CN203858247U

CN203858247U - N-terminal brain natriuretic peptide precursor detection test strip

Info

Publication number: CN203858247U
Application number: CN201420290818.7U
Authority: CN
Inventors: 胡容; 王忠亮
Original assignee: Anhui Hui Bang Bioengineering Inc Co
Current assignee: Anhui Hui Bang Bioengineering Inc Co
Priority date: 2014-05-26
Filing date: 2014-05-26
Publication date: 2014-10-01
Anticipated expiration: 2024-05-26

Abstract

The utility model discloses an N-terminal brain natriuretic peptide precursor detection test strip which comprises a PVC (polyvinyl chloride) base plate, a sample pad, a fluorescence pad, a piece of water absorption filtering paper, a nitrocellulose film, a quality control wire and a detection wire, wherein the PVC base plate is arranged at the bottom end of the whole test strip; the sample pad is provided with a sample injection region; the right end of the sample pad is overlapped with the left end of the fluorescence pad; the fluorescence pad is covered with a protein antibody marked by fluorescence, and the right end of the fluorescence pad is connected with the left end of the nitrocellulose film; the water absorption filtering paper is overlapped with the right end of the nitrocellulose film; the quality control wire and the detection wire are covered with protein antibodies and are respectively arranged on the nitrocellulose film. The N-terminal brain natriuretic peptide precursor detection test strip can be used for measuring the N-terminal brain natriuretic peptide precursor content through the principle of a double-antibody sandwich method, is high in detection sensitivity and high in repetitiveness, can be used for quantitatively detecting the content of N-terminal brain natriuretic peptide precursors in plasma, serum and full blood of a human body, can guarantee simple and quick detection and accurate results, and is simple in whole structure, convenient to operate and low in manufacturing cost and high in practicability.

Description

A kind of N-akrencephalon pro-BNP test strip

Technical field

The utility model relates to medical immunology in-vitro diagnosis applied technical field, especially a kind of N-akrencephalon pro-BNP test strip.

Background technology

N-akrencephalon pro-BNP (N-terminal Pro-Brain Natriuretic Peptide) is the pyrolysis product of proBNP after enzyme is cut, proBNP is cracked into N-akrencephalon pro-BNP and BNP under proteinase effect, and two peptide species all discharge into blood circulation.With respect to BNP, N-akrencephalon pro-BNP has longer plasma half-life (60-120 minute), and only have 20 minutes the plasma half-life of BNP.Secretion and the existence of N-akrencephalon pro-BNP in blood has cumulative function, and physical presence is higher than the concentration of BNP, is more easily detected, and the susceptibility detecting improves, and N-akrencephalon pro-BNP more easily reflects the variation of early stage or slight cardiac function.N-akrencephalon pro-BNP has lower individual difference, is not subject to the impact of the individual physiological rhythm and pace of moving things.External shelf stability is good, can reach 3 days under room temperature, to sample transport, preservation etc. is extremely important.Be not subject to collection of specimens condition (after crouching, sit, moving) restriction, use the blood plasma of serum or different anti-freezing (heparin, EDTA) all not affect measurement result.In addition, N-akrencephalon pro-BNP and exogenous BNP do not exist intersect active, still can be by the effect of N-akrencephalon pro-BNP monitor therapy while using BNP treatment; The order of severity of N-akrencephalon pro-BNP and clinical heart failure is proportional, and heart failure is more serious, and N-akrencephalon pro-BNP is just higher; And N-akrencephalon pro-BNP can be distinguished slight heart failure and the normal person of heart function.In view of N-akrencephalon pro-BNP is better than BNP to diagnosis feature in heart failure, about 2000, assert that in the world N-akrencephalon pro-BNP measures that of heart failure is epoch-making has a specific mark.What at present, in in-vitro diagnosis market, more authoritative and conventional N-akrencephalon pro-BNP test was Roche diagnostic companies is full-automatic nT-proBNP detects.This detection principle is that sandwich reaction and electrochemiluminescence detect, be designed for extensive centralab, and in order to test, except the liquid reagent of accurate measurement, also need the instrument of relative complex carry out quantitative liquid and detect luminous signal, operate more loaded down with trivial details and complicated; For making up the deficiencies in the prior art, the utility model aims to provide a kind of N-akrencephalon pro-BNP test strip.

