CN203960197U - Tightness system and gene-amplificative instrament for gene-amplificative instrament - Google Patents
Tightness system and gene-amplificative instrament for gene-amplificative instrament Download PDFInfo
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- CN203960197U CN203960197U CN201420385886.1U CN201420385886U CN203960197U CN 203960197 U CN203960197 U CN 203960197U CN 201420385886 U CN201420385886 U CN 201420385886U CN 203960197 U CN203960197 U CN 203960197U
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- gene
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- amplificative instrament
- test tube
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Abstract
The utility model provides a kind of tightness system for gene-amplificative instrament and gene-amplificative instrament, and tightness system comprises that heat lid, heat cover and offers several and can insert invisible spectro projection.When carrying out polymerase chain reaction, the test tube that reaction reagent is housed is put in alternating temperature module, cover heat lid, in each test tube, all insert a projection matching with it, make invisible spectro reaction reagent and amplification system and external environment isolation, reduce the temperature impact of external environment on reaction reagent and amplification system, obtained good sealing effectiveness, avoided the minimizing of amplification system.
Description
Technical field
The utility model relates to polymerase chain reaction field, particularly relates to a kind of tightness system for gene-amplificative instrament and gene-amplificative instrament.
Background technology
In molecular biosciences field, gene-amplificative instrament polymerase chain reaction (PCR, Polymerase Chain Reaction) gains universal acceptance, is the effective means with high reliability of a kind of DNA of exploration.DNA in sample, under reagent coordinates, carries out temperature cycle reaction under specified temp program, can obtain at short notice the fragment amplification of 2 Nth power quantity.
The gene-amplificative instrament of main flow is substantially all from top to bottom in the market, sealer test tube is put into alternating temperature module, adopt semiconductor cooler at the bottom-heated of alternating temperature module, above sealer test tube, be stamped the heat lid for preventing the evaporation of sealer reaction in test tube system or overflowing, heat lid is tabular, and directly covers on sealer test tube.But the height of a plurality of sealer test tubes is inconsistent, thereby cause part sealer test tube by heat lid, not covered, make alternating temperature module stopping property when heating up inadequate, reaction system is easily evaporated or is overflowed.
Utility model content
Based on this, be necessary for the problems referred to above, a kind of tightness system for gene-amplificative instrament and gene-amplificative instrament are provided, should when testing, can prevent reaction system evaporation or overflow for tightness system and the gene-amplificative instrament of gene-amplificative instrament.
For a tightness system for gene-amplificative instrament, comprise the heat lid that is placed in alternating temperature module top, described hot capping offers some projections that in vitro seal test tube of inserting on the face of alternating temperature module.
In an embodiment, the described tightness system for gene-amplificative instrament also comprises the temperature control hot-plate to the heating of described heat lid therein, and described temperature control hot-plate is placed in the described heat side of covering.。
In an embodiment, the described tightness system for gene-amplificative instrament also comprises press box therein, and described press box is fixed on support.
In an embodiment, the described tightness system for gene-amplificative instrament also comprises stage clip therein, and described stage clip one end is placed in the described heat side of covering, and the other end is placed on described support.
In an embodiment, described heat lid is rectangle tabular therein.
In an embodiment, the cross-sectional shape of described projection is circle therein.
In an embodiment, described heat lid is made by the non-metallic material with thermal conductivity therein.
A kind of gene-amplificative instrament, comprise support and can insert the alternating temperature module of some test tubes, described gene-amplificative instrament also comprises tightness system, described tightness system comprises heat lid, described heat lid is placed in described alternating temperature module top, and described hot capping can insert to offering several on the face of described alternating temperature module the projection that in vitro seals test tube
In an embodiment, described tightness system also comprises press box therein, and described press box is fixed on described support, and described alternating temperature module is placed in described press box.
In an embodiment, described tightness system also comprises stage clip therein, and described stage clip one end is placed in the described heat side of covering, and the other end is placed on described support.
