CN203479771U - Device for connecting chromatographic column to pipeline - Google Patents
Device for connecting chromatographic column to pipeline Download PDFInfo
- Publication number
- CN203479771U CN203479771U CN201190000861.XU CN201190000861U CN203479771U CN 203479771 U CN203479771 U CN 203479771U CN 201190000861 U CN201190000861 U CN 201190000861U CN 203479771 U CN203479771 U CN 203479771U
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- Prior art keywords
- spring
- body member
- pipeline
- chamber
- power
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
- B23P—METAL-WORKING NOT OTHERWISE PROVIDED FOR; COMBINED OPERATIONS; UNIVERSAL MACHINE TOOLS
- B23P19/00—Machines for simply fitting together or separating metal parts or objects, or metal and non-metal parts, whether or not involving some deformation; Tools or devices therefor so far as not provided for in other classes
- B23P19/04—Machines for simply fitting together or separating metal parts or objects, or metal and non-metal parts, whether or not involving some deformation; Tools or devices therefor so far as not provided for in other classes for assembling or disassembling parts
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16L—PIPES; JOINTS OR FITTINGS FOR PIPES; SUPPORTS FOR PIPES, CABLES OR PROTECTIVE TUBING; MEANS FOR THERMAL INSULATION IN GENERAL
- F16L37/00—Couplings of the quick-acting type
- F16L37/08—Couplings of the quick-acting type in which the connection between abutting or axially overlapping ends is maintained by locking members
- F16L37/12—Couplings of the quick-acting type in which the connection between abutting or axially overlapping ends is maintained by locking members using hooks, pawls or other movable or insertable locking members
- F16L37/20—Joints tightened by toggle-action levers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6034—Construction of the column joining multiple columns
- G01N30/6039—Construction of the column joining multiple columns in series
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N2030/6008—Construction of the column end pieces capillary restrictors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49826—Assembling or joining
- Y10T29/49904—Assembling a subassembly, then assembling with a second subassembly
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mechanical Engineering (AREA)
- Clinical Laboratory Science (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a device for connecting a chromatographic column to a pipeline. The chromatographic column is a liquid chromatographic column comprising an end joint. The device comprises at least one body member, a first spring, a second spring and a pushing and latching mechanism, wherein the body member comprises at least one chamber; the first spring is arranged in the chamber, and is configured to apply a first force to the pipeline towards the end joint of the column; the second spring is arranged in the at least one chamber, and is configured to apply a second force to a deformable sealing member towards the end joint of the column; the pushing and latching mechanism is configured to push the body member and the first and second springs towards the end joint of the column.
Description
Technical field
Present invention relates in general to liquid phase chromatography, and relate to or rather a kind of for a chromatographic column being fluidly attached to mechanism and the method for delivery fluid line in a liquid chromatographic system.
background technology
Liquid phase chromatography (LC) is well-known in Chemical Decomposition, compound purifying and chemical analysis field.A core component of liquid chromatographic system is chromatographic column.This post comprises a kapillary that is filled with a kind of permeable solid material, and this solid material itself is a kind of chromatographic stationary phases or otherwise comprises or support a kind of chromatographic stationary phases.Make to comprise the fluid mixture that moves phase together with a kind of chromatogram for purifying or separated interested a kind of compound leads an endpiece from an inlet end and flows through this post under pressure.Generally speaking, this fixing mutually and this chemical characteristic that moves phase be make interested compound this move from this fixing phase between assigned degree and the assigned degree of other compounds in this fluid different.Therefore, the reserving degree of compound or reserving degree or the retention time of other compounds of retention time and these interested in this post are different, therefore cause the physical separation of interested compound and these other compounds.
Although chromatographic column can be recycled and reused for, repeatedly analyze or purification process, arbitrary concrete post finally need to be removed and change with a different post from LC system.For example, due to long use, the accumulation of the physics of a kind of column material or fixedly phase or chemical degradation or chemical contamination or particle may cause a post unavailable.As another example, the variation of analysis that may be corresponding from a kind of different interested Compound Phase or separated type may require to change existing post with a different post, and the fixedly phase chemistry character of the post that these are different is better optimized for this new demand.Especially in high power capacity or high flux laboratory environment, may be necessary to connect continually and disconnect various different chromatographic columns.In order a post to be connected to a LC system or a post to be disconnected from a LC system, be necessary to carry out or interrupt flow sports association connects between this post and the two ends of delivery fluid line.The consideration of efficiency is pointed out this connection of a plurality of posts and is disconnected and carrying out simple as much as possible and fast and in the minimum system break situation that should be able to cause in the cut-out by leak fluid or parts.
At present, in order with a kind of usual manner, a post to be attached to a LC system, user must be first correctly be assembled into a pressurizing adaptor and hoop on the end of pipeline of length of a delivery fluid.Importantly this pipeline cut before assembling with meeting at right angles neatly He.This assembly must be inserted in a styletable joint, guarantee that this assembly (pipe, joint and hoop) can not break simultaneously.Then, when this pipeline being pressed onto in this styletable joint, user must use the spanner of two suitable sizes that this pressurizing adaptor is fastened in this styletable joint.User must carefully not make the fastening deficiency of forcing screw, so that this assembly will unclamp and can not seal.User also must carefully not make this forcing screw fastening excessively, because do like this risk that presents this component wear, this forcing screw fracture or this pipeline avalanche.
After this, once this first assembly has correctly been mounted in this styletable joint, from this LC system, removes this post requirement user and reuse two spanners and unclamp and disconnect this pipeline.Although can repeat this program repeatedly with a single component, but finally by abrasion and the fatigue of materials that repeatedly manual operation causes, required to change this assembly (this post, pipeline or joint), to alleviate the risk of wearing and tearing, fracture or the pipeline avalanche of increase subsequently.
Use when front pillar attachment method, this hoop is for being retained on this pipeline this styletable joint and producing a kind of rigid seal (positive seal).Therefore must apply enough power and bind round to this, to guarantee, coordinate by this pipeline fixing in position with the friction/distortion of this pipeline.Typically, this power, can reusable quantity or number of times thereby shortened significantly a concrete assembly much larger than the necessary power of a kind of rigid seal of guaranteeing between this pipe, hoop and styletable joint.
The disclosure of invention
A kind of method of installing and using it is taught in this disclosure, the chromatographic column that these apparatus and method are conducive to a LC is in one way connected and disconnection with the quick of carrying object pipeline, and which can negatively not affect chromatography performance and guarantee the pressure lower seal of those pressure that resulting connection can be found in surpassing this LC system.First this device can be positioned at a pipe in styletable joint so that this pipe will contact with this styletable joint.Once contact, then this device applies a spring force (the first power) to this pipe, and this spring force surpasses when this post is under maximum operating pressure the reacting force producing.Preferably, this first power is should be just in time enough large, with under the on-stream pressure a regulation by this pipeline fixing in position.Then, this device guarantees that a deformable containment member (it can be a hoop) contacts with this same styletable joint, thereby around this pipe.An independent independently spring force (the second power) is applied to this deformable containment member or hoop, to guarantee to carry out a kind of suitable Fluid Sealing between this pipeline, sealing member or hoop and this styletable joint, to prevent any seepage under the maximum operating pressure of this post.Preferably, this second power should be just in time enough large, to produce this suitable Fluid Sealing under the pressure of regulation.By a promotion and locking (or locking) mechanism, provide the location of this pipe, containment member or hoop and styletable joint and for these spring forces of this pipe and containment member or hoop, the handle that this mechanism for example comprises in this device, this handle provides a kind of motion of appropriate amount and mechanical advantage so that operator does not need an instrument.
