CN203555092U - Liquid nitrogen filtering device for sealing-type cryopreservation of biological materials - Google Patents
Liquid nitrogen filtering device for sealing-type cryopreservation of biological materials Download PDFInfo
- Publication number
- CN203555092U CN203555092U CN201320744675.8U CN201320744675U CN203555092U CN 203555092 U CN203555092 U CN 203555092U CN 201320744675 U CN201320744675 U CN 201320744675U CN 203555092 U CN203555092 U CN 203555092U
- Authority
- CN
- China
- Prior art keywords
- liquid nitrogen
- filter
- pressure vessel
- pipe
- nitrogen pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 216
- 239000007788 liquid Substances 0.000 title claims abstract description 108
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 108
- 239000012620 biological material Substances 0.000 title claims abstract description 20
- 238000001914 filtration Methods 0.000 title abstract description 12
- 238000005138 cryopreservation Methods 0.000 title abstract 5
- 241000700605 Viruses Species 0.000 claims abstract description 6
- 229910052751 metal Inorganic materials 0.000 claims description 9
- 239000002184 metal Substances 0.000 claims description 9
- 150000002739 metals Chemical class 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 10
- 238000007710 freezing Methods 0.000 description 4
- 230000008014 freezing Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000004017 vitrification Methods 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000009172 bursting Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
Abstract
The utility model provides a liquid nitrogen filtering device for sealing-type cryopreservation of biological materials. The liquid nitrogen filtering device comprises a liquid nitrogen pressure vessel, a liquid nitrogen pipe, a filter and an aseptic cylinder, wherein one end of the liquid nitrogen pipe is positioned at the bottom of the liquid nitrogen pressure vessel, and the other end of the liquid nitrogen pipe penetrates through the liquid nitrogen pressure vessel and extends out of the liquid nitrogen pressure vessel to be connected with an inlet of the filter. Filtered liquid nitrogen obtained by the liquid nitrogen filtering device for sealing-type cryopreservation of biological materials can be used for loading the biological materials, so that the biological materials are in an aseptic environment in cryopreservation, and a situation that the biological materials are contaminated by microorganisms such as viruses and bacteria mixed in the liquid nitrogen during cryopreservation is effectively prevented.
Description
Technical field
The utility model relates to a kind of for the sealed frozen device of biomaterial, relates in particular to a kind of for the sealed frozen liquid nitrogen filter of biomaterial.
Background technology
At present, known embryo vitrification freezing carrier has many kinds, and wherein open frozen, embryo directly contacts the frozen of liquid nitrogen, becomes the optimised form of embryo vitrifying freeze because cooling is fast.Its carrier conventionally adopting is that front end has the frozen carrier sheet freezing rod type of (being called for short frozen), and it is simple to operate also efficient fast.
Current embryo vitrification freezing carrier system forms by carrying bar, carry bar cover, carry bar cylinder, carry bar cylinder cap and carrying bar cylinder frame.As scheme as shown in left side, carry bar front end and insert and carry in bar cover, then be loaded into together and carry in bar cylinder, and carry bar cylinder, by clamping, to be carried on bar cylinder frame, its top has is carried the clamping bar cylinder cap that carries of bar cylinder frame and is covered, and prevents from carrying dropping out of bar.Wherein carry on bar frozen for placing embryo, its one end quilt cover of placing embryo is uploaded bar cover, to protect this section not damaged by external force; Carrying bar cylinder carries bar and carries bar cover for loading; Also have an aluminium alloy frame to carry bar cylinder frame, for clamping year bar cylinder and carry bar cylinder cap (carry bar cylinder cap do not drop out cylinder for the protection of article in cylinder outer).Carry on bar and can post biomaterial information labels.Carry bar cylinder frame upper end and can stick the information labels such as frozen position, form a frozen unit.Generally a plurality of samples that derive from same patient are carried to bar and be loaded in same or a plurality of frozen unit, the embryo in different patients source can not load in mixture in same frozen unit, is beneficial to identity marks and resolution
Yet current this open embryo vitrifying freeze bar exists the microorganism that may be sneaked in liquid nitrogen as the risk of the pollutions such as virus and bacteria.The mankind offspring health that this is born by embryo to embryonic germ causes potential threat.
Utility model content
The purpose of this utility model is to overcome above-mentioned deficiency, provide a kind of for the sealed frozen liquid nitrogen filter of biomaterial, prepare aseptic filtration liquid nitrogen, in filtering liquid nitrogen, biomaterial is loaded, in the time of effectively avoiding embryo vitrifying freeze, sneak into the situation of microorganism.
