CN204272864U - A kind of Enteromorpha microcosmic brood body incubator - Google Patents

A kind of Enteromorpha microcosmic brood body incubator Download PDF

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Publication number
CN204272864U
CN204272864U CN201420666407.3U CN201420666407U CN204272864U CN 204272864 U CN204272864 U CN 204272864U CN 201420666407 U CN201420666407 U CN 201420666407U CN 204272864 U CN204272864 U CN 204272864U
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cup
bowl cover
enteromorpha
brood body
microcosmic
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CN201420666407.3U
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徐文婷
黄艳
韩军军
何培民
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Shanghai Maritime University
Shanghai Ocean University
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Shanghai Maritime University
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Abstract

The utility model relates to a kind of incubator, particularly a kind of use for laboratory Enteromorpha microcosmic brood body incubator, and this incubator is made up of cup, bowl cover, and bowl cover and cup are flexibly connected by screw thread; Described cup is up big and down small bowl structure, and described bowl cover is provided with ventilation closing lid, and the bottom of this ventilation closing lid and bowl cover one are synthesized, and the top of this ventilation closing lid and both sides are that the circular arc that can upwardly open is renovated; Described bowl cover is provided with the semi-circle shaped transparent thin slice identical with the radius of bowl cover, and the bottom of this thin slice is fixed on the centerline of bowl cover, and remainder can upwardly open; Described bottom of cup is shelved with rectangle Enteromorpha brood body slide glass, Enteromorpha microcosmic brood body adheres on this slide glass, be convenient to direct gripping, neither pollute other brood body, also ensure that the integrality of brood body attachment base portion simultaneously, and to use and very convenient to carry, energy clear view is to the germinating of the very high Enteromorpha spore count of density.

