CN203403089U - Collector for obtaining nucleic acid clinical specimen - Google Patents

Collector for obtaining nucleic acid clinical specimen Download PDF

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Publication number
CN203403089U
CN203403089U CN201320106733.4U CN201320106733U CN203403089U CN 203403089 U CN203403089 U CN 203403089U CN 201320106733 U CN201320106733 U CN 201320106733U CN 203403089 U CN203403089 U CN 203403089U
Authority
CN
China
Prior art keywords
tube
nucleic acid
collector
strainer tube
filter membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn - After Issue
Application number
CN201320106733.4U
Other languages
Chinese (zh)
Inventor
张凤民
宋武琦
李玉军
蔡文辉
赵宏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harbin Engineering University
Harbin Medical University
Original Assignee
Harbin Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harbin Medical University filed Critical Harbin Medical University
Priority to CN201320106733.4U priority Critical patent/CN203403089U/en
Application granted granted Critical
Publication of CN203403089U publication Critical patent/CN203403089U/en
Anticipated expiration legal-status Critical
Withdrawn - After Issue legal-status Critical Current

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Abstract

The utility model relates to a collector for obtaining a nucleic acid clinical specimen, comprising an air collection tube, a filter tube and a dissolved liquid collection tube. The collector is characterized in that the air collection tube, the filter tube and the dissolved liquid collection tube are orderly connected in an insertion manner from top to bottom; the filter tube is internally provided with a filter membrane, and the filter membrane is placed on a mesh bracket; the bottom end of the filter tube is provided with an opening, a sealing cover is arranged at the opening, and the top end of the filter tube is provided with a tube cap.

Description

For obtaining the collector of nucleic acid clinical samples
Technical field
The utility model relates to a kind of for obtaining the collector of nucleic acid clinical samples.
Background technology
When medical science detects, often need to gather patient's nucleic acid clinical samples.Existing acquisition method mostly is and gathers peripheral blood in patients 3-5 milliliter or tissue sample 1-2mg, by test kit, extracts nucleic acid, obtains nucleic acid clinical samples.But also do not have good method to collect and extract free nucleic acid sample in aerosol.
Utility model content
Goal of the invention of the present utility model is to be provided for obtaining the collector of nucleic acid clinical samples, does not need blood sampling, easy and simple to handle, and collection effect is good.。
Realize the technical scheme of the present utility model object:
For obtaining a collector for nucleic acid clinical samples, comprise air trapping pipe, strainer tube and dissolution fluid collection tube, it is characterized in that: air trapping pipe, strainer tube and dissolution fluid collection tube are from top to bottom pegged graft successively; In strainer tube, be provided with filter membrane, filter membrane is placed on netted bracket; The bottom of strainer tube is provided with opening, and opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid.
The top of air trapping pipe, the bottom of strainer tube are all tapered.
The opening of strainer tube bottom is threaded with closing cap.
Filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.
The beneficial effect the utlity model has:
The nucleic acid material of microorganism or its release forms aerosol in air, and when aerosol passes through filter membrane, macromole nucleic acid is wherein trapped within on filter membrane.With deionized water or TE damping fluid, infiltrate filter membrane, by ultrasonic, by the gleanings stripping on filter membrane, collect dissolution fluid and obtain nucleic acid clinical samples.The utility model does not need blood sampling, only needs patient to exhale and can realize the collection of nucleic acid clinical samples, simple to operate, quick, efficient, without wound, and the utility model not only can be applicable to the medical science detections such as tubercule bacillus detections, can also be widely used in environmental monitoring.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of the utility model strainer tube.
Embodiment
As shown in Figure 1, gathering device comprises air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3, and air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3 are from top to bottom pegged graft successively, between the bottom of strainer tube and the bottom of dissolution fluid collection tube, leave space.The gas that air trapping pipe 1 is breathed out for receiving patient, also can be connected with other air feed equipments.The bottom of the top of air trapping pipe 1, strainer tube 2 is all tapered.As shown in Figure 2, be provided with filter membrane 5 in strainer tube, filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.Filter membrane 5 is placed on bracket 6, and bracket 6 is reticulated structure, and filter membrane 5 is played a supporting role, and prevents that filter membrane 5 from coming off.The top of strainer tube is provided with pipe and covers 4, the bottom of strainer tube is provided with opening, opening part is provided with closing cap 7, the open outer side of strainer tube bottom is provided with outside screw, closing cap inner side is provided with internal thread, strainer tube bottom end opening is threaded with closing cap, the opening of strainer tube bottom also can with the equipment connections such as negative pressure lead device.
While gathering nucleic acid sample, air trapping pipe 1, strainer tube 2 are pegged graft, now do not use closing cap 7; After patient is deeply air-breathing, with air trapping duct occlusion patient oral area, patient's forced expiration, repeats 3 times, collects 5-10L patient and exhales; Then screw closing cap 7, remove air trapping pipe 1, strainer tube 2 is inserted in dissolution fluid collection tube; To the TE damping fluid or the deionized water that add 800 microlitres in strainer tube, lid upper tube cap, centrifugal 10 minutes of 1000g, makes liquid complete wetting filter membrane 5; Open pipe and cover 4, insert ultrasound probe, ultrasonic the gleanings stripping on filter membrane 5 through the short period of time; Screw off closing cap 7, lid upper tube cap 4, puts into a new dissolution fluid collection tube by chimney filter; 13000g high speed centrifugation 10 minutes, the dissolution fluid of collecting in dissolution fluid collection tube is determined nucleic acid sample, cryopreservation.

Claims (4)

1. for obtaining a collector for nucleic acid clinical samples, comprise air trapping pipe, strainer tube and dissolution fluid collection tube, it is characterized in that: air trapping pipe, strainer tube and dissolution fluid collection tube are from top to bottom pegged graft successively; In strainer tube, be provided with filter membrane, filter membrane is placed on netted bracket; The bottom of strainer tube is provided with opening, and opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid.
2. according to claim 1 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: the top of air trapping pipe, the bottom of strainer tube are all tapered.
3. according to claim 2 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: the opening of strainer tube bottom is threaded with closing cap.
4. according to claim 3 for obtaining the collector of nucleic acid clinical samples, it is characterized in that: filter membrane is made by glass fibre, silicon fibre, TCLP or silica gel.
CN201320106733.4U 2013-02-25 2013-02-25 Collector for obtaining nucleic acid clinical specimen Withdrawn - After Issue CN203403089U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201320106733.4U CN203403089U (en) 2013-02-25 2013-02-25 Collector for obtaining nucleic acid clinical specimen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201320106733.4U CN203403089U (en) 2013-02-25 2013-02-25 Collector for obtaining nucleic acid clinical specimen

Publications (1)

Publication Number Publication Date
CN203403089U true CN203403089U (en) 2014-01-22

Family

ID=49939027

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201320106733.4U Withdrawn - After Issue CN203403089U (en) 2013-02-25 2013-02-25 Collector for obtaining nucleic acid clinical specimen

Country Status (1)

Country Link
CN (1) CN203403089U (en)

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
AV01 Patent right actively abandoned

Granted publication date: 20140122

Effective date of abandoning: 20151125

C25 Abandonment of patent right or utility model to avoid double patenting