The above-mentioned tightness system for gene-amplificative instrament comprises that heat lid, heat cover and offers several and can insert invisible spectro projection.When carrying out polymerase chain reaction, the test tube that reaction reagent is housed is put in alternating temperature module, cover heat lid, in each test tube, all insert a projection matching with it, make invisible spectro reaction reagent and amplification system and external environment isolation, reduce the temperature impact of external environment on reaction reagent and amplification system, obtained good sealing effectiveness, the generation of having avoided amplification system to reduce.
Said gene amplification instrument comprises tightness system, and tightness system comprises that heat lid, heat cover and offers several and can insert invisible spectro projection.When carrying out polymerase chain reaction, the test tube that reaction reagent is housed is put in alternating temperature module, cover heat lid, in each test tube, all insert a projection matching with it, make invisible spectro reaction reagent and amplification system and external environment isolation, reduce the temperature impact of external environment on reaction reagent and amplification system, obtained good sealing effectiveness, avoided the minimizing of amplification system.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of gene-amplificative instrament in the utility model;
Fig. 2 is heat lid schematic diagram of the present utility model.
Embodiment
For above-mentioned purpose of the present utility model, feature and advantage can be become apparent more, below in conjunction with accompanying drawing, embodiment of the present utility model is described in detail.A lot of details have been set forth in the following description so that fully understand the utility model.But the utility model can be implemented to be much different from alternate manner described here, those skilled in the art can do similar improvement without prejudice to the utility model intension in the situation that, so the utility model is not subject to the restriction of following public specific embodiment.
It should be noted that, when element is called as " being fixed on " another element, can directly can there is element placed in the middle in it on another element or also.When an element is considered to " connection " another element, it can be directly connected to another element or may have centering elements simultaneously.
Unless otherwise defined, all technology of using are herein identical with the implication that belongs to the common understanding of those skilled in the art of the present utility model with scientific terminology.The term using in specification sheets of the present utility model herein, just in order to describe the object of specific embodiment, is not intended to be restriction the utility model.Term as used herein " and/or " comprise one or more relevant Listed Items arbitrarily with all combinations.
Please refer to Fig. 1, gene-amplificative instrament 100 comprises support 110, can insert the alternating temperature module 120 of some test tubes 200, well heater 130 and scatterer, well heater 130 is connected with alternating temperature module 120, well heater 130 adds thermogenetic thermal energy transfer to alternating temperature module 120, alternating temperature module 120 by thermal energy transfer to test tube 200, last test tube 200 is placed in the reaction reagent in test tube 200 by thermal energy transfer, and now reaction reagent is heated, and starts to carry out polymerase chain reaction.Yet polymerase chain reaction is the technology of a high low temperature, so these gene-amplificative instrament 100 use scatterers come to the reaction reagent cooling in test tube 200, to meet the condition of polymerase chain reaction.
The utility model provides a kind of tightness system for gene-amplificative instrament.When carrying out polymerase chain reaction, tightness system with this for gene-amplificative instrament seals test tube, can so that each test tube when carrying out polymerase chain reaction all airtight, be hedged off from the outer world, thereby the situation that can avoid the evaporation of reacted amplification system or overflow.
