CN203672700U - Cerebrospinal fluid precipitator - Google Patents

Abstract

The utility model discloses a cerebrospinal fluid precipitator. The cerebrospinal fluid precipitator comprises a base (1) and is characterized in that the base (1) is integrally formed by upper and lower cylinders, vertical embossing is arranged on the outer wall of the lower cylinder in the height direction, a glass slide opening (3) is formed in the bottom end of the upper cylinder, and a glass slide (4) is arranged at the glass slide opening (3); a base groove (2) is formed inside the base (1), and a rubber pad (5) is arranged in the base groove (2); a specimen precipitation chamber (10) is arranged on the glass slide (4) in the upper cylinder, a small tubular chamber (11) is arranged in the specimen precipitation chamber (10), and an opening (12) is formed in the top end of the specimen precipitation chamber (10); and a nut (6) is arranged outside a cylinder at the lower part of the specimen precipitation chamber (10), and the small tubular chamber (11) is sleeved with a small camber cover (13). According to the cerebrospinal fluid precipitator, the cell collection efficiency is high, missing inspection can be avoided, the classification effect is good, the success rate is high, scattering and leakage are avoided, the pain caused by recollection of specimens to a patient is avoided, and meanwhile, the increase of the workload of doctors is avoided.

Description

A kind of cerebrospinal fluid settling vessel

Technical field:

The utility model relates to pharmacological evaluation equipment technical field, is a kind of cerebrospinal fluid settling vessel specifically; Be mainly used in hospital laboratory.

Background technology:

Cerebrospinal fluid is the colourless transparent liquid mainly being produced by ventricles of the brain choroid plexus, is full of ventricular system, brain pond and cavum subarachnoidale, and its major function has: 1. regulating intracranial pressure power; 2. buffering external force, minimizing concussion; 3. maintain the relatively constant of brain tissue osmotic pressure; 4. play part lymph liquid, can nutrition near brain tissue transport part metabolic product; 5. performance medicine choroid plexus blood-CSF barrier sees through effect.

The meaning of examination of cerebrospinal fluid: diagnosis and the antidiastole tool of examination of cerebrospinal fluid to nervous system inflammatory and SOL has very important significance, especially the antidiastole of viral encephalitis, tubercular meningitis is had to very high clinical value, common laboratory does not reach the condition that virus separates, the recall rate of tubercle bacillus is very low, and the cytological dynamic change of cerebrospinal fluid has high directive significance to diagnosis, detecting of tumour cell has vital effect to the diagnosis of nervous system neoplasm disease.Along with the moving process of medicine generation and medicine being strict with in the research process of pharmacometabonomics and the modernization of Chinese medicine, the research of cerebrospinal fluid also becomes more and more important in pharmacological research.

The classification of examination of cerebrospinal fluid: 1. pressure inspection (inspection of pressure dynamics, final pressure); 2. outward appearance; 3. cytolgical examination; 4. biochemical analysis (albumen, sugar, chloride, bacteriology checking, immunologic test, protein, electrophoretic examinations, enzyme labeled compound assay); 5. routine inspection (colour of solution, transparency inspection, cell count, qualitative test of protein, semi-quantitative test for glucose, bacterium and parasite inspection, cell classification, cerebrospinal fluid total white blood cells); 6. chemical analysis (protein quantification, protein electrophoresis, glucose quantitation, chloride determination); 7. zymetology and immunologic assay (cerebrospinal fluid enzymatic determination, Determination of Immunoglobulin in Cerebrospinal Fluid); 8. other measure (piezometry, gravity test, potential of hydrogen and gas tension mensuration, TRPT, lactic acid quantitative test, glutamine are measured).

