CN203613195U - Polymerase chain reaction detecting instrument - Google Patents
Polymerase chain reaction detecting instrument Download PDFInfo
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- CN203613195U CN203613195U CN201320824092.6U CN201320824092U CN203613195U CN 203613195 U CN203613195 U CN 203613195U CN 201320824092 U CN201320824092 U CN 201320824092U CN 203613195 U CN203613195 U CN 203613195U
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- polymerase chain
- chain reaction
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 53
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- 238000012360 testing method Methods 0.000 claims description 14
- 238000012216 screening Methods 0.000 claims description 11
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a polymerase chain reaction detecting instrument. The polymerase chain reaction detecting instrument comprises a heating system, a detecting system and a cooling system, wherein the heating system is used for performing non-contact heating on reagents; the detecting system is used for detecting reaction results; the cooling system is used for cooling the reagents; the heating source of the heating system emits heating waves to act on the reagents directly or indirectly; exciting waves are screened by a second light filter, and then collected through a second lens, and collected exciting waves act on the reagents; the excited reagents emit fluorescence; the fluorescence is collected by a third lens, screened by a third light filter, and then transported to a detector; the detector detects the screened fluorescence and analyze according to the experiment result. The polymerase chain reaction detecting instrument is easy to carry, is not required to be operated in a special laboratory during experiment, and can be applied to occasions of on-site fast inspection and quarantine and emergency detection.
Description
Technical field
The utility model relates to polymerase chain reaction device, particularly relates to a kind of polymerase chain reaction detector.
Background technology
In biology field, polymerase chain reaction technique gains universal acceptance, is the effective means with high reliability of a kind of DNA of exploration.DNA in sample, under reagent coordinates, carries out temperature cycle reaction under specified temp program, can obtain at short notice the fragment amplification of 2 Nth power quantity, makes to detect very sensitive.
Fluorescence quantitative polymerase chain reaction test set, as a kind of mainstream technology and the also popularization and application of equipment of biology field, has greatly promoted the development and progress of biology field, for mankind's health and safety contributes.
There is the deficiency of several respects in existing quantitative polyase chain reaction detection system and equipment, has affected the application of the occasions such as quick test quarantine at the scene, Emergent detection:
1) be conventionally with semiconductor refrigeration system and heat-removal system, device structure complexity, volume is large, weight is large.
2) be generally suitable for the detection of large volume, high quality sample.
3) conventionally need complicated layoutprocedure, and carry out in special polymerase chain reaction laboratory.
Utility model content
Based on this, be necessary for the problems referred to above, a kind of portable polymerase chain reaction detector that can realize on-the-spot quick test quarantine, Emergent detection is provided.
A kind of polymerase chain reaction detector, comprise heating system, detection system and cooling system, described detection system comprises stimulating module and detection module, described heating system takes cordless to heat reaction reagent, described heating system comprises heating source, described heating source sends and adds heat wave, the described heat wave that adds acts on reaction reagent, described stimulating module comprises driving source, the second spectral filter and the second lens, described driving source sends field wave, described field wave is through described the second spectral filter screening, described the second lens are collected the field wave after screening and act on reaction reagent so that reaction reagent inspires fluorescence, described detection module comprises the 3rd spectral filter, the 3rd lens and detector, described the 3rd lens are collected described fluorescence and transfer to described detector after described the 3rd spectral filter screenings.
In an embodiment, described polymerase chain reaction detector also comprises the plugged pcr plate that holds reaction reagent therein.
In an embodiment, described heating system is positioned at described pcr plate below therein, and described heating source is ir radiation thermal source.
Therein in an embodiment, described heating system is positioned at described pcr plate top, described heating system also comprises the first spectral filter and first lens, described the first spectral filter screens the described heat wave that adds, described first lens is collected adding heat wave and acting on reaction reagent after screening, described heating source is heated light sources, and described driving source is exciting light source.
In an embodiment, described the first spectral filter is spectral filter therein, and described first lens is focusing optics.
In an embodiment, described the second spectral filter is spectral filter therein, and described the second lens are focusing optics.
In an embodiment, described the 3rd spectral filter is spectral filter therein, and described the 3rd lens are focusing optics, and described detector is fluorescence detector.
In an embodiment, described heating source is microwave heating source or magnetic energy heating source therein, described in add heat wave and described field wave wavelength do not repeat.
In an embodiment, described cooling system comprises blower fan therein.
