CN203385639U

CN203385639U - Improved biological chip micropore sensor

Info

Publication number: CN203385639U
Application number: CN201320517146.4U
Authority: CN
Inventors: 庄斌
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-08-23
Filing date: 2013-08-23
Publication date: 2014-01-08
Anticipated expiration: 2023-08-23

Abstract

The utility model discloses an improved biological chip micropore sensor. A micropore is formed among a waste liquor channel, an analysis channel and a reagent channel; a left transition channel and a right transition channel, which expand in a width direction, are formed at left and right sides of the micropore, respectively; the analysis channel and the reagent analysis form an acute angle, are communicated with each other near one side of the micropore, and are connected with the right transition channel. The improved biological chip micropore sensor is characterized in that the depth of the analysis channel, the reagent channel, the waste liquor channel, a sample tank, a reagent tank and a waste liquor tank is greater than that of the micropore; a left step part and a right step part, each of which consists of a plurality of steps and is gradually downward from the neighbor of the micropore, are formed on the bottom of the left transition channel and the bottom of the right transition channel respectively and extend to the bottom of the waste liquor channel as well as the bottoms of the analysis channel and the reagent channel respectively. Compared with the prior art, the sensitivity of the biological chip micropore sensor is improved, and the manufacturing difficulty is lowered.

Description

A kind of improved biochip micropore sensor

Technical field

The utility model relates to a kind of biochip micropore sensor of improved, and it belongs to the microflow controlled biochip class.

Background technology

Coulter principle is pointed out: be suspended in particle in electrolytic solution and during by the aperture pipe, replace the electrolytic solution of equal volume with electrolytic solution, cause two resistance between electrode generation transient change inside and outside the aperture pipe in the circuit of continuous current design, produce potential pulse.The size of pulse signal and number of times are directly proportional to size and the number of particle.The core micropore sensor of microflow controlled biochip utilizes Coulter principle to make.In order to detect cell or the particle by micropore, the sectional area size of micropore is all very little, there are some researches show that the micropore sectional area has effect preferably while being long-pending 2-20 times of cell or grain section, such as the diameter of spermatoblast 3 microns left and right, the sectional area of spermatoblast is about 9 microns squares, for this reason for the sectional area of the microflow controlled biochip micropore sensor of semen analysis generally between 50-300 micron square, dimensions commonly used has 5 * 10 microns, 10 * 50 microns and 30 * 100 microns squares etc., the depth dimensions that is wherein micropore than decimal fractions, the width dimensions that the plurality word is micropore, obviously the degree of depth of micropore is the restriction that is subject to cell and the long-pending size of grain section.On the other hand, what the biochip manufacture was mainly continued to use at present is the manufacturing technology of semi-conductor chip and laser data storage video disc, etch microchannel and further copy on macromolecular material on the silex glass material, such technique be it is generally acknowledged the micron order passage that is difficult to be formed with change in depth, for this reason, nearly all biochip design person and fabricator have selected single layer structure, the degree of depth that is all microchannels outside micropore is all consistent with the depth dimensions of micropore, with the size of micropore, compare, the size of other each microchannel only exists different on width.As previously mentioned, the degree of depth of micropore is subject to cell and grain section size restrictions, and in the single layer structure biochip, such restriction has also formed the restriction to other microchannel degree of depth in essence.Due to microchannel, particularly the breadth depth ratio of macromolecule microchannel has requirement, generally, between 2-10 times, is not more than at most 20 times, otherwise all can produce quality problems in manufacture and sealing-in, so single layer structure has also limited the width of each section microchannel of whole chip.Due to aforesaid reason, there is the problem of the following aspects in the biochip micropore sensor of prior art, and the one, each section microchannel flow is limited to the sectional area size of cell and particle, and the micro-fluidic chip that forms high flow capacity is comparatively difficult; The 2nd, in the biochip with the micropore sensor that adopts the impedance analysis technology, the resistance of the conducting solution in each microchannel distributes and is difficult to optimize, and directly has influence on the sensitivity of micropore sensor; The 3rd, the space that various functional modules are set in each microchannel has been subject to limitation, obviously for the micropore sensor, be distributed in the electrode of the micropore left and right sides the closer to micropore, the sensitivity of micropore sensor is higher, because reduced like this impact of the background resistance outside the micropore, but the difficulty that obviously electrode is manufactured the closer to micropore is just larger; The 4th, limited the selection of biochip packaging technology.In sum, obviously prior art has further improved necessity.

The utility model content

The purpose of this utility model is to provide a kind of improved biochip micropore sensor, the problem existed to overcome prior art.

