assembly (13) and lower assembly (14), wherein goes up assembly (13) and lower assembly (14) is complete complementary.
Extract pylon (10) to be made up of both or the other materials moulding material ﹑ gold genus ﹑ plastics and metal.In one embodiment, extracting pylon (10) is one piece design (Fig. 3 A), by metal as aluminium ﹑ stainless steel or alloy are made.In one embodiment, extract pylon (10) to design (Fig. 3 B) for two pieces type, be made up of upper assembly (13) and lower assembly (14), upper assembly by thermoplastic plastic such as polypropylene (PP) ﹑ polycarbonate (PC) or acrylonitrile butadiene styrene (ABS) are made, and lower assembly You Lv ﹑ stainless steel or alloy are made.
In order to accelerate lysis or maintain sample in certain temperature, extraction pylon (10) of the present invention also can comprise one or more extraction column Heating element (15), for the sample in extraction column (11) provides or produces heat.In one embodiment, extract pylon (10) and comprise one or more slot (16) be placed between hollow tube chamber (12) side or tube chamber (12) ranks, slot (16) is for receiving extraction column Heating element (15).In extraction pylon (10) shown in Fig. 4, the hollow tube chamber (12) often arranged is sandwiched between two slots (16).In one embodiment, extraction column Heating element (15) is embedded in the one or more slots (16) extracted in pylon (10).In one embodiment, temperature range is 25 DEG C-95 DEG C.
Extraction column Heating element (15) can be any Heating element, such as resistance heating element, includes but not limited to metal Fa Re Yuan Jian ﹑ ceramic heating element and synthesis Heating element.In one embodiment, extraction column Heating element (15) is printed circuit board (PCB), soft PCB or ceramic heating.
In one embodiment, as shown in Figure 5, the adapter (17) of suitable size can put into the opening of extraction column (11), to hold the liquid of more volume and to make it be able to through extraction Zhu ﹑ post or bottle.In one embodiment, adapter (17) is 100 μ L ﹑ 200 μ L ﹑ 500 μ L ﹑ 1mL ﹑ 1.5mL or 2mL.Adapter (17) can be made up of different plastics, such as polypropylene (PP) and polycarbonate (PC) etc.
In whole leaching process, dissimilar collection group (20) and collection tube (21) can be used in response to indivedual extraction step.The groove (23) of collection group (20) can have multiple different diameter and curvature, to support the collection tube (21) of suitable type.In one embodiment, collection group (20) be one for collecting waste liquid or other throw aside the waste collection group of material.In another embodiment, collection group (20) be one for collecting the sample collection group of eluate or other useful matteies.The collection group with other designs also together can use to coordinate different experiment purposes from the present invention.Such as introduce sample to extraction column time, can use sample recovery group with collect first wash-out afterwards from the diversion fluid of extraction column.The diversion fluid collected again can be introduced in same extraction column and take turns combination to carry out second, to increase the extracted amount of nucleic acid as far as possible.In another embodiment, collection group (20) is a wash-out group, for collecting from the eluate in extraction column.Described wash-out group can support tubule or other containers, and these tubules or container are equipped with suitable material to process the nucleic acid of extraction further.Such as, the 0.5mL tubule containing suitable agent can be placed in wash-out group, cut to carry out enzyme to nucleic acid.
Collection group (20) can be made up of any material, the combination of such as Su Liao ﹑ Jin Shu ﹑ plastics and metal or other materials.In one embodiment, collection group (20) by plastics such as polypropylene, ((make PP) ﹑ polycarbonate by PC) ﹑ acrylonitrile butadiene styrene (ABS) or Teflon (Teflon).In another embodiment, collection group (20) is made up as Lv ﹑ stainless steel or alloy of metal.
According to the design of collection group (20), the collection tube (21) of different size or capacity can be used.In one embodiment, the capacity of the collection tube (21) used together with the present invention is 100 μ L, 200 μ L, 500 μ L, 1.5mL or 5mL.As shown in fig. 1, the collection tube (21) of 1.5mL is placed in the groove (23) of this device.As shown in Figure 6, the collection tube (21) of 5mL is placed in the groove (23) of this device.