In the first aspect of originally teaching content, disclose that a kind of this chromatographic column comprises an end connector for a liquid-phase chromatographic column being attached to the device of a pipeline, this device comprises: at least one body member, it comprises at least one chamber; First spring in this at least one chamber, this first spring is configured to apply first power to the pipeline of this length towards this styletable joint; Second spring in this second chamber, this second spring is configured to apply second power to an one deformable containment member towards this styletable joint; A supporting member movably, it is fixed to this at least one body member; And promotion and locking or locking mechanism, it is configured to promote this at least one body member, the first and second springs and supporting member movably towards this styletable joint.In some embodiments, this device also comprises a movably supporting member, this supporting member is fixed to this at least one body member, wherein this promotion and blocking mechanism are further configured to towards this styletable joint and promote this movably supporting member, and this at least one body member comprises: a near-end body member, and this near-end body member is fixed to this movably supporting member and have and be configured to the first end and second end that coordinate with this styletable joint; An intermediate body member member, this intermediate body member member comprises the intermediate body member member first end and intermediate body member member second end that are attached to this second end of this near-end body member; An and far-end body member, this far-end body member is attached to this intermediate body member member the second end, wherein this at least one chamber comprises first chamber and second chamber, this first chamber comprises this first spring and by this intermediate body member member and this far-end body member gauge, and this second chamber comprises this second spring and by this intermediate body member member and this near-end body member gauge.Sealing member can comprise a hoop, and this device may further include one second hoop, and this second hoop is in a hollow interior part of this intermediate body member member; And first lining ring or packing ring, this first lining ring or packing ring are placed between this first spring and this second hoop, and wherein this second hoop and this first lining ring or packing ring are configured to make this first spring to apply this first power to the pipeline of this length with this second hoop by this first lining ring or packing ring.This second hoop is to be formed by a kind of material, and this material has the hardness identical with a kind of wall material of the pipeline of this length or harder than a kind of wall material of the pipeline of this length.
In other embodiments, at least one body member comprises a piston and further comprises a housing, and this housing comprises: a supporting construction, for supporting this end connector; And hole or ，Gai hole, chamber or chamber have a part, by this promotion and blocking mechanism, promoting in this Piston Process, a part for this piston moves slidably in this part.In these other embodiments, device, further comprises the collar with a flange, and this flange matches for power is transferred to this pipeline from this first spring with a groove in this pipeline.
In the second aspect of originally teaching content, disclose that a kind of this chromatographic column comprises an end connector for a liquid-phase chromatographic column being attached to the device of a pipeline, this device comprises: a piston, it comprises at least one chamber; First spring in this at least one chamber, this first spring is configured to apply first power to the pipeline of this length towards this styletable joint; Second spring in this at least one chamber, this second spring is configured to apply second power to an one deformable containment member towards this styletable joint; One promotes and blocking mechanism, and it is configured to promote this piston, this first spring and this second spring towards this styletable joint; And a housing, it comprises for this end connector and has the supporting construction in a hole or chamber, by this promotion and blocking mechanism, promoting in the process of this piston, a part for this piston moves slidably in this hole or chamber.
In the third aspect of originally teaching content, disclose a kind of for a liquid-phase chromatographic column being attached to the device of first pipeline and second pipeline, this chromatographic column comprises a first end with a first end joint and second end with second end connector, this device comprises: pedestal and be fixed to first coupling arrangement and second coupling arrangement of this pedestal, each coupling arrangement is used for one of this first and second end connector one of being attached in this first and second pipeline, each coupling arrangement comprises at least one body member, this at least one body member comprises (a) at least one chamber, (b) first spring in this at least one chamber, this first spring is configured to apply first power to the pipeline of this length towards this styletable joint, (c) second spring in this at least one chamber, this second spring is configured to apply second power to an one deformable containment member towards this styletable joint, and (d) one promote and blocking mechanism, it is configured to promote this at least one body member towards this post, this first spring and this second spring.In some embodiments, described device, wherein at least one in this first and second coupling arrangement further comprises: a supporting member movably, this supporting member is fixed to this at least one body member, and wherein this promotion and blocking mechanism are further configured to towards coupled end connector and promote this movably supporting member; A near-end body member, this near-end body member is fixed to this movably supporting member and have and be configured to the first end and second end that match with coupled end connector; An intermediate body member member, this intermediate body member member comprises the intermediate body member member first end and intermediate body member member second end that are attached to this second end of this near-end body member; An and far-end body member, this far-end body member is attached to this intermediate body member member the second end, wherein this at least one chamber comprises first chamber and second chamber, this first chamber comprises this first spring and by this intermediate body member member and this far-end body member gauge, and this second chamber comprises this second spring and by this intermediate body member member and this near-end body member gauge.In other embodiments, described device, wherein at least one in this first and second coupling arrangement further comprises: a housing, this housing comprises: a supporting construction, for supporting coupled end connector; And hole or chamber, this hole or chamber have a part, by this promotion and blocking mechanism, promoting in the process of this at least one body member, a part for this at least one body member moves slidably in this part, and wherein this at least one body member comprises a piston.These other true mode can further include the collar with a flange, and this flange matches for power is transferred to coupled pipeline from this first spring with a groove in coupled pipeline.
In the fourth aspect of originally teaching content, disclose a kind of for a pipeline being attached to the method for a liquid-phase chromatographic column, this pipeline has an end of line, the method comprises the following steps: (a) part for this pipeline (comprising this end of line) is passed to a coupling arrangement, this device comprises: (i) at least one chamber; (ii) first spring, it in this at least one chamber, a spring force can be transferred to this pipeline; (iii) second spring in this at least one chamber; And (iv) deformable containment member, it can receive second power from this second spring; (b) this end of line is inserted in the hollow container of an end connector of this liquid-phase chromatographic column; (c) towards this chromatographic column, move this coupling arrangement so that this first spring drives this pipeline to enter in this container, so that this end of line surpasses a maximum operating fluid pressure of this chromatographic column for a pressure of this end connector; And (d) further towards this chromatographic column, move this coupling arrangement and between this post and this container, form a kind of Fluid Sealing so that this second spring causes this deformable containment member.
Brief Description Of Drawings
From following, only by way of example and with reference to the given explanation of the accompanying drawing that needn't draw in proportion, above-described and various other aspects of the present invention will become clear, in the accompanying drawings:
Fig. 1 is the schematic illustrations of conventional liquid chromatography-mass spectrography (LCMS) system of a broad sense;
Fig. 2 is the schematic illustrations with a liquid-phase chromatographic column of conventional connector, and these connectors are to be connected with an intake pipeline and an export pipeline;
Fig. 3 A be according to originally teach content for a pipeline being attached to a kind of diagram of device of one end of a chromatographic column;
Fig. 3 B is the skeleton view of the post fixed mechanism part of Fig. 3 A;
Fig. 4 A be according to originally teach content for a pipeline being attached to a kind of diagram of a part of the second device of one end of a chromatographic column;
Fig. 4 B be according to originally teach content for a pipeline being attached to a kind of diagram of a part of the 3rd device of one end of a chromatographic column;
Fig. 5 be according to originally teach content for an intake pipeline and export pipeline being attached to a kind of diagram of system of the corresponding end of a chromatographic column;
Fig. 6 A be according to originally teach content for a pipeline is attached to a chromatographic column one end a kind of the 4th device the first skeleton view;
Fig. 6 B be in Fig. 6 A the second skeleton view of graphic device;
Fig. 6 C be by Fig. 6 A, 6B graphic device center and along the obtained viewgraph of cross-section of the main axis of this device;
Fig. 6 D is can be for the skeleton view of a fluid line in the device of Fig. 6 A, 6B; And
Fig. 7 be according to originally teach content for pipe string being attached to the process flow diagram of a kind of method of a chromatographic column.
