CN203403089U - Collector for obtaining nucleic acid clinical specimen - Google Patents
Collector for obtaining nucleic acid clinical specimen Download PDFInfo
- Publication number
- CN203403089U CN203403089U CN201320106733.4U CN201320106733U CN203403089U CN 203403089 U CN203403089 U CN 203403089U CN 201320106733 U CN201320106733 U CN 201320106733U CN 203403089 U CN203403089 U CN 203403089U
- Authority
- CN
- China
- Prior art keywords
- tube
- nucleic acid
- collector
- strainer tube
- filter membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 239000012530 fluid Substances 0.000 claims description 14
- 238000004090 dissolution Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 5
- 239000000443 aerosol Substances 0.000 description 3
- 238000010241 blood sampling Methods 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a collector for obtaining a nucleic acid clinical specimen, comprising an air collection tube, a filter tube and a dissolved liquid collection tube. The collector is characterized in that the air collection tube, the filter tube and the dissolved liquid collection tube are orderly connected in an insertion manner from top to bottom; the filter tube is internally provided with a filter membrane, and the filter membrane is placed on a mesh bracket; the bottom end of the filter tube is provided with an opening, a sealing cover is arranged at the opening, and the top end of the filter tube is provided with a tube cap.
Description
Technical field
The utility model relates to a kind of for obtaining the collector of nucleic acid clinical samples.
Background technology
When medical science detects, often need to gather patient's nucleic acid clinical samples.Existing acquisition method mostly is and gathers peripheral blood in patients 3-5 milliliter or tissue sample 1-2mg, by test kit, extracts nucleic acid, obtains nucleic acid clinical samples.But also do not have good method to collect and extract free nucleic acid sample in aerosol.
Utility model content
Goal of the invention of the present utility model is to be provided for obtaining the collector of nucleic acid clinical samples, does not need blood sampling, easy and simple to handle, and collection effect is good.。
Realize the technical scheme of the present utility model object:
For obtaining a collector for nucleic acid clinical samples, comprise air trapping pipe, strainer tube and dissolution fluid collection tube, it is characterized in that: air trapping pipe, strainer tube and dissolution fluid collection tube are from top to bottom pegged graft successively; In strainer tube, be provided with filter membrane, filter membrane is placed on netted bracket; The bottom of strainer tube is provided with opening, and opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid.
The top of air trapping pipe, the bottom of strainer tube are all tapered.
The opening of strainer tube bottom is threaded with closing cap.
Filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.
The beneficial effect the utlity model has:
The nucleic acid material of microorganism or its release forms aerosol in air, and when aerosol passes through filter membrane, macromole nucleic acid is wherein trapped within on filter membrane.With deionized water or TE damping fluid, infiltrate filter membrane, by ultrasonic, by the gleanings stripping on filter membrane, collect dissolution fluid and obtain nucleic acid clinical samples.The utility model does not need blood sampling, only needs patient to exhale and can realize the collection of nucleic acid clinical samples, simple to operate, quick, efficient, without wound, and the utility model not only can be applicable to the medical science detections such as tubercule bacillus detections, can also be widely used in environmental monitoring.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of the utility model strainer tube.
Embodiment
As shown in Figure 1, gathering device comprises air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3, and air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3 are from top to bottom pegged graft successively, between the bottom of strainer tube and the bottom of dissolution fluid collection tube, leave space.The gas that air trapping pipe 1 is breathed out for receiving patient, also can be connected with other air feed equipments.The bottom of the top of air trapping pipe 1, strainer tube 2 is all tapered.As shown in Figure 2, be provided with filter membrane 5 in strainer tube, filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.Filter membrane 5 is placed on bracket 6, and bracket 6 is reticulated structure, and filter membrane 5 is played a supporting role, and prevents that filter membrane 5 from coming off.The top of strainer tube is provided with pipe and covers 4, the bottom of strainer tube is provided with opening, opening part is provided with closing cap 7, the open outer side of strainer tube bottom is provided with outside screw, closing cap inner side is provided with internal thread, strainer tube bottom end opening is threaded with closing cap, the opening of strainer tube bottom also can with the equipment connections such as negative pressure lead device.
While gathering nucleic acid sample, air trapping pipe 1, strainer tube 2 are pegged graft, now do not use closing cap 7; After patient is deeply air-breathing, with air trapping duct occlusion patient oral area, patient's forced expiration, repeats 3 times, collects 5-10L patient and exhales; Then screw closing cap 7, remove air trapping pipe 1, strainer tube 2 is inserted in dissolution fluid collection tube; To the TE damping fluid or the deionized water that add 800 microlitres in strainer tube, lid upper tube cap, centrifugal 10 minutes of 1000g, makes liquid complete wetting filter membrane 5; Open pipe and cover 4, insert ultrasound probe, ultrasonic the gleanings stripping on filter membrane 5 through the short period of time; Screw off closing cap 7, lid upper tube cap 4, puts into a new dissolution fluid collection tube by chimney filter; 13000g high speed centrifugation 10 minutes, the dissolution fluid of collecting in dissolution fluid collection tube is determined nucleic acid sample, cryopreservation.
Claims (4)
1. for obtaining a collector for nucleic acid clinical samples, comprise air trapping pipe, strainer tube and dissolution fluid collection tube, it is characterized in that: air trapping pipe, strainer tube and dissolution fluid collection tube are from top to bottom pegged graft successively; In strainer tube, be provided with filter membrane, filter membrane is placed on netted bracket; The bottom of strainer tube is provided with opening, and opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid.
2. according to claim 1 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: the top of air trapping pipe, the bottom of strainer tube are all tapered.
3. according to claim 2 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: the opening of strainer tube bottom is threaded with closing cap.
4. according to claim 3 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201320106733.4U CN203403089U (en) | 2013-02-25 | 2013-02-25 | Collector for obtaining nucleic acid clinical specimen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201320106733.4U CN203403089U (en) | 2013-02-25 | 2013-02-25 | Collector for obtaining nucleic acid clinical specimen |
Publications (1)
Publication Number | Publication Date |
---|---|
CN203403089U true CN203403089U (en) | 2014-01-22 |
Family
ID=49939027
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201320106733.4U Expired - Lifetime CN203403089U (en) | 2013-02-25 | 2013-02-25 | Collector for obtaining nucleic acid clinical specimen |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN203403089U (en) |
-
2013
- 2013-02-25 CN CN201320106733.4U patent/CN203403089U/en not_active Expired - Lifetime
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
AV01 | Patent right actively abandoned |
Granted publication date: 20140122 Effective date of abandoning: 20151125 |
|
C25 | Abandonment of patent right or utility model to avoid double patenting |