CN203833945U - Escherichia coli and/or coliform group testing slip - Google Patents
Escherichia coli and/or coliform group testing slip Download PDFInfo
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- CN203833945U CN203833945U CN201420242964.2U CN201420242964U CN203833945U CN 203833945 U CN203833945 U CN 203833945U CN 201420242964 U CN201420242964 U CN 201420242964U CN 203833945 U CN203833945 U CN 203833945U
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- testing plate
- coliform
- egative film
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Abstract
This utility model provides an Escherichia coli and/or coliform group testing slip, wherein the testing slip comprises a base slip 1, a non-woven fabric layer 2 printed with checks and located on the base slip, a culture medium 3 located on the nonwoven fabric layer and a cover film 4 containing a glucosidase indicator. By virtue of the Escherichia coli and/or coliform group testing slip provided by the utility model, Escherichia coli and/or coliform groups in food can be automatically detected; the detection testing cycle is also accelerated and thus the food and environmental protection departments quickly and accurately acquire the number of Escherichia coli and/or coliform groups in food.
Description
Technical field
The utility model belongs to food inspection and environment measuring field, relates in particular to a kind of intestinal bacteria and/or coliform testing plate.
Background technology
The food contamination problem that microorganism in food causes has been subject to extensive concern as the important content of food safety, in food production, from process for processing to transportation and sale, links is microbial contamination likely all, therefore essential to microorganism detection.But nowadays traditional microorganism detection method complex operation not only, sense cycle is long, also very high to testing staff's requirement, is difficult to meet the modern goods demand for development of circulation fast, has even affected the development of whole food service industry.
Utility model CN200520120355 patent provides the testing plate of pathogenic micro-organism in a kind of food, and it is comprised of absorbent filter and the selective coloration culture medium layer that contains water absorbent gel.
But this area still needs testing plate of the prior art to improve, accurately quick and more excellent intestinal bacteria and/or the coliform testing plate of performance so that a kind of detection to be provided.
Utility model content
Therefore, the utility model provides a kind of intestinal bacteria and/or coliform testing plate, wherein, described testing plate comprises egative film 1, is positioned at the nonwoven layer that is printed on grid 2 on egative film, the epiphragma 4 that is positioned at the substratum 3 in nonwoven layer and contains glucuroide indicator.
Those skilled in the art it is generally acknowledged that coliform bacterium can comprise intestinal bacteria, citric acid bacillus, the white Salmonella of aerogenesis Cray and enterobacter cloacae etc.The intestinal bacteria that the utility model provides and/or coliform testing plate, can detect separately coliform count, also can in once testing, detect the sum that obtains coliform.
Substratum in the utility model can be substratum arbitrarily in prior art, as long as be applicable to the substratum that intestinal bacteria and/or coliform cultivate, all can be used in the utility model.For example, in a concrete embodiment, the substratum in the utility model is VRB substratum.
Glucuroide indicator in the utility model can be glucuroide indicator arbitrarily in prior art, at this, does not do any restriction.
In a concrete embodiment, described substratum 3 being distributed as in described nonwoven layer covers a border circular areas 5 at least completely.Preferably, described border circular areas 5 is positioned at the central authorities of described egative film.Preferably, the area of described border circular areas is 1/5～4/5 of described egative film area, for example, be 1/3～2/3.In a specific embodiment, the number of the complete grid in described border circular areas is 5～30, and preferably this number is 15～20.In a kind of embodiment, in described testing plate, also comprise that one covers the middle layer of described nonwoven layer with manhole and part.Described middle layer is between described nonwoven layer and described epiphragma, on it with manhole and size and the position of the border circular areas in the utility model 5 match, this middle layer is for hiding the part beyond the border circular areas in egative film and nonwoven layer 5.
In a kind of concrete embodiment, described egative film is that the scraps of paper or described egative film consist of water-tight and air-tight synthetic materials.In another embodiment, in described epiphragma, contain coldwater-soluble gel and glucuroide indicator.
In addition, in the utility model, between described egative film 1, nonwoven layer 2 and epiphragma 4, optionally use tackiness agent to connect.
