CN106198363B - Flow cytometry detection chip - Google Patents
Flow cytometry detection chip Download PDFInfo
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- CN106198363B CN106198363B CN201610822592.4A CN201610822592A CN106198363B CN 106198363 B CN106198363 B CN 106198363B CN 201610822592 A CN201610822592 A CN 201610822592A CN 106198363 B CN106198363 B CN 106198363B
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- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
Abstract
The invention discloses a flow cytometry detection chip, which is sequentially compounded with a first protection film, a first sealing film, a microfluid chip and a second sealing film which can be peeled from top to bottom; the first sealing film is provided with at least four electrode holes, a sample adding hole and an air outlet hole; the microfluidic chip is provided with a liquid storage pool, a sample adding pool, a detection channel, a waste liquid pool and at least two detection holes positioned at two ends of the detection channel, wherein the liquid storage pool, the sample adding pool, the detection channel and the waste liquid pool are communicated with each other from top to bottom according to the using direction of the chip; the four electrode holes are arranged at positions corresponding to the inlets of the liquid storage tank, the two detection holes and the waste liquid tank respectively; the setting position of the sample adding hole corresponds to the sample adding pool; the setting position of the air outlet corresponds to the waste liquid pool. The invention provides the power for the liquid flow by utilizing the gravity principle, so the invention does not need to be additionally driven, has small chip volume and low cost, uses miniaturized equipment and saves energy, and simultaneously sets the electrode holes for sampling detection to ensure the precision of the detection result.
Description
Technical Field
The invention relates to a flow cytometry detection chip.
Background
The flow type microparticle detection is a technology for quantitatively analyzing and sorting microparticle particles in a liquid one by one, can measure the size, shape, concentration, activity and the like of the microparticles, and has wide application in the subjects of hematology, immunology, molecular biology and the like. The coulter principle adopted in the detection is as follows: when the particles suspended in the electrolyte pass through the small holes along with the electrolyte, the particles replace the electrolyte with the same volume, the resistance between the inner electrode and the outer electrode of the small holes is instantaneously changed in a constant current designed circuit, potential pulses are generated, and the size and the frequency of pulse signals are in direct proportion to the size and the number of the particles. Sample focusing is a key technology of flow type particle detection, chips for focusing sample liquid under the action of external force are adopted in the current detection, detection electrodes are integrated in the chips, the size is large, negative pressure is pumped during use to provide power, buffer solution needs to be added into the chips during use, and how much buffer solution is added can influence the use of the chips.
Disclosure of Invention
The invention aims to provide a flow cytometry detection chip which does not need external force to drive, is small and convenient.
The technical scheme for realizing the aim of the invention is that the flow cytometry detection chip is compounded with a first protection film, a first sealing film, a microfluid chip and a second sealing film which can be stripped from top to bottom in sequence; the first sealing film is provided with at least four electrode holes, a sample adding hole and an air outlet hole; the microfluidic chip is provided with a liquid storage pool, a sample adding pool, a detection channel, a waste liquid pool and at least two detection holes positioned at two ends of the detection channel, wherein the liquid storage pool, the sample adding pool, the detection channel and the waste liquid pool are communicated with each other from top to bottom according to the using direction of the chip; the four electrode holes are arranged at positions corresponding to the inlets of the liquid storage tank, the two detection holes and the waste liquid tank respectively; the setting position of the sample adding hole corresponds to the sample adding pool; the setting position of the air outlet corresponds to the waste liquid pool.
Each electrode hole is at least one dashed line which penetrates through the first sealing film; and a buffer solution is preset in the liquid storage tank.
Six electrode holes are formed; the number of the detection holes is four, and two detection holes are arranged at two ends of the detection channel respectively; and the positions of the first sealing film corresponding to the four detection holes are respectively provided with an electrode hole.
And a balance hole is arranged between the electrode hole with the lowest position and the air outlet hole on the first sealing film.
The air outlet is a non-circular hole; or the air outlet and the balance hole are both non-circular holes.
The depths of the liquid storage pool, the sample adding pool, the detection channel and the waste liquid pool on the microfluid chip are different.
The first sealing film is an opaque film.
And an opaque second protective film is compounded at the bottom of the second sealing film.
The first protective film and the second sealing film are both transparent films.
And the electrode hole with the highest position on the first sealing film is simultaneously used as an air inlet hole.
After the technical scheme is adopted, the invention has the positive effects that: (1) the invention provides the power for the liquid flow by utilizing the gravity principle, so the invention does not need to be additionally driven, has small chip volume and low cost, uses miniaturized equipment and saves energy, and simultaneously sets the electrode holes for sampling detection to ensure the precision of the detection result.
(2) The electrode hole of the invention adopts a perforated but not hollowed-out hole, so that the electrode is convenient to arrange when in use, and simultaneously, the liquid can be blocked, the buffer solution is prevented from being spilled, and the buffer solution can be preset in the chip by the structure, thereby being convenient for detection.
