CN203546019U - Cell enrichment identifying device - Google Patents
Cell enrichment identifying device Download PDFInfo
- Publication number
- CN203546019U CN203546019U CN201320677378.6U CN201320677378U CN203546019U CN 203546019 U CN203546019 U CN 203546019U CN 201320677378 U CN201320677378 U CN 201320677378U CN 203546019 U CN203546019 U CN 203546019U
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- China
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- lid
- base
- microporous membrane
- enrichment
- joint pin
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model belongs to the technical field of cell separation, and in particular relates to a cell enrichment identifying device. The cell enrichment identifying device comprises two parts: a cover body and a base, wherein ports are respectively arranged at the top of the cover body and at the bottom of the base; a hollow connecting pillar is arranged at the top of the base; an outer screw thread is arranged on the periphery of the connecting pillar; an inner screw thread matched with the outer screw thread is formed on the inner wall of the cover body; a microporous membrane is arranged at the top of the connecting pillar; the microporous membrane is pressed and fixed when the cover body is in close threaded connection with the connecting pillar. The cell enrichment identifying device disclosed by the utility model can realize enrichment of pathogenic organisms and pathogenic cells under a pollution-free occasion, so as to directly identify the pathogenic organisms and pathogenic cells.
Description
technical field
The utility model belongs to cell separation technology field, is specifically related to a kind of cell enrichment identification apparatus.
background technology
In technical field of biological, usually need by some cell separation and enrichment in sample out, then identify, common are microorganism enrichment evaluation, fungi enrichment evaluation, parasite enrichment evaluation, virus infected cell enrichment evaluation, pathological cells enrichment evaluation.
Microorganism enrichment is identified: for example for the enrichment of serious infectious diseases tubercule bacillus, identifies, and the enrichment evaluation of enteric pathogenic bacteria, food poisoning bacteria is as the enrichment evaluation of Salmonellas.Fungi enrichment is identified: as the enrichment of aspergillus tubigensis etc. is identified.Parasitic enrichment is identified: as the enrichment of ameba, parasitic ovum (schistosome ovum etc.) etc. is identified.Virus infected cell enrichment is identified: the enrichment that comprises the cells infecteds such as influenza virus, syncytial virus as Respirovirus is identified.Pathological cells enrichment is identified: the enrichment that comprises Exfoliated tumor cells (cancer cells that oral cavity, lung, splanchnocoel, uterine cavity, urine, gastric juice, ight soil etc. come off or gathers) and Peripheral Circulation tumour cell is identified.
In order to guarantee the accuracy of check, often need under the condition of aseptic, sealing, carry out, so also can anti-ly infect, anti-pollution, ensure the safety of operator and surrounding environment, this just needs a kind of tripping device of sealing to realize such check.
summary of the invention
The cell enrichment identification apparatus that provides a kind of closure good is provided the purpose of this utility model.
The utility model is by the following technical solutions:
A kind of cell enrichment identification apparatus, comprise lid and base two portions, the top of lid and the bottom of base are respectively equipped with interface, the top of base is provided with the joint pin of hollow, the periphery of joint pin is provided with outside screw, the inwall of lid is provided with the internal thread suitable with outside screw, and the top of joint pin is provided with microporous membrane, when lid is threaded with joint pin when tight, microporous membrane is fixed.
The external diameter of joint pin is less than the external diameter of base, and the external diameter of lid is identical with the external diameter of base.
The interface at described lid top is connected with specimen collection container, specimen collection container comprises cup, and cup is provided with upper cover and going to the bottom, upper cover and go to the bottom on be respectively equipped with feed port and discharge hole, the position of feed port and discharge hole is equipped with microporous membrane, and discharge hole is connected with the interface at lid top.
The aperture of upper cover feed port place microporous membrane is 0.2 ~ 0.4 micron, prevents that bacterium from running out of; The go to the bottom aperture of discharge hole place microporous membrane is 0.2 ~ 50 micron, impurity trapped and interfering substance; The aperture of joint pin top microporous membrane is 0.2 ~ 30 micron, enrichment causal organism and pathological cells.
The aperture of the microporous membrane at feed port place, discharge hole place, joint pin top can be selected according to the difference of sample and target enriched product.
Microporous membrane is polycarbonate membrane, Parylene film or nitrocellulose filter.
The utility model is provided with the joint pin of hollow on base, is connected, and the microporous membrane at joint pin top is fixed between joint pin and lid by thread seal; Interface on lid is connected with the discharge hole of specimen collection container.
While making a collection of specimens, open the upper cover of specimen collection container, after adding sample, cover immediately upper cover, then make the interface of base be connected with suction unit, open suction unit, sample is downwards by the microporous membrane at discharge hole place, the microporous membrane at discharge hole place is held back impurity and interfering substance, sample continues to enter in lid by the interface at lid top, through the microporous membrane at joint pin top, by causal organism and pathological cells enrichment, the microporous membrane at joint pin top is taken out and is identified.