Intestinal bacteria and/or the coliform testing plate of using the utility model to provide, not only can measure intestinal bacteria and/or coliform in food automatically; Also can accelerate to detect test period, make food and environmental administration know rapidly and accurately intestinal bacteria and/or the coliform group count in food.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of intestinal bacteria and/or the test piece of coliform Quick Measuring in the utility model.Wherein, 1, egative film; 2, be printed on the nonwoven layer of grid; 3, substratum, 4, epiphragma; 5, border circular areas.
Embodiment
As shown in Figure 1, the intestinal bacteria that provide in the utility model and/or the test piece of coliform Quick Measuring contain the egative film 1 consisting of water-tight and air-tight synthetic materials, the nonwoven layer 2 that is printed on grid, VRB substratum 3, the epiphragma 4 that contains coldwater-soluble gel and glucuroide indicator and border circular areas 5, and in this testing plate, also contain a middle layer with manhole.In this embodiment, the number of the complete grid in described border circular areas is 20 (not shown corresponding graticule lines).
When the intestinal bacteria in use the utility model and/or the test piece of coliform Quick Measuring, relate to inoculation, microorganism culture and count three steps.Particularly, first take or draw food samples to be measured, being placed in suitable sterile chamber, adding appropriate sterile diluent, stirred sample to be adjusted to pH value with diluent be 6.5-7.5; Testing sample is prepared into 1:10 or larger dilution sample diluting liquid.Above-mentioned testing plate is placed on desktop, open epiphragma, with suction pipe, draw 1ml samples vertical and drip the border circular areas place in testing plate, and under epiphragma is slowly covered, should avoid alveolate generation, with pressing plate, be pressed in epiphragma centre, depress gently, make to treat that sample measuring liquid uniform fold is on described border circular areas, standing 1-2min makes culture medium solidifying.Then testing plate being put into incubator cultivates.The method such as available counting by naked eye or standard colony counter counting after cultivation completes.The suitable counting region of this testing plate colony number is 15-150.
Those skilled in the art are known, and most colibacillus group energys produce beta-glucosidases, and it and described glucuroide indicator reaction produce blue precipitation ring around intestinal bacteria periphery of bacterial colonies.The epiphragma of surface coverage (glued membrane), can keep the gas that intestinal bacteria and/or coliform ferment lactose produce here, forms blueness with blue red bacterium colony and has bubble phase to be connected.Wherein, blue colonies is Escherichia coli flora with bubble, and total be with alveolate redness and blue colonies are coliform group count.That is to say, the intestinal bacteria that the utility model provides and/or coliform testing plate, can detect separately intestinal bacteria, now needs the blue colonies counting in grid in the testing plate after cultivating; Use the testing plate in the utility model, also can, with once detecting the sum that obtains coliform in experiment, now need the blue colonies in grid in the testing plate after cultivating and total counting number of red bacterium colony.
The testing plate that the utility model provides only needs inoculation, microorganism culture and count three steps can realize the mensuration fast and accurately to intestinal bacteria and/or coliform, improves sense cycle and the working efficiency of food and environmental administration's microorganism testing.
Testing plate in the utility model can be that polylith testing plate is packaging together, for example, be packaged in same packing box.Contain in the utility model when the packing box of testing plate does not break a seal, this packing box should refrigerate in being less than under the environment of 8 ℃.If this packing box has broken a seal and wherein also contain untapped testing plate, application of tape is tamping the sealing of this packing box, and in 1 month, is finished remaining testing plate.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (10)
1. intestinal bacteria and/or coliform testing plate, it is characterized in that, described testing plate comprises egative film (1), is positioned at the nonwoven layer that is printed on grid (2) on egative film, the epiphragma (4) that is positioned at the substratum (3) in nonwoven layer and contains glucuroide indicator.
2. testing plate according to claim 1, is characterized in that, described substratum (3) being distributed as in described nonwoven layer covers a border circular areas (5) at least completely.
3. testing plate according to claim 2, is characterized in that, the area of described border circular areas is 1/5～4/5 of described egative film area.
4. testing plate according to claim 2, is characterized in that, the number of the complete grid in described border circular areas is 5～30.
5. testing plate according to claim 4, is characterized in that, the number of the complete grid in described border circular areas is 15～20.