In an embodiment, described polymerase chain reaction detector also comprises a temperature testing organization therein, and described temperature testing organization detects by infrared detection mode.
Above-mentioned polymerase chain reaction detector, comprises heating system, detection system and cooling system, and heating system heats reaction reagent, and detection system is for detection of reaction result, and cooling system carries out cooling to reaction reagent.The heating source of heating system directly or indirect action on reaction reagent, make reaction reagent start reaction.Field wave is rear by the second lens collection by the second spectral filter screening, field wave after collecting acts on reaction reagent, reaction reagent after being energized ripple and exciting produces fluorescence, described fluorescence is screened by the 3rd spectral filter after being collected by the 3rd lens, fluorescence after detector detection is screened, thus judge according to interpretation.This polymerase chain reaction detector is easy to carry about with one, and need in special laboratory, not operate while testing, so can meet the application of the occasions such as on-the-spot quick test quarantine, Emergent detection.
Accompanying drawing explanation
Fig. 1 is the polymerase chain reaction detector principle schematic of embodiment one;
Fig. 2 is the vertical view of pcr plate;
Fig. 3 is the polymerase chain reaction detector principle schematic of embodiment two.
Embodiment
Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail, thereby so that advantage of the present utility model and feature can be easier to be it will be appreciated by those skilled in the art that, protection domain of the present utility model is made to more explicit defining.
Please refer to Fig. 1 and Fig. 2, it is the first embodiment of the present utility model:
Please refer to Fig. 1, an embodiment of the present utility model provides a kind of polymerase chain reaction detector 100.This polymerase chain reaction detector 100 comprises heating system, detection system and cooling system, and heating system, detection system are all positioned at pcr plate 140 tops, and cooling system is positioned at the below of pcr plate 140.In the time that heating system is positioned at pcr plate 140 top, in heating line, adopting the first spectral filter, first lens to prevent from adding heat wave 114 affects detection system.Heating system takes cordless to heat reaction reagent 142, reaction reagent 142 after heating starts to test reaction, detect testing reacted reaction reagent 142 by detection system, the reference substance of detected result and concentration known is analyzed, thereby reaches a conclusion.Cooling system is cooling for reaction reagent 142 is carried out, and adopts the mode of blower fan blowing in present embodiment, can also adopt in other embodiments the modes such as mounting semiconductor refrigerator to realize, and is not specifically limited herein.The cooperation of heating system and cooling system, the temperature of control reaction reagent 142 that can be stable.Detection system includes stimulating module and detection module, and this stimulating module is as transmitting terminal, and detection module is as the receiving end corresponding with transmitting terminal.
Heating system takes cordless to heat, and the each element in the heating line of heating system adopts non-electric-connecting mode to interact.Heating line in heating system comprises heating source 111, the first spectral filter 112 and first lens 113, and these three devices adopt non-electric-connecting mode, realize contactless heating.The effect of the first spectral filter 112 be filter out required wave band add heat wave 114, the effect of first lens 113 be collect passed through that the first spectral filter 112 screens add heat wave 114, its principle of heating is to adopt the heat wave 114 that adds of specific wavelength to heat reaction reagent 142.What heating source 111 sent add heat wave 114 is collected by first lens 113 after by the first spectral filter 112 screenings, and the heat wave 114 that adds having passed through after first lens 113 is collected acts on reaction reagent 142.
In the present embodiment, heating source 111 is light source, and the first spectral filter 112 is spectral filters, and first lens 113 is focusing optics.The light-wave irradiation that light source sends is on spectral filter, the effect of this spectral filter is to see through for the light wave of the required wave band of experiment, see through the light-wave irradiation of spectral filter on focusing optics, be focused after optics focusing is collected and be radiated on reaction reagent 142 and heat.In other embodiments, heating source can also be microwave heating source, magnetic energy heating source etc., the first spectral filter 112 and first lens 113 are made adaptations according to heating source 111, as long as what the first spectral filter 112 can filter out this wave band adds heat wave 114, first lens 113 can collect see through the first spectral filter 112 add heat wave 114, be not specifically limited herein.The selection principle of heating source 111 is that detector 133 is insensitive to this heating source 111, and this heating source 111 can provocative reaction reagent 142.