(3) The balance hole is positioned above the air outlet and is used for supplementing the air outlet, so that even if air bubbles are generated at the air outlet during transportation or use, liquid can still smoothly circulate.
(4) In the prior art, circular air outlets are adopted, but liquid in the circular air outlets easily flows in the circular air outlets, and the environment of the conductor liquid is disordered.
(5) The first protection film can form a closed environment for the chip, so that total dislocation of liquid in transportation and storage is avoided, the environment is stable relative to the liquid level, no liquid flows into a waste liquid pool before use, and the liquid flows downwards under the action of gravity after being torn; meanwhile, the first protection film can also protect liquid in the chip to be clean.
(6) The first sealing film and the second sealing film adopt opaque films, so that the liquid can be prevented from being deteriorated by light.
(7) The second protective film is a transparent film, so that quality inspection is facilitated.
(8) The depth of each pool of the chip is different, which is beneficial to providing different driving forces when in use.
Drawings
In order that the present disclosure may be more readily and clearly understood, reference is now made to the following detailed description of the present disclosure taken in conjunction with the accompanying drawings, in which
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural view of the first sealing film of the present invention.
FIG. 3 is a schematic structural diagram of a microfluidic chip according to the present invention.
The reference numbers in the drawings are as follows:
the micro-fluidic chip comprises a first protection film 1, an electrode hole 21, a first electrode hole 21-1, a second electrode hole 21-2, a third electrode hole 21-3, a fourth electrode hole 21-4, a fifth electrode hole 21-5, a sixth electrode hole 21-6, a sample adding hole 22, an air outlet hole 23, a balance hole 24, a first sealing film 2, a micro-fluidic chip 3, a liquid storage tank 31, a sample adding tank 32, a waste liquid tank 33, a detection hole 34, a second sealing film 4 and a second protection film 5.
Detailed Description
(example 1)
Referring to fig. 1, 2 and 3, a flow cytometry detection chip of the present embodiment is composed of a first peelable protective film 1, a first sealing film 2, a microfluidic chip 3, a second sealing film 4 and a second protective film 5 sequentially from top to bottom; six electrode holes 21, a sample adding hole 22 and an air outlet hole 23 are formed in the first sealing film 2; the microfluid chip 3 is provided with a liquid storage tank 31, a sample adding tank 32, a detection channel and a waste liquid tank 33 which are communicated with each other from top to bottom according to the using direction of the chip, and two pairs of four detection holes 34 which are positioned at two ends of the detection channel; the setting positions of the six electrode holes 21 are respectively as follows: a first electrode hole 21-1 corresponding to the liquid storage tank 31, a second electrode hole 21-2 corresponding to the four detection holes 34, a third electrode hole 21-3, a fifth electrode hole 21-5, a sixth electrode hole 21-6, and a fourth electrode hole 21-4 corresponding to the inlet of the waste liquid tank 33; of course, only four electrode holes 21 may be provided, and only one electrode hole may be provided at each of both ends of the detection channel, so that the accuracy of the detection data may be slightly poor. The setting position of the sample adding hole 22 corresponds to the sample adding pool 32; the position of the air outlet corresponds to the waste liquid pool 33. Each electrode hole 21 is at least one short-dashed line drawn through the first sealing film 2, and may be formed in a shape of a Chinese character 'mi' as shown in fig. 2 or other shapes; the buffer solution is preset in the liquid storage tank 31. Balance holes 24 are provided between the lowest-positioned electrode hole 21 and the air outlet hole 23 on the first sealing film 2. The air outlet 23 is a non-circular hole; or both the outlet holes 23 and the balance holes 24 are non-circular holes. The depths of the reservoir 31, the sample addition reservoir 32, the detection channel and the waste liquid reservoir 33 on the microfluidic chip 3 are different. The first sealing film 2 is an opaque film. And an opaque second protective film 5 is compounded at the bottom of the second sealing film 4. The first protection film 1 and the second sealing film 4 are both transparent films. The first electrode hole 21-1 positioned highest on the first sealing film 2 serves as an air intake hole at the same time.
