CN204008469U - Fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment - Google Patents
Fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment Download PDFInfo
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- CN204008469U CN204008469U CN201420415758.7U CN201420415758U CN204008469U CN 204008469 U CN204008469 U CN 204008469U CN 201420415758 U CN201420415758 U CN 201420415758U CN 204008469 U CN204008469 U CN 204008469U
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Abstract
The utility model discloses the fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment, comprise generating device of laser, fixed mount, fluorospectrophotometer, lucifuge plate, laser emission probe, cuvette, wherein: fluorospectrophotometer comprises Ear Mucosa Treated by He Ne Laser Irradiation module and signal acquisition module; Ear Mucosa Treated by He Ne Laser Irradiation module upper surface has circular open, and inner middle part is provided with the accommodating cuvette of room; Signal acquisition module comprises detecting device and signal gathering unit, and detecting device is located at room place, and the spectral signal sending for detection of the fluorescent marker in cuvette is also passed to signal gathering unit; The laser probe of generating device of laser enters fluorospectrophotometer by circular open and is positioned at cuvette top; Fixed mount is for fixed laser probe; Ear Mucosa Treated by He Ne Laser Irradiation module top is provided with two blocks of middle lucifuge plates with semicircle groove on one side, and two semicircle grooves are combined corresponding to circular open and cover and at Ear Mucosa Treated by He Ne Laser Irradiation module upper surface, circular open is sealed completely and fixed laser probe.
Description
Technical field
The utility model relates to laser and optical dynamic therapy experimental provision field, in particular to the fluorescence monitoring device in the experiment of a kind of laser and optical dynamic therapy.
Background technology
Optical dynamic therapy (Photodynamic therapy) is a kind of new disease treatment means taking the interaction of light, photosensitizer and oxygen as basis, that a species specific photosensitizer is injected to patient's blood vessel, it can reach in abnormal new vessels with blood flow, then use a kind of special non-thermal energy laser (cold laser) to irradiate, thereby destroy abnormal new vessels.Optical dynamic therapy has controlled height, and device is simple, and cost is low, and the advantage that toxic and side effect is relatively little, once the great attention that proposes to be just subject to whole world scholar.Many new progresses have been obtained in the external smooth power of recent year field.Optical dynamic therapy has been applied to the treatment of tumour, and also has a large amount of research well afoots, has more wide application prospect in future.But the mechanism of optical dynamic therapy is still not clear at present, parameter waits to optimize, and also has a large amount of experiments to carry out before being applied to clinical treatment.This just needs a set of complete, adapt to various experiments, accurate experimental provision.
Fluorometry based on fluorescent marker is the important means in medical research, and the fluorescence that can reflect this substance characteristics being occurred after utilizing Cucumber by UV-irradiation, can carry out qualitative or quantitative test.At present, for the research of laser and optical dynamic therapy, adopt optical dynamic therapy to intervene the method that laggard line correlation fluorescent marker detects more.But this can not reflect the dynamic change of the label fluorescence in intervention completely.
Therefore, how to provide a kind of device, can Real-Time Monitoring intervention in fluorescence dynamic change, just become those skilled in the art's problem demanding prompt solution.
Utility model content
The utility model provides the fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment, in order to fluorescence dynamic change in the intervention of Real-Time Monitoring laser and optical dynamic therapy experiment.