Implement pattern of the present invention
Below the proposition of explanation is in order to make any technician of this area can carry out and use the present invention, and is to provide under the background of specific application and requirement thereof.To those skilled in the art, to the various modifications of described embodiment, will be apparent, and can be applied to other embodiment in this rule.Therefore, the present invention is not intended to be limited to shown embodiment and example, but will give possible wide region according to shown and described feature and principle.In order to understand in more detail fully feature of the present invention, incorporated by reference to discussing referring to Fig. 1 to Fig. 6 below.
Fig. 1 is the schematic illustrations of a conventional liquid chromatography-mass spectrography (LCMS) system.System 10 shown in Fig. 1 comprise for a kind of aqueous chemical potpourri is separated into it component substances chromatographic column 7 be fluidly attached to post 7 for detect or differentiate their mass spectrometer 30 when these separated component substances receive in order from post 7.Although illustrate a mass spectrometer for exemplary purpose, depend on user's demand, in order to detect or differentiate the object of the composition that these are separated, mass spectrometer part 30 can be replaced with an instead of chemical analytical equipment.For example, can adopt for this purpose an Infrared Transmission or fluorescence detector.Other pick-up units are known.
Still referring to Fig. 1, an input end can observing post 7 is be fluidly attached to an output port of mixing arrangement 5 and received the sample of this output port and the potpourri of solvent fluid from this mixing arrangement by fluid line 6d.These different chemical compositions of this potpourri move from one that fills in this post fixing these heterogeneities that have difference to distribute to cause between mutually and have difference to retain and different corresponding time of the wash-out of these compositions from this post output terminal to fluid line 6e subsequently in this post at this.An optional diverting valve 12 can be separated into a splitter section by these eluted materials along fluid line 6f, and it is delivered to refuse or reservoir vessel 14, and an analysis part, and it is delivered to mass spectrometer 30.This mass spectrometer can comprise the various parts of knowing, and as an atmospheric pressure ionization source 15, it is delivered to a stream with the charged particle 16 of the composition that comprises ionization in an ionization chamber 18.Resulting charged particle is to receive to one or more vacuum chambers 19 by an aperture 20, at least one in this or these vacuum chamber comprise for according to corresponding matter/lotus score of these ions from their mass-synchrometer 21 and one or more detecting devices 22 for detection of these separated ions.
Fig. 2 is the schematic illustrations of a conventional liquid-phase chromatographic column and its fitting that is associated that is attached to fluid line.Post 7 receives a kind of fluid mixture at an input end from fluid line 6d, and this potpourri is separated into its composition and in corresponding elution time, these separated compositions is outputed to fluid line 6e from an output terminal.This post comprises a hollow kapillary 25, and this inside capillaceous is filled with a kind of porosint 27, and as a kind of particulate material of classification by size, this material comprises this fixedly phase.End cap 29 on arbitrary end of kapillary 25 is for being retained in this porosint in kapillary 25 and keeping the on-stream pressure in this pipe.This post one end in office is equipped with end connector 31, and these end connectors are formed for the part for a pressurizing adaptor of a fluid line of end cap seal (6d, 6e) separately.In some enforcements or design, this styletable lid may not comprise independent parts, but can be integral with this end connector on the contrary, or this end connector can provide the function of an end cap.In this document, use in a broad sense term " end connector " to comprise independent end cap, there is the joint of end cap of one or independent end connector and the end cap that is bonded to each other and uses in pairs.Each end at post completes pressurization by a hoop 32, this hoop can be by a threaded nut or the tight extrusion of screw 33 to a hole of the joint 31 being associated, to be out of shape to produce a kind of mode of leakproof seal between this hoop, fluid line and end connector.
Fig. 3 A be according to originally teach content for a pipeline being attached to the diagram of a kind of device 100 of one end of a chromatographic column.In operation, the axis that pipeline 6 is parallel to this device haply passes completely through device 100 by the different holes 111 of this device.Device 100 is exercisable to apply a power to pipeline 6, so that this pipeline is depressed in end connector 104, to form fluid with post 103, connects.Device 100 is that further exercisable to apply second power to an one containment member 122b(, it can be a hoop), to sealing member is out of shape to produce a kind of mode of leakproof seal between pipeline 6, end connector 104 and post 103.The coaxial line that described power is both parallel to device 100 and post 103 haply applies, thereby prevents that any twisting motion or power are applied to this post.
Supporting member 116(Fig. 3 A of device 100) engage with pedestal or housing 108 to be only movably haply in one direction with respect to this housing.This slidably joint can be for example by implementing with a guide rail (not shown).This guide rail can be to be attached to rigidly this supporting member and to be designed to slide in a coupling groove (not shown) in this pedestal or housing.The those of ordinary skill of mechanical field can easily design other slidably engagement arrangement and connections.
Compare with the slidably character connecting between pedestal or housing 108 with supporting member 116, post supporting member 106 is to be rigidly fixed to appropriate location with respect to pedestal or housing 108.As shown in Figure 3 B, post supporting member 106 comprises part 107 protrusion or recessed, and this part is designed to a part that matches and partly surround this styletable joint with a part for styletable joint 104.This styletable joint or this column holder can be to be become by a kind of slightly pliable and tough material structure, so that when this post moves up in the side of the downward directional arrow of Fig. 3 B under power, in that define and the reproducible position of end connector 104 " card " in protuberance 107 one together with post 103.In the operating process a pipeline 6 being attached to this post, by installing 100 while applying force to the free end of this post, the shut-down mechanism of styletable joint 104 (as circumferential ridge 105) prevents the movement of this end connector and post.Although this shut-down mechanism is illustrated as a circumferential ridge 105 in Fig. 3 B, it can alternately be embodied as a kind of multi-form outstanding, as boss, protuberance or a pin.Between supporting member 116 and pedestal or housing 108 slidably connect and post supporting member 106 and this pedestal or housing between the combination being fixedly connected allow device 100(to comprise assembly and the associated components discussed further below with three body members 110,112,114) by be parallel to device 100 an axis mobile orientation column 103 and its relevant column joint 104 moves or retract from this post and its relevant column joint.Optionally, this slidably connects can be equipped with a locking or locking mechanism to prevent mobile when reaching a desirable position.