Utility model content

For the problems referred to above, the utility model aims to provide a kind of N-akrencephalon pro-BNP test strip.

For realizing this technical purpose, scheme of the present utility model is: a kind of N-akrencephalon pro-BNP test strip, comprises PVC base plate, sample pad, fluorescence pad, absorbent filter, nitrocellulose filter, nature controlling line and detection line; Described PVC base plate is located at test strips entirety bottom; Described sample pad is provided with sample application zone, sample pad right-hand member and fluorescence pad left end overlap joint; Described fluorescence pad is coated with fluorescently-labeled protein antibodies, and its right-hand member and a nitrocellulose filter left side connect and connect; Described absorbent filter and nitrocellulose filter right-hand member overlap joint; Described nature controlling line and detection line are all coated with protein antibodies, and are arranged at respectively on nitrocellulose filter; Described sample pad, fluorescence pad, nitrocellulose filter and absorbent filter are connected with the PVC plate with gum respectively.

Further, on described fluorescence pad, coated fluorescence labeling protein antibodies is anti-human N-akrencephalon pro-BNP monoclonal antibody; On nature controlling line and detection line, coated protein antibodies is respectively rabbit anti-mouse igg antibody and anti-human N-akrencephalon pro-BNP monoclonal antibody.

Further, described nature controlling line and detection line are arranged on nitrocellulose filter and are parallel to each other, and nature controlling line is away from fluorescence pad end, and detection line is near fluorescence pad end.

Compared with prior art, the beneficial effects of the utility model are: this N-akrencephalon pro-BNP test strip is measured N-akrencephalon pro-BNP content by double antibody sandwich method principle, detection sensitivity is high and have good repeatability, the quantitatively content of N-akrencephalon pro-BNP in human body blood plasma, serum and whole blood; Easy fast and result accurate; One-piece construction is simple, easy to operate, and low cost of manufacture is practical.

Brief description of the drawings

Fig. 1 is one-piece construction schematic diagram of the present utility model;

Wherein: 1, PVC base plate, 2, sample pad, 3, fluorescence pad, 4, absorbent filter, 5, nitrocellulose filter, 6, nature controlling line, 7, detection line.

Embodiment

Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not making all other embodiment that obtain under creative work prerequisite, all belong to the scope of the utility model protection.

Refer to Fig. 1, in the utility model embodiment, a kind of N-akrencephalon pro-BNP test strip, comprises PVC base plate 1, sample pad 2, fluorescence pad 3, absorbent filter 4, nitrocellulose filter 5, nature controlling line 6 and detection line 7; Described PVC base plate 1 is located at test strips entirety bottom; Described sample pad 2 is provided with sample application zone, sample pad 2 right-hand members and fluorescence pad 3 left end overlap joints; Described fluorescence pad 3 is coated with fluorescently-labeled protein antibodies, and its right-hand member and nitrocellulose filter 5 left sides connect and connect; Described absorbent filter 4 and nitrocellulose filter 5 right-hand member overlap joints; Described nature controlling line 6 is all coated with protein antibodies with detection line 7, and is arranged at respectively on nitrocellulose filter 5; Described sample pad 2, fluorescence pad 3, nitrocellulose filter 5 and absorbent filter 4 are connected with the PVC plate 8 with gum respectively.

Further, on described fluorescence pad 3, coated fluorescence labeling protein antibodies is anti-human N-akrencephalon pro-BNP monoclonal antibody; Protein antibodies coated on nature controlling line 6 and detection line 7 is respectively rabbit anti-mouse igg antibody and anti-human N-akrencephalon pro-BNP monoclonal antibody.

