CN203858248U - Procalcitonin test strip - Google Patents
Procalcitonin test strip Download PDFInfo
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- CN203858248U CN203858248U CN201420290850.5U CN201420290850U CN203858248U CN 203858248 U CN203858248 U CN 203858248U CN 201420290850 U CN201420290850 U CN 201420290850U CN 203858248 U CN203858248 U CN 203858248U
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Abstract
The utility model discloses a procalcitonin test strip which comprises a PVC bottom plate, a sample pad, a fluorescent pad, a piece of water absorption filter paper, a nitrocellulose membrane, a quality control line and a detection line, wherein the PVC bottom plate is arranged at the bottom end of the whole test strip; the sample pad is provided with a sampling area; the right end of the sample pad is in lap joint with the left end of the fluorescent pad; the fluorescent pad is enveloped by a protein antibody marked by fluorescent light; the right end of the fluorescent pad is in lap joint with the left end of the nitrocellulose membrane; the water absorption filter paper is in lap joint with the right end of the nitrocellulose membrane; the quality control line and the detection line are respectively enveloped by a protein antibody and are respectively arranged on the nitrocellulose membrane. The procalcitonin test strip is used for measuring the content of procalcitonin by the principle of a double-antibody sandwich method, is high in sensitivity and is capable of repeatedly and quantitatively testing the content of the procalcitonin in the serum, plasma or whole blood of a human body; the procalcitonin test strip is simple, rapid and accurate in result; the procalcitonin test strip is simple in whole structure, convenient to operate, low in manufacturing cost and high in practicality.
Description
Technical field
The utility model relates to medical immunology in-vitro diagnosis applied technical field, especially a kind of Procalcitonin test strip.
Background technology
Procalcitonin (PCT) is without peptide material before the calcitonin of hormonal activity, consists of the glycoprotein that molecular mass is 13KD 116 amino acid.PCT secretes (comprising thyroid gland, lung and pancreatic tissue cell) by neural inner cell and expresses, and through enzyme, cuts and is decomposed into calcitonin (CT), c-terminal peptides and amino terminal peptide; The half life period of PCT in human body is 25-30h, have good stability, in normal human serum, content is extremely low, causing in many situations of systemic inflammatory response syndrome (SIRS), such as bacterial infection, pancreatitis, burn, multiple injury, in blood, before CT, all montage products of peptide material raise extremely, and wherein PCT is topmost product, and CT is without significant change.The order of severity of blood PCT level and infection and damage is proportionate, and has become for the diagnosis of pyemia, severe sepsis, septic shock and the efficiency index of curative effect monitoring; PCT has reflected the active degree of systemic inflammatory response.The factor that affects PCT level comprises size and type, the kind of bacterium, the degree of inflammation and the immunoreactive situation of infected organ.In addition, PCT just can measure at the large-scale surgical postoperative 1~4d of small number of patients; The rising of PCT level appears at serious shock, systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction derangement syndrome (MODS), even without bacterium, infects or bacillary focus.But PCT level is usually less than those patients who has bacillary focus in these cases; From the enteron aisle release cells factor or bacterial translocation, may cause induction; At present the detection method of Procalcitonin mainly be take biochemical instruments detects and collaurum detects method as main, and the speed of upper biochemical instruments is slow, and collaurum detection method is that semiquantitative method cannot meet current clinical bedside and diagnoses POCT demand; For making up the deficiencies in the prior art, the utility model aims to provide a kind of Procalcitonin test strip.
Utility model content
For the problems referred to above, the utility model aims to provide a kind of Procalcitonin test strip.