The meaning that wherein CSFC checks is as follows: by cerebrospinal fluid cell harvesting and be fixed on microslide and dyeed, see chip technology by optics (200 ~ 400 times) microscope of high power and carry out profile, structure and the classification of cerebrospinal fluid cell one by one, and the overhauling of iuntercellular mutual relationship, understand definitely the pathological characters of CSFC and the confirmation to pathogenic bacteria such as bacterium, fungi or parasites, and easily to the state of an illness and prognosis judgement; The knurls such as tumour, leukaemia and lymthoma (cancer) cell and normal leucocyte or inflammatory cell etc. are strictly distinguished, and prevented the generation of serious sing misdiagnosis and mistreatment clinically; The source book that simultaneously can retain again censorship cerebrospinal fluid cell specimen is for future reference, and prevents that law afterwards from putting to the proof and the defect such as the difficulty of medical events on qualitative.The dynamic chek of CSFC reduces or disappearance, lymphocyte and monocyte count and the two ratio show and improve or normal gradually as cerebrospinal fluid inflammatory cell, points out the state of an illness to improve or recovery from illness; Otherwise, show aggravation or recurrence.And can be drug therapy decision-making objective basis is provided.Cytolgical examination, adult normal cerebrospinal fluid meter leukocyte count 0.01 × 109/(premature and neonate are in 0.03 × 109/L) below L, but polynuclear leucocyte should not exceed 5, is mainly little, medium size lymphocyte.When meninx is irritant or when inflammatory lesion, the lencocyte count of cerebrospinal fluid can increase.Therefore when central nervous system infection venereal disease becomes, have multinuclear or monocytic increasing in various degree; Various brain tumors particularly close on meninx, the ventricles of the brain or pernicious person, also have leukocytic increasing.Therefore cytolgical examination highly significant.

The previous work of the cytolgical examination of cerebrospinal fluid: because cerebrospinal fluid cell need use microscopic examination, microscope can only be observed the material on microslide, therefore these experiments all relate to a groundwork: cerebrospinal fluid cell precipitation to microslide.

At present cerebrospinal fluid cell precipitation is mainly contained to two kinds to the method on microslide: i.e. cytodiagnosis and natural sedimentation method; Traditional centrifugal cell in centrifugal process is all deposited to the bottom of centrifuge tube, crimp mutually between cell, quick cerebrospinal fluid cell damage when centrifugal, cell harvesting rate is low, and cellular morphology changes, classified counting of leucocyte is brought to certain difficulty, and cellular morphology is destroyed again in film-making process, made cell be difficult for identification; For fear of the defect of centrifugal, someone attempts substituting with natural sedimentation method, natural sedimentation method broken cell rate is low, cellular morphology changes little, can improve accuracy and the Cytometric accuracy of cell classification, but natural sedimentation method required time is grown (12 hours), cell destructible, and cerebrospinal fluid liquid conventionally can flow losses on slide.

The solution of the large concentration such as blood can be deposited on microslide with smearing method, but the cell content of cerebrospinal fluid water sample liquid is low, the cell number that the inspection of cerebrospinal fluid direct smear is collected is few, cannot meet inspection requirements, how cerebrospinal fluid cell precipitation to microslide, up to the present there is no satisfied cerebrospinal fluid settling vessel.

Utility model content:

The purpose of this utility model is the deficiency that overcomes above-mentioned prior art, and a kind of cerebrospinal fluid settling vessel is provided, mainly solve that existing centrifugal cell harvesting rate is low, classified counting of leucocyte is difficult, cell is difficult for identification and natural sedimentation method takes time, and length, cell destructible, cerebrospinal fluid liquid are understood the problems such as flow losses on slide.

The technical solution of the utility model is: a kind of cerebrospinal fluid settling vessel, it comprises base, its special character is, described base is in aggregates by upper and lower right cylinder structure, and upper cylinder diameter is less than lower right cylinder, and the two forms boss structure, lower cylindrical outer wall short transverse has vertical embossing, upper cylindrical body outer wall is established external thread, and microslide opening is established in upper right cylinder bottom, establishes microslide at microslide opening part; The inside of base is concentric recessed base groove, establishes rubber blanket in base groove; This mud chamber of bidding on microslide in upper cylinder, inside, sample mud chamber is tubular structure cell, and tubular structure cell and bottom, sample mud chamber form a hollow integrated, and the two is boss structure, and there is opening on top, sample mud chamber; At the upper right cylinder peripheral hardware nut of base, nut outer wall is established the outside lines of the hand of nut, and mud chamber's opening is established in centre, establishes internal whorl on nut inner wall, and on internal whorl and base, the external thread of cylindrical body outer wall matches; At tubular structure cell overcoat cell lid.