Return to now the discussion of Fig. 3 A, can easily observe device 100 and further comprise that two springs that are assembled in this device are to provide the independent spring force of an axis that is parallel to this device.A first spring 118a is placed in first chamber of this device, and this chamber is defined between an end cap 117 of intermediate body member member 112 and far-end body member 110.A second spring 118b is placed in second chamber of this device, and this chamber is defined between intermediate body member member 112 and near-end body member 114.The first spring 118a passes through a first spring retainer 119a against first lining ring or packing ring 120a fixing.Equally, the second spring 118b passes through a second spring retainer 119b against second lining ring or packing ring 120b fixing.In assembling process, the first spring 118a has first pre-determined compressive force by the progressive joint preload of the first threaded connector 113a, so as by this first spring-compressed between end cap 117 and the first lining ring or packing ring 120a.Please note in this operating process, the first lining ring or packing ring 120a are that the inwall against intermediate body member member 112 is retained on appropriate location.Equally, in assembling process, the second spring 118b has by the progressive joint preload of the second threaded connector 113b second pre-determined compressive force that is greater than this first pre-determined compressive force, so as by this second spring-compressed between intermediate body member member 114 and the second lining ring or packing ring 120b.Please note in this operating process, the second lining ring or packing ring 120b are that the inwall against near-end body member 114 is retained on appropriate location.
Before far-end body member 110 is assembled on intermediate body member member 114, a hoop 122a is placed in a hollow interior part of this intermediate body member member.The object of hoop 122a is, by lining ring or packing ring 120a, the power being provided by the first spring 118a is transferred to a pipeline 6, so that this pipeline is pressed onto in styletable joint 104 by enough power, make like this pressure between this pipeline and this end connector surpass the hydrodynamic pressure of reaching in this post under normal operating condition---for HPLC system, typically be 15000psi(pound/square inch).Because the body of this pipeline is to be become by metal construction generally, therefore binds round a kind of metal of hardness (for example, stainless steel) that 122a is preferably equal to or greater than this pipeline by the hardness having and be configured to.With this selection of material, the power that is applied to hoop 122a in the direction of post 103 will tend to cause that hoop 122a itself gets in this pipeline walls, to produce a kind of metal closely and metal friction sealing.In alternate embodiment, hoop 122a can by pipeline 6 or change with shape of this pipeline all-in-one-piece, this shape is as a ridge, groove, ring etc.In operation, the formed shape part of this pipeline can engage with the holder contacting with the first spring 118a, ring, packing ring, lining ring etc., to spring force is transferred to pipeline 6.
In operation, install 100 and also comprise a deformable containment member 122b, sealing member can be one second hoop, sealing member is placed on pipeline 6 before just in this end of line is positioned to styletable joint 104.The object of deformable containment member 122b is distortion under the applying of the power being provided by lining ring or packing ring 120b by the second spring 118b, to form a kind of leakproof seal between this pipeline, end connector and post.Therefore, deformable containment member 122b is preferably configured to as polyetheretherketone (PEEK) by a kind of elastic polymer material.
When device 100 is not providing in the operation connecting between a pipeline and a post, the spring force of these preloads is to be received in accordingly between the end cap 117 of far-end body member 110 and intermediate body member member 112 and between this intermediate body member member and near-end body member 114.User can comprise a pushing mechanism and a locking or blocking mechanism by the operation 124(of Yi Ge mechanism) by device 100 be placed in operation (at this moment pipeline 6 and bind round 122a in this device in appropriate location and the deformable containment member 122b appropriate location on this pipeline), this mechanism in the direction of fixing post 103 and its end connector 104, promote these three body members (and, therefore, also promote the hardware in supporting member 116, pipeline 6 and these body members).
Once this pipeline contacts with this end connector, further apply (continuation by this promotion and blocking mechanism operates) of power causes that this pipeline applies the power of an increase by binding round 122a and the first lining ring or packing ring 120a for the first spring 118a.Once the reacting force being provided by this pipeline surpasses the spring force of the preload on spring 118a, the continuation operation of this promotion and blocking mechanism will cause this spring-compressed, thereby this device can be moved, so that deformable containment member 122b contacts with near-end body member 114 and styletable joint 104.The further operation of this promotion and blocking mechanism causes the compression of the first spring 118a, together with reacting force the applying by deformable containment member 122b and the second lining ring or packing ring 120b of an increase for the second spring 118b.Still further operating of this promotion and blocking mechanism causes spring 118a, 118b compression, and the spring force that result is applied to this pipeline and this deformable containment member increases.The upper power increasing of deformable containment member 122b and pressure cause these parts in styletable joint 104 and around this pipeline distortion, to produce a kind of leakproof pressure seal.
A dimple 115 can be provided in one end of near-end body member 114, to be provided for, hold a space of deformable containment member 122b and in this promotion and barring program process, guide relatively moving between coupling arrangement 100 and styletable joint 104.Before this device of practical operation, the precompression of these springs has guaranteed to require actual the movement to realize on this pipeline of minimum of part to bind round desired or suitable final power with this deformable containment member or second.
Fig. 4 A-4B be according to originally teach content for a pipeline is attached to a chromatographic column one end two kinds of replacement device the diagram that divides of end.Fig. 4 A, the device 140,145 shown in 4B aspect a lot of with Fig. 3 A in graphic device 100 be similar.Yet, install aspect 140,145 modes that seal for end connector 104,31 and post 103 at pipeline 6 different from device 100.Yet in device 100, deformable containment member 122b can comprise one second hoop simply, in the device 140 shown in Fig. 4 A, a single one, alternative the second lining ring of monomer containment member 123 or packing ring 120b and containment member 122b.Containment member 123 comprises a kind of deformable material to produce a kind of leakproof seal between pipeline 6, end connector 104 and post 103 under the power from the second spring 118b.In the device 140 shown in Fig. 4 A, for this class sealing purpose adopt another one, monomer containment member 125.
Replacement device 145(Fig. 4 B) containment member 125 is modification of the containment member shown in Fig. 4 A, from the outward extending part of near-end body member 114, be wherein elongated, so that can use Novel coupling device 145 that pipeline 6 is attached to conventional styletable joint 31 hermetically.A part that wanders back to many conventional end joints (as conventional end connector 31) by Fig. 2 has ，Gai hole, a hole and has internal thread.This internal thread is designed to match with the external thread of a threaded nut or screw 33, and this nut or screw can rotate to a plus-pressure is provided.Although do not adopt this class screw thread in graphic new embodiment disclosed here, desirable is to combine to adopt the present invention with the existing chromatographic column with conventional joint.Therefore, containment member 125 has an elongated portion, and this elongated portion has a diameter less than the internal diameter of the threaded part of end connector 31.In this way, containment member 125 is adapted to may extend in this routine styletable joint without these screw threads of contact, thereby walks around this threaded portion.Inside from these screw threads, deformable containment member 125 engages with a tapering part in the hole of this routine styletable joint in operation, thereby to form a kind of leakproof seal with deformable containment member or the second hoop 122b for the similar mode of the mode of threadless end connector 104 a kind of sealings of generation (Fig. 3 A).At operative installations 140 or install 145 when a pipeline is disconnected from chromatographic column, sealing member (containment member 123 or containment member 125) can keep being attached to or in near-end body member 114, thereby eliminated user, supply or insert the needs of a hoop when using this corresponding intrument next time.
Coupling arrangement described above adopts two springs separately, and these two springs are if the main axis along corresponding intrument of being considered is with a kind of non-overlapped end-to-end spatial relationship deployment.Yet, if needed, can be by providing a kind of improved hitch designs to save the space along this axial dimension, in this design for example as when a spring, be present at least in part by another spring around space in time, these springs are overlapping along this axial dimension at least in part.An example of a this coupling arrangement shown in Figure 6.