Further, described nature controlling line 6 and detection line 7 are arranged on nitrocellulose filter 5 and are parallel to each other, and nature controlling line 6 is away from fluorescence pad 3 ends, and detection line 7 is near fluorescence pad 3 ends.

Further, described N-akrencephalon pro-BNP test strip, its method for making is: first, the preparation of the each composition of test strips, it comprises the preparation (Procalcitonin monoclonal antibody being diluted to 1mg/ml with 10mMPB solution) of detection line solution and the preparation of nature controlling line solution (Avidin being diluted to 0.5mg/ml with 10mMPB solution); Next carries out the preparation of test strips, its step comprises: step 1, and blank kilocalorie is pasted, and sample pad one end is overlapped on fluorescence pad, the fluorescence pad other end is overlapped on nitrocellulose filter, absorbent filter is overlapped on the nitrocellulose filter other end, and four parts stick on PVC plate in turn; Step 2: spray film, spray respectively detection line, nature controlling line solution (detection line, nature controlling line spray film liquid measure are 1 μ l/cm) in detection line, nature controlling line position; Step 3: dry, the Procalcitonin that has sprayed reagent is stuck in greatly in constant temperature oven to 37 DEG C dries 24 hours in step 2; Step 4: slitting, cuts into the Procalcitonin kilocalorie of oven dry the paper slip of 4mm width; Again carry out test strips assembling, on PVC base plate, pasting sample pad, fluorescence pad, nitrocellulose filter and thieving paper obtains test paper plate overlap joint mutually in turn, cuts into the test strips of proper width; Finally obtain N-akrencephalon pro-BNP test strip.

To those skilled in the art, obviously the utility model is not limited to the details of above-mentioned example embodiment, and in the situation that not deviating from spirit of the present utility model or essential characteristic, can realize the utility model with other concrete form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims instead of above-mentioned explanation, is therefore intended to all changes that drop in the implication and the scope that are equal to important document of claim to include in the utility model.Any Reference numeral in claim should be considered as limiting related claim.

The above; it is only preferred embodiment of the present utility model; not in order to limit the utility model, any trickle amendment that every foundation technical spirit of the present utility model is done above embodiment, be equal to and replace and improve, within all should being included in the protection domain of technical solutions of the utility model.

Claims

1. a N-akrencephalon pro-BNP test strip, comprises PVC base plate (1), sample pad (2), fluorescence pad (3), absorbent filter (4), nitrocellulose filter (5), nature controlling line (6) and detection line (7); It is characterized in that: described PVC base plate (1) is located at test strips entirety bottom; Described sample pad (2) is provided with sample application zone, sample pad (2) right-hand member and fluorescence pad (3) left end overlap joint; Described fluorescence pad (3) is coated with fluorescently-labeled protein antibodies, and its right-hand member and nitrocellulose filter (5) left side connects and connects; Described absorbent filter (4) and nitrocellulose filter (5) right-hand member overlap joint; Described nature controlling line (6) is all coated with protein antibodies with detection line (7), and is arranged at respectively on nitrocellulose filter (5); Described sample pad (2), fluorescence pad (3), nitrocellulose filter (5) and absorbent filter (4) are connected with the PVC plate (8) with gum respectively.

2. a kind of N-akrencephalon pro-BNP test strip according to claim 1, is characterized in that: the upper coated fluorescence labeling protein antibodies of described fluorescence pad (3) is anti-human N-akrencephalon pro-BNP monoclonal antibody; Nature controlling line (6) is respectively rabbit anti-mouse igg antibody and anti-human N-akrencephalon pro-BNP monoclonal antibody with the upper coated protein antibodies of detection line (7).

3. a kind of N-akrencephalon pro-BNP test strip according to claim 1, it is characterized in that: described nature controlling line (6) and detection line (7) are arranged at nitrocellulose filter (5) above and are parallel to each other, nature controlling line (6) is away from fluorescence pad (3) end, and detection line (7) is near fluorescence pad (3) end.