Further, described tubular structure cell bottom is smooth smooth.

Compared with the prior art a kind of cerebrospinal fluid settling vessel described in the utility model has following good effect: 1, can guarantee that the shape of cerebrospinal fluid sediment on microslide is circular, this compares the irregularly shaped of conventional natural sedimentation method, can be not undetected; 2, make settling vessel be convenient to dismounting, clean, sterilize and assembling, alleviated operator's working strength; 3, settling vessel can repeatedly use, and is convenient to move, and has saved chemical examination cost; 4, sample is convenient to leave in refrigerator and is refrigerated, and guarantees cell survival rate, reduces cell damage; 5, bottom, sample mud chamber is smooth smooth and have nut to fix, and can realize hermetically sealedly, prevents that sample from spilling; 6,, in operating process, no longer need to use adhesive material, thereby can not cause sample to pollute; 7, cerebrospinal fluid sediment is the circle of 1 centimetre of diameter, is convenient to cell count; 8, microslide is easily fixing, and stressed evenly, not cracky and cause sample to reveal; 9, each parts are sturdy and durable, and repeatable operation is not fragile; 10, collected cell paster firmly, be evenly distributed, light transmission and coloring all very good; The cellular morphology of 11, collecting is complete, so good classification effect; 12, success ratio is high and do not spill, and has avoided Resurvey sample and the misery brought to patient, has so just reduced patient's the cost of seeking medical advice, and has also avoided increasing the weight of of working doctor amount burden simultaneously.

Accompanying drawing explanation:

Fig. 1 is surface structure schematic diagram of the present utility model;

Fig. 2 is that assembly and connection of the present utility model is related to schematic diagram;

Fig. 3 is component structural schematic diagram of the present utility model.

Drawing explanation:

1 base, 2 base grooves, 3 microslide openings, 4 microslides, 5 rubber blankets, 6 nuts, 7 mud chamber's openings, the outside lines of the hand of 8 nuts, 9 internal whorls, 10 sample mud chambers, 11 tubular structure cells, 12 openings, 13 cell lids.

Embodiment:

Contrast accompanying drawing below, by the description to specific embodiment, to embodiment of the present utility model, and effect and principle of work, manufacturing process and the operation using method etc. of mutual alignment between related each component shape, structure, each several part and annexation, each several part, be described in further detail.

Embodiment 1, referring to Fig. 1,2,3, each parts adopt aluminum, stainless steel or other material to make, the base 1 of making is in aggregates by upper and lower right cylinder structure, upper cylinder diameter is less than lower right cylinder, and the two forms boss structure, and 1 centimetre of lower cylinder height, diameter are 3.5 centimetres, its outer wall short transverse has vertical embossing, so that the favourable twisting of increasing friction force; Upper cylindrical body outer wall has external thread, and microslide opening 3 is offered in upper right cylinder bottom, and at microslide opening, microslide 4 is placed at 3 places, for precipitating cerebrospinal fluid, to form inspection specimen, has facilitated operator's operation with microslide 4 simultaneously; The inside of base 1 is concentric recessed base groove 2,2.5 centimetres of base groove 2 diameters, 0.2 centimetre of the degree of depth, the interior installation rubber blanket 5 of base groove 2,2.5 centimetres of rubber blanket 5 diameters, 0.5 centimetre of thickness, effect is that the vibration of buffering microslide is stressed, to prevent its breakage; On microslide 4 in upper cylinder, sample mud chamber 10 is installed, 10 inside, sample mud chamber are tubular structure cell 11, bottom is smooth smooth, easily with microslide close contact and guarantee sealing, tubular structure cell 11 and 10 bottoms, sample mud chamber form a hollow integrated, and the two is boss structure, be convenient to store cerebrospinal fluid, there is opening 12 on 10 tops, sample mud chamber, are convenient to cerebrospinal fluid and add, so that cerebrospinal fluid is deposited on microslide; Mounting nuts 6 outside the upper right cylinder of base 1, nut 6 outer walls have the outside lines of the hand 8 of nut, centre is mud chamber's opening 7, on nut 6 inwalls, process internal whorl 9, on internal whorl 9 and base 1, the external thread of cylindrical body outer wall matches, thereby nut 6 rotates and moves up and down, be easy to fixing microslide; At tubular structure cell 11 overcoat cell lids 13, for preventing that samples of CSF from being polluted.