In one embodiment, extraction element also comprises a waste liquid tank (40), waste liquid tank (40) is placed on and extracts between pylon (10) and collection group (20), for collecting from extraction column (11) waste liquid out or the material (Fig. 7) thrown aside.In one embodiment, waste liquid tank (40) is a removal waste fluid bucket (41).Waste liquid barrel (41) can be placed in the groove (23) of collection group (20) or be placed in the collection tube (21) in described groove (23).Difference can be coordinated to need the waste liquid barrel (41) of design and use different diameter and size.
Heating group (30) of the present invention is a heat-conducting medium that can generate heat or keep certain temperature, to promote that nucleic acid extraction and the program of carrying out subsequently are as nucleic acid isothermal amplification.Heating group (30) can be made up of any metallic substance, such as Lv ﹑ stainless steel or alloy.In one embodiment, heating group (30) is made by corrosion resistant metal or through the metal of corrosion-resistance treatment.In one embodiment, the top of heating group (30) comprises multiple heating room (31), and the groove (23) of this heating room and collection group (20) matches.
In one embodiment, heating group (30) can with one allot outward thermal with the use of, give sample (Fig. 8 A) to provide heating power.In one embodiment, outer allocation hot charging is set to guiding device or heating board.The flat bottom of heating group (30) is directly placed on the top of described electro-heat equipment.Heating power transfers to the bottom of heating group by described electro-heat equipment, finally arrives collection tube (21), and wherein collection tube (21) is inserted in heating room (31) by coupling device (22).
In another embodiment, heating group (30) also comprises one or more Heating element (32) being arranged on heating group (30) bottom, can provide heating power when not connecting outer allocation thermal.As shown in Figure 8 B, individual other Heating element (32) is arranged on the below of the heating room (31) in heating group (30), to provide heating power directly to sample.Heating element (32) also comprises the secondary Heating element be placed near collection tube.Heating element (32) can be any Heating element, such as resistance heating element, includes but not limited to metal Fa Re Yuan Jian ﹑ ceramic heating element and synthesis Heating element.In one embodiment, Heating element (32) is thermoelectric cooling module (Thermoelectric Cooling, TEC), printed circuit board (PCB), flexible circuit board or ceramic heating.
According to the needs of leaching process, outside no matter whether using, allot thermal, can arrange by heating group (30) and maintain different temperature, thus improve the speed extracted or process nucleic acid.In one embodiment, the scope of temperature is 25 DEG C-95 DEG C.
By maintaining a heating environment, device of the present invention can promote the cracking of cell, particularly from the cell of the phlegm compared with thickness.Heating environment can also accelerate the volatilization of ethanol in sample, and this step is extremely important, because ethanol often can reduce the quality of extracted nucleic acid, and affects the effect of nucleic acid treatment step subsequently.In addition, increase ﹑ enzyme with post-processing step as expanded and cut and mark, usually need the temperature that specific or raise.These later step are allocated suitable temperature by heating group (30) and are directly carried out after completing extraction.This can shorten the time of process nucleic acid and to lower between sample crossed contamination or fall random possibility, therefore makes clinical or genetic test more accurately and efficient.
In one embodiment, device of the present invention can be driven by a suction generating device, such as peristaltic pump (peristaltic pump) or surge pump (diaphragm pump), by liquid picking device and withdrawing device to accelerate leaching process.In one embodiment, device of the present invention also comprises a vacuum group (50), and this vacuum group (50) is for connecting heating group (30) and suction generating device.As shown in Figure 9, device of the present invention comprises extraction pylon (10), a collection group (20), a heating group (30) and a vacuum group (50).Extraction column (11) and the collection tube (21) of dissimilar or size can be used according to the particular requirement of leaching process.
As shown in Figure 9, device of the present invention is by connecting extraction pylon (10) of the present invention, collection group (20) and heating group (30) and make, wherein vacuum group (50) is directly placed on the bottom of heating group (30).In one embodiment, heating group (30) is embedded in the opening (51) of vacuum group (50), described vacuum group opening (51) is defined by the upper end of vacuum group (50) outer wall, and with the shape complementarity of heating group.
In one embodiment, the plane of vacuum group opening (51) comprises multiple holes head, and air can passing hole head thus through vacuum group (50), aspirate with the carrying out in device.
In one embodiment, connect suction generating device to aspirate, thus make liquid stream through device of the present invention.In another embodiment, between heating group (30) and vacuum group (50), also placed a valve.When suction unit operates, in device, one negative pressure can be formed.Once valve open, just can aspirate, thus make liquid stream through device.
