CN203732482U - Nanozinc-reinforced olfaction tissue biosensor detection device - Google Patents
Nanozinc-reinforced olfaction tissue biosensor detection device Download PDFInfo
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- CN203732482U CN203732482U CN201420031859.4U CN201420031859U CN203732482U CN 203732482 U CN203732482 U CN 203732482U CN 201420031859 U CN201420031859 U CN 201420031859U CN 203732482 U CN203732482 U CN 203732482U
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Abstract
The utility model discloses a nanozinc-reinforced olfaction tissue biosensor detection device which is composed of a perfusion system, a biological detection system and a data acquisition and storage system, wherein the perfusion system and the data acquisition and storage system are respectively connected with the biological detection system; the perfusion system is composed of an oxygen tank, an oxygen inlet channel, a sample inlet system and a sample outlet system; the biological detection system is composed of an olfaction tissue biosensor, a sensor fixing and insulation system and a temperature controller; and the data acquisition and storage system is composed of a multichannel amplifier, a data acquisition card and a computer. The detection device has the characteristics of simple preparation process, wide application range, high signal-to-noise ratio and the like, is convenient for detection, does not need fixation, can be widely used in the field related to smell detection, and provides an effective detection means for research of the biological olfaction sensation molecular mechanism.
Description
Technical field
The utility model relates to a kind of odor detection intensifier of biology sensor, relates in particular to the sense of smell tissue biological sensor detecting device that a kind of Nano-Zinc strengthens.
Background technology
Sense of smell tissue biological sensor is to be combined to detect a kind of biology sensor of extraneous Odor stimulation with physics or chemical sensor from the complete olfactory mucosa of peeling off of rat nasal cavity, there is the advantages such as method is simple, biological function reservation is complete, the response time is short, there is significant application value in fields such as biomedicine, environmental monitoring, food industry.But the factors such as the noise that when noise of intrinsic noise, olfactory mucosa and the device coupled interface of senser element and test, external environment interference produces can reduce its signal to noise ratio (S/N ratio), limit its detection to low concentration Odor stimulation, hinder its actual effectively application.In mammalian, contain the Nano-Zinc metallic particles of 1-2nm, the outer electric signal of the born of the same parents that produce when olfactory mucosa is subject to Odor stimulation has humidification.Therefore, the sensing device that Nano-Zinc is combined with sense of smell tissue biological sensor, prepare the bio-sensing detection means simple, easy to detect, signal to noise ratio (S/N ratio) is high as a kind of, for sense of smell tissue biological sensor provides device and method to effective detection and the identification of low concentration Odor stimulation.
Summary of the invention
The purpose of this utility model is for the deficiencies in the prior art, the sense of smell tissue biological sensor detecting device that provides a kind of Nano-Zinc to strengthen.
The purpose of this utility model is achieved through the following technical solutions: the sense of smell tissue biological sensor detecting device that a kind of Nano-Zinc strengthens, it is made up of perfusion system, biological detection system and data acquisition and storage system three parts, and perfusion system is all connected with biological detection system with data acquisition and storage system; Wherein, perfusion system by oxygen tank, enter oxygen path, sampling system and go out sample system to form, biological detection system is fixed by sense of smell tissue biological sensor, sensor and heat-insulation system and temperature controller form, and data acquisition and storage system are made up of multichannel pmultiple amplifier, data collecting card and computer;
In described perfusion system, sampling system comprises the first sample cell, the second sample cell, the first peristaltic pump, the second peristaltic pump and two-way sample introduction path, goes out sample system and comprises sample path, the 3rd peristaltic pump and waste liquid tank; The input end that enters oxygen path connects oxygen tank, and two output terminals insert respectively the first sample cell and the second sample cell; Two input ends of two-way sample introduction path also insert respectively in the first sample cell and the second sample cell, its output terminal is the test chamber top of adherent insertion sense of smell tissue biological sensor, on two input ends of two-way sample introduction path, the first peristaltic pump and the second peristaltic pump are installed respectively, go out sample path input end the test chamber bottom of the also adherent insertion sense of smell of the 3rd peristaltic pump tissue biological sensor has been installed, output terminal connects waste liquid tank;
In described biological detection system, sense of smell tissue biological sensor is made up of olfactory mucosa and microelectrode array sensor, and sensor is fixed and heat-insulation system is made up of top board and heatable soleplate; Olfactory mucosa cilium towards be above laid in microelectrode array sensor surface and with the coupling of microelectrode array sensor generation capacitance-resistance, microelectrode array sensor is arranged in 8 × 8 square formations by 64 gold electrodes and forms; Utilize sensor to fix and the top board of heat-insulation system is fixed on sense of smell tissue biological sensor in heatable soleplate, and be used in conjunction with transmission of signal by the phase of solder joint on metal probe and microelectrode array sensor on top board, heatable soleplate connects temperature controller outward;
In described data acquisition and storage system, multichannel pmultiple amplifier and sense of smell tissus sensor are altogether, and the data input pin connecting sensor of multichannel pmultiple amplifier is fixed and the data acquisition serial ports of the top board of heat-insulation system, data output end connection data capture card, data collecting card is connected with computer by USB serial ports again.