Now in detail referring to coupling arrangement 300, Fig. 6 A and 6C are the first and second perspective external views of the device 300 assembled completely.It is generally acknowledged, install 300 and comprise a housing 302, this housing is the first noumenon member of this device; With a piston 303, this piston is the second body member of this device.Housing 302 has a perforate or chamber 326.Piston 303 can be inserted in the hole or chamber 326 of this housing slidably at least in part, and can be at least in part from this hole or chamber retract.Preferably, the part in hole or chamber 326 comprises the shape matching with this part of this piston, and this part of this piston can be inserted in this hole or chamber slidably.If this part of this piston is columniform, this piston and hole can be said to be a kind of piston-cylinder relation that comprises so.
A lining ring or other bearings 311 can be provided in this part in hole or chamber 326, and this lining ring or other bearings receive this part of piston 303 to be provided for a smooth slipping plane of insertion and the retraction of this piston.Can by a kind of, promote and locking (or locking) mechanism 324 manually controls this piston and moves in this housing or partially retracted from this housing of this piston by user.As shown, this promotion and blocking mechanism can comprise that a manual manipulation handle 321 and a pitman shaft 325 are so that pitman shaft 325 mechanically engages with handle 321 by first pivot pin 322, and one end of this pitman shaft is freely around this pivot pin rotation.Second pivot pin (not shown) provides mechanical engagement similarly between the opposite end of pitman shaft 325 and piston 303, so that rotatablely moving of handle 321 is converted to the translation motion of this piston.
Among piston 303, have a chamber 327, the pipeline 306 of certain length is through this chamber.The illustration 330 of Fig. 3 B illustrates the zoomed-in view of a part for device 300, so that can see that an end of pipeline 306 divides outside an end plate 312 that is projected into piston 303.A containment member 323(sealing member is a part for this device and can is a deformable hoop) around the part of pipeline 306 so that the end portions of this pipeline be projected into outside containment member 323.Containment member 323 has a conical outer surface, this conical outer surface is designed to that it needs not to be parts of device 300 with a conventional end joint 304b(, but be illustrated for the sake of clarity) cone-shaped inner surface match, to a kind of leakproof seal is provided in end connector 304b.In operation, end connector 304b generally will be installed on one end of a chromatographic column (not shown), and this end is by the inlet end or the endpiece that are this post.Therefore,, when pipeline 306 and containment member 323 are inserted in end connector 304b by coupling arrangement 300, pipeline 306 delivers a fluid in this chromatographic column or receives the fluid from this chromatographic column.
In the view shown in Fig. 6 A, 6B, this coupling arrangement be illustrated in an open position so that the end of pipeline 306 is retracted from end connector 304b.At this, open in configuration, this chromatographic column can be removed or change.Then same or a different chromatographic column can be positioned to correct position, so that by its end connector being positioned to the end that receives pipeline 306 in a line of rabbet joint, dimple or the groove 307 of housing 302.Therefore it is identical with the post supporting member 106 of discussing in conjunction with other embodiment at this above functional that a part that, comprises this housing of this line of rabbet joint, dimple or groove 307 provides.Subsequently, operation promotion and blocking mechanism 324 are to cause that piston 303 is moved further to hole or chamber 326, and now pipeline 306 is along with this motion delivers, until this end of line engages with end connector 304b.This handle causes between this pipeline and this end connector and forms a kind of leakproof seal in mode described below in unidirectional further operation.
Fig. 6 C is by installing 300 piston 303 center and by the viewgraph of cross-section at pipeline 306 center, and this figure illustrates the internal part in the chamber 327 of this piston.Fig. 6 C also illustrates housing 302 can be fixed to a base plate or external shell 308, so that as the above position stability effect about the generator 300 that other embodiment discussed.These parts in chamber 327 make device 300 between pipeline 306 and chromatographic column end connector 304b, provide a kind of leakproof seal, or even under the high pressure running in HPLC application, and do not need a user to adopt an instrument or this pipeline or this post are applied to any twisting motion or moment of torsion.
As observed from Fig. 6 C, in piston chamber 327, be mounted with a first volute spring 318a and a second volute spring 318b, the internal diameter that this second spring has is greater than the external diameter of this first spring.Settle these springs, so that they are overlapping at least in part---, so that in volume or space that at least a portion of the first spring 318a is present in that internal diameter by the second spring 318b defines.Pipeline 306 is parallel to haply and along these two spring 318a, the coaxial line of 318b passes.In operation, first volute spring 318a(Fig. 6 C) collar, sleeve pipe or the flange 319 by the one end in abutting connection with this first spring transfers to pipeline 306 by first spring force.The collar, sleeve pipe or flange 319 are be fixed to pipeline 306 or closely engage with this pipeline, to be parallel to haply its axis and in the direction of end connector 304b, this pipeline applied to a power.Be screwed into the screw 317 of a part of piston chamber 327 in abutting connection with the other end of this first spring and can be by preconditioning to the force of compression of a desirable preload is provided to this spring.The second volute spring 318b by push pedal 320(in the middle of as a lining ring or a flange) second spring force is transferred to containment member 323.The push pedal 320 of the second spring 318b by that end nearest apart from end connector 304b and an inwall 329 by this chamber at the other end are limited in piston chamber 327.Push pedal 320 is limited in chamber 327 for these spring forces by one or more mechanical stop members 328, and this or these mechanical stop member is to be engaged to one or more piston walls and can to comprise one group of pin, locking ring or a flange fixing by an inside groove in an inner carrier wall that for example passes a plurality of holes in this piston wall or any other boss or the protuberance that is engaged to or is fixed to this piston.When this promotion and blocking mechanism are in an open position so that pipeline 306 while retracting from end connector 304b, this or these mechanical stop member prevent these springs by them own and/or miscellaneous part release outside chamber 327.
Fig. 6 D is the skeleton view of isolated pipeline 306.In this exemplary embodiment, pipeline 306 is custom-designed pipelines, and it is designed to engage with the collar, sleeve pipe or flange 319, to be therefore mechanically attached to the first volute spring 318a.As shown, this pipeline comprises the outer radius portion 316 of one or more increases, between the outer radius portion of this or these increase, defines a groove 314, and in operation, this groove engages with a part for the collar, sleeve pipe or flange 319.The end portion of pipeline 306 preferably includes a nominal outer diameter to become exercisable conventional end joint or pipeline jointing.Those of ordinary skill in the art will be understood that, alternately, can eliminate groove 314 and be fixed to a pipeline all the time with normal diameter to be conducive to a flange or ring.Under this alternative case, the flange that this is fixing or ring are parts 319.
In the routine operation of this exemplary means embodiment, pipeline 306 keeps together with this device 300 all the time at some joints of device with in being disengaged the whole process of operation, and wherein this generic operation is with some removals of for example one or more chromatographic columns and changes to operate and be associated.End at this pipeline relative with filter cylinder, pipeline 306 can be connected on the conventional chromatogram pipeline (not shown) of certain length by a conventional pipeline jointing 304a, and this routine pipeline jointing comprises that a coupling nut 301, a tubulose connect body 305 and a hoop 309.The situation that this routine pipeline (or pipeline 306) must be replaced is more not frequent by the situation that is conventionally removed, adds or change than a post.In those moment that must change conventional pipeline or pipeline 306, can by disconnecting pipeline jointing 304a, complete with standard mode connection and the disconnection of this length of pipeline.Yet, use the device of this disclosure, exist in no instance use an erecting tools apply a twisting motion or moment of torsion to any requirement of this chromatographic column or its end connector.