Vacuum group (50) can be made up of any material, the combination of such as Su Liao ﹑ Jin Shu ﹑ plastics and metal or other materials.In one embodiment, vacuum group (50) by plastics such as polypropylene, (PP) ﹑ polycarbonate (PC), make by acrylonitrile butadiene styrene (ABS) or Teflon (Teflon).In another embodiment, vacuum group (50) is made up of metal such as Lv ﹑ stainless steel or alloy.
In one embodiment, as shown in Figure 10, the inventive system comprises with lower component:
1. extract pylon (10);
2. extract pylon O type ring (18);
3. collection group (20);
4. heating group O type ring (33);
5. heating group (30);
6. vacuum group O type ring (52); And
7. vacuum group (50).
As shown in Figure 10, device of the present invention is first by extracting pylon O type ring and will extract pylon (10) and collection group (20) combining.Extract pylon O type ring (18) and there is retractility and elasticity, can be centered around on the outward flange of extraction pylon (10) and collection group (20).The edge at the edge and collection group (20) top that extract pylon (10) bottom can comprise groove further to add stressed joint extraction column frame O type ring (18).Then, the coupling device (22) of collection group (20) is directly connected with the heating room (31) of heating group (30), and the edge at heating room (31) top contains a groove with compact siro spinning technology heating group O type ring (33).The planar base of heating group (30) is connected with the vacuum openings in vacuum group (50) by vacuum O type ring (52) subsequently.Vacuum group O type ring (52) is centered around on the outward flange of heating group (30) and vacuum group (50).
In one embodiment, extract pylon O type ring (18) ﹑ heating group O type ring (33) and vacuum O type ring (52) is made up of silicon rubber glue ﹑ rubber or other plastics, for sealing space between each merit energy Group in case leak-stopping gas.
According to the chi inch of Gong Neng Group, the O type ring of different diameter and girth can be used.In one embodiment, the diameter extracting pylon O type ring (18) is 3mm, and girth is 438.13mm.In one embodiment, the diameter of heating group O type ring (33) is 1mm, and girth is 182.08mm, can use one or more heating group O type ring (33).In one example, the diameter of vacuum group O type ring is 3mm, and girth is 414.13mm.
Different Gong Neng Zu ﹑ pipe/bottles and reagent is closed, the multiple steps during device of the present invention can extract by giving.In one embodiment, device of the present invention at least comprises one and extracts pylon (10), and (waste liquid) collection group (20), a heating group (30), for anticipating sample and lysing cell.In another embodiment, device of the present invention at least comprises one and extracts pylon (10), (sample) collection group (20) and a heating group (30), be adsorbed on the nucleic acid in extraction column for separating of He Suan ﹑ washing and wash-out.Vacuum group (50) and suction generating device can according to the actual step choice for uses extracted.
The present invention can extract nucleic acid from multiple different material, includes but not limited to DNA ﹑ RNA or other nucleic acid.In one embodiment, described material is a biological sample, includes but not limited to Xue Ye ﹑ Xue Qing ﹑ Xue Jiang ﹑ Tuo Ye ﹑ Tan ﹑ Jing Ye ﹑ lymph liquid or other body fluid.In one embodiment, described material obtains Cong Ren Ti ﹑ non-human animal or non-animal.In the present invention, dissimilar extraction pylon (10) and collection group (20) can be designed the material to coordinate particular type.
In one embodiment, the invention provides the method for extracting nucleic acid using apparatus of the present invention.
Experimental detail following by reference, the present invention may be better understood.But, it will be understood by those skilled in the art that provided embodiment is only as illustration, but not limit the scope of the invention.Scope of the present invention defined by claim subsequently.
In this application, refer to different reference or publication.The full text of these references or publication is all attached in the application with open, thus more fully describes situation of the present invention.It should be pointed out that Transitional Language " comprise " with ' comprise ’ ﹑ ' containing ' or ' with ... for feature ' be synonym, comprising property or open, does not centrally get rid of the element or method steps that have and do not enumerate in addition.
Accompanying drawing explanation
Fig. 1 is one of them extraction element of the present invention.
Fig. 2 shows the extraction pylon that has 4X4 form.
Fig. 3 A shows the extraction pylon of a single-piece.Fig. 3 B shows the extraction pylon of a two pieces type.
Fig. 4 shows the extraction pylon that is embedded with extraction column Heating element and slot.
Fig. 5 shows an adapter and extraction column, and the top of this extraction column placed the adapter for containing sample or reagent.