Description

A kind of Enteromorpha microcosmic brood body incubator
Technical field
The utility model relates to a kind of incubator, particularly a kind of use for laboratory Enteromorpha microcosmic brood body incubator.
Background technology
Green tide refers to the phenomenon (Hiraoka et al., 2003c) being caused coastwise being formed a slice greenbelt by green alga hyper-proliferatives such as Ulva, Enteromorpha, bristle Trentepohlia and Cladophoras.Worldwide, from North America, Europe, Asia be coastal to Oceania, all constantly has green tide to occur in recent years, cause increasingly serious negative effect to coastal ecology environment and Tourism Economy.2007-2014, China's Huanghai Sea continuous 8 years green damp disasters of outburst, impact scope is wide, and the duration is long, and biomass is huge.Wherein the maximum area that relates to reaches 60,000 square kilometres (2010), and maximum coverage area reaches 530 square kilometres (2011).Through qualification, Enteromorpha algae is the dominant species of green tide outburst.This shows, the outburst of green tide is relevant to the history of life of Enteromorpha, is also very necessary to Enteromorpha studies of the life histories.
The cultivation of current experiment desk research microcosmic brood body and observe main based on culture dish, beaker, conical flask be auxiliary.Culture dish is mainly used for observing spore release situation, Enteromorpha blade segment is cultivated in culture dish, adds VSE culture fluid, and the methods such as illumination stimulate impels it to diffuse spore, then is observed the situation of diffusing of spore by microscope, flagellum radical etc.But because culture dish itself is very short and small, the Enteromorpha total amount that can carry is very limited, is unfavorable for the cultivation of amount reproduction body.Simultaneously because the character of Enteromorpha cell wall itself is namely more at the hop count cut, when concentration is higher, cell wall can discharge inhibitory substance and hinder diffusing of spore, and what therefore culture dish was unfavorable for when demand trial of strength is many observation spore diffuses situation.Beaker is generally used for cultivating Enteromorpha microcosmic brood body, at the large beaker upper cover last layer preservative film of about 300 milliliters, to prevent the evaporation of seawater, may pour in beaker with the water sample of Enteromorpha microcosmic brood body and cultivate, and after Enteromorpha seedling grows, it observed and count.This training method compensate for the deficiency on capacity than culture dish, but is but not easy to observe.Beaker is excessive, with tweezers pinch get time also inconvenient, and preservative film can not reuse, and can not carry out its air-charging incubation to brood body.Preservative film rapid wear simultaneously, once damaged assorted algae will pollute brood body.In addition, be also unfavorable for that repeatedly carrying out sampling observes.Part Experiment room also utilizes conical flask to cultivate microcosmic brood body, but sample tap is too little, sampling and Counts and inconvenience thereof.
This patent provides the incubator of a kind of Enteromorpha microcosmic brood body, is used for the Early observation of the Reproductive development of Enteromorpha and research.
Summary of the invention
The technical problems to be solved in the utility model is to provide Enteromorpha microcosmic brood body incubator, and this incubator good permeability is easy to use, and ensure that the integrality of Enteromorpha brood body attachment base portion, avoids the pollution of other brood bodies.
For solving the problems of the technologies described above, realize by following technical scheme,
A kind of Enteromorpha microcosmic brood body incubator, it is characterized in that: this incubator is made up of cup, bowl cover, bowl cover and cup are flexibly connected by screw thread;
Described cup is up big and down small bowl structure, and the vertical range of rim of a cup top to rim of a cup of cup is 8-11cm, and the upper end rim of a cup diameter of cup is 8.5-10cm, and lower end rim of a cup is 7-8.5cm;
The inner side of described bowl cover is provided with screw thread, matches with the screw thread in the rim of a cup outer upper end portion of cup; Described bowl cover is provided with ventilation closing lid, and the bottom of this ventilation closing lid and bowl cover one are synthesized, and the top of this ventilation closing lid 4 and both sides are that the circular arc that can upwardly open is renovated;
Described bowl cover is provided with the semi-circle shaped transparent thin slice identical with the radius of bowl cover, and the bottom of this thin slice is fixed on the centerline of bowl cover, and remainder can upwardly open;
Described bottom of cup is shelved with rectangle Enteromorpha brood body slide glass.
Wherein, described cup and bowl cover material are lucite.Light transmission is good, durable, non-friable.
Wherein, the thickness of described thin slice is 0.03-0.07mm.Phototaxis experiment can be carried out by under ironed for light-shielding sheet, and suitable sheet thickness, be conducive to chip sorption on bowl cover.
The material of described Enteromorpha brood body slide glass is glass, and the length of this Enteromorpha brood body slide glass is 1-3cm, and wide is 1-3cm, and thick is 0.1-0.5mm.Enteromorpha brood body anchors on the glass slide of bottom, and when observation, direct clamping parts slide is placed on slide, neither pollutes other brood body, also ensure that the integrality of brood body attachment base portion simultaneously.
The lid limit of described bowl cover is provided with semiorbicular slot with thin slice corresponding section, when being convenient to carry out phototaxis experiment, light-shielding sheet is rolled into semicircle shape, poor from top to bottom in slot, the half of cup is all carried out shading treatment.
Compared with prior art, the utility model has the advantages that: 1, described Enteromorpha microcosmic brood body incubator is up big and down small bowl structure, is convenient to taking of algae, and can clear view to the germinating of the very high Enteromorpha spore count of density.2, the bowl cover difficult drop-off be threaded described in, ensure that mobile incubator formula, the culture fluid in cup can not be splashed away.3, bowl cover is provided with that ventilation closing lid is convenient carries out supplementing of nutritive salt and inflation to brood body, and the used time can not close, and makes microcosmic brood body by the pollution of assorted algae, and easy to use.4, described Enteromorpha microcosmic brood body is attached on the glass slide of bottom, and when observation, direct clamping parts slide is placed on slide, neither pollutes other brood body, also ensure that the integrality of brood body attachment base portion, and it is very convenient to take simultaneously.
Accompanying drawing explanation
Fig. 1 is Enteromorpha microcosmic brood body incubator structural representation, and wherein ventilation closing lid and thin slice are opening.
Fig. 2 Enteromorpha microcosmic brood body incubator structural representation, wherein ventilation closing lid and thin slice are closed condition.Fig. 3 is the vertical view of cup.
Fig. 4 is the enlarged diagram of bowl cover slot.
In figure: 1. cup, 2. bowl cover, 3. Enteromorpha brood body slide glass, 4. ventilate closing lid, 5. thin slice, 6. slot.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
As shown in Figure 1, Figure 2, shown in Fig. 3 and Fig. 4, a kind of Enteromorpha microcosmic brood body incubator, it is characterized in that: this incubator is made up of cup 1, bowl cover 2, bowl cover 2 and cup 1 are flexibly connected by screw thread; Described bowl cover cup is lucite material.
Described cup 1 is up big and down small bowl structure, and the vertical range of rim of a cup top to rim of a cup of cup 1 is 8cm, and the upper end rim of a cup diameter of cup 1 is 10cm, and lower end rim of a cup is 7cm;
The inner side of described bowl cover 2 is provided with screw thread, matches with the screw thread in the rim of a cup outer upper end portion of cup 1; Described bowl cover 2 is provided with ventilation closing lid 4, and the bottom of this ventilation closing lid 4 and bowl cover 2 one are synthesized, and the top of this ventilation closing lid 4 and both sides are that the circular arc that can upwardly open is renovated;
Described bowl cover 2 is provided with the semi-circle shaped transparent thin slice 5 identical with the radius of bowl cover 2, and the bottom of this thin slice 5 is fixed on the centerline of bowl cover, and remainder can upwardly open; The thickness of this thin slice is 0.05mm.The lid limit of described bowl cover 1 is provided with semiorbicular slot 6 with thin slice 5 corresponding section, and the width of this slot 6 is advisable for just in time blocking light-shielding sheet.
Be shelved with rectangle Enteromorpha brood body slide glass 3 bottom described cup 1, the material of described Enteromorpha brood body slide glass 3 is glass, and the length of this Enteromorpha brood body slide glass 3 is 2cm, and wide is 2cm, and thick is 0.2mm.
During use, open bowl cover, the water sample containing Enteromorpha microcosmic brood body is put into, build lid, open ventilation closing lid 4, inflating catheter is stretched in cup by ventilation closing lid 4 and carries out air-charging incubation, the aperture of this ventilation closing lid 4 is 0.5-1cm, is then put in constant incubator by this device.The tapping Additional nutrient solution of conduit at ventilation closing lid 4 is extracted after several days.Analysis of accounts is carried out or gripping Enteromorpha individual plant carries out Molecular Identification work after seedling grows up to.
More than show and describe general principle of the present utility model and principal character and advantage thereof.The technology personage of the industry should understand; the utility model is not by the restriction of above-mentioned implementing regulations; describe in above-mentioned implementing regulations and specification just for illustration of principle of the present utility model; under the prerequisite not departing from the utility model principle and scope; the utility model also can have various changes and modifications, and these changes and improvements all belong within the scope of claimed the utility model.
The claimed scope of the utility model defines with appending claims and other equivalent.