A kind of cerebrospinal fluid settling vessel described in the utility model, when use first according to illustrated assembly sequency, by the base 1 of the cerebrospinal fluid settling vessel after sterilization, rubber blanket 5, microslide 4, sample mud chamber 10, nut 6 parts such as grade are assembled by follow procedure: rubber blanket 5 is placed in base groove 2, microslide 4 is placed in to the middle suitable position of base 1 by the microslide opening 3 of base, sample mud chamber 10 is put into base 1, and be pressed on microslide 4, nut 6 is passed to sample mud chamber 10, nut 6 inside screws 9 are docked with cylindrical external thread on base 1, by using nut 6, the outside lines of the hand 8 of nut, internal whorl 9 screws nut, the lower plane of the tubular structure cell 11 of the settling vessel making and microslide 4 close contacts are to sealing state, then 1 milliliter of autoprecipitation chamber upper end open 12 of cerebrospinal fluid is joined in mud chamber's tubular structure cell 11, cell lid 13 is held on mud chamber's upper end open 12, to refrigerator cold-storage district, (temperature of chill space is 4 degrees Celsius to manual mobile whole settling vessel, can make the cell death that loses activity as dry under dry or high temperature at room temperature, dead cellular morphology changes, classified counting of leucocyte is brought to certain difficulty), remove cell lid, refrigeration was to about 12 hours natural drying precipitations, whether take out the observation of cerebrospinal fluid settling vessel has precipitated, continuing to be as unfinished placed into refrigerator cold-storage district precipitates again, dismantle and take out microslide 4 as completed according to the inverse process of assembling, microslide 4 is moved to micro-Microscopic observation.After being cleaned, sterilized, cerebrospinal fluid settling vessel assembly after dismounting leaves Accessorial Tools Storage in stand-by.

By reference to the accompanying drawings the utility model is exemplarily described above; obviously the utility model specific implementation is not subject to the restrictions described above; as long as taked the improvement of the various unsubstantialities that method of the present utility model design and technical scheme carry out; or without improving, design of the present invention and technical scheme are directly applied to other occasion, all within protection domain of the present utility model.

Claims (2)

1. a cerebrospinal fluid settling vessel, it comprises base (1), it is characterized in that, described base (1) is in aggregates by upper and lower right cylinder structure, and upper cylinder diameter is less than lower right cylinder, and the two forms boss structure, lower cylindrical outer wall short transverse has vertical embossing, upper cylindrical body outer wall is established external thread, and microslide opening (3) is established in upper right cylinder bottom, locates to establish microslide (4) at microslide opening (3); The inside of base (1) is concentric recessed base groove (2), establishes rubber blanket (5) in base groove (2); Upper this mud chamber of bidding (10) of microslide (4) in upper cylinder, inside, sample mud chamber (10) is tubular structure cell (11), tubular structure cell (11) forms a hollow integrated with bottom, sample mud chamber (10), the two is boss structure, and there is opening (12) on top, sample mud chamber (10); At the upper right cylinder peripheral hardware nut (6) of base (1), nut (6) outer wall is established the outside lines of the hand of nut (8), mud chamber's opening (7) is established in centre, on nut (6) inwall, establishes internal whorl (9), and internal whorl (9) matches with the external thread of the upper cylindrical body outer wall of base (1); At tubular structure cell (11) overcoat cell lid (13).

2. a kind of cerebrospinal fluid settling vessel according to claim 1, is characterized in that, described tubular structure cell (11) bottom is smooth smooth.

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