CN203748527U - Cell freezing container - Google Patents

Cell freezing container Download PDF

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Publication number
CN203748527U
CN203748527U CN201420108418.XU CN201420108418U CN203748527U CN 203748527 U CN203748527 U CN 203748527U CN 201420108418 U CN201420108418 U CN 201420108418U CN 203748527 U CN203748527 U CN 203748527U
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CN
China
Prior art keywords
test tube
box
freezing container
cell freezing
sealing surface
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN201420108418.XU
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Chinese (zh)
Inventor
刘清泉
章新泉
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Hangzhou Ke Mosi Science And Technology Ltd
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Hangzhou Ke Mosi Science And Technology Ltd
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Priority to CN201420108418.XU priority Critical patent/CN203748527U/en
Application granted granted Critical
Publication of CN203748527U publication Critical patent/CN203748527U/en
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Expired - Lifetime legal-status Critical Current

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Abstract

The utility model relates to a device for freezing stored cells. A cell freezing container comprises a box, wherein a test tube storage rack and a heat storage block are arranged in the box; the box comprises a bottom box and an upper cover which are formed by insulating materials; the heat storage block is formed by metal materials; support legs are arranged on the outer surface of the bottom wall of the bottom box and have an annular structure; air pores communicating inner space and outer space are formed in the top wall of the upper cover and the bottom wall of the bottom box. The utility model provides the cell freezing container without adding liquid-state buffer medium, and solves the problem that the liquid-state buffer medium needs to be added into the existing cell freezing container.