The utility model provides that a kind of described liquid nitrogen filter comprises liquid nitrogen pressure vessel, liquid nitrogen pipe, filter, aseptic tank for the sealed frozen liquid nitrogen filter of biomaterial, wherein,
Described liquid nitrogen pressure vessel is hermetically-sealed construction, is provided with liquid nitrogen and adds mouth and liquid nitrogen is added to the seal cover that mouth seals;
Described liquid nitrogen pipe one end is positioned at the bottom of liquid nitrogen pressure vessel, and the other end runs through the outside that liquid nitrogen pressure vessel extends to liquid nitrogen pressure vessel, is connected with filter inlet;
The outlet of described filter is connected with aseptic tank through connecting drain pipe, or described aseptic tank is positioned at the below of the outlet of filter.
Preferably, in described liquid nitrogen pressure vessel, virus killing uviol lamp is installed.
Preferably, described filter is comprised of metal.
Preferably, described aseptic tank is comprised of metal.
Preferably, described filter is preparation of metals filter screen or preparation of metals shell.
Preferably, the entrance and exit shape size of described filter is identical.
The bend pipe of the hollow that preferably, described liquid nitrogen pipe is both ends open.
Preferably, the position of being run through by liquid nitrogen pipe on described liquid nitrogen pressure vessel is sealing state.
The liquid nitrogen filter that adopts the utility model to provide is prepared aseptic filtration liquid nitrogen and is comprised the following steps:
Liquid nitrogen filter is provided, in liquid nitrogen pressure vessel, add liquid nitrogen, cover tightly seal cover, the liquid nitrogen that the pressure that liquid nitrogen volatilization produces promotes liquid nitrogen pressure vessel bottom automatically flows to filter through liquid nitrogen pipe, after being filtered, flow in aseptic tank, obtain filtering liquid nitrogen;
Preferably, in liquid nitrogen pressure vessel, add after liquid nitrogen, before covering tightly seal cover, can carry out ultraviolet irradiation sterilization to liquid nitrogen.
The utility model is by obtaining filtering liquid nitrogen for the sealed frozen liquid nitrogen filter of biomaterial, in filtering liquid nitrogen, biomaterial is loaded, guaranteed when biomaterial is frozen under gnotobasis, the situation that the microorganism of having been sneaked in liquid nitrogen in the time of effectively having avoided biomaterial frozen pollutes as virus and bacteria etc.
Accompanying drawing explanation
The structural representation for the sealed frozen liquid nitrogen filter of biomaterial that Fig. 1 provides for the utility model.
Embodiment
Below in conjunction with accompanying drawing, by specific embodiment, the utility model is described in detail.
It is a kind of for the sealed frozen liquid nitrogen filter of biomaterial that the present embodiment provides, and described liquid nitrogen filter comprises liquid nitrogen pressure vessel 3, liquid nitrogen pipe 4, filter 5, aseptic tank 6.
Liquid nitrogen pressure vessel 3 is hermetically-sealed construction, is provided with liquid nitrogen interpolation mouth and liquid nitrogen is added to the seal cover 31 that mouth seals, and liquid nitrogen adds mouth and seal cover 31 is threaded connection.
The bend pipe of the hollow that liquid nitrogen pipe 4 is both ends open, one end opening is positioned at the bottom of liquid nitrogen pressure vessel 3, other end opening runs through the outside that liquid nitrogen pressure vessel 3 extends to liquid nitrogen pressure vessel, be connected with the entrance of filter 5, after sealing when the interior interpolation liquid nitrogen of liquid nitrogen pressure vessel 3 and with 31 pairs of liquid nitrogen interpolation mouths of seal cover, the pressure that the liquid nitrogen volatilization in liquid nitrogen pressure vessel 3 produces can automatically promote liquid nitrogen and flow to filter from liquid nitrogen pipe 4.5
The outlet of filter 5 is connected with aseptic tank 6 through connecting drain pipe, or is positioned at the top of aseptic tank 6.
One preferred embodiment in, virus killing uviol lamp is installed in liquid nitrogen pressure vessel 3.
One preferred embodiment in, filter 5 and aseptic tank 6 are comprised of metal, are conducive to prevent bursting by freezing shell, and are conducive to high-temperature sterilization repeatedly and use.
One preferred embodiment in, the entrance and exit shape size of filter 5 is identical, is conducive to two sides and carries out alternate repetition use, rinses while using last time and is blocked in online residue, realizes filter is cleaned.