Fig. 6 is an extraction element being furnished with collection tube, and this collection tube is 5mL test tube.
Fig. 7 is an extraction element being furnished with waste liquid tank, and this waste liquid tank is for collecting waste liquid.
Fig. 8 A is a heating group of not assembling any Heating element.Fig. 8 B is a heating group being equipped with Heating element.
Fig. 9 is an extraction element being furnished with vacuum group.
Figure 10 is an extraction element comprising four functional group and the O type ring between functional group.
Embodiment
embodiment 1
nucleic acid-extracting apparatus
In one embodiment, nucleic acid-extracting apparatus as shown in Figure 1, forms by with lower part:
I) extract pylon (10), comprise one or more perforate mouth, for supporting one or more extraction column (11), described extraction column contains sample, reagent or its combination;
Ii) collection group (20), comprises one or more grooves at an upper portion thereof, is used for supporting one or more collection tube (21), and described collection tube receives liquid from extraction column or the sample that contains containing nucleic acid;
Iii) heating group (30), comprises one or more heating chamber (31), for controlling the temperature of collection tube; The bottom of described extraction pylon is placed on the groove of described collection group, and the bottom of described collection group comprises one or more coupling device (22), is used for being connected with the heating chamber of described heating group.
In one embodiment, extract pylon (10) to combine with the top of collection group (20), wherein said collection group (20) and then combined by the top of coupling device (22) with heating group (30).Multiple groove (23) is contained in the upper end of collection group (20), for supporting collection tube (21).The lower end of extracting pylon (10) is placed on the upper end of collection group (20), thus makes the collection tube (21) be placed in the groove (23) of collection group (20) combine with extraction column (11).In one embodiment, the lower end of collection group (20) comprises multiple coupling device (22), can insert in the heating chamber (31) of the upper end being in heating group (30).
In one embodiment, nucleic acid-extracting apparatus as shown in Figure 4, extraction pylon (10) of the present invention also can comprise one or more extraction column Heating element (15), for the sample in extraction column (11) provides or produces heat.In one embodiment, extract pylon (10) and comprise one or more slot (16) be placed between hollow tube chamber (12) side or tube chamber (12) ranks, slot (16) is for receiving extraction column Heating element (15).In extraction pylon (10) shown in Fig. 4, the hollow tube chamber (12) often arranged is sandwiched between two slots (16).In one embodiment, extraction column Heating element (15) is embedded in the one or more slots (16) extracted in pylon (10).
In one embodiment, nucleic acid-extracting apparatus as shown in Figure 7 also comprises a waste liquid tank (40), waste liquid tank (40) is placed on and extracts between pylon (10) and collection group (20), for collecting from extraction column (11) waste liquid out or the material (Fig. 7) thrown aside.In one embodiment, waste liquid tank (40) is a removal waste fluid bucket (41).Waste liquid barrel (41) can be placed in the groove (23) of collection group (20) or be placed in the collection tube (21) in described groove (23).Difference can be coordinated to need the waste liquid barrel (41) of design and use different diameter and size.
In one embodiment, heating group (30) as shown in Figure 8 A can with one allot outward thermal with the use of, give sample to provide heating power.In one embodiment, outer allocation hot charging is set to guiding device or heating board.The flat bottom of heating group (30) is directly placed on the top of described electro-heat equipment.Heating power transfers to the bottom of heating group by described electro-heat equipment, finally arrives collection tube (21), and wherein collection tube (21) is inserted in heating room (31) by coupling device (22).In another embodiment, heating group (30) also comprises one or more Heating element (32) being arranged on heating group (30) bottom, can provide heating power when not connecting outer allocation thermal.As shown in Figure 8 B, individual other Heating element (32) is arranged on the below of the heating room (31) in heating group (30), to provide heating power directly to sample.Heating element (32) also comprises the secondary Heating element be placed near collection tube.Heating element (32) can be any Heating element, such as resistance heating element, includes but not limited to metal Fa Re Yuan Jian ﹑ ceramic heating element and synthesis Heating element.In one embodiment, Heating element (32) is thermoelectric cooling module (Thermoelectric Cooling, TEC), printed circuit board (PCB), flexible circuit board or ceramic heating.