Description

Cell freezing container
Technical field
The utility model relates to for storing cell and carries out freezing device, relates in particular to a kind of cell freezing container.
Background technology
If being carried out to freezing rate when freezing, cell all can cause cell to lose activity too soon or too slowly, most cells is carried out when freezing to the freezing rate of cell and be conventionally controlled at the speed of 1 DEG C/min and lower the temperature, but it is also the same not to the utmost that different cells is carried out when freezing to freezing quick-frozen.
Current known cell cryopreservation box is all plastic casing, and liner is made with foam in inside, adds isopropyl alcohol to do the structure of buffer medium.Be 2011104362120 in Chinese Patent Application No., within open day, be July 18 in 2012 day, be called in the patent document of " a kind of multipurpose cell freezing and frozen rear recovery container " and disclose a kind of cell freezing container.Existing cell freezing container has the following disadvantages: at ultra low temperature freezer (being generally-80 DEG C) if in use in the tactile container that directly catches, easily cause frostbite; Isopropyl alcohol is in use volatile, regularly add new isopropyl alcohol can continue to use, and the long-term isopropyl alcohol consuming has caused a large amount of wastings of resources and pollution to a certain degree.
Utility model content
The utility model provides a kind of cell freezing container without adding liquid buffer medium, has solved the problem that existing cell freezing container need to add liquid buffer memory medium.
Above technical problem solves by following technical proposal: a kind of cell freezing container, comprise box, described cassette interior is provided with test tube storage rack and heat storage blocks, described box comprises end box and upper cover, box of the described end and upper cover are made by heat-insulating material, and described heat storage blocks is made by metal material, and the diapire outer surface of box of the described end is provided with feet, described feet is loop configuration, and the diapire of the roof of described upper cover and end box is all provided with the pore that is communicated with space, box inside and outside.When use, cell leaves cell in and deposits in pipe, then cell is deposited to pipe is placed in test tube storage rack, then by upper cover lid on earth on box, finally the utility model is put in the freezing equipments such as refrigerator, carry out freezing.Controlling the cold air amount in box that enters by the control size of heat storage blocks and the size of pore meets the requirements the freezing rate of cell.
As preferably, the upper end of box of the described end is provided with the annular lower sealing surface of circumferentially extending along end box, the bottom of described upper cover is provided with the upper sealing surface that same lower sealing surface coordinates, and described lower sealing surface is provided with the annular ice sealing ring circumferentially extending along lower sealing surface and forms groove.Use procedure this, air leakage difference when if lid covers on end box,, actual freezing rate can be different with the deviation of design freezing rate, when deviation is large, can not meet freezing requirement, for the ease of control deviation, upper lid and bottom box design is tightly connected better, if and by tight fit with rubber seal is set seals, box folding not how many times will produce poor sealing phenomenon.This technical scheme forms groove by ice sealing ring is set, when use, form in groove and fill water at ice sealing ring, in the time that temperature drops to freezing point, water freezing is sealed connected together at the bottom of by upper cover and box, then continues to cool to the freezing rate can be with design time of freezing rate when subzero identical.Therefore not only seal convenient but also long service life.
As preferably, between described upper sealing surface and lower sealing surface, be also provided with some negative pressure shape coelosis.In refrigerating process, the gas temperature in negative pressure shape coelosis declines and air pressure decline, and box junction is inside and outside produces draught head, thereby plays the effect that improves the sealing effectiveness between upper lid and bottom box.
As preferably, described negative pressure shape coelosis is the loop configuration of circumferentially extending along described lower sealing surface.Whole circumferential sealing effectiveness to sealing surface can both play good castering action.
As preferably, all described negative pressure shape chambeies are all positioned at described ice sealing ring and form inside or the outside in the space that groove surrounds, the sidewall that described ice sealing ring forms the close described negative pressure shape coelosis of groove is provided with the annular flange flange that forms the extension of groove bearing of trend along described ice sealing ring, and described upper sealing surface is provided with clasp joint in the annular barb of extending along described ice sealing ring formation groove bearing of trend of described flange.Can either be effectively anti-stagnant ice sealing ring form in the process that water freezing in groove forms ice sealing ring and cause negative pressure shape coelosis can not form negative pressure upper cover jack-up; Can also improve the convenience in the time that ice sealing ring forms water filling in groove, produce bonding because can not expand into same upper cover contact when water is too shallow in ice sealing ring formation groove, and easily diffuse out cassette interior while pouring water into more, this technical scheme can make not need water to fill, and when being extend into ice sealing ring and formed in groove by barb, liquid level rises and reaches the water surface and meet the requirements.
As preferably, in described pore, be provided with the adjustable valve of aperture, described valve comprises the spool fixed mount that is connected in the valve seat of pore one end and is connected in the pore other end and the ejector sleeve that drives spool fixed mount to move towards described valve seat, described ejector sleeve is threaded in described pore, described valve seat is provided with at least two valve openings, described spool fixed mount is provided with and drives the open valve spring of spool fixed mount away from described valve seat, and described spool fixed mount is provided with quantity with the identical spool correspondingly described valve opening being sealed of described valve opening quantity; All spools all do not equate with the distance of corresponding valve opening.Can change by changing the quantity of valve opening unlatching the aperture of valve, to change freezing rate.The method of the opening value of this kind of change valve also has following beneficial effect: a kind of equidistant spool and valve opening coordinate while producing wearing and tearing, can not cause all opening value control to be lost efficacy.
