CN204185412U - A kind of separating extraction device for mycotoxins yolk antibody - Google Patents
A kind of separating extraction device for mycotoxins yolk antibody Download PDFInfo
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- CN204185412U CN204185412U CN201420563110.4U CN201420563110U CN204185412U CN 204185412 U CN204185412 U CN 204185412U CN 201420563110 U CN201420563110 U CN 201420563110U CN 204185412 U CN204185412 U CN 204185412U
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- yolk
- valve
- yolk antibody
- mycotoxins
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Abstract
The utility model belongs to yolk mycotoxins instrument for extracting applied technical field, specifically discloses a kind of separating extraction device for mycotoxins yolk antibody, is made up of yolk separator assembly, yolk antibody lixiviate assembly and yolk antibody purifying assembly; Described yolk separator assembly comprises yolk suction pipe, the first valve and vacuum pump; Described yolk antibody lixiviate assembly pot for solvent extraction, stirring rotator and magnetic stirring apparatus; Described yolk antibody purifying assembly comprises the second valve, filter, the first constant flow pump, the 3rd valve, the 4th valve, the second constant flow pump and eumycetin antigen affinity column.The beneficial effects of the utility model are: 1, one-piece construction is simple, is convenient to clean and maintenance; 2, on the one hand integrally combined institute floor space little, reduce factory building cost and drop into, omnidistancely on the other hand adopt that Artificial Control power operation completes, working efficiency is high; 3, yolk antibody carries out purifying operation without the need to adopting independent purification system, extraction purification simple to operate and reduce equipment purchase drop into.
Description
Technical field
The utility model relates to yolk mycotoxins instrument for extracting technical field, is specifically related to a kind of separating extraction device for mycotoxins yolk antibody.
Background technology
The pollution problem of mycotoxins in grain and feed is more and more serious, become significant problem very important in modern agricultural production and grain security, and antibody is as the important instrument of mycotoxins prevention and control, be widely used in mycotoxins to detect and prevention area, because yolk antibody is tired height, output is large, good stability, comparatively conventional animal antibody has a clear superiority in, and is just more and more widely adopted.
At present, mycotoxins yolk antibody acquisition technique is by mycotoxins artificial antigen Immune Laying Hens, the antibody for mycotoxins can be produced in the yolk of egg, by collecting the egg after immunity, be separated yolk, extract yolk antibody, but because yolk antibody is present in yolk, need to isolate yolk, remove egg white, and obtain required yolk antibody by purifying, existing technology many employings Yolk separator hand yolk, or heavy-duty machines is separated yolk, because hand speed is slow, machine is separated yolk and is used for industry or food-processing, be separated rough, residual egg white is many, and footprint area is large, also exist after yolk is separated, yolk antibody extraction purification also needs separately to carry out purifying operation by independent extraction system, not easily technical sophistication and input cost is large simultaneously.
Therefore, based on the problems referred to above, the utility model provides a kind of sharp separation extraction element being applicable to mycotoxins yolk antibody.
Utility model content
Utility model object: a kind of separating extraction device for mycotoxins yolk antibody of the purpose of this utility model, solve existing mycotoxins and extract that its efficiency of equipment of adopting of operation is low, cost is high and the problem that loss is large, improve mycotoxins and extract operating efficiency, reduce costs.
Technical scheme: be made up of yolk separator assembly, yolk antibody lixiviate assembly and yolk antibody purifying assembly; Described yolk separator assembly comprises yolk suction pipe, the first valve and vacuum pump; Described yolk antibody lixiviate assembly pot for solvent extraction, stirring rotator and magnetic stirring apparatus; Described yolk antibody purifying assembly comprises the second valve, filter, the first constant flow pump, the 3rd valve, the 4th valve, the second constant flow pump and eumycetin antigen affinity column; Described yolk suction pipe one end is connected with pot for solvent extraction, and the other end is connected with broken egg collection device; Described vacuum pump is connected with pot for solvent extraction by pipe connecting, and wherein, the first valve installation is on pipe connecting; Described magnetic stirring apparatus is arranged on pot for solvent extraction tank base, wherein, magnetic stirring apparatus arranges stirring rotator; Described filter, the first constant flow pump, the second constant flow pump are connected by pipeline successively with eumycetin antigen affinity column, and wherein, pipeline one end connects pot for solvent extraction, and the other end connects elutriant storing device; Described second valve installation is in the middle of pot for solvent extraction and filter; Described 3rd valve, the 4th valve installation in the middle of the first constant flow pump, the second constant flow pump, and are positioned at rhzomorph antigen affinity column both sides.