Claims (5)

1. an Enteromorpha microcosmic brood body incubator, is characterized in that: this incubator is made up of cup (1), bowl cover (2), and bowl cover (2) and cup (1) are flexibly connected by screw thread;
Described cup (1) is up big and down small bowl structure, and the vertical range of rim of a cup top to rim of a cup of cup (1) is 8-11cm, and the upper end rim of a cup diameter of cup (1) is 8.5-10cm, and lower end rim of a cup is 7-8.5cm;
The inner side of described bowl cover (2) is provided with screw thread, matches with the screw thread in the rim of a cup outer upper end portion of cup (1); Described bowl cover (2) is provided with ventilation closing lid (4), the bottom of this ventilation closing lid (4) and bowl cover (2) one are synthesized, and the top of this ventilation closing lid (4) and both sides are that the circular arc that can upwardly open is renovated;
Described bowl cover (2) is provided with the semi-circle shaped transparent thin slice (5) identical with the radius of bowl cover (2), and the bottom of this thin slice (5) is fixed on the centerline of bowl cover, and remainder can upwardly open;
Described cup (1) bottom is shelved with rectangle Enteromorpha brood body slide glass (3).
2. Enteromorpha microcosmic brood body incubator according to claim 1, is characterized in that: described cup (1) and bowl cover (2) material are lucite.
3. Enteromorpha microcosmic brood body incubator according to claim 1, is characterized in that: the thickness of described thin slice (5) is 0.03-0.07mm.
4. Enteromorpha microcosmic brood body incubator according to claim 1, it is characterized in that: the material of described Enteromorpha brood body slide glass (3) is glass, the length of this Enteromorpha brood body slide glass (3) is 1-3cm, and wide is 1-3cm, and thick is 0.1-0.5mm.
5. Enteromorpha microcosmic brood body incubator according to claim 1, is characterized in that: the lid limit of described bowl cover (1) is provided with semiorbicular slot (6) with thin slice (5) corresponding section.
CN201420666407.3U 2014-11-10 2014-11-10 A kind of Enteromorpha microcosmic brood body incubator Active CN204272864U (en)

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CN201420666407.3U CN204272864U (en) 2014-11-10 2014-11-10 A kind of Enteromorpha microcosmic brood body incubator

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108801675A (en) * 2018-08-07 2018-11-13 中国科学院南京地理与湖泊研究所 The collection device and method in a kind of deposit surface sedimentation water plant brood body library

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108801675A (en) * 2018-08-07 2018-11-13 中国科学院南京地理与湖泊研究所 The collection device and method in a kind of deposit surface sedimentation water plant brood body library
CN108801675B (en) * 2018-08-07 2024-05-28 中国科学院南京地理与湖泊研究所 Device and method for collecting sediment surface subsided aquatic plant propagule library

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