As preferably, described test tube storage rack is provided with some test tubes and deposits hole, described test tube is deposited the supporting rod that is provided with at least three along continuous straight runs extensions in hole, described supporting rod can be connected in test tube by clamp springs horizontal extension and deposit hole, and the center of circle that the bare terminal end of described supporting rod is deposited the polygonal circumscribed circle that is projected as summit of hole depth direction along test tube is positioned at the bare terminal end of described supporting rod the is deposited hole depth direction polygonal inside that is projected as summit along described test tube.When use, test tube is left in test tube and deposits in hole along circumferentially clamping by supporting rod, can all keep to the test tubes of different sizes that sidewall large area is unsettled to be deposited, thereby not only versatility is good but also can make test tube cooling rate everywhere evenly keep better the activity of cell.
As preferably, described test tube is deposited and in hole, is provided with inner sleeve, described inner sleeve comprises epimere and hypomere, described epimere hangs on rotationally described test tube and deposits hole, described hypomere is threadedly connected to described epimere lower end, described hypomere and test tube are deposited between hole and are provided with along test tube and deposit the anti-rotation block that hole depth the direction chute extending and the prevention hypomere being inserted in chute rotate, described hypomere is provided with thimble, and described thimble is positioned at the bare terminal end of described supporting rod the is deposited hole depth direction polygonal inside that is projected as summit along described test tube.When use, thimble withstands on the bottom of test tube test tube is supported.Can all keep to the test tube of differing heights that diapire large area is unsettled to be deposited, thus not only versatility good and also can further improve test tube everywhere the uniformity of cooling rate keep better the activity of cell.
As preferably, described thimble upper end is provided with sucker.Can effectively prevent test tube landing from thimble, good connecting effect.
As preferably, the bare terminal end of described supporting rod is provided with rotating fulcrum ball.Can either make test tube with being some contact between supporting rod, the unsettled area that point contacts test tube is large, and cooling rate is evenly better; Again can be anti-scratch test tube in the process of stop contact and mobile test tube.
The utlity model has following advantage: without adding liquid buffer memory medium, pollution-free, lossless, environmental protection and energy saving; Upper cover and base are all made of heat-insulating material, can directly catch tactile under low temperature condition; Easy to use, lightweight; Heat storage blocks is set, can cushions variations in temperature; Pore is set, is convenient to control variations in temperature.
Brief description of the drawings
Fig. 1 is the cross-sectional schematic of the utility model embodiment mono-.
Fig. 2 is the cross-sectional schematic of the utility model embodiment bis-.
Fig. 3 is the local enlarged diagram at the A place of Fig. 2.
Fig. 4 is the structural representation of the pore of the utility model embodiment tri-.
Fig. 5 is the schematic diagram of the test tube storage rack of embodiment tri-.
Fig. 6 is the local enlarged diagram at the B place of Fig. 5.
Fig. 7 is the local enlarged diagram at the C place of Fig. 5.
Fig. 8 be inner sleeve and be connected to its upper-part deposit the schematic diagram of the downward projection of hole depth direction along test tube.
In figure: box 1, end box 11, the first support level 111, the second support level 112, feet 113, lower sealing surface 114, ice sealing ring forms groove 115, flange 116, upper cover 12, connect tongue 121, upper sealing surface 122, barb 123, test tube storage rack 2, test tube is deposited hole 21, inner sleeve 22, supporting rod 23, thimble 24, chute 25, anti-rotation block 26, fulcrum ball 27, sucker 28, clamp springs 29, heat storage blocks 3, pore 4, negative pressure shape coelosis 5, valve 6, valve seat 61, spool fixed mount 62, ejector sleeve 63, valve opening 64, spool 65, open valve spring 66, the bare terminal end of supporting rod is deposited the tetragonal circumscribed circle 7 that is projected as summit of hole depth direction along test tube, the bare terminal end of supporting rod is deposited the quadrangle that is projected as summit 8 of hole depth direction along test tube.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the utility model is further described.
Embodiment mono-, referring to Fig. 1, a kind of cell freezing container, comprises box 1, test tube storage rack 2, heat storage blocks 3 and pore 4.
Box 1 comprises end box 11 and covers the upper cover 12 on end box.End box 11 is made by heat-insulating material.End box 11 is shell-like structure.The sidewall of end box 11 is provided with the first support level 111 and the second support level 112.The first support level 111 is positioned at the top of the second support level 112.The diapire outer surface of end box 11 is provided with feet 113.Feet 113 is loop configuration.
Upper cover 12 is made by heat-insulating material.Upper cover 12 is shell-like structure.Upper cover 12 is provided with annular and connects tongue 121.Connecting tongue 121 is sealed connected together with tight fit between the sidewall of the first support level 111.
Test tube storage rack 2 is provided with some test tubes and deposits hole 21.Test tube storage rack 2 is shelved on the second support level 112.
Heat storage blocks 3 is shelved on the diapire of end box 11.Heat storage blocks 3 is made by metal material.
Pore 4 has two.A pore in two pores 4 is arranged on the roof of upper cover 12, the diapire that another pore is arranged on end box 11.Pore 4 is for being communicated with the space, inside and outside of box 1.
When use, take off upper cover 12, the test tube that the test tube of depositing cell is placed in test tube storage rack 2 is correspondingly deposited in hole 21, then covers upper cover 12.The utility model is put in low temperature refrigerator and carries out freezingly to existing cell to deposit cell in pipe, and cell lowers the temperature according to the definite rate of temperature fall of the utility model.When freezing in the present embodiment, the rate of temperature fall of cell is 1 DEG C/min.
Embodiment bis-, with the difference of embodiment mono-is:
Referring to Fig. 2, the upper end of end box 11 is provided with the annular lower sealing surface 114 of circumferentially extending along end box.Lower sealing surface 114 is provided with the annular ice sealing ring circumferentially extending along lower sealing surface and forms groove 115.The position of the corresponding lower sealing surface in bottom of upper cover 12 is provided with sealing surface 122.Between upper sealing surface 112 and lower sealing surface 113, be provided with negative pressure shape coelosis 5.Negative pressure shape coelosis 5 only has one.Negative pressure shape coelosis 5 is the loop configuration of circumferentially extending along lower sealing surface.Negative pressure shape chamber 122 is positioned at ice sealing ring and forms the inside (being certainly positioned at outside is also fine) in the space that surrounds of groove 115, negative pressure shape coelosis 5 can be also multiple, also can for other than ring type structure, form as long as all negative pressure shape coelosis are positioned at ice sealing ring simultaneously interior volume or the outside that groove 115 surrounds.
