CN103571914B - Obtain acquisition method and the device of nucleic acid clinical samples - Google Patents
Obtain acquisition method and the device of nucleic acid clinical samples Download PDFInfo
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- CN103571914B CN103571914B CN201310068049.6A CN201310068049A CN103571914B CN 103571914 B CN103571914 B CN 103571914B CN 201310068049 A CN201310068049 A CN 201310068049A CN 103571914 B CN103571914 B CN 103571914B
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- filter membrane
- nucleic acid
- strainer tube
- clinical samples
- dissolution fluid
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Abstract
The present invention relates to a kind of acquisition method and the device that obtain nucleic acid clinical samples, the air of patient's exhalation is gathered by gathering device, by the air filtration that the filter membrane of gathering device is breathed out to patient, filter membrane is soaked in TE or deionized water, utilize ultrasonic the gleanings stripping on filter membrane, collect dissolution fluid, dissolution fluid is nucleic acid clinical samples.
Description
Technical field
The present invention relates to a kind of acquisition method and the device that obtain nucleic acid clinical samples.
Background technology
When medical science detects, often need the nucleic acid clinical samples gathering patient.Existing acquisition method mostly is and gathers peripheral blood in patients 3-5 milliliter or tissue sample 1-2mg, extracts nucleic acid by test kit, obtains nucleic acid clinical samples.But also do not have good method to collect from patient exhalation air and extract free nucleic acid sample.
Summary of the invention
Goal of the invention of the present invention is to provide a kind of acquisition method obtaining nucleic acid clinical samples, do not need blood sampling, easy and simple to handle, collection effect is good.
Based on same inventive concept, the present invention has two independently technical schemes:
1. one kind obtains the acquisition method of nucleic acid clinical samples, it is characterized in that: the air being gathered patient's exhalation by gathering device, by the air filtration that the filter membrane of gathering device is breathed out to patient, filter membrane is soaked in TE damping fluid or deionized water, utilize ultrasonic the gleanings stripping on filter membrane, collect dissolution fluid, dissolution fluid is nucleic acid clinical samples.
When being soaked in by filter membrane in TE damping fluid or deionized water, need 1000g centrifugal 10 minutes.
When collecting dissolution fluid, need 13000g centrifugal 10 minutes.
The air 5-10L of patient's exhalation is gathered by gathering device.
2. for realizing the gathering device of aforesaid method, it is characterized in that: the grafting successively from top to bottom of air trapping pipe, strainer tube and dissolution fluid collection tube, be provided with filter membrane in strainer tube, the bottom of strainer tube is provided with opening, opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid.
The top of air trapping pipe, the bottom of strainer tube are all tapered.
The opening of strainer tube bottom is threaded with closing cap.
The beneficial effect that the present invention has:
The nucleic acid material of microorganism or its release forms aerosol in atmosphere, and when aerosol is by filter membrane, macromole nucleic acid is wherein trapped within filter membrane.Infiltrate filter membrane with deionized water or TE damping fluid, by ultrasonic by the gleanings stripping on filter membrane, collect dissolution fluid and namely obtain nucleic acid clinical samples.The present invention does not need blood sampling, only needs patient expiration can realize the collection of nucleic acid clinical samples, simple to operate, quick, efficient, hurtless measure, and gathering device of the present invention not only can be applicable to the medical science such as tubercule bacillus detection and detects, and can also be widely used in environmental monitoring.
Accompanying drawing explanation
Fig. 1 is the structural representation of gathering device of the present invention;
Fig. 2 is the structural representation of gathering device strainer tube of the present invention.
Embodiment
As shown in Figure 1, gathering device comprises air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3, and the grafting successively from top to bottom of air trapping pipe 1, strainer tube 2 and dissolution fluid collection tube 3, leaves space between the bottom of strainer tube and the bottom of dissolution fluid collection tube.The gas that air trapping pipe 1 is breathed out for receiving patient, also can be connected with other air feed equipments.The top of air trapping pipe 1, the bottom of strainer tube 2 are all tapered.As shown in Figure 2, be provided with filter membrane 5 in strainer tube, filter membrane is made up of glass fibre, silicon fibre, TCLP or silica gel.Filter membrane 5 is placed on bracket 6, and bracket 6 is reticulated structure, plays a supporting role to filter membrane 5, prevents filter membrane 5 from coming off.The top of strainer tube is provided with pipe lid 4, the bottom of strainer tube is provided with opening, opening part is provided with closing cap 7, the open outer side of strainer tube bottom is provided with outside screw, internal thread is provided with inside closing cap, strainer tube bottom end opening is threaded with closing cap, the opening of strainer tube bottom also can with the equipment connections such as negative pressure lead device.