In one embodiment, as shown in Figure 9, device of the present invention is by connecting extraction pylon (10) of the present invention, collection group (20) and heating group (30) and make, wherein vacuum group (50) is directly placed on the bottom of heating group (30).In one embodiment, heating group (30) is embedded in the opening (51) of vacuum group (50), described vacuum group opening (51) is defined by the upper end of vacuum group (50) outer wall, and with the shape complementarity of heating group.
In one embodiment, as shown in Figure 10, device of the present invention is first by extracting pylon O type ring and will extract pylon (10) and collection group (20) combining.Extract pylon O type ring (18) and there is retractility and elasticity, can be centered around on the outward flange of extraction pylon (10) and collection group (20).The edge at the edge and collection group (20) top that extract pylon (10) bottom can comprise groove further to add stressed joint extraction column frame O type ring (18).Then, the coupling device (22) of collection group (20) is directly connected with the heating room (31) of heating group (30), and the edge at heating room (31) top contains a groove with compact siro spinning technology heating group O type ring (33).The planar base of heating group (30) is connected with the vacuum openings in vacuum group (50) by vacuum O type ring (52) subsequently.Vacuum group O type ring (52) is centered around on the outward flange of heating group (30) and vacuum group (50).
embodiment 2
nucleic acid extraction
This example demonstrates the step extracting nucleic acid from sample.
The present invention can use as Xi Di Ye ﹑ elutriant and other reagent Xiang Knot for nucleic acid extraction close with any available Zhu ﹑ Ping ﹑ reagent.
Use nucleic acid-extracting apparatus as shown in Figure 1, flow process through the following steps, can extract nucleic acid from sample:
1. get the appropriate samples containing required nucleic acid;
2. anticipate sample with suitable cracking agent or device, thus obtain the non-Pureization extract containing required nucleic acid;
3. the sample containing required nucleic acid or non-Pureization extract and suitable reagent are joined in the extraction column (11) extracted on pylon (10), wherein extraction column (11) is furnished with suitable film or filter membrane;
4. collection is from the material of extraction column discharge to collection tube (21);
5. remove from collection tube (21) material collected;
6. add washings or suitable reagent in extraction column (11), clean film or filter membrane with one or many;
7. the wash-out nucleic acid from extraction column (11) with elutriant or suitable elution reagent;
8. collect nucleic acid with collection tube (21).
One of them example of following As extraction step:
Step described above can be combined with sucking force generator or flow hybridization device by device of the present invention and implement, and thus Shi Ye Body quickly flows across extraction element.Step described above also can by device of the present invention or Zhuan Zhi Knot of the present invention close external device (ED) (as guiding device or hot-plate) carry out under the intensification that maintains.In one embodiment, if do not need to provide heating power, the process of whole Ge nucleic acid extraction can complete in 5-7 minute.
In one embodiment, the nucleic acid prepared by the present invention can join any Mo ﹑ Guan ﹑ Ping ﹑ plate or suitable material or container Inner ， Yi Jin capable next step Office reason as PCR Kuo Zeng ﹑ Za Jiao ﹑ Biao Ji ﹑ restriction enzyme digestion and DNA sequencing.In one embodiment, the capable laboratory of nucleic acid Yi Jin that the Measuring Try the present invention such as different Shi Zhi ﹑ reagent Tiao ﹑ Shi Ji He ﹑ methods can be used to prepare or Lin Chuan Measuring Try.
In one embodiment, nucleic acid prepared by the present invention can be added to and be placed on collection tube (21) in collection group (20) or other have suitable size and in the collection tube that manufactures with suitable material, the amplification when heating group (30) provides heating power after the Hang Sui of Jin or mark.
Claims (19)
1. for extracting a nucleic acid-extracting apparatus for nucleic acid, it is characterized in that, forming by with lower part:
I) extract pylon, comprise one or more perforate mouth, for supporting one or more extraction column, described extraction column contains sample, reagent or its combination;
Ii) collection group, comprises one or more grooves at an upper portion thereof, is used for supporting one or more collection tube, and described collection tube receives liquid from extraction column or the sample that contains containing nucleic acid;
Iii) heating group, comprises one or more heating chamber, for controlling the temperature of collection tube;
The bottom of described extraction pylon is placed on the groove of described collection group, and the bottom of described collection group comprises one or more coupling device, is used for being connected with the heating chamber of described heating group.
2. nucleic acid-extracting apparatus according to claim 1, is characterized in that, also comprises one and covers the lid extracting pylon top.