Referring to Fig. 3, the madial wall that ice sealing ring forms groove 115 is provided with the annular flange flange 116 that forms the extension of groove bearing of trend along ice sealing ring.Upper sealing surface 122 is provided with clasp joint in the barb 123 of flange 116.Barb 123 is to form along ice sealing ring the annular that groove bearing of trend extends.
When use, referring to Fig. 2, take off upper cover 12, the test tube that the test tube of depositing cell is placed in test tube storage rack 2 is correspondingly deposited in hole 21, form in groove 115 and pour water at ice sealing ring, then cover upper cover 12 to barb 123 clasp joints on flange 116.The utility model is put in low temperature refrigerator and carries out freezingly to existing cell to deposit cell in pipe, and cell lowers the temperature according to the definite rate of temperature fall of the utility model.In refrigerating process, ice sealing ring forms the water freezing in groove 115 and forms ice sealing ring upper cover 12 and end box 11 are sealed connected together.Ice sealing ring also plays the effect of further barb 123 clasp joints being locked at flange 116 simultaneously.Air pressure in negative pressure shape coelosis 5, along with temperature reduces and lower general, plays again the effect of the connection sealing effectiveness of further raising upper cover 12 and end box 11.
Embodiment tri-, with the difference of embodiment bis-is:
Referring to Fig. 4, in pore 4, be provided with the adjustable valve of aperture 6.Valve 6 comprises the valve seat 61, spool fixed mount 62 and the ejector sleeve 63 that distribute successively along pore bearing of trend.The wall portion of valve seat 61 and pore 4 is sealed connected together with the form of integrative-structure.Valve seat 61 is provided with 6 valve holes 64.Valve opening quantity and aperture area sum in each valve hole all equate.Spool fixed mount 62 is provided with 6 valve cores 65.Spool quantity in each valve core 65 equates with the quantity of the valve opening in corresponding row.Spool fixed mount 62 is supported in pore 4 by opening valve spring 66.Ejector sleeve 63 is threaded in pore 4.Spool in same valve core with the distance between corresponding valve opening equate, spool in same row is unequal with the distance of corresponding valve opening with the spool in distance and another row of corresponding valve opening.
The aperture of control valve taking the method that changes the aperture area of pore and realize freezing rate as: in the time that all spools 65 do not block valve opening 64, it is maximum that the aperture of valve 6 reaches, rotating ejector sleeve 63 makes ejector sleeve 63 near valve seat 61, ejector sleeve 63 promotes spool fixed mount 62 near valve seat 61, spool fixed mount 62 is with movable valve plug 65 near valve seat 61, open valve spring 66 and receive extruding and energy storage, in 6 valve cores 65, being arranged in a rightmost valve core is first inserted into 6 valve holes 64 and is arranged in a rightmost valve hole and this valve hole is sealed up, the aperture of valve 6 reduces a setting value, another valve core that continues to decline is inserted into and in another valve hole, makes the aperture of valve 6 setting value that declines again, so analogize, in the time that all spools are all inserted in all valve openings, valve 6 is fully closed.Make it increase if rotate ejector sleeve 64, opening under the effect of valve spring 66, spool fixed mount 62 can rise and away from valve seat 61.
Referring to Fig. 5, test tube is deposited and in hole 21, is provided with inner sleeve 22, supporting rod 23 and thimble 24.
Inner sleeve 22 comprises epimere 221 and hypomere 222.Epimere 221 hangs on rotationally test tube and deposits hole 21.Hypomere 222 is threadedly connected to epimere 221 lower ends.Hypomere 222 and test tube are deposited between hole 21 and are provided with along test tube and deposit the anti-rotation block 26 that hole depth the direction chute 25 extending and the prevention hypomere being inserted in chute rotate.Chute 25 is arranged at test tube and deposits the hole wall in hole 21.Anti-rotation block 26 is arranged at hypomere 222.Supporting rod 23 along continuous straight runs extend.Supporting rod 23 is arranged in epimere 221.Supporting rod 23 and test tube are deposited and between hole 21, are provided with clamp springs 29.Clamp springs 29 is for making supporting rod 23 stretch towards epimere 221 inside.Thimble 24 is arranged at the diapire of hypomere 222.Thimble 24 vertically extends.
Referring to Fig. 6, the bare terminal end of supporting rod 23 is provided with rotating fulcrum ball 27.The bare terminal end of supporting rod refers to while use that supporting rod is with one end of test tube contact.
Referring to Fig. 7, thimble 24 upper ends are provided with sucker 28.
Referring to Fig. 8, supporting rod 23 has four.The center of circle that the bare terminal end of supporting rod 23 is deposited the tetragonal circumscribed circle 7 that is projected as summit of hole depth direction along test tube is positioned at the bare terminal end of supporting rod is deposited the quadrangle that is projected as summit 8 of hole depth direction inside along test tube.Thimble 24 is positioned at the bare terminal end of supporting rod is deposited the quadrangle that is projected as summit 8 of hole depth direction inside along test tube.The bare terminal end of supporting rod the is deposited hole depth direction quadrangle that is projected as summit 8 along test tube is square.
Arrive Fig. 8 referring to Fig. 5, the method that test tube is fixed is: remove to rotate epimere 221 according to the height that is stored test tube and make height that hypomere 222 rises to thimble 24 to needed position, the diapire of test tube is shelved on thimble 24 and by sucker 28 and adsorbs and prevent landing.Three supporting rods 23 are clamped in the sidewall of test tube under the effect of clamp springs 29 with test tube contact by fulcrum ball 27.
Embodiment tetra-is that supporting rod only has three with the difference of embodiment tri-.The center of circle that the bare terminal end of supporting rod is deposited the circumscribed circle at the projection place of hole depth direction along test tube is positioned at the bare terminal end of supporting rod the is deposited hole depth direction leg-of-mutton inside that is projected as summit along test tube.Thimble is positioned at the bare terminal end of supporting rod the is deposited hole depth direction leg-of-mutton inside that is projected as summit along test tube.The triangle that is projected as summit that the bare terminal end of supporting rod is deposited hole depth direction along test tube is equilateral triangle.