A kind of improved biochip micropore sensor of the present utility model, comprise substrate and cover plate, form or be provided with micropore on described substrate, two sensing electrodes and microchannel, described microchannel comprises analysis channel, reagent passage and waste fluid channel, described micropore is located between waste fluid channel and analysis channel and reagent passage, and the left and right sides of described micropore forms respectively left transition passage and the right transition passage of dispersing on Width, described left transition passage connects with waste fluid channel, described waste fluid channel connects with waste liquid pool away from a side of micropore, described analysis channel and reagent passage are mutually in an acute angle and be interconnected in nearly described micropore one side, and connect with described right transition passage, an end away from micropore of described analysis channel and reagent passage connects with sample pool and reagent pond respectively, described two sensing electrodes lay respectively at the left and right sides of micropore, it is characterized in that described analysis channel, reagent passage, waste fluid channel, sample pool, the degree of depth of reagent pond and waste liquid pool is greater than the degree of depth of described micropore, the bottom of described left transition passage and right transition passage form respectively by some steps, formed and from nearly micropore downward left stage portion and right stage portion progressively, described left stage portion and right stage portion extend to respectively the bottom of waste fluid channel and analysis channel and reagent passage.

As preferably, a sensing electrode in described two sensing electrodes is arranged in waste liquid pool, and another sensing electrode is arranged in reagent pond or sample pool.

This improved biochip micropore sensor of the present utility model, prior art has been introduced the sandwich construction design philosophy of novelty relatively, on the basis of guaranteeing microcellular structure size conforms standard, deepened analysis channel, reagent passage, these microchannels of waste fluid channel, the degree of depth of the microchannel relative micropore that these have been deepened has formed hierarchical structure, simultaneously the utility model has been introduced respectively left stage portion and right stage portion structure as analysis channel in left transition passage and right transition passage, reagent passage, the waste fluid channel bottom is to the connection transition structure of micropore, adopt so such structure of structure one side to adopt the improved manufacturing technology of existing semi-conductor chip, it is the possibility that the etching technique of semiconductor manufacture has realization, simultaneously such structure is guaranteeing not damage the sectional area that has increased to greatest extent microchannel under the prerequisite of microchannel breadth depth ratio.

As shown in Figure 5, the equivalent electrical circuit of biochip micropore sensor is a resistance series circuit, wherein R1 is micropore resistance, R2 and R3 are microchannel electrolyte inside resistance, R4 and R5 are electrode resistance, obviously the resistance of micropore resistance R 1 changes, in the situation that sample is sperm, while not having sperm to pass through micropore, the resistance of micropore resistance R 1 is A1, while having sperm to pass through micropore, the resistance of micropore resistance R 1 is A2, simultaneously, obviously the size of A1 and the sectional area AS of micropore are inversely proportional to, the sectional area AS of the size of A2 and micropore is inversely proportional to the difference of sperm sectional area AC, while having a continuous current I to flow through in the equivalent electrical circuit of described biochip micropore sensor, the terminal voltage at equivalent electrical circuit two ends obviously equals the product of continuous current I and equivalent electrical circuit total resistance value, be that the terminal voltage V at equivalent electrical circuit two ends obviously equals I * (R1+R2+R3+R4+R5), while being defined in without sperm by micropore, the terminal voltage at equivalent electrical circuit two ends is V1, V1 equals I * (A1+R2+R3+R4+R5), when definition has sperm by micropore, the terminal voltage at equivalent electrical circuit two ends is V2, V2 equals I * (A2+R2+R3+R4+R5).The sensitivity definition of biochip micropore sensor is, the ratio of the difference of the total resistance value V2 of cell or particle equivalent electrical circuit during by micropore all-in resistance V1 of equivalent electrical circuit when there is no cell or particle by micropore all-in resistance V1 of equivalent electrical circuit when there is no cell or particle by micropore, i.e. (V2-V1)/V1, microchannel electrolyte inside resistance R 2 and R3 and electrode resistance R4 and R5 obviously can regard constant as, obviously this constant is less, the sensitivity of biochip micropore sensor is higher, the utility model is guaranteeing not damage the sectional area that has increased to greatest extent microchannel under the prerequisite of microchannel breadth depth ratio, increased analysis channel, reagent passage, the sectional area of waste fluid channel, thereby reduced the bath resistance in these microchannels, promoted the sensitivity of biochip micropore sensor for this reason.The raising of the sensitivity of biochip micropore sensor, also make two sensing electrodes of the present utility model can adopt a sensing electrode to be arranged in waste liquid pool, another sensing electrode is arranged on the technical scheme in reagent pond or sample pool, has so also reduced the manufacture difficulty of biochip micropore sensor.

In sum, obviously the purpose of this utility model is achieved.

The accompanying drawing explanation

Fig. 1 is a kind of improved biochip micropore sensor decomposition texture schematic diagram that the utility model preferred embodiment provides;

Fig. 2 is the board structure schematic diagram of a kind of improved biochip micropore sensor that provides of the utility model preferred embodiment;

Fig. 3 is that relative Fig. 2 of substrate of a kind of improved biochip micropore sensor that provides of the utility model preferred embodiment has converted the structural representation behind the visual angle;

Fig. 4 is the vertical view of the substrate of a kind of improved biochip micropore sensor that provides of the utility model preferred embodiment;

Fig. 5 is the equivalent circuit diagram of a kind of improved biochip micropore sensor that provides of the utility model preferred embodiment.