In the operation of coupling arrangement 300, after the jag of pipeline 306 contacts with end connector 304b, due to this pipeline character fixing with respect to the position of this housing, handle 321 can not cause this pipeline being moved further with respect to end connector 304b or this housing unidirectional being moved further.On the contrary, the continuation movement of this handle and piston causes the other compression of the first spring 318a, so that be applied to pipeline 306 from the spring force of this first spring in the direction towards this end connector.Owing to being previously applied to the preload compression of this first spring, the pressure between this pipeline and this end connector is increased to and moves to its simultaneous certain resulting pressure of final locked position of coupler with handle 321 from zero rapidly.Resulting pressure between this pipeline and this end connector is like this so that surpasses the hydrodynamic pressure of reaching under normal operating condition in this post---for HPLC system, typically be 15000psi.Simultaneously, may contact and start after a while with end connector 304b at pipeline 306, piston inner wall 329 moves against the second spring 318b and causes that the spring force of increase is applied to containment member 323 by push pedal 320, so that distortion under the applying of the power being provided by the second spring 318b, to form a kind of leakproof seal between this pipeline, end connector and post.Therefore, deformable containment member 323 is preferably configured to as polyetheretherketone (PEEK) by a kind of elastic polymer material.
Usually, desired or desired be to carry out join operation at the two ends of a chromatographic column.For example, when changing a post, the connection of locating at the two ends of the post being replaced must disconnect and form and connect at the place, two ends that changes post.Therefore, Fig. 5 diagram according to originally teach content for an intake pipeline and export pipeline being attached to a system of the corresponding end of a chromatographic column.Particular system 150 shown in Fig. 5 can with coupling arrangement 100(Fig. 3 A), 140(Fig. 4 A) or 145(Fig. 4 B) in any or use together with similar coupling arrangement.System 150 comprises a single pedestal or housing 108, this pedestal or housing by one first slidably supporting member 116a and one second slidably supporting member 116b at the place, opposite end of a chromatographic column 77, support a first coupling arrangement 100a and a second coupling arrangement 100b accordingly.This post with respect to pedestal or housing 108 by being supported in fixed position at the input end of post 77 and post supporting member 106a and the 106b of output accordingly.Slidably supporting member (116a, 116b), column holder (106a, 106b) and coupling arrangement (100a,, be 100b) similar to the like of describing with respect to Fig. 3 A to 3B above.Therefore the coupling arrangement 100a that, comprises far-end body member 110a, intermediate body member member 112a and near-end body member 116a is for being attached to an intake pipeline 6d input end of post 77.Equally, the coupling arrangement 100b that comprises far-end body member 110b, intermediate body member member 112b and near-end body member 116b is for being attached to an export pipeline 6e output terminal of post 77.First promotion and locking or locking mechanism 130a and second promotion and locking or locking mechanism 130b can operate to compression movement described above is provided by user separately.
Directly and easily use two coupling arrangements 300 as shown in Fig. 6 A to 6C or the functional system providing is as shown in Figure 5 provided with similar device.Because each coupling arrangement 300 provides a built-in supporting construction for a styletable joint and one piston slidably, it is unnecessary therefore causing the slidably supporting member (116a, 116b) shown in Fig. 5 and column holder (106a, 106b).Desired all be by device two examples of 300 face each other, apart one section of suitable distance is attached on a base plate or is attached at external shell 308(referring to Fig. 6 C) on or within so that two end connectors of a chromatographic column are easily engaged in the line of rabbet joint, dimple or the groove of these two coupling arrangements.In order to adapt to the post of different length, what may wish is, in slidably a kind of or other adjustable mode, at least one in these coupling arrangements is attached to this base plate or housing, so that can regulate the distance between these two coupling arrangements.
Fig. 7 be according to originally teach content for pipeline being attached to the process flow diagram of a kind of method (method 200) of a chromatographic column.Method 200 is particularly useful for the A in conjunction with coupling arrangement 100(Fig. 3), 140(Fig. 4 A) or 145(Fig. 4 B) in any or similar coupling arrangement use.The first step (step 202) of method 200 comprises a part for one section of chromatogram pipeline (comprising an end of line) through a coupling arrangement, this coupling arrangement comprises: deformable containment member and have at least one chamber of first spring and second spring, wherein this first spring can transfer to this chromatogram pipeline from this first spring by first spring force, and wherein this deformable containment member can receive a power from this second spring.If this pipeline and device have been used in a previous linker, can remove so the behavior through this device by this part of this section of chromatogram pipeline.In this case, this step becomes only provides the step having through the described device of this pipeline of this device.
In next step (step 206), this end of line is inserted in a hollow body of this chromatographic column end connector.Then, in step 208, towards this chromatographic column, move this coupling arrangement, so that this first spring drives this pipeline to proceed in this container by a power, this power produces this end of line for a pressure of this end connector, and this pressure surpasses when the maximum operating pressure lower time of this post in it is by the hydrodynamic pressure of generation.In step 210, continue this Quick Connect Kit towards the movement of this chromatographic column, so that causing this deformable containment member, this second spring forms a kind of Fluid Sealing between this post and this container, to prevent any seepage under this maximum operating pressure.
After a post has been connected to as described chromatogram pipeline and has been used with this connection configuration, opening operation is simple---user with for being connected in the direction of opposite direction of this post and pipeline, operate simply this promotion and blocking mechanism.This opening operation can be as promoted simply a handle to discharge applied force and make this device be disengaged the same simple with pipeline from this styletable joint on oppositely.How the those of ordinary skill of mechanical field provides one of this reverse operating of execution to promote and blocking mechanism by easily understanding.
Disclosed for pipeline being attached to the improved apparatus and method of a chromatographic column.Use disclosed device, user can be placed into a part for a post and pipeline (comprising an end of line) in this device and operate a pushing mechanism simply, as a handle.As described above, the location of this pipe and styletable joint, is guaranteed by this device together with applying of suitable power.Due to the retain strength on this pipe be with sealing member (it can be a hoop) on sealing force separate, so each power can only arrange where necessary, thereby makes to compare with the typical combination of pipeline and hoop, this pipe and hoop can be reused more times.This device eliminated demand to being applied to the twisting motion of this post or pipeline, highly reproducible connection and opening operation are provided and motion and the mechanical advantage of appropriate amount are provided, so that operator does not need an instrument, for a post being connected to pipeline or a post being disconnected from pipeline.
The content of the discussions comprising in the application is intended to as basic explanation.Although describe the present invention according to different embodiment shown and that describe, those skilled in the art will readily recognize that the change that can exist these embodiment, and these changes will be within the spirit and scope of the present invention.Reader it will be appreciated that these concrete discussion may not describe all possible embodiment clearly; Many replacement schemes imply.Therefore,, in the situation that not departing from spirit of the present invention, scope and essence, those of ordinary skills can make many modifications.Description and term are not all intended to limit the scope of the invention.All patented claim disclosure contents, Patent Application Publication or other are openly combined in this (as fully set forth at this) hereby by reference clearly.