Claims (10)

1. a cell freezing container, it is characterized in that, comprise box, described cassette interior is provided with test tube storage rack and heat storage blocks, described box comprises end box and upper cover, and box of the described end and upper cover are made by heat-insulating material, and described heat storage blocks is made by metal material, the diapire outer surface of box of the described end is provided with feet, and the diapire of the roof of described upper cover and end box is all provided with the pore that is communicated with space, box inside and outside.
2. a kind of cell freezing container according to claim 1, it is characterized in that, the upper end of box of the described end is provided with the annular lower sealing surface of circumferentially extending along end box, the bottom of described upper cover is provided with the upper sealing surface that same lower sealing surface coordinates, and described lower sealing surface is provided with the annular ice sealing ring circumferentially extending along lower sealing surface and forms groove.
3. a kind of cell freezing container according to claim 2, is characterized in that, is also provided with some negative pressure shape coelosis between described upper sealing surface and lower sealing surface.
4. a kind of cell freezing container according to claim 3, is characterized in that, described negative pressure shape coelosis is the loop configuration of circumferentially extending along described lower sealing surface.
5. according to a kind of cell freezing container described in claim 3 or 4, it is characterized in that, all described negative pressure shape chambeies are all positioned at described ice sealing ring and form inside or the outside in the space that groove surrounds, the sidewall that described ice sealing ring forms the close described negative pressure shape coelosis of groove is provided with the annular flange flange that forms the extension of groove bearing of trend along described ice sealing ring, and described upper sealing surface is provided with clasp joint in the annular barb of extending along described ice sealing ring formation groove bearing of trend of described flange.
6. according to a kind of cell freezing container described in claim 1 or 2 or 3 or 4, it is characterized in that, in described pore, be provided with the adjustable valve of aperture, described valve comprises the valve seat that is connected in pore one end, and be connected in the spool fixed mount of the pore other end and the ejector sleeve that drives spool fixed mount to move towards described valve seat, described ejector sleeve is threaded in described pore, described valve seat is provided with at least two valve openings, described spool fixed mount is provided with and drives the open valve spring of spool fixed mount away from described valve seat, described spool fixed mount is provided with quantity with the identical spool correspondingly described valve opening being sealed of described valve opening quantity, all spools all do not equate with the distance of corresponding valve opening.
7. according to a kind of cell freezing container described in claim 1 or 2 or 3 or 4, it is characterized in that, described test tube storage rack is provided with some test tubes and deposits hole, described test tube is deposited the supporting rod that is provided with at least three along continuous straight runs extensions in hole, described supporting rod can be connected in test tube by clamp springs horizontal extension and deposit hole, and the center of circle that the bare terminal end of described supporting rod is deposited the polygonal circumscribed circle that is projected as summit of hole depth direction along test tube is positioned at the bare terminal end of described supporting rod the is deposited hole depth direction polygonal inside that is projected as summit along described test tube.
8. a kind of cell freezing container according to claim 7, it is characterized in that, described test tube is deposited and in hole, is provided with inner sleeve, described inner sleeve comprises epimere and hypomere, described epimere hangs on rotationally described test tube and deposits hole, described hypomere is threadedly connected to described epimere lower end, described hypomere and test tube are deposited between hole and are provided with along test tube and deposit the anti-rotation block that hole depth the direction chute extending and the prevention hypomere being inserted in chute rotate, described hypomere is provided with thimble, described thimble is positioned at the bare terminal end of described supporting rod the is deposited hole depth direction polygonal inside that is projected as summit along described test tube.
9. a kind of cell freezing container according to claim 8, is characterized in that, described thimble upper end is provided with sucker.
10. a kind of cell freezing container according to claim 7, is characterized in that, the bare terminal end of described supporting rod is provided with rotating fulcrum ball.
CN201420108418.XU 2014-03-12 2014-03-12 Cell freezing container Expired - Lifetime CN203748527U (en)