Excitation wires on stimulating module comprises driving source 121, the second spectral filter 122 and the second lens 123, the effect of the second spectral filter 122 is that to filter out the effect of the field wave of required wave band 124, the second lens 123 be to collect to have passed through the field wave 124 that the second spectral filter 122 screens.Its excitation principle is to adopt the field wave 124 of specific wavelength to carry out provocative reaction reagent 142, makes the reaction reagent 142 after being excited send fluorescence 134.Stimulating module is for provocative reaction reagent 142, and detection module is for the fluorescence 134 receiving and detection reaction reagent 142 sends.The field wave 124 that driving source 121 sends is collected by the second lens 123 after screening by the second spectral filter 122, and the field wave 124 having passed through after the second lens 123 are collected acts on reaction reagent 142.In the present embodiment, field wave 121 is exciting light source, and the second spectral filter 122 is spectral filter, and the second lens 123 are focusing optics.Exciting light source is radiated on spectral filter, and the effect of this spectral filter is to see through for the excitation light wave of the required wave band of experiment, and the wave band that should see through the excitation light wave of the second spectral filter 122 does not coincide with the wave band of the light wave that sees through the first spectral filter 112.The excitation light wave that sees through spectral filter is radiated on focusing optics, and focusing optics is radiated on reaction reagent 142 after collecting the excitation light wave irradiating thereon, thereby provocative reaction reagent 142 sends fluorescence 134.In other embodiments, driving source 121 can also be microwave driving source, magnetic energy driving source etc., as long as can play the effect of provocative reaction reagent 142.The second spectral filter 122 and the second lens 123 are followed the variation of driving source 121 and corresponding change, as long as can meet the effect of its correspondence, is not specifically limited herein.
Fluorescence circuit on detection module comprises the 3rd lens 131, the 3rd spectral filter 132 and detector 133, the effect of the 3rd lens 131 is that to collect the effect of fluorescence 134, the three spectral filters 132 that reaction reagent 142 sends after being excited be the fluorescence 134 of required wave band in the fluorescence 134 obtaining after collection.In the present embodiment, the 3rd lens 131 are focusing optics, and the 3rd spectral filter 132 is spectral filters, and detector 133 is fluorescence detectors.After being excited, reaction reagent 142 can send fluorescence 134, it is collected and be radiated on spectral filter that this fluorescence 134 is focused optics, the effect of spectral filter is the fluorescence 134 that filters out its all band for the fluorescence 134 that sees through the required wave band of experiment sees through, fluorescence detector detects the fluorescence 134 that sees through spectral filter, the contrast reference substance of concentration known and the polymerase chain reaction information of sample, the quantity that can judge DNA in articles for use or RNA original segments, realizes qualitative or quantification detected result.
Please refer to Fig. 2, this polymerase chain reaction detector 100 also comprises pluggable pcr plate 140.The hole 141 that offers several carrying reaction reagents 142 on this pcr plate 140, is preinstalled with reagent and reaction material in this hole 141.Liquid reagent adopts film mulching method to make conventionally, and solid-state reagent adopts the techniques such as freeze-drying to make conventionally, is then fixed in hole 141 by the mode applying, and user only need add sample and/or water and can complete the preparation of reaction reagent 142, thereby increases work efficiency.On this pcr plate 140, also paste or be printed with bar code or the Quick Response Code 144 of reagent relevant information, special its information of code reader automatic acquisition for the setting of polymerase chain reaction parameter and detected result automatic analysis, diagnosis on polymerase chain reaction detector 100 when use.
On pcr plate 140, be also provided with locating mechanism 143, this locating mechanism 143 is used to indicate the plug direction of this pcr plate 140 on polymerase chain reaction detector 100, can prevent that pcr plate 140 is by dislocation, rotation or upset.
On polymerase chain reaction detector 100, be also provided with temperature testing organization, what this temperature testing organization adopted is infrared detection mode, in other embodiments, can also adopt the contactless modes such as microwave mode, light wave mode or magnetic energy mode to carry out temperature detection.Adopt the optical focus mode of specific wavelength light source to add thermal control to the reaction reagent 142 in hole 141, on cooling system, adopt blower fan to carry out cooling to the mode of drying in module, and detecting the temperature of pcr plate 140 with cordless, three's co-ordination forms stable temperature control.