Please refer to Fig. 1 and Fig. 2, tightness system comprises the heat lid 151 that is placed in alternating temperature module 120 tops, and heat lid 151 is rectangle tabular.Heat lid 151 surfaces towards alternating temperature module 120 are called bottom surface, offer the projection 1511 that several can insert the interior sealing test tube 200 of test tube 200 on this bottom surface.The number of projection 1511 and the distributing position on heat lid 151 are all to determine according to number and the distributing position of insertable test tube 200 in alternating temperature module 120, as long as can guarantee that each test tube 200 has the projection 1511 that can insert wherein.While carrying out polymerase chain reaction, after reaction reagent is joined in test tube 200, test tube 200 is put in alternating temperature module 120, then covers heat lid 151, make to be all inserted with protruding 1511 in each test tube 200.Projection 1511 size shape is all corresponding with caliber size and the shape of test tube 200, so just can guarantee to be inserted into test tube 200 when interior when protruding 1511, can seal test tube 200, and the reaction reagent of test tube 200 and external environment are isolated.Conventionally 200 mouthfuls, test tube is all circular, thus the cross-sectional shape of the projection 1511 in present embodiment corresponding with test tube 200 be also circle.Certainly, in other embodiments, can also be other shapes, as long as can seal test tube 200.In addition, heat lid 151 can also be the shape such as circular, square, does not do concrete restriction herein, as long as the shape of heat lid 151 can make the satisfied conditions of offering the projection corresponding with test tube 200 1511 of heat lid 151.
Tightness system also comprises temperature control hot-plate 152 that can self-heating, and this temperature control hot-plate 152 is connected with heat lid 151.Temperature control hot-plate 152, for heat lid 151, is exactly well heater 130.Temperature control hot-plate 152 self-heatings, give the thermal energy transfer of its generation on the heat lid 151 being attached thereto, and this just requires heat lid 151 to have heat conductivility.Heat lid 151 is to be made by the non-metallic material with thermal conductivity, such as aluminum alloy materials or pottery etc.In other embodiments, heat lid 151 itself just can self-heating, so just can save temperature control hot-plate 152, and the while also can complete its corresponding function, and concrete manifestation is not specifically limited herein.
Tightness system also comprises press box 153, and press box 153 is fixed on support 110, and alternating temperature module 120 is placed in this press box 153, and the now effect of press box 153 is carrying alternating temperature modules 120.Tightness system also comprises stage clip 154, and one end of stage clip 154 is placed on support 110, and the other end is placed on temperature control hot-plate 152, and in other embodiments, when temperature control hot-plate 152 is integrated with heat lid 151, the other end of stage clip 154 is placed on heat lid 151.When in test tube 200, because temperature raises, pressure increases, heat lid 151 may be backed down and destroyed the stopping property in test tube 200.Now, the temperature control hot-plate 152 that covers 151 tops due to heat is being pressed by stage clip 154, and when pressure in test tube 200 increases heat lid 151 and upwards pushed up, stage clip 154 can continue heat lid 151 to press down, and guarantees the stopping property in test tube 200.If but the power of stage clip 154 during much larger than pressure in test tube 200, alternating temperature module 120 and on test tube 200 may all can be pressed down and mobile dislocation.Because alternating temperature module 120 is placed in press box 153, and press box 153 is fixed on support 110, thus alternating temperature module 120 and on test tube 200 can not be moved dislocation.In present embodiment, the quantity of stage clip 154 is 2, and in other embodiments, the quantity of stage clip 154 does not limit, as long as can reach its corresponding function.
The above-mentioned tightness system for gene-amplificative instrament, by the projection 1511 of offering on heat lid 151 is inserted in test tube 200, realizes the sealing to test tube 200.When carrying out polymerase chain reaction, the test tube 200 that reaction reagent is housed is put in alternating temperature module 120, cover heat lid 151, in each test tube 200, all insert a projection matching with it 1511, make reaction reagent and amplification system and external environment isolation in test tube 200, reduce the temperature impact of external environment on reaction reagent and amplification system, obtained good sealing effectiveness, avoided the situation that occurs that amplification system reduces.
Please refer to Fig. 1, the utility model provides a kind of gene-amplificative instrament 100.This gene-amplificative instrament 100 comprises the above-mentioned tightness system for gene-amplificative instrament, and on itself and gene-amplificative instrament 100, other interelement annexations, interactively etc. have all been described at the above-mentioned tightness system for gene-amplificative instrament, repeat no more herein.