The beneficial effects of the utility model are that the sense of smell tissue biological sensor detecting device that provides a kind of Nano-Zinc to strengthen, has realized the effective detection to Odor stimulation.There is simple, easy to detect, applied widely, the signal to noise ratio (S/N ratio) high of preparation, and without fixing, to strengthen effect adjustable flexibly, can be widely used in the association area of odor detection, the research of simultaneously also experiencing molecular mechanism for Biologic Olfaction provides effective detection means.
Brief description of the drawings
Fig. 1 is the composition schematic diagram that the utility model Nano-Zinc strengthens sense of smell tissue biological sensor device for testing smell;
Fig. 2 is the sense of smell tissue biological sensor composition of the utility model device and detects principle schematic;
In figure: oxygen tank 1, enter oxygen path 2, sampling system 3, the first sample cell 31, the second sample cell 32, the first peristaltic pump 33, the second peristaltic pump 34, two-way sample introduction path 35, go out sample system 4, go out sample path 41, the 3rd peristaltic pump 42, waste liquid tank 43, sense of smell tissue biological sensor 5, olfactory mucosa 51, olfactory receptor cell 52, microelectrode array sensor 53, gold electrode 54, sensor is fixed and heat-insulation system 6, metal probe 61, heatable soleplate 62, temperature-controlling system 7, multichannel amplifier system 8, data collecting card 9, computer 10.
Embodiment
Below in conjunction with drawings and the specific embodiments, the utility model is described in detail, but is not restriction the utility model.
Be illustrated in figure 1 the device design that the utility model Nano-Zinc strengthens sense of smell tissue biological sensor odor detection, whole device is made up of perfusion system, biological detection system and data acquisition and storage system three parts, and perfusion system is all connected with biological detection system with data acquisition and storage system.Wherein, perfusion system by oxygen tank 1, enter oxygen path 2, sampling system 3 and go out sample system 4 to form; Biological detection system is fixed by sense of smell tissue biological sensor 5, sensor and heat-insulation system 6 and temperature controller 7 form; Data acquisition and storage system are made up of multichannel pmultiple amplifier 8, data collecting card 9 and computer 10.
In perfusion system, sampling system 3 comprises the first sample cell 31, the second sample cell 32, the first peristaltic pump 33, the second peristaltic pump 34 and two-way sample introduction path 35; Comprise sample path 41, the 3rd peristaltic pump 42 and waste liquid tank 43 and go out sample system 4.The input end that enters oxygen path 2 connects 1, two output terminal of oxygen tank and inserts respectively the first sample cell 31 and the second sample cell 32.Simultaneously, in sampling system 3, two input ends of two-way sample introduction path 35 also insert respectively in the first sample cell 31 and the second sample cell 32, its output terminal is the test chamber top of adherent insertion sense of smell tissue biological sensor 5, and the first peristaltic pump 33 and the second peristaltic pump 34 have been installed respectively on two input ends of two-way sample introduction path 35.On the other hand, go out the test chamber bottom that outes sample path 41 input ends the 3rd peristaltic pump 42 adherent insertion sense of smell tissue biological sensor 5 have been installed of sample system 4, output terminal connects waste liquid tank 43.
In biological detection system, sense of smell tissue biological sensor 5 is made up of olfactory mucosa 51 and microelectrode array sensor 53, as shown in Figure 2; And sensor is fixed and heat-insulation system 6 is made up of top board 61 and heatable soleplate 63.By olfactory mucosa 51 ciliums towards the surface that is above laid in microelectrode array sensor 53.Microelectrode array sensor 53 adopts standard miromaching to make, and the diameter of the gold electrode 54 distributing on it is 30 μ m, electrode separation 100 μ m, and 64 gold electrodes 54 are arranged in 8 × 8 square formations.Utilize sensor to fix and the top board 61 of heat-insulation system 6 is fixed on sense of smell tissue biological sensor 5 in heatable soleplate 63, and be connected with the solder joint on microelectrode array sensor 53 with conducting electrical signals to data acquisition serial ports by the metal probe 62 on top board 61, the outer company of heatable soleplate 63 temperature controller 7.Fix in backward sense of smell tissue biological sensor 5 perfusion PBS damping fluid and detach fast, leaving standstill and make olfactory mucosa 51 and microelectrode array sensor 53 that capacitance-resistance coupling occur, completing the assembling of sense of smell tissue biological sensor with fixing.