For realizing this technical purpose, scheme of the present utility model is: a kind of Procalcitonin test strip, comprises PVC base plate, sample pad, fluorescence pad, absorbent filter, nitrocellulose filter, nature controlling line and detection line; Described PVC base plate is located at the whole bottom of test strips; Described sample pad is provided with sample application zone, sample pad right-hand member and fluorescence pad left end overlap joint; Described fluorescence pad is coated with fluorescently-labeled protein antibodies, and its right-hand member and a nitrocellulose filter left side connect and connect; Described absorbent filter and nitrocellulose filter right-hand member overlap joint; Described nature controlling line and detection line are all coated with protein antibodies, and are arranged at respectively on nitrocellulose filter; Described sample pad, fluorescence pad, nitrocellulose filter and absorbent filter are connected with the PVC plate with gum respectively.
As preferably, on described fluorescence pad, coated fluorescence labeling protein antibodies is anti-human Procalcitonin monoclonal antibody; On nature controlling line and detection line, coated protein antibodies is respectively protein antibodies and is respectively rabbit anti-mouse igg antibody and anti-human Procalcitonin monoclonal antibody.
As preferably, described nature controlling line and detection line are arranged on nitrocellulose filter and are parallel to each other, and nature controlling line is away from fluorescence pad end, and detection line is near fluorescence pad end.
Compared with prior art, the beneficial effects of the utility model are: this Procalcitonin test strip is measured Procalcitonin content by double antibody sandwich method principle, its detection sensitivity is high, can repeat, quantitatively detect the content of Procalcitonin in human serum, blood plasma or whole blood; Easy fast and result accurate; One-piece construction is simple, easy to operate, and low cost of manufacture is practical.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model;
Wherein: 1, PVC base plate, 2, sample pad, 3, fluorescence pad, 4, absorbent filter, 5, nitrocellulose filter, 6, nature controlling line, 7, detection line.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, rather than whole embodiment.Embodiment based in the utility model, those of ordinary skills are not making all other embodiment that obtain under creative work prerequisite, all belong to the scope of the utility model protection.
Refer to Fig. 1, in the utility model embodiment, a kind of Procalcitonin test strip, comprises PVC base plate 1, sample pad 2, fluorescence pad 3, absorbent filter 4, nitrocellulose filter 5, nature controlling line 6 and detection line 7; Described PVC base plate 1 is located at the whole bottom of test strips; Described sample pad 2 is provided with sample application zone, sample pad 2 right-hand members and fluorescence pad 3 left end overlap joints; Described fluorescence pad 3 is coated with fluorescently-labeled protein antibodies, and its right-hand member and nitrocellulose filter 5 left sides connect and connect; Described absorbent filter 4 and nitrocellulose filter 5 right-hand member overlap joints; Described nature controlling line 6 is all coated with protein antibodies with detection line 7, and is arranged at respectively on nitrocellulose filter 5; Described sample pad 2, fluorescence pad 3, nitrocellulose filter 5 and absorbent filter 4 are connected with the PVC plate 8 with gum respectively.
As preferably, on described fluorescence pad 3, coated fluorescence labeling protein antibodies is anti-human Procalcitonin monoclonal antibody; Protein antibodies coated on nature controlling line 6 and detection line 7 is respectively rabbit anti-mouse igg antibody and anti-human Procalcitonin monoclonal antibody.
As preferably, described nature controlling line 6 and detection line 7 are arranged on nitrocellulose filter 5 and are parallel to each other, and nature controlling line 6 is away from fluorescence pad 3 ends, and detection line 7 is near fluorescence pad 3 ends.
Further, described Procalcitonin test strip, its method for making is: first, the preparation of each composition of test strips, it comprises the preparation (Procalcitonin monoclonal antibody being diluted to 1mg/ml with 10mMPB solution) of detection line solution and the preparation (Avidin being diluted to 0.5mg/ml with 10mMPB solution) of nature controlling line solution.Next carries out the preparation of test strips, its step comprises: step 1, and blank kilocalorie is pasted, and sample pad one end is overlapped on fluorescence pad, the fluorescence pad other end is overlapped on nitrocellulose filter, absorbent filter is overlapped on the nitrocellulose filter other end, and four parts stick on PVC plate in turn; Step 2: spray film, in detection line, nature controlling line position, spray respectively detection line, nature controlling line solution (detection line, nature controlling line spray film liquid measure are 1 μ l/cm); Step 3: dry, the Procalcitonin that has sprayed reagent is stuck in greatly in constant temperature oven to 37 ℃ dries 24 hours in step 2; Step 4: slitting, cuts into the Procalcitonin kilocalorie of oven dry the paper slip of 4mm width; Finally obtain Procalcitonin test strips.