Said gene amplification instrument 100 comprises tightness system, and tightness system comprises heat lid 151, on heat lid 151, offers the projection 1511 that several can insert the interior sealing test tube 200 of test tube 200.When carrying out polymerase chain reaction, the test tube 200 that reaction reagent is housed is put in alternating temperature module 120, cover heat lid 151, in each test tube 200, all insert a projection matching with it 1511, make reaction reagent and amplification system and external environment isolation in test tube 200, reduce the temperature impact of external environment on reaction reagent and amplification system, obtained good sealing effectiveness, avoided the minimizing of amplification system.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (10)
1. for a tightness system for gene-amplificative instrament, it is characterized in that, comprise the heat lid that is placed in alternating temperature module top, described hot capping offers some projections that in vitro seal test tube of inserting on the face of alternating temperature module.
2. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, the described tightness system for gene-amplificative instrament also comprises the temperature control hot-plate to the heating of described heat lid, and described temperature control hot-plate is placed in the described heat side of covering.
3. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, the described tightness system for gene-amplificative instrament also comprises press box, and described press box is fixed on support.
4. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, the described tightness system for gene-amplificative instrament also comprises stage clip, and described stage clip one end is placed in the described heat side of covering, and the other end is placed on described support.
5. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, described heat lid is rectangle tabular.
6. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, the cross-sectional shape of described projection is circle.
7. the tightness system for gene-amplificative instrament according to claim 1, is characterized in that, described heat lid is made by the non-metallic material with thermal conductivity.
8. a gene-amplificative instrament, comprise support and can insert the alternating temperature module of some test tubes, it is characterized in that, described gene-amplificative instrament also comprises tightness system, described tightness system comprises heat lid, described heat lid is placed in described alternating temperature module top, and described hot capping can insert to offering several on the face of described alternating temperature module the projection that in vitro seals test tube.
9. gene-amplificative instrament according to claim 8, is characterized in that, described tightness system also comprises press box, and described press box is fixed on described support, and described alternating temperature module is placed in described press box.
10. gene-amplificative instrament according to claim 8, is characterized in that, described tightness system also comprises stage clip, and described stage clip one end is placed in the described heat side of covering, and the other end is placed on described support.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420385886.1U CN203960197U (en) | 2014-07-11 | 2014-07-11 | Tightness system and gene-amplificative instrament for gene-amplificative instrament |
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| CN201420385886.1U CN203960197U (en) | 2014-07-11 | 2014-07-11 | Tightness system and gene-amplificative instrament for gene-amplificative instrament |
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| CN203960197U true CN203960197U (en) | 2014-11-26 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106318865A (en) * | 2016-10-25 | 2017-01-11 | 西安交通大学 | Portable device for nucleic acid extraction and gene amplification |
| CN107058090A (en) * | 2017-04-27 | 2017-08-18 | 滨江华康（北京）生物科技有限公司 | A kind of real-time fluorescence quantitative PCR gene magnification detector |
| CN112642501A (en) * | 2020-12-18 | 2021-04-13 | 南京溯远基因科技有限公司 | Thermal three-dimensional temperature equalizing cabin module for biochemical reaction |
-
2014
- 2014-07-11 CN CN201420385886.1U patent/CN203960197U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106318865A (en) * | 2016-10-25 | 2017-01-11 | 西安交通大学 | Portable device for nucleic acid extraction and gene amplification |
| CN106318865B (en) * | 2016-10-25 | 2018-03-16 | 西安交通大学 | A kind of nucleic acid extraction and the portable unit of gene magnification |
| CN107058090A (en) * | 2017-04-27 | 2017-08-18 | 滨江华康（北京）生物科技有限公司 | A kind of real-time fluorescence quantitative PCR gene magnification detector |
| CN112642501A (en) * | 2020-12-18 | 2021-04-13 | 南京溯远基因科技有限公司 | Thermal three-dimensional temperature equalizing cabin module for biochemical reaction |
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