For achieving the above object, the utility model provides the fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment, comprises generating device of laser, fixed mount, fluorospectrophotometer, lucifuge plate, laser emission probe, cuvette, wherein:
Described fluorospectrophotometer comprises Ear Mucosa Treated by He Ne Laser Irradiation module and signal acquisition module; Described Ear Mucosa Treated by He Ne Laser Irradiation module upper surface has circular open, and its inner middle part is provided with room, for accommodating described cuvette; Described signal acquisition module comprises detecting device and signal gathering unit, described detecting device is located at described room place, the spectral signal sending for detection of the fluorescent marker in described cuvette, and the fluorescence spectrum signal detecting is passed to described signal gathering unit;
Described cuvette is located in described room, is equipped with photosensitizer solution and fluorescence probe in described cuvette, and in the time accepting Ear Mucosa Treated by He Ne Laser Irradiation, described photosensitizer solution and fluorescence probe generate active oxygen fluorescent marker, for detection;
The laser probe of described generating device of laser enters described fluorospectrophotometer by described circular open; Described fixed mount is for being fixed on described laser probe the position that is vertically positioned at described cuvette top; Described generating device of laser is converted into laser probe described in optical signals by electric signal and penetrates;
Described Ear Mucosa Treated by He Ne Laser Irradiation module top is provided with described lucifuge plate, described lucifuge plate is two, wherein in the middle of one side of every, there is semicircle groove, the described semicircle groove of two blocks of described lucifuge plates is combined corresponding to described circular open and is covered at described Ear Mucosa Treated by He Ne Laser Irradiation module upper surface, for described circular open being sealed to ensure completely the dark surrounds of described Ear Mucosa Treated by He Ne Laser Irradiation inside modules, and fixing described laser probe;
Described signal gathering unit is connected with data processing equipment signal, and the fluorescence signal detecting is passed to described data processing equipment by described signal gathering unit.
Wherein, described lucifuge plate is black shadow shield.
Compared with prior art, beneficial effect of the present invention is:
Fluorescence monitoring device in the experiment of laser of the present utility model and optical dynamic therapy, the monitoring that makes to study label fluorescence dynamic change in optical dynamic therapy process more accurately and reliable, operates easylier, and reduces the impact that in experimentation, non-empirical factor causes; This device is for lasing condition optimization in optical dynamic therapy experiment and formulate unified parameter and standard important in inhibiting, and optical dynamic therapy method is applied to the clinical huge impetus that has.
Brief description of the drawings
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the fluorescence monitoring apparatus structure schematic diagram in the laser of the utility model one embodiment and optical dynamic therapy experiment;
Fig. 2 is that the fluorescence monitoring device in the laser of the utility model one embodiment and optical dynamic therapy experiment is connected the fluorescence curve over time real-time monitoring with data processing equipment;
Fig. 3 is that the fluorescence monitoring device in the laser of another embodiment of the utility model and optical dynamic therapy experiment is connected the fluorescence curve over time real-time monitoring with data processing equipment.
Description of reference numerals: 1-generating device of laser; 2-fixed mount; 3-Ear Mucosa Treated by He Ne Laser Irradiation module; 4-shadow shield; 5-laser probe; 6-cuvette; 7-data processing equipment; 8-signal acquisition module.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not paying the every other embodiment obtaining under creative work prerequisite, all belong to the scope of the utility model protection.
Refer to Fig. 1, Fig. 1 is the fluorescence monitoring apparatus structure schematic diagram in the laser of an embodiment of the utility model and optical dynamic therapy experiment.
As shown in Figure 1, the utility model provides the fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment, comprises generating device of laser 1, fixed mount 2, fluorospectrophotometer, lucifuge plate 4, laser emission probe 5, cuvette 6, wherein:
Described fluorospectrophotometer comprises Ear Mucosa Treated by He Ne Laser Irradiation module 3 and signal acquisition module 8; Ear Mucosa Treated by He Ne Laser Irradiation module 3 upper surfaces have circular open, and its inner middle part is provided with room, for accommodating cuvette 6; Signal acquisition module 8 comprises detecting device and signal gathering unit, and described detecting device is located at described room place, the spectral signal sending for detection of the fluorescent marker in cuvette 6, and the fluorescence spectrum signal detecting is passed to described signal gathering unit;
Cuvette 6 is located in described room, is equipped with photosensitizer solution and fluorescence probe in cuvette 6, and in the time accepting Ear Mucosa Treated by He Ne Laser Irradiation, described photosensitizer solution and fluorescence probe generate active oxygen fluorescent marker, for detection;
The laser probe 5 of generating device of laser 1 enters described fluorospectrophotometer by described circular open; Fixed mount 2 is for being fixed on laser probe 5 position that is vertically positioned at cuvette 6 tops; Generating device of laser 1 is converted into optical signals laser probe 5 by electric signal and penetrates;
Ear Mucosa Treated by He Ne Laser Irradiation module 3 tops are provided with lucifuge plate 4, lucifuge plate 4 is two, wherein in the middle of one side of every, there is semicircle groove, the described semicircle groove of two blocks of lucifuge plates 4 is combined corresponding to described circular open and is covered at Ear Mucosa Treated by He Ne Laser Irradiation module 3 upper surfaces, for described circular open being sealed to ensure completely the dark surrounds of Ear Mucosa Treated by He Ne Laser Irradiation module 3 inside, and fixed laser probe 5;
Described signal gathering unit is connected with data processing equipment 7 signals, and the fluorescence signal detecting is passed to data processing equipment 7 by described signal gathering unit.