The liquid nitrogen filter that adopts embodiment to provide is prepared aseptic filtration liquid nitrogen:
Liquid nitrogen filter is provided, in liquid nitrogen pressure vessel, add liquid nitrogen, pass through or sterilize without ultraviolet irradiation, cover tightly seal cover, the liquid nitrogen that the pressure that liquid nitrogen volatilization produces promotes liquid nitrogen pressure vessel bottom automatically flows to filter through liquid nitrogen pipe, after being filtered, flow in aseptic tank, obtain filtering liquid nitrogen.
In liquid nitrogen pressure vessel, add after liquid nitrogen, before covering tightly seal cover, can carry out ultraviolet irradiation sterilization to liquid nitrogen.
Above specific embodiment of the utility model be have been described in detail, but it is just as example, the utility model is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the utility model is carried out and alternative also all among category of the present utility model.Therefore,, not departing from the equalization conversion of doing under spirit and scope of the present utility model and revising, all should be encompassed in scope of the present utility model.
Claims (8)
1. for the sealed frozen liquid nitrogen filter of biomaterial, it is characterized in that, described liquid nitrogen filter comprises liquid nitrogen pressure vessel, liquid nitrogen pipe, filter, aseptic tank, wherein,
Described liquid nitrogen pressure vessel is hermetically-sealed construction, is provided with liquid nitrogen and adds mouth and liquid nitrogen is added to the seal cover that mouth seals;
Described liquid nitrogen pipe one end is positioned at the bottom of liquid nitrogen pressure vessel, and the other end runs through the outside that liquid nitrogen pressure vessel extends to liquid nitrogen pressure vessel, is connected with filter inlet;
The outlet of described filter is connected with aseptic tank through connecting drain pipe, or described aseptic tank is positioned at the below of the outlet of filter.
2. liquid nitrogen filter according to claim 1, is characterized in that, in described liquid nitrogen pressure vessel, virus killing uviol lamp is installed.
3. liquid nitrogen filter according to claim 1, is characterized in that, described filter is comprised of metal.
4. liquid nitrogen filter according to claim 3, is characterized in that, described filter is preparation of metals filter screen or preparation of metals shell.
5. according to the liquid nitrogen filter described in claim 3 or 4, it is characterized in that, the entrance and exit shape size of described filter is identical.
6. liquid nitrogen filter according to claim 1, is characterized in that, described aseptic tank is comprised of metal.
7. liquid nitrogen filter according to claim 1, is characterized in that, the bend pipe of the hollow that described liquid nitrogen pipe is both ends open.
8. liquid nitrogen filter according to claim 1, is characterized in that, the position of being run through by liquid nitrogen pipe on described liquid nitrogen pressure vessel is sealing state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320744675.8U CN203555092U (en) | 2013-11-21 | 2013-11-21 | Liquid nitrogen filtering device for sealing-type cryopreservation of biological materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320744675.8U CN203555092U (en) | 2013-11-21 | 2013-11-21 | Liquid nitrogen filtering device for sealing-type cryopreservation of biological materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203555092U true CN203555092U (en) | 2014-04-23 |
Family
ID=50504857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320744675.8U Expired - Lifetime CN203555092U (en) | 2013-11-21 | 2013-11-21 | Liquid nitrogen filtering device for sealing-type cryopreservation of biological materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203555092U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105413303A (en) * | 2015-11-23 | 2016-03-23 | 王丽 | Liquid nitrogen manometry purifying and filtering device |
-
2013
- 2013-11-21 CN CN201320744675.8U patent/CN203555092U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105413303A (en) * | 2015-11-23 | 2016-03-23 | 王丽 | Liquid nitrogen manometry purifying and filtering device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: ZHEJIANG XINGBO BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SHANGHAI NINTH PEOPLE'S HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIVERSITY SCH Effective date: 20150417 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 200011 HUANGPU, SHANGHAI TO: 315040 NINGBO, ZHEJIANG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150417 Address after: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee after: ZHEJIANG CELLPRO BIOTECH Co.,Ltd. Address before: 200011 Shanghai City, Huangpu District manufacturing Bureau Road No. 639 Patentee before: SHANGHAI NINTH PEOPLE'S HOSPITAL SHANGHAI JIAOTONG University SCHOOL OF MEDICINE |
|
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee after: ZHEJIANG CELLPRO BIOTECH CO.,LTD. Address before: 315040 Zhejiang high tech District of Ningbo City Xinhui Road No. 139 Building No. 2 Patentee before: ZHEJIANG CELLPRO BIOTECH Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140423 |