During the use, tear off first protection film 1 can, insert each electrode from electrode hole 21, owing to have the line of drawing through, therefore the electrode inserts very conveniently, adds the tissue liquid that will wait to detect and can connect detecting instrument to detect from the application of sample hole. When in production, the second protective film 5 is not attached, and the second sealing film 4 is transparent, so that quality inspection can be conveniently carried out, the quality inspection passes, and then the second protective film 5 is attached.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A flow cytometry detection chip, comprising: the micro-fluid chip is characterized in that a first protection film (1), a first sealing film (2), a micro-fluid chip (3) and a second sealing film (4) which can be stripped are compounded from top to bottom in sequence; at least four electrode holes (21), a sample adding hole (22) and an air outlet hole (23) are formed in the first sealing film (2); the microfluid chip (3) is provided with a liquid storage pool (31), a sample adding pool (32), a detection channel, a waste liquid pool (33) and at least two detection holes (34) which are communicated from top to bottom according to the using direction of the chip; the four electrode holes (21) are respectively arranged at the positions corresponding to the inlets of the liquid storage tank (31), the two detection holes (34) and the waste liquid tank (33); the arrangement position of the sample adding hole (22) corresponds to the sample adding pool (32); the arrangement position of the air outlet hole corresponds to the waste liquid pool (33); each electrode hole (21) is at least one dashed line which penetrates through the first sealing film (2); a buffer solution is preset in the liquid storage tank (31); the air outlet (23) is a non-circular hole; a balance hole (24) is arranged between the electrode hole (21) with the lowest position and the air outlet hole (23) on the first sealing film (2); the balance holes (24) are all non-circular holes; the electrode hole (21) with the highest position on the first sealing film (2) is simultaneously used as an air inlet hole.
2. A flow cytometry detection chip as described in claim 1 wherein: six electrode holes (21) are arranged; the number of the detection holes (34) is four, and two ends of the detection channel are respectively provided with two; and the positions of the first sealing film (2) corresponding to the four detection holes (34) are respectively provided with an electrode hole (21).
3. A flow cytometry detection chip as described in claim 1 or 2 wherein: the depths of the liquid storage pool (31), the sample adding pool (32), the detection channel and the waste liquid pool (33) on the microfluidic chip (3) are different.
4. A flow cytometry detection chip as described in claim 3 wherein: the first sealing film (2) is an opaque film.
5. A flow cytometry detection chip as described in claim 4 wherein: and an opaque second protective film (5) is compounded at the bottom of the second sealing film (4).
6. A flow cytometry detection chip as described in claim 5 wherein: the first protection film (1) and the second sealing film (4) are both transparent films.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610822592.4A CN106198363B (en) | 2016-09-13 | 2016-09-13 | Flow cytometry detection chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610822592.4A CN106198363B (en) | 2016-09-13 | 2016-09-13 | Flow cytometry detection chip |
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| CN106198363A CN106198363A (en) | 2016-12-07 |
| CN106198363B true CN106198363B (en) | 2020-08-07 |
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| CN107213930B (en) * | 2017-07-27 | 2022-12-20 | 深圳中科芯海智能科技有限公司 | Microfluidic chip for particle analysis and particle analysis method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN204556464U (en) * | 2015-04-15 | 2015-08-12 | 镇江吉蕊生物科技有限公司 | A kind of FCM analysis chip |
| CN105510191A (en) * | 2015-11-19 | 2016-04-20 | 江苏卓微生物科技有限公司 | Flow-type particle detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2261650A3 (en) * | 2004-09-15 | 2011-07-06 | IntegenX Inc. | Microfluidic devices |
| CN103977848B (en) * | 2007-04-06 | 2016-08-24 | 加利福尼亚技术学院 | Microfluidic device |
| WO2009105711A1 (en) * | 2008-02-21 | 2009-08-27 | Decision Biomarkers, Inc. | Assays based on liquid flow over arrays |
| CN115266543A (en) * | 2013-10-30 | 2022-11-01 | Abs全球公司 | Apparatus and associated method for identifying an object |
| CN104031823B (en) * | 2014-06-27 | 2016-08-24 | 江苏卓微生物科技有限公司 | cell detection chip and adapter thereof |
| CN104073427B (en) * | 2014-06-27 | 2016-03-30 | 江苏卓微生物科技有限公司 | Cell detection adaptor chip |
| CN203960191U (en) * | 2014-06-27 | 2014-11-26 | 江苏卓微生物科技有限公司 | cell detection chip and adapter thereof |
| CN204405503U (en) * | 2014-12-19 | 2015-06-17 | 江苏卓微生物科技有限公司 | A kind of portable quick particle detection |
| CN204731136U (en) * | 2015-06-29 | 2015-10-28 | 江苏卓微生物科技有限公司 | A kind of flow cytometry detection device |
| CN206074412U (en) * | 2016-09-13 | 2017-04-05 | 江苏卓微生物科技有限公司 | A kind of FCM analysis chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204556464U (en) * | 2015-04-15 | 2015-08-12 | 镇江吉蕊生物科技有限公司 | A kind of FCM analysis chip |
| CN105510191A (en) * | 2015-11-19 | 2016-04-20 | 江苏卓微生物科技有限公司 | Flow-type particle detection method |
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Denomination of invention: A Flow Cytometric Detection Chip Effective date of registration: 20230811 Granted publication date: 20200807 Pledgee: China Construction Bank Corporation Changzhou Xinbei sub branch Pledgor: JIANGSU JIMBIO TECHNOLOGY Co.,Ltd. Registration number: Y2023980051702 |
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