Claims (29)
1. for chromatographic column being attached to a device for pipeline, this chromatographic column is liquid-phase chromatographic column and comprises an end connector, and this pipeline has certain length, and this device comprises:
At least one body member, this at least one body member comprises at least one chamber;
First spring in this at least one chamber, this first spring is configured to apply first power to the pipeline of this length towards this styletable joint;
Second spring in this at least one chamber, this second spring is configured to apply on second power to an one deformable containment member towards this styletable joint; And
One promotes and blocking mechanism, and this promotion and blocking mechanism are configured to promote this at least one body member, this first spring and this second spring towards this styletable joint.
2. device as claimed in claim 1, further comprise a movably supporting member, this supporting member is fixed to this at least one body member, and wherein this promotion and blocking mechanism are further configured to towards this styletable joint and promote this movably supporting member.
3. device as claimed in claim 2, wherein this at least one body member comprises:
A near-end body member, this near-end body member is fixed to this movably supporting member and have and be configured to the first end and second end that coordinate with this styletable joint;
An intermediate body member member, this intermediate body member member comprises the intermediate body member member first end and intermediate body member member second end that are attached to this second end of this near-end body member; And
A far-end body member, this far-end body member is attached to this intermediate body member member the second end,
Wherein this at least one chamber comprises first chamber and second chamber, this first chamber comprises this first spring and by this intermediate body member member and this far-end body member gauge, and this second chamber comprises this second spring and by this intermediate body member member and this near-end body member gauge.
4. device as claimed in claim 3, wherein this far-end body member is to be attached to this intermediate body member member and this intermediate body member member is to be attached to this near-end body member by a second threaded connector by a first threaded connector, and wherein this first and second threaded connector is configured to make this first and second spring to remain on accordingly under the first and second pre-determined compressive force.
5. the device as described in any one in claim 1 to 4, wherein this first spring is configured to make this first power can surpass a reaction current muscle power with a maximum column operation pressure correlation connection, and wherein this second spring be configured to make when this post be that this second power can cause that this deformable containment member forms a kind of leakproof seal for this styletable joint under this maximum column operation pressure time.
6. device as claimed in claim 1 or 2, wherein sealing member comprises a hoop.
7. device as claimed in claim 5, wherein sealing member comprises a hoop.
8. the device as described in claim 3 or 4, wherein sealing member comprises a hoop.
9. device as claimed in claim 8, further comprises:
One second hoop, this second hoop is in a hollow interior part of this intermediate body member member; And
First lining ring or packing ring, this first lining ring or packing ring are placed between this first spring and this second hoop,
Wherein this second hoop and this first lining ring or packing ring are configured to make this first spring to apply this first power to the pipeline of this length with this second hoop by this first lining ring or packing ring.
10. device as claimed in claim 9, wherein this second hoop is to be formed by a kind of material, this material has the hardness identical with a kind of wall material of the pipeline of this length or harder than a kind of wall material of the pipeline of this length.
11. devices as claimed in claim 9, wherein this second hoop is to be formed by stainless steel.
12. devices as claimed in claim 2, further comprise:
Pedestal or housing, this movably supporting member be attached to slidably this pedestal or housing; And
A post supporting member, for remaining on a fixing position by this liquid-phase chromatographic column with respect to this pedestal or housing.
13. devices as claimed in claim 12, wherein this movably supporting member be to be attached to slidably this pedestal or housing by a guide rail.
14. devices as claimed in claim 1, wherein this at least one body member comprises a piston and further comprises:
A housing, this housing comprises:
A supporting construction, for supporting this end connector; And
Hole or ，Gai hole, chamber or chamber have a part, by this promotion and blocking mechanism, are promoting in this Piston Process, and a part for this piston moves slidably in this part.
15. devices as claimed in claim 14, wherein at least a portion of this first spring is to be placed in this second spring.
16. devices as claimed in claim 14, wherein this first spring is a volute spring with an external diameter, wherein this second spring is a volute spring with an internal diameter, and wherein this first spring is to be placed in the volume that this internal diameter by this second spring defines.
17. devices as described in any one in claim 14 to 16, further comprise the collar with a flange, and this flange matches for power is transferred to this pipeline from this first spring with a groove in this pipeline.
18. devices as described in any one in claim 14 to 16, further comprise a flange that is arranged on this pipeline or is fixed to this pipeline, for power is transferred to this pipeline from this first spring.
19. devices as claimed in claim 14, further comprise and are screwed into a part for this chamber and can operate with a screw to a predetermined compression of this first spring supply.
20. devices as claimed in claim 14, wherein this first spring is configured to make this first power can surpass a reaction current muscle power with a maximum column operation pressure correlation connection, and wherein this second spring be configured to make when this post be that this second power can cause that this deformable containment member forms a kind of leakproof seal for this styletable joint under this maximum column operation pressure time.
21. 1 kinds for being attached to chromatographic column in the device of pipeline, this chromatographic column is liquid-phase chromatographic column and comprises a first end with a first end joint and second end with second end connector, this pipeline comprises the first pipeline and the second pipeline, and this device comprises:
A pedestal; And
Be fixed to first and second coupling arrangement of this pedestal, each coupling arrangement is used for one of this first and second end connector one of being attached in this first and second pipeline, and each coupling arrangement comprises:
At least one body member, this at least one body member comprises at least one chamber;
First spring in this at least one chamber, this first spring is configured to apply first power to coupled pipeline towards coupled end connector;
Second spring in this at least one chamber, this second spring is configured to apply second power to an one deformable containment member towards coupled end connector; And
One promotes and blocking mechanism, and this promotion and blocking mechanism are configured to promote this at least one body member, this first spring and this second spring towards this post.
22. devices as claimed in claim 21, wherein at least one in this first and second coupling arrangement further comprises:
A supporting member movably, this supporting member is fixed to this at least one body member, and wherein this promotion and blocking mechanism are further configured to towards coupled end connector and promote this movably supporting member;
A near-end body member, this near-end body member is fixed to this movably supporting member and have and be configured to the first end and second end that match with coupled end connector;
An intermediate body member member, this intermediate body member member comprises the intermediate body member member first end and intermediate body member member second end that are attached to this second end of this near-end body member; And
A far-end body member, this far-end body member is attached to this intermediate body member member the second end,
Wherein this at least one chamber comprises first chamber and second chamber, this first chamber comprises this first spring and by this intermediate body member member and this far-end body member gauge, and this second chamber comprises this second spring and by this intermediate body member member and this near-end body member gauge.
23. devices as claimed in claim 21, wherein at least one in this first and second coupling arrangement further comprises:
A housing, this housing comprises:
A supporting construction, for supporting coupled end connector; And
Hole or chamber, this hole or chamber have a part, by this promotion and blocking mechanism, promoting in the process of this at least one body member, a part for this at least one body member moves slidably in this part, and wherein this at least one body member comprises a piston.
24. devices as claimed in claim 23, wherein at least a portion of this first spring is to be placed in this second spring.
25. devices as claimed in claim 23, wherein this first spring is a volute spring with an external diameter, wherein this second spring is a volute spring with an internal diameter, and wherein this first spring is to be placed in the volume that this internal diameter by this second spring defines.
26. devices as described in any one in claim 23 to 25, further comprise the collar with a flange, and this flange matches for power is transferred to coupled pipeline from this first spring with a groove in coupled pipeline.