In each figure:

1 is substrate;

2 is cover plate;

3 is micropore;

4 is sensing electrode;

5 is analysis channel;

6 is reagent passage;

7 is waste fluid channel;

8 is left transition passage;

801 is left stage portion;

9 is right transition passage;

901 is right stage portion;

10 is waste liquid pool;

11 is the reagent pond;

12 is sample pool;

13 is sperm.

Embodiment

The utility model is described in further detail for a kind of improved biochip micropore sensor provided below with reference to the utility model preferred embodiment and accompanying drawing thereof.

A kind of improved biochip micropore sensor that the utility model preferred embodiment provides, as accompanying drawing 1, accompanying drawing 2, accompanying drawing 3, accompanying drawing 4, shown in accompanying drawing 5, comprise substrate 1 and cover plate 2, this is a kind of biochip micropore sensor that is used for the test analysis seminal fluid, this improved biochip micropore sensor of this preferred embodiment, form or be provided with micropore 3 on described substrate 1, two sensing electrodes 4 and microchannel, described microchannel comprises analysis channel 5, reagent passage 6 and waste fluid channel 7, described micropore 3 is located between waste fluid channel 7 and analysis channel 5 and reagent passage 6, and the left and right sides of described micropore 3 forms respectively left transition passage 8 and the right transition passage 9 of dispersing on Width, described left transition passage 8 connects with waste fluid channel 7, described waste fluid channel 7 connects with waste liquid pool 10 away from a side of micropore 3, described analysis channel 5 and reagent passage 6 are mutually in an acute angle and be interconnected in nearly described micropore 3 one sides, and connect with described right transition passage 9, an end away from micropore 3 of described analysis channel 5 and reagent passage 6 connects with sample pool 12 and reagent pond 11 respectively, described two sensing electrodes 4 lay respectively at the left and right sides of micropore 3, it is characterized in that described analysis channel 5, reagent passage 6, waste fluid channel 7, sample pool 12, the degree of depth of reagent pond 11 and waste liquid pool 10 is greater than the degree of depth of described micropore 3, the bottom of described left transition passage 8 and right transition passage 9 form respectively by three steps, formed and from nearly micropore 3 downward left stage portion 801 and right stage portion 901 progressively, described left stage portion 801 and right stage portion 901 extend to respectively the bottom of waste fluid channel 7 and analysis channel 5 and reagent passage 6.

In this preferred embodiment, a sensing electrode 4 in described two sensing electrodes 4 is arranged in waste liquid pool 10, and another sensing electrode 4 is arranged in reagent pond 11.

In sum, a kind of improved biochip micropore sensor of the present utility model, micropore is located between waste fluid channel and analysis channel and reagent passage, and the left and right sides of described micropore forms respectively left transition passage and the right transition passage of dispersing on Width, described analysis channel and reagent passage are mutually in an acute angle and be interconnected in nearly described micropore one side, and connect with described right transition passage, it is characterized in that described analysis channel, reagent passage, waste fluid channel, sample pool, the degree of depth of reagent pond and waste liquid pool is greater than the degree of depth of described micropore, the bottom of described left transition passage and right transition passage form respectively by some steps, formed and from nearly micropore downward left stage portion and right stage portion progressively, described left stage portion and right stage portion extend to respectively the bottom of waste fluid channel and analysis channel and reagent passage, relatively prior art the utility model promoted biochip micropore sensor sensitivity manufacture difficulty.

The above is only embodiment of the present utility model.But the utility model protection domain is not limited to this.Anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; the variation that can expect easily or replacement; within all should being encompassed in protection domain of the present utility model, therefore, protection domain of the present utility model should be as the criterion with the protection domain of claim.

Claims

1. an improved biochip micropore sensor, comprise substrate and cover plate, form or be provided with micropore on described substrate, two sensing electrodes and microchannel, described microchannel comprises analysis channel, reagent passage and waste fluid channel, described micropore is located between waste fluid channel and analysis channel and reagent passage, and the left and right sides of described micropore forms respectively left transition passage and the right transition passage of dispersing on Width, described left transition passage connects with waste fluid channel, described waste fluid channel connects with waste liquid pool away from a side of micropore, described analysis channel and reagent passage are mutually in an acute angle and be interconnected in nearly described micropore one side, and connect with described right transition passage, an end away from micropore of described analysis channel and reagent passage connects with sample pool and reagent pond respectively, described two sensing electrodes lay respectively at the left and right sides of micropore, it is characterized in that described analysis channel, reagent passage, waste fluid channel, sample pool, the degree of depth of reagent pond and waste liquid pool is greater than the degree of depth of described micropore, the bottom of described left transition passage and right transition passage form respectively by some steps, formed and from nearly micropore downward left stage portion and right stage portion progressively, described left stage portion and right stage portion extend to respectively the bottom of waste fluid channel and analysis channel and reagent passage.

2. a kind of improved biochip micropore sensor according to claim 1, is characterized in that a sensing electrode in described two sensing electrodes is arranged in waste liquid pool, and another sensing electrode is arranged in reagent pond or sample pool.