In one embodiment of the invention, lucifuge plate 4 is black shadow shield.
Fig. 2 is that the fluorescence monitoring device in the laser of the utility model one embodiment and optical dynamic therapy experiment is connected the fluorescence curve over time real-time monitoring with data processing equipment.In the present embodiment, carry out optical dynamic therapy taking UCNPs-Ce6 as photosensitizer, under different capacity, test, by being housed, the solution of photosensitizer UCNPs-Ce6 and the cuvette of fluorescence probe 6 be placed in the room of described fluorospectrophotometer, laser probe 5 is put into described fluorospectrophotometer by described circular open, and laser probe 5 is fixed on to the position that is vertically positioned at cuvette 6 tops by fixed mount 2; The described semicircle groove of two blocks of lucifuge plates 4 is combined corresponding to described circular open and covered at Ear Mucosa Treated by He Ne Laser Irradiation module 3 upper surfaces, described circular open is sealed to ensure completely to the dark surrounds of Ear Mucosa Treated by He Ne Laser Irradiation module 3 inside, simultaneously fixed laser probe 5.Open the switch of generating device of laser 1, generating device of laser 1 is converted into optical signals laser probe 5 by electric signal and penetrates; The described detecting device of being located at described room place detects the spectral signal that the fluorescent marker of the UCNPs-Ce6 solution in cuvette 6 sends, and the fluorescence spectrum signal detecting is passed to described signal gathering unit, described signal gathering unit is connected with data processing equipment 7 signals, and the fluorescence signal detecting is passed to data processing equipment 7 by described signal gathering unit.In the present embodiment, data processing equipment 7 is computing machines.The fluorescence curve over time of active oxygen fluorescent marker DCFH-DA in the solution detecting as seen from Figure 2.The wherein not temporal evolution of fluorescence intensity of simple medicine group (drug), simple laser group (laser) slightly raises in time, but optical dynamic therapy group (different capacity 1W, 2W, 3W) fluorescence intensity is all higher than other groups.Repeat experiment and repeatedly all obtain identical result, confirmed the stability of device.
Fig. 3 is that the fluorescence monitoring device in the laser of another embodiment of the utility model and optical dynamic therapy experiment is connected the fluorescence curve over time real-time monitoring with data processing equipment.In the present embodiment, carry out optical dynamic therapy taking UCNPs-Ce6 as photosensitizer, under variable concentrations, test, by being housed, the solution of photosensitizer UCNPs-Ce6 and the cuvette of fluorescence probe 6 be placed in the room of described fluorospectrophotometer, laser probe 5 is put into described fluorospectrophotometer by described circular open, and laser probe 5 is fixed on to the position that is vertically positioned at cuvette 6 tops by fixed mount 2; The described semicircle groove of two blocks of lucifuge plates 4 is combined corresponding to described circular open and covered at Ear Mucosa Treated by He Ne Laser Irradiation module 3 upper surfaces, described circular open is sealed to ensure completely to the dark surrounds of Ear Mucosa Treated by He Ne Laser Irradiation module 3 inside, simultaneously fixed laser probe 5.Open the switch of generating device of laser 1, generating device of laser 1 is converted into optical signals laser probe 5 by electric signal and penetrates; The described detecting device of being located at described room place detects the spectral signal that the fluorescent marker of the UCNPs-Ce6 solution in cuvette 6 sends, and the fluorescence spectrum signal detecting is passed to described signal gathering unit, described signal gathering unit is connected with data processing equipment 7 signals, and the fluorescence signal detecting is passed to data processing equipment 7 by described signal gathering unit.In the present embodiment, data processing equipment 7 is computing machines.The fluorescence curve over time of active oxygen fluorescent marker DCFH-DA in the solution detecting as seen from Figure 3.The wherein not temporal evolution of fluorescence intensity of simple medicine group (drug), simple laser group (laser) slightly raises in time, but optical dynamic therapy group (variable concentrations 16 μ g/mL, 32 μ g/mL, 64 μ g/mL) fluorescence intensity is all higher than other groups.Repeat experiment and repeatedly all obtain identical result, confirmed the stability of device.