27. devices as described in any one in claim 23 to 25, further comprise a flange that is arranged on this pipeline or is fixed to coupled pipeline, for power is transferred to coupled pipeline from this first spring.
28. devices as claimed in claim 23, further comprise and are screwed into a part for this chamber and can operate with a screw to a predetermined compression of this first spring supply.
29. devices as claimed in claim 23, wherein this first spring is configured to make this first power can surpass a reaction current muscle power with a maximum column operation pressure correlation connection, and wherein this second spring be configured to make when this post be that this second power can cause that this deformable containment member forms a kind of leakproof seal for coupled end connector under this maximum column operation pressure time.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40803910P | 2010-10-29 | 2010-10-29 | |
| US61/408,039 | 2010-10-29 | ||
| PCT/US2011/058226 WO2012058513A1 (en) | 2010-10-29 | 2011-10-28 | Apparatus and method for coupling tubing to chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203479771U true CN203479771U (en) | 2014-03-12 |
Family
ID=45994417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201190000861.XU Expired - Lifetime CN203479771U (en) | 2010-10-29 | 2011-10-28 | Device for connecting chromatographic column to pipeline |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20150209787A1 (en) |
| EP (2) | EP3128322B1 (en) |
| CN (1) | CN203479771U (en) |
| WO (1) | WO2012058513A1 (en) |
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| CN105761912A (en) * | 2016-05-16 | 2016-07-13 | 国网新疆电力公司阿克苏供电公司 | Matching structure for ensuring reliable contact of head portion of sleeve and carrying current |
| CN105987976A (en) * | 2016-07-01 | 2016-10-05 | 大连技嘉科技有限公司 | Column oven capable of rapidly replacing chromatographic column |
| CN106461627A (en) * | 2014-05-22 | 2017-02-22 | 株式会社岛津制作所 | Column-attaching device and ferrule set |
| CN106461626A (en) * | 2014-05-16 | 2017-02-22 | 株式会社岛津制作所 | Column attachment device |
| CN106471303A (en) * | 2014-05-07 | 2017-03-01 | 沃特世科技公司 | There is the crimp contact of connection lasso |
| CN109073610A (en) * | 2016-05-17 | 2018-12-21 | 株式会社日立高新技术 | Splitter attachment device, connection method and analysis system |
| CN111051875A (en) * | 2017-09-14 | 2020-04-21 | 株式会社岛津制作所 | Liquid chromatograph |
| CN111649050A (en) * | 2020-05-22 | 2020-09-11 | 中国科学院广州能源研究所 | Gas chromatography column connector |
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| WO2008073535A2 (en) * | 2006-08-12 | 2008-06-19 | Van Pelt Colleen K | Nanoliter flow rate separation and electrospray device with plug and play high pressure connections and multi-sensor diagnostic monitoring system |
| US10338038B2 (en) | 2011-12-21 | 2019-07-02 | Waters Technologies Corporation | Establishing fluidic connections between chromatography components |
| CN104105963B (en) * | 2012-02-01 | 2017-08-08 | 安捷伦科技有限公司 | For the quick-lock connector for the fluid conduit systems that capillary is connected to fluid assembly |
| CN104781665B (en) * | 2012-11-21 | 2016-08-31 | 株式会社日立高新技术 | Pipe arrangement connection joint |
| JP6329128B2 (en) * | 2013-03-11 | 2018-05-23 | 積水メディカル株式会社 | Column apparatus for liquid chromatography and liquid chromatography apparatus |
| WO2017075129A1 (en) * | 2015-10-28 | 2017-05-04 | Ultra Clean Technology | Precision connections and methods of forming same |
| DE102017106089B4 (en) * | 2016-04-11 | 2023-05-25 | Micromass Uk Limited | PROBE ASSEMBLY CONNECTION |
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| US10422777B1 (en) * | 2017-12-29 | 2019-09-24 | Bio-Rad Laboratories, Inc. | Clamp for chromatography columns |
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| WO2021222055A1 (en) | 2020-04-29 | 2021-11-04 | Waters Technologies Corporation | Establishing fluidic connections between chromatography components |
| CN114113431A (en) * | 2020-08-28 | 2022-03-01 | 萨默费尼根有限公司 | Connector for gas chromatography column |
| EP4308263A1 (en) | 2021-03-16 | 2024-01-24 | Bio-Rad Laboratories, Inc. | Dynamic axial compression for preparative columns using external compression |
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- 2011-10-28 WO PCT/US2011/058226 patent/WO2012058513A1/en active Application Filing
- 2011-10-28 US US13/882,048 patent/US20150209787A1/en not_active Abandoned
- 2011-10-28 CN CN201190000861.XU patent/CN203479771U/en not_active Expired - Lifetime
- 2011-10-28 EP EP16189843.2A patent/EP3128322B1/en active Active
- 2011-10-28 EP EP11837141.8A patent/EP2633304B1/en active Active
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2017
- 2017-04-26 US US15/498,340 patent/US20170227507A1/en not_active Abandoned
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106471303B (en) * | 2014-05-07 | 2019-11-19 | 沃特世科技公司 | Crimp contact with connection lasso |
| CN106471303A (en) * | 2014-05-07 | 2017-03-01 | 沃特世科技公司 | There is the crimp contact of connection lasso |
| CN106461626A (en) * | 2014-05-16 | 2017-02-22 | 株式会社岛津制作所 | Column attachment device |
| CN106461626B (en) * | 2014-05-16 | 2018-06-26 | 株式会社岛津制作所 | Tubing string erecting device |
| CN106461627A (en) * | 2014-05-22 | 2017-02-22 | 株式会社岛津制作所 | Column-attaching device and ferrule set |
| CN105761912A (en) * | 2016-05-16 | 2016-07-13 | 国网新疆电力公司阿克苏供电公司 | Matching structure for ensuring reliable contact of head portion of sleeve and carrying current |
| CN109073610A (en) * | 2016-05-17 | 2018-12-21 | 株式会社日立高新技术 | Splitter attachment device, connection method and analysis system |
| CN109073610B (en) * | 2016-05-17 | 2020-10-16 | 株式会社日立高新技术 | Separation column connection device, connection method and analysis system |
| CN105987976A (en) * | 2016-07-01 | 2016-10-05 | 大连技嘉科技有限公司 | Column oven capable of rapidly replacing chromatographic column |
| CN111051875A (en) * | 2017-09-14 | 2020-04-21 | 株式会社岛津制作所 | Liquid chromatograph |
| CN111051875B (en) * | 2017-09-14 | 2022-06-28 | 株式会社岛津制作所 | Liquid chromatograph |
| CN111649050A (en) * | 2020-05-22 | 2020-09-11 | 中国科学院广州能源研究所 | Gas chromatography column connector |
| CN111649050B (en) * | 2020-05-22 | 2021-07-20 | 中国科学院广州能源研究所 | Gas chromatography column connector |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3128322B1 (en) | 2023-09-06 |
| EP2633304B1 (en) | 2016-12-07 |
| EP3128322A1 (en) | 2017-02-08 |
| EP2633304A1 (en) | 2013-09-04 |
| US20170227507A1 (en) | 2017-08-10 |
| EP2633304A4 (en) | 2015-07-22 |
| WO2012058513A1 (en) | 2012-05-03 |
| US20150209787A1 (en) | 2015-07-30 |
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Legal Events
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140312 |