The utility model has following advantage:
1) can realize pollution-free, without collecting contagiously, transportation and sealing treatment censorship sample: infectivity sample, the tumour patient samples such as bacterium (particularly tubercule bacillus), fungi, virus are not got rid of causal organism existence, the possibility that has infection or pollute, apply this device and can prevent from infecting or polluting surrounding environment, and can be directly in the container of sealing deactivation causal organism, liquefaction mucus etc., this safety to protection operator is very important, and public safety is also had to important value;
2) high power enrichment method causal organism and pathological cells: the microorganism that will detect in the sample gathering or pathological cells and few, several days even tens of skies of some needs cultivate one's ability and reach energy amount detection, by the utility model based on film collection bacterium and collection cell concentration, can several times or hundred times concentrated, reaching can detection level, causal organism and pathological cells can be detected, reach the object of check at once;
3) can be on the microporous membrane of discharge hole fixing mark thing, realize the directly evaluation to checking matter, also can take out microporous membrane and identify separately;
4) can in specimen collection container, add substratum if desired, cultivate causal organism and pathological cells, carry out micro-fast culture, then do other experiments and research.
accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the explosive view up and down of lid, base.
embodiment
A kind of cell enrichment identification apparatus, as shown in Figure 1, 2, comprise lid 1 and base 2 two portions, the bottom of the top of lid 1 and base 2 is respectively equipped with interface 11 and interface 21, the top of base 2 is provided with the joint pin 22 of hollow, and the periphery of joint pin 22 is provided with outside screw, and the inwall of lid 1 is provided with the internal thread suitable with outside screw, the top of joint pin 22 is provided with microporous membrane 23, when lid 1 is threaded with joint pin 22 when tight, microporous membrane 23 is fixed.The external diameter of joint pin 22 is slightly less than the external diameter of base 2, and the external diameter of lid 1 is identical with the external diameter of base 2.
The interface 11 at lid 1 top is connected with specimen collection container, specimen collection container comprises cup 3, cup 3 is provided with upper cover 31 and goes to the bottom 32, upper cover 31 and go to the bottom on 32 and be respectively equipped with feed port 33 and discharge hole 36, the position of feed port 33 and discharge hole 36 is respectively equipped with microporous membrane 34 and microporous membrane 35, and discharge hole 36 is connected with the interface 11 at lid 1 top.
During use, during use, the upper cover 31 of opening specimen collection container joins in cup 3 by sample, after adding, sample covers immediately upper cover 31, the interface 21 of base 2 is connected with suction unit, open suction unit, sample is downwards by the microporous membrane 35 at discharge hole 36 places, microporous membrane 35 is held back impurity and interfering substance, sample continues to enter in lid 1 by the interface 11 at lid 1 top, through the microporous membrane 23 at joint pin 22 tops, by causal organism and pathological cells enrichment, the microporous membrane at joint pin 22 tops 23 is taken out and identified.
Claims (3)
1. a cell enrichment identification apparatus, it is characterized in that, comprise lid and base two portions, the top of lid and the bottom of base are respectively equipped with interface, the top of base is provided with the joint pin of hollow, and the periphery of joint pin is provided with outside screw, and the inwall of lid is provided with the internal thread suitable with outside screw, the top of joint pin is provided with microporous membrane, when lid is threaded with joint pin when tight, microporous membrane is fixed.
2. cell enrichment identification apparatus as claimed in claim 1, is characterized in that, the external diameter of joint pin is less than the external diameter of base, and the external diameter of lid is identical with the external diameter of base.
3. cell enrichment identification apparatus as claimed in claim 1, it is characterized in that, the interface at described lid top is connected with specimen collection container, specimen collection container comprises cup, cup is provided with upper cover and goes to the bottom, upper cover and go to the bottom on be respectively equipped with feed port and discharge hole, the position of feed port and discharge hole is equipped with microporous membrane, discharge hole is connected with the interface at lid top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320677378.6U CN203546019U (en) | 2013-10-31 | 2013-10-31 | Cell enrichment identifying device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320677378.6U CN203546019U (en) | 2013-10-31 | 2013-10-31 | Cell enrichment identifying device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203546019U true CN203546019U (en) | 2014-04-16 |
Family
ID=50464164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320677378.6U Expired - Fee Related CN203546019U (en) | 2013-10-31 | 2013-10-31 | Cell enrichment identifying device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203546019U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552151A (en) * | 2020-04-23 | 2021-10-26 | 北京安科慧生科技有限公司 | X-ray fluorescence spectrometer and ion enrichment device thereof |
-
2013
- 2013-10-31 CN CN201320677378.6U patent/CN203546019U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552151A (en) * | 2020-04-23 | 2021-10-26 | 北京安科慧生科技有限公司 | X-ray fluorescence spectrometer and ion enrichment device thereof |
| CN113552151B (en) * | 2020-04-23 | 2024-04-19 | 北京安科慧生科技有限公司 | X-ray fluorescence spectrometer and ion enrichment device thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 Termination date: 20191031 |