When gathering nucleic acid sample, by air trapping pipe 1, strainer tube 2 grafting, now do not use closing cap 7; After the dark air-breathing of patient, with air trapping duct occlusion patient oral area, patient's forced expiration, repeat 3 times, collect 5-10L patient expiration; Then screw closing cap 7, remove air trapping pipe 1, strainer tube 2 is inserted in dissolution fluid collection tube; In strainer tube, add TE damping fluid or the deionized water of 800 microlitres, lid upper tube cap, centrifugal 10 minutes of 1000g, makes liquid complete wetting filter membrane 5; Open pipe lid 4, insert ultrasound probe, ultrasonic the gleanings stripping on filter membrane 5 through the short period of time; Screw off closing cap 7, lid upper tube cap 4, chimney filter is put into a new dissolution fluid collection tube; 13000g high speed centrifugation 10 minutes, the dissolution fluid collected in dissolution fluid collection tube is determined nucleic acid sample, cryopreservation.
Claims (2)
1. one kind obtains the acquisition method of nucleic acid clinical samples, it is characterized in that: the air 5-10L being gathered patient's exhalation by gathering device, by the air filtration that the filter membrane of gathering device is breathed out to patient, filter membrane is soaked in TE damping fluid or deionized water, centrifugal 10 minutes of 1000g, utilizes ultrasonic the gleanings stripping on filter membrane, and 13000g collects dissolution fluid in centrifugal 10 minutes, dissolution fluid is nucleic acid clinical samples, and wherein filter membrane is made up of glass fibre, silicon fibre, TCLP or silica gel.
2. one kind for realizing the gathering device of method described in claim 1, it is characterized in that: the grafting successively from top to bottom of air trapping pipe, strainer tube and dissolution fluid collection tube, filter membrane is provided with in strainer tube, wherein filter membrane is made up of glass fibre, silicon fibre, TCLP or silica gel, the bottom of strainer tube is provided with opening, opening part is provided with closing cap, and the top of strainer tube is provided with pipe lid; The top of described air trapping pipe, the bottom of strainer tube are all tapered, and the opening of strainer tube bottom is threaded with closing cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310068049.6A CN103571914B (en) | 2013-02-25 | 2013-02-25 | Obtain acquisition method and the device of nucleic acid clinical samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310068049.6A CN103571914B (en) | 2013-02-25 | 2013-02-25 | Obtain acquisition method and the device of nucleic acid clinical samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103571914A CN103571914A (en) | 2014-02-12 |
| CN103571914B true CN103571914B (en) | 2015-11-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310068049.6A Active CN103571914B (en) | 2013-02-25 | 2013-02-25 | Obtain acquisition method and the device of nucleic acid clinical samples |
Country Status (1)
| Country | Link |
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| CN (1) | CN103571914B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2548122B (en) * | 2016-03-08 | 2018-09-12 | Univ Of Northumbria At Newcastle | Exhaled breath condensate collection device and a kit of parts therefor |
| CN109207340B (en) * | 2017-06-30 | 2022-08-12 | 开启基因股份有限公司 | Nucleic acid extraction assembly |
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2013
- 2013-02-25 CN CN201310068049.6A patent/CN103571914B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 呼吸道鼻病毒、SARS 病毒的Teflo 滤膜采集及RT-PCR 检测的初步研究;韩黎等;《军医进修学院学报》;20031231;第24卷(第4期);249-251 * |
| 采集呼气标本的时间对检测胃幽门螺杆菌感染检出率的影响;侯丽英;《山西医药杂志》;20060930;第35卷(第9期);836-837 * |
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| CN103571914A (en) | 2014-02-12 |
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