The technical program, described yolk suction pipe employing diameter is the PVC plastic flexible pipe of 2.5cm, and length is 1.5m.
The technical program, the aperture of described filter is 0.5 micron.
Compared with prior art, the beneficial effect of a kind of separating extraction device for mycotoxins yolk antibody of the present utility model is: 1, one-piece construction is simple, is convenient to clean and maintenance; 2, on the one hand integrally combined institute floor space little, reduce factory building cost and drop into, omnidistancely on the other hand adopt that Artificial Control power operation completes, working efficiency is high; 3, yolk antibody carries out purifying operation without the need to adopting independent purification system, extraction purification simple to operate and reduce equipment purchase drop into.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of separating extraction device for mycotoxins yolk antibody of the utility model;
Wherein in figure, sequence number is as follows: 1-yolk suction pipe, 2-first valve, 3-vacuum pump, 4-pot for solvent extraction, 5-stirring rotator, 6-magnetic stirring apparatus, 7-second valve, 8-filter, 9-first constant flow pump, 10-the 3rd valve, 11-the 4th valve, 12-second constant flow pump, 13-eumycetin antigen affinity column.
Embodiment
Below in conjunction with the drawings and specific embodiments, illustrate the utility model further.
A kind of separating extraction device for mycotoxins yolk antibody as shown in Figure 1, is made up of yolk separator assembly, yolk antibody lixiviate assembly and yolk antibody purifying assembly; Described yolk separator assembly comprises yolk suction pipe 1, first valve 2 and vacuum pump 3; Yolk antibody lixiviate assembly pot for solvent extraction 4, stirring rotator 5 and magnetic stirring apparatus 6; Yolk antibody purifying assembly comprises the second valve 7, filter 8, first constant flow pump 9, the 3rd valve 10, the 4th valve 11, second constant flow pump 12 and eumycetin antigen affinity column 13; Yolk suction pipe 1 one end is connected with pot for solvent extraction 4, and the other end is connected with broken egg collection device; Vacuum pump 3 is connected with pot for solvent extraction 4 by pipe connecting, and wherein, the first valve 2 is arranged on pipe connecting; Magnetic stirring apparatus 6 is arranged on pot for solvent extraction 4 tank base, wherein, magnetic stirring apparatus 6 is arranged stirring rotator 5; Filter 8, first constant flow pump 9, second constant flow pump 12 is connected by pipeline successively with eumycetin antigen affinity column 13, and wherein, pipeline one end connects pot for solvent extraction 4, and the other end connects elutriant storing device; Second valve installation is in the middle of pot for solvent extraction 4 and filter 8; 3rd valve 10, the 4th valve 11 are arranged in the middle of the first constant flow pump 9, second constant flow pump 12, and are positioned at rhzomorph antigen affinity column 13 both sides.
Preferably, yolk suction pipe 1 adopts diameter to be the PVC plastic flexible pipe of 2.5cm, and length is 1.5m, and cost is low and adsorption effect is excellent; The aperture of filter 8 is 0.5 micron, is filtered by macrobead fast and removes; Wherein, pot for solvent extraction 4 volume is 50L.
Embodiment
Further as shown in Figure 1, after immunity egg cracks eggshell, yolk can pick up by the suction that yolk suction pipe 1 is produced by vacuum pump 3, egg white can not be sucked away (integrity of yolk vitelline membrane and yolk suction pipe 1 caliber coincide) by the effect due to gravity and resistance during yolk suction pipe 1 device mouth, pot for solvent extraction 4 can be entered by negative-pressure sucking thus thoroughly be separated with egg white, 30L water can be put in advance in pot for solvent extraction 4, stirring rotator 4 drives not stall to move by magnetic stirring apparatus 6, after 500 yolk enter, continue stirring and leaching 4 hours, yolk antibody can be dissolved in vat liquor after stirring and leaching, close the first valve 2, open the first constant flow pump 9, second valve 7 and the 3rd valve 10, vat liquor can filter removal macrobead through filter 8 by sluggish flow, enter mycotoxins antigen affinity column 13, mycotoxins yolk antibody can with mycotoxins antigen affinity column 13 specific binding, other foreign proteins uncombined can flow out, required mycotoxins yolk antibody is combined on mycotoxins antigen affinity column 13, after vat liquor completion of the sample, open the 4th valve 11 and the second constant flow pump 12, 3rd valve 10 is closed, start wash-out yolk antibody, mycotoxins yolk antibody can come off from mycotoxins antigen affinity column 13 under elutriant effect, thus complete the separation and purification of whole mycotoxins antibody, collect.