One of ordinary skill in the art will appreciate that: accompanying drawing is the schematic diagram of an embodiment, the module in accompanying drawing or flow process might not be that enforcement the utility model is necessary.
One of ordinary skill in the art will appreciate that: the module in the device in embodiment can be described and be distributed in the device of embodiment according to embodiment, also can carry out respective change and be arranged in the one or more devices that are different from the present embodiment.The module of above-described embodiment can be merged into a module, also can further split into multiple submodules.
Finally it should be noted that: above embodiment only, in order to the technical solution of the utility model to be described, is not intended to limit; Although the utility model is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these amendments or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of the utility model embodiment technical scheme.
Claims (2)
1. the fluorescence monitoring device in laser and optical dynamic therapy experiment, is characterized in that, comprises generating device of laser, fixed mount, fluorospectrophotometer, lucifuge plate, laser emission probe, cuvette, wherein:
Described fluorospectrophotometer comprises Ear Mucosa Treated by He Ne Laser Irradiation module and signal acquisition module; Described Ear Mucosa Treated by He Ne Laser Irradiation module upper surface has circular open, and its inner middle part is provided with room, for accommodating described cuvette; Described signal acquisition module comprises detecting device and signal gathering unit, described detecting device is located at described room place, the spectral signal sending for detection of the fluorescent marker in described cuvette, and the fluorescence spectrum signal detecting is passed to described signal gathering unit;
Described cuvette is located in described room, is equipped with photosensitizer solution and fluorescence probe in described cuvette, and in the time accepting Ear Mucosa Treated by He Ne Laser Irradiation, described photosensitizer solution and fluorescence probe generate active oxygen fluorescent marker, for detection;
The laser probe of described generating device of laser enters described fluorospectrophotometer by described circular open; Described fixed mount is for being fixed on described laser probe the position that is vertically positioned at described cuvette top; Described generating device of laser is converted into laser probe described in optical signals by electric signal and penetrates;
Described Ear Mucosa Treated by He Ne Laser Irradiation module top is provided with described lucifuge plate, described lucifuge plate is two, wherein in the middle of one side of every, there is semicircle groove, the described semicircle groove of two blocks of described lucifuge plates is combined corresponding to described circular open and is covered at described Ear Mucosa Treated by He Ne Laser Irradiation module upper surface, for described circular open being sealed to ensure completely the dark surrounds of described Ear Mucosa Treated by He Ne Laser Irradiation inside modules, and fixing described laser probe;
Described signal gathering unit is connected with data processing equipment signal, and the fluorescence signal detecting is passed to described data processing equipment by described signal gathering unit.
2. the fluorescence monitoring device in laser according to claim 1 and optical dynamic therapy experiment, is characterized in that, described lucifuge plate is black shadow shield.
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| CN201420415758.7U CN204008469U (en) | 2014-07-25 | 2014-07-25 | Fluorescence monitoring device in a kind of laser and optical dynamic therapy experiment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568893A (en) * | 2015-01-24 | 2015-04-29 | 北京中拓机械集团有限责任公司 | High-speed fluorescence spectrum detection device for semiconductor wafer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568893A (en) * | 2015-01-24 | 2015-04-29 | 北京中拓机械集团有限责任公司 | High-speed fluorescence spectrum detection device for semiconductor wafer |
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