Please refer to Fig. 3, it is the second embodiment of the present utility model:
The difference of present embodiment and embodiment one is, present embodiment provides a kind of polymerase chain reaction detector 200.Heating system on this polymerase chain reaction detector 200 is positioned at the below of pcr plate 240, directly pcr plate 240 is heated, pcr plate 240 transfers heat to reaction reagent 242, thereby realizes the indirect heating to reaction reagent 242.The heating source 211 of this heating system is ir radiation thermal source, and the sphere of action of this ir radiation thermal source can not arrive pcr plate 240 tops, and therefore ir radiation thermal source can not have influence on the normal work of detection system.Can not arrive the top of pcr plate 240 in order to ensure the sphere of action of ir radiation thermal source, can adopt non-transparent material to make pcr plate 240, utilize the non-transparency of pcr plate 240, make ir radiation ripple 214 that ir radiation thermal source sends not affect the detection system of pcr plate 240 tops.In other embodiments, can also adopt other modes to prevent that ir radiation ripple 214 from affecting the detection system of pcr plate 240 tops, for example take to add the modes such as baffle plate between ir radiation thermal source and pcr plate 240, be not specifically limited herein.
This polymerase chain reaction detector is mainly made up of heating line, excitation wires and fluorescence circuit.The heat wave that adds in heating line adopts cordless to heat reaction reagent, the field wave that excitation wires is mainly chosen specific wavelength adopts type of focusing provocative reaction reagent, and fluorescence circuit be mainly collect, the fluorescence of specific wavelength in fluorescence that detection reaction reagent place sends.On whole polymerase chain reaction detector, can form one or more groups such sense channel, detect application thereby realize polychrome.The power supply of main frame is electric main or battery, and its complete machine can be portable, is applicable to the occasions such as bedside diagnostic instrments, inspection and quarantine, legal medical expert's criminal investigation, battlefield emergency detection and fast qualitative detection by quantitative.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (10)
1. a polymerase chain reaction detector, it is characterized in that, comprise heating system, detection system and cooling system, described detection system comprises stimulating module and detection module, described heating system takes cordless to heat reaction reagent, described heating system comprises heating source, described heating source sends and adds heat wave, the described heat wave that adds acts on reaction reagent, described stimulating module comprises driving source, the second spectral filter and the second lens, described driving source sends field wave, described field wave is through described the second spectral filter screening, described the second lens are collected the field wave after screening and act on reaction reagent so that reaction reagent inspires fluorescence, described detection module comprises the 3rd spectral filter, the 3rd lens and detector, described the 3rd lens are collected described fluorescence and transfer to described detector after described the 3rd spectral filter screenings.
2. polymerase chain reaction detector according to claim 1, is characterized in that, described polymerase chain reaction detector also comprises the plugged pcr plate that holds reaction reagent.
3. polymerase chain reaction detector according to claim 2, is characterized in that, described heating system is positioned at described pcr plate below, and described heating source is ir radiation thermal source.
4. polymerase chain reaction detector according to claim 2, it is characterized in that, described heating system is positioned at described pcr plate top, described heating system also comprises the first spectral filter and first lens, described the first spectral filter screens the described heat wave that adds, described first lens is collected adding heat wave and acting on reaction reagent after screening, and described heating source is heated light sources, and described driving source is exciting light source.
5. polymerase chain reaction detector according to claim 4, is characterized in that, described the first spectral filter is spectral filter, and described first lens is focusing optics.
6. polymerase chain reaction detector according to claim 4, is characterized in that, described the second spectral filter is spectral filter, and described the second lens are focusing optics.
7. polymerase chain reaction detector according to claim 4, is characterized in that, described the 3rd spectral filter is spectral filter, and described the 3rd lens are focusing optics, and described detector is fluorescence detector.
8. polymerase chain reaction detector according to claim 1, is characterized in that, described heating source is microwave heating source or magnetic energy heating source, described in add heat wave and described field wave wavelength do not repeat.
9. polymerase chain reaction detector according to claim 1, is characterized in that, described cooling system comprises blower fan.
10. according to the polymerase chain reaction detector described in any one in claim 1-9, it is characterized in that, described polymerase chain reaction detector also comprises a temperature testing organization, and described temperature testing organization detects by infrared detection mode.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320824092.6U CN203613195U (en) | 2013-12-13 | 2013-12-13 | Polymerase chain reaction detecting instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320824092.6U CN203613195U (en) | 2013-12-13 | 2013-12-13 | Polymerase chain reaction detecting instrument |
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| CN203613195U true CN203613195U (en) | 2014-05-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103645167A (en) * | 2013-12-13 | 2014-03-19 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103645167A (en) * | 2013-12-13 | 2014-03-19 | 苏州东胜兴业科学仪器有限公司 | Polymerase chain reaction detector |
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Granted publication date: 20140528 |