In data acquisition and storage system, multichannel pmultiple amplifier 8 and sense of smell tissus sensor 5 are altogether, and the data input pin connecting sensor of multichannel pmultiple amplifier 8 is fixed and the data acquisition serial ports of the top board 61 of heat-insulation system 6, data output end connection data capture card 9, data collecting card 9 is connected with computer 10 by USB serial ports again.
Claims (1)
1. the sense of smell tissue biological sensor detecting device that Nano-Zinc strengthens, it is characterized in that, it is made up of perfusion system, biological detection system and data acquisition and storage system three parts, and perfusion system is all connected with biological detection system with data acquisition and storage system;
Wherein, perfusion system by oxygen tank (1), enter oxygen path (2), sampling system (3) and go out sample system (4) to form, biological detection system by sense of smell tissue biological sensor (5), sensor fixes and heat-insulation system (6) and temperature controller (7) form, data acquisition and storage system are made up of multichannel pmultiple amplifier (8), data collecting card (9) and computer (10); In described perfusion system, sampling system (3) comprises the first sample cell (31), the second sample cell (32), the first peristaltic pump (33), the second peristaltic pump (34) and two-way sample introduction path (35), goes out sample system (4) and comprises sample path (41), the 3rd peristaltic pump (42) and waste liquid tank (43); The input end that enters oxygen path (2) connects oxygen tank (1), and two output terminals insert respectively the first sample cell (31) and the second sample cell (32); Two input ends of two-way sample introduction path (35) also insert respectively in the first sample cell (31) and the second sample cell (32), its output terminal is the test chamber top of adherent insertion sense of smell tissue biological's sensor (5), the first peristaltic pump (33) and the second peristaltic pump (34) have been installed respectively on two input ends of two-way sample introduction path (35), go out sample path (41) input end the test chamber bottom of the 3rd peristaltic pump (42) adherent insertion sense of smell tissue biological's sensor (5) has been installed, output terminal connects waste liquid tank (43);
In described biological detection system, sense of smell tissue biological sensor (5) is made up of olfactory mucosa (51) and microelectrode array sensor (53), and sensor fixes and heat-insulation system (6) is made up of top board (61) and heatable soleplate (63); Olfactory mucosa (51) cilium is towards being above laid in the surface of microelectrode array sensor (53) and with microelectrode array sensor (53), capacitance-resistance coupling occurring, and microelectrode array sensor (53) is arranged in 8 × 8 square formations by 64 gold electrodes (54) and forms; Utilize sensor to fix and the top board (61) of heat-insulation system (6) is fixed on sense of smell tissue biological sensor (5) in heatable soleplate (63), and be used in conjunction with transmission of signal the outer temperature controller (7) that connects of heatable soleplate (63) by the metal probe (62) on top board (61) and the phase of solder joint on microelectrode array sensor (53);
In described data acquisition and storage system, multichannel pmultiple amplifier (8) and sense of smell tissus sensor (5) are altogether, and the data input pin connecting sensor of multichannel pmultiple amplifier (8) is fixed and the data acquisition serial ports of the top board (61) of heat-insulation system (6), data output end connection data capture card (9), data collecting card (9) is connected with computer (10) by USB serial ports again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420031859.4U CN203732482U (en) | 2014-01-18 | 2014-01-18 | Nanozinc-reinforced olfaction tissue biosensor detection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420031859.4U CN203732482U (en) | 2014-01-18 | 2014-01-18 | Nanozinc-reinforced olfaction tissue biosensor detection device |
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| CN203732482U true CN203732482U (en) | 2014-07-23 |
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| CN201420031859.4U Withdrawn - After Issue CN203732482U (en) | 2014-01-18 | 2014-01-18 | Nanozinc-reinforced olfaction tissue biosensor detection device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792377A (en) * | 2014-01-18 | 2014-05-14 | 浙江大学 | Device and method for reinforcing smell detection of smell tissue biological sensor by nano zinc |
| CN107831195A (en) * | 2017-10-30 | 2018-03-23 | 上海应用技术大学 | A kind of animal flesh metal doping nano graphene olfactory sensor |
-
2014
- 2014-01-18 CN CN201420031859.4U patent/CN203732482U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792377A (en) * | 2014-01-18 | 2014-05-14 | 浙江大学 | Device and method for reinforcing smell detection of smell tissue biological sensor by nano zinc |
| CN103792377B (en) * | 2014-01-18 | 2015-07-15 | 浙江大学 | Device and method for reinforcing smell detection of smell tissue biological sensor by nano zinc |
| CN107831195A (en) * | 2017-10-30 | 2018-03-23 | 上海应用技术大学 | A kind of animal flesh metal doping nano graphene olfactory sensor |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140723 Effective date of abandoning: 20150715 |
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| RGAV | Abandon patent right to avoid regrant |