6. testing plate according to claim 2, is characterized in that, described testing plate also comprises a middle layer that covers described nonwoven layer with manhole and part.
7. according to the testing plate described in any one in claim 1～6, it is characterized in that, described egative film is that the scraps of paper or described egative film consist of water-tight and air-tight synthetic materials.
8. according to the testing plate described in any one in claim 1～6, it is characterized in that, described substratum is VRB substratum.
9. according to the testing plate described in any one in claim 1～6, it is characterized in that, in described epiphragma, contain coldwater-soluble gel and glucuroide indicator.
10. according to the testing plate described in any one in claim 1～6, it is characterized in that, between described egative film (1), nonwoven layer (2) and epiphragma (4), optionally use tackiness agent to connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420242964.2U CN203833945U (en) | 2014-05-13 | 2014-05-13 | Escherichia coli and/or coliform group testing slip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420242964.2U CN203833945U (en) | 2014-05-13 | 2014-05-13 | Escherichia coli and/or coliform group testing slip |
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| Publication Number | Publication Date |
|---|---|
| CN203833945U true CN203833945U (en) | 2014-09-17 |
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| CN201420242964.2U Expired - Fee Related CN203833945U (en) | 2014-05-13 | 2014-05-13 | Escherichia coli and/or coliform group testing slip |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754849A (en) * | 2016-03-31 | 2016-07-13 | 吉林农业大学 | Total bacterial count testing slip and preparation method and application thereof |
| CN106520542A (en) * | 2016-12-16 | 2017-03-22 | 天津科技大学 | Total bacterial count test piece and manufacturing method thereof |
| CN106802290A (en) * | 2016-11-29 | 2017-06-06 | 武汉市宇驰检测技术有限公司 | A kind of fluorescence spectrophotometry that E. CoIi content is detected based on carbon quantum dot |
| CN106854620A (en) * | 2017-01-22 | 2017-06-16 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of rapidly and efficiently detection total plate count and preparation method thereof |
| CN108410691A (en) * | 2018-01-18 | 2018-08-17 | 浙江工商大学 | The application of electrospun fibers film and microorganism testing slice containing the tunica fibrosa |
| CN111088156A (en) * | 2020-01-06 | 2020-05-01 | 山东省海洋资源与环境研究院 | Coliform group testing piece for marine environment monitoring and accessory device used in cooperation with coliform group testing piece |
| WO2021022750A1 (en) * | 2019-08-06 | 2021-02-11 | 李晓 | Test piece |
-
2014
- 2014-05-13 CN CN201420242964.2U patent/CN203833945U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754849A (en) * | 2016-03-31 | 2016-07-13 | 吉林农业大学 | Total bacterial count testing slip and preparation method and application thereof |
| CN105754849B (en) * | 2016-03-31 | 2018-02-16 | 吉林农业大学 | A kind of total plate count test piece, preparation method and applications |
| CN106802290A (en) * | 2016-11-29 | 2017-06-06 | 武汉市宇驰检测技术有限公司 | A kind of fluorescence spectrophotometry that E. CoIi content is detected based on carbon quantum dot |
| CN106520542A (en) * | 2016-12-16 | 2017-03-22 | 天津科技大学 | Total bacterial count test piece and manufacturing method thereof |
| CN106854620A (en) * | 2017-01-22 | 2017-06-16 | 贵州勤邦食品安全科学技术有限公司 | A kind of test piece of rapidly and efficiently detection total plate count and preparation method thereof |
| CN108410691A (en) * | 2018-01-18 | 2018-08-17 | 浙江工商大学 | The application of electrospun fibers film and microorganism testing slice containing the tunica fibrosa |
| WO2021022750A1 (en) * | 2019-08-06 | 2021-02-11 | 李晓 | Test piece |
| CN111088156A (en) * | 2020-01-06 | 2020-05-01 | 山东省海洋资源与环境研究院 | Coliform group testing piece for marine environment monitoring and accessory device used in cooperation with coliform group testing piece |
| CN111088156B (en) * | 2020-01-06 | 2021-03-30 | 山东省海洋资源与环境研究院 | Coliform group testing piece for marine environment monitoring and accessory device used in cooperation with coliform group testing piece |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140917 Termination date: 20210513 |