To those skilled in the art, obviously the utility model is not limited to the details of above-mentioned one exemplary embodiment, and in the situation that not deviating from spirit of the present utility model or essential characteristic, can realize the utility model with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims rather than above-mentioned explanation, is therefore intended to include in the utility model dropping on the implication that is equal to important document of claim and all changes in scope.Any Reference numeral in claim should be considered as limiting related claim.
The above; it is only preferred embodiment of the present utility model; not in order to limit the utility model, any trickle modification that every foundation technical spirit of the present utility model is done above embodiment, be equal to and replace and improve, within all should being included in the protection domain of technical solutions of the utility model.
Claims (3)
1. a Procalcitonin test strip, comprises PVC base plate (1), sample pad (2), fluorescence pad (3), absorbent filter (4), nitrocellulose filter (5), nature controlling line (6) and detection line (7); It is characterized in that: described PVC base plate (1) is located at the whole bottom of test strips; Described sample pad (2) is provided with sample application zone, sample pad (2) right-hand member and fluorescence pad (3) left end overlap joint; Described fluorescence pad (3) is coated with fluorescently-labeled protein antibodies, and its right-hand member and nitrocellulose filter (5) left side connects and connects; Described absorbent filter (4) and nitrocellulose filter (5) right-hand member overlap joint; Described nature controlling line (6) is all coated with protein antibodies with detection line (7), and is arranged at respectively on nitrocellulose filter (5); Described sample pad (2), fluorescence pad (3), nitrocellulose filter (5) and absorbent filter (4) are connected with the PVC plate (8) with gum respectively.
2. a kind of Procalcitonin test strip according to claim 1, is characterized in that: the upper coated fluorescence labeling protein antibodies of described fluorescence pad (3) is anti-human Procalcitonin monoclonal antibody; Nature controlling line (6) is respectively rabbit anti-mouse igg antibody and anti-human Procalcitonin monoclonal antibody with the upper coated protein antibodies of detection line (7).
3. a kind of Procalcitonin test strip according to claim 1, it is characterized in that: described nature controlling line (6) and detection line (7) are arranged at nitrocellulose filter (5) above and are parallel to each other, nature controlling line (6) is away from fluorescence pad (3) end, and detection line (7) is near fluorescence pad (3) end.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104714017A (en) * | 2015-02-06 | 2015-06-17 | 北京豪迈生物工程有限公司 | Method for quantitatively detecting procalcitonin |
CN105949312A (en) * | 2016-06-07 | 2016-09-21 | 江苏众红生物工程创药研究院有限公司 | Anti-human pct antibody and application thereof |
CN112083161A (en) * | 2020-10-14 | 2020-12-15 | 芜湖森爱驰生物科技有限公司 | Procalcitonin (PCT) fluorescence detection kit and preparation process thereof |
-
2014
- 2014-05-26 CN CN201420290850.5U patent/CN203858248U/en active Active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104714017A (en) * | 2015-02-06 | 2015-06-17 | 北京豪迈生物工程有限公司 | Method for quantitatively detecting procalcitonin |
CN105949312A (en) * | 2016-06-07 | 2016-09-21 | 江苏众红生物工程创药研究院有限公司 | Anti-human pct antibody and application thereof |
CN112083161A (en) * | 2020-10-14 | 2020-12-15 | 芜湖森爱驰生物科技有限公司 | Procalcitonin (PCT) fluorescence detection kit and preparation process thereof |
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