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Application Number Priority Date Filing Date Title
CN201420108418.XU CN203748527U (en) 2014-03-12 2014-03-12 Cell freezing container

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Application Number Priority Date Filing Date Title
CN201420108418.XU CN203748527U (en) 2014-03-12 2014-03-12 Cell freezing container

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103988835A (en) * 2014-03-12 2014-08-20 杭州科默斯科技有限公司 Cell freezing box
CN108124852A (en) * 2017-12-23 2018-06-08 郑州仁宏医药科技有限公司 A kind of multiple damping organ transplant transfer device
CN108142411A (en) * 2017-12-23 2018-06-12 郑州仁宏医药科技有限公司 A kind of organ transplant transfer device
CN109997841A (en) * 2019-04-09 2019-07-12 上海交通大学 Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere
CN111919838A (en) * 2020-09-03 2020-11-13 林彩琴 Utilize fixed cell cryopreserving cooling box of atmospheric pressure

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103988835A (en) * 2014-03-12 2014-08-20 杭州科默斯科技有限公司 Cell freezing box
CN108124852A (en) * 2017-12-23 2018-06-08 郑州仁宏医药科技有限公司 A kind of multiple damping organ transplant transfer device
CN108142411A (en) * 2017-12-23 2018-06-12 郑州仁宏医药科技有限公司 A kind of organ transplant transfer device
CN109997841A (en) * 2019-04-09 2019-07-12 上海交通大学 Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere
CN109997841B (en) * 2019-04-09 2021-02-02 上海交通大学 Human gamete rapid freezing and thawing visualization environment cabin maintaining atmospheric air atmosphere
CN111919838A (en) * 2020-09-03 2020-11-13 林彩琴 Utilize fixed cell cryopreserving cooling box of atmospheric pressure

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Granted publication date: 20140806