3. nucleic acid-extracting apparatus according to claim 1, it is characterized in that, between extraction pylon and collection group, also have a waste liquid tank, for collecting from extraction column waste liquid out, described waste liquid tank comprises one or more waste liquid barrel to connect the groove in described collection group.
4. nucleic acid-extracting apparatus according to claim 1, is characterized in that, also comprises one or more O type ring, and described O type ring is in following one or more position:
I) extract between pylon and collection group, and
Ii) between the coupling device of collection group and heating group.
5. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said extraction pylon is on perforate mouth side or contain one or more slots between described perforate mouth, and each described slot embeds extraction column Heating element.
6. nucleic acid-extracting apparatus according to claim 5, is characterized in that, wherein said extraction column Heating element is a kind of resistance heating element, and described resistance heating element is selected from metal Heating element, ceramic heating element or synthesis Heating element.
7. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said extraction column is equipped with an adapter, and for containing sample or the reagent of more volume, described adapter connects with the opening of described extraction column.
8. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said collection group is a waste collection Zu ﹑ sample collection Zu ﹑ wash-out group or sample recovery group.
9. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said heating group comprises one or more Heating element, and described Heating element is arranged on the below of the heating chamber of heating group Inner.
10. nucleic acid-extracting apparatus according to claim 9, is characterized in that, wherein said Heating element is selected from: metal Fa Re Yuan Jian ﹑ ceramic heating element and synthesis Heating element.
11. nucleic acid-extracting apparatus according to claim 1, it is characterized in that, also comprise a vacuum group combined with suction generating device, described vacuum group comprises the opening that has porous head, described hole head allows air Tong Over wherein, and the bottom of wherein heating group is embedded in the opening of described vacuum group.
12. nucleic acid-extracting apparatus according to claim 11, is characterized in that, also comprise one or more O type ring, and described O type ring is in following one or more position:
I) extract between pylon and collection group;
Ii) between the coupling device of collection group and heating group; And
Iii) between heating group and vacuum group.
13. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said extraction column is embedded in the filter membrane or film that are applicable to extract nucleic acid.
14. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said Qi Group of Yang Pin ﹑ reagent Huo closes and arrives collection tube or waste liquid tank by extraction column.
15. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said nucleic acid extracts from biological sample.
16. nucleic acid-extracting apparatus according to claim 15, is characterized in that, wherein said biological sample is Xue Ye ﹑ Xue Qing ﹑ Xue Jiang ﹑ Tuo Ye ﹑ Tan ﹑ Jing Ye ﹑ lymph liquid or other body fluid.
17. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said device and water conservancy diversion hybridize Zhuan Zhi ﹑ reverse spot flow hybridization device or any can carry out flow hybridization device or instrument be combined.
18. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said device is combined with electro-heat equipment or hot-plate.
19. nucleic acid-extracting apparatus according to claim 1, is characterized in that, wherein said device is combined with the system of automatization or mechanize.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420507166.8U CN204298379U (en) | 2014-09-04 | 2014-09-04 | The portable extraction element of rapid extraction nucleic acid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420507166.8U CN204298379U (en) | 2014-09-04 | 2014-09-04 | The portable extraction element of rapid extraction nucleic acid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110170182A (en) * | 2019-06-12 | 2019-08-27 | 江苏睿玻生物科技有限公司 | Negative pressure purification devices and negative pressure purification process |
| CN110193216A (en) * | 2019-06-12 | 2019-09-03 | 江苏睿玻生物科技有限公司 | Negative pressure collecting device and negative pressure collection method |
| TWI792671B (en) * | 2021-11-11 | 2023-02-11 | 台達電子工業股份有限公司 | Aerosol filter and nucleic acid extraction cassette |
-
2014
- 2014-09-04 CN CN201420507166.8U patent/CN204298379U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110170182A (en) * | 2019-06-12 | 2019-08-27 | 江苏睿玻生物科技有限公司 | Negative pressure purification devices and negative pressure purification process |
| CN110193216A (en) * | 2019-06-12 | 2019-09-03 | 江苏睿玻生物科技有限公司 | Negative pressure collecting device and negative pressure collection method |
| CN110170182B (en) * | 2019-06-12 | 2021-08-10 | 江苏睿玻生物科技有限公司 | Negative pressure purification device and negative pressure purification method |
| TWI792671B (en) * | 2021-11-11 | 2023-02-11 | 台達電子工業股份有限公司 | Aerosol filter and nucleic acid extraction cassette |
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