The whole separation and purification device of this structure is simple to operate, and a separation and purification speed fast device can complete the separation and purification of 500 yolk the same day and installation cost is low, and convenient operation uses.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the utility model principle; can also make some improvement, these improvement also should be considered as protection domain of the present utility model.
Claims (3)
1., for a separating extraction device for mycotoxins yolk antibody, it is characterized in that: by yolk separator assembly,
Yolk antibody lixiviate assembly and yolk antibody purifying assembly composition; Described yolk separator assembly comprises yolk suction pipe (1), the first valve (2) and vacuum pump (3); Described yolk antibody lixiviate assembly pot for solvent extraction (4), stirring rotator (5) and magnetic stirring apparatus (6); Described yolk antibody purifying assembly comprises the second valve (7), filter (8), the first constant flow pump (9), the 3rd valve (10), the 4th valve (11), the second constant flow pump (12) and eumycetin antigen affinity column (13); Described yolk suction pipe (1) one end is connected with pot for solvent extraction (4), and the other end is connected with broken egg collection device; Described vacuum pump (3) is connected with pot for solvent extraction (4) by pipe connecting, and wherein, the first valve (2) is arranged on pipe connecting; Described magnetic stirring apparatus (6) is arranged on pot for solvent extraction (4) tank base, wherein, magnetic stirring apparatus (6) is arranged stirring rotator (5); Described filter (8), the first constant flow pump (9), the second constant flow pump (12) are connected by pipeline successively with eumycetin antigen affinity column (13), and wherein, pipeline one end connects pot for solvent extraction (4), and the other end connects elutriant storing device; Described second valve installation is in the middle of pot for solvent extraction (4) and filter (8); Described 3rd valve (10), the 4th valve (11) are arranged in the middle of the first constant flow pump (9), the second constant flow pump (12), and are positioned at rhzomorph antigen affinity column (13) both sides.
2. a kind of separating extraction device for mycotoxins yolk antibody according to claim 1, is characterized in that: described yolk suction pipe (1) employing diameter is the PVC plastic flexible pipe of 2.5cm, and length is 1.5m.
3. a kind of separating extraction device for mycotoxins yolk antibody according to claim 1, is characterized in that: the aperture of described filter (8) is 0.5 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420563110.4U CN204185412U (en) | 2014-09-28 | 2014-09-28 | A kind of separating extraction device for mycotoxins yolk antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420563110.4U CN204185412U (en) | 2014-09-28 | 2014-09-28 | A kind of separating extraction device for mycotoxins yolk antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204185412U true CN204185412U (en) | 2015-03-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420563110.4U Expired - Fee Related CN204185412U (en) | 2014-09-28 | 2014-09-28 | A kind of separating extraction device for mycotoxins yolk antibody |
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| CN (1) | CN204185412U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115925916A (en) * | 2023-01-05 | 2023-04-07 | 恒诺优(天津)生物技术有限公司 | Separation and extraction device for mycotoxin egg yolk antibody |
-
2014
- 2014-09-28 CN CN201420563110.4U patent/CN204185412U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115925916A (en) * | 2023-01-05 | 2023-04-07 | 恒诺优(天津)生物技术有限公司 | Separation and extraction device for mycotoxin egg yolk antibody |
| CN115925916B (en) * | 2023-01-05 | 2024-01-30 | 恒诺优(天津)生物技术有限公司 | Separation and extraction device for mycotoxin egg yolk antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20150928 |
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| EXPY | Termination of patent right or utility model |