CN203379140U

CN203379140U - DNA (Deoxyribonucleic Acid) sample collector

Info

Publication number: CN203379140U
Application number: CN201320236034.1U
Authority: CN
Inventors: 渠新风
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-04-28
Filing date: 2013-04-28
Publication date: 2014-01-08
Anticipated expiration: 2023-04-28

Abstract

The utility model discloses a DNA (Deoxyribonucleic Acid) sample collector based on a solid sample storage medium. The DNA sample collector comprises a sampling brush, a sample card and a sample card sealing bag, wherein the sampling brush comprises a platform-shaped brush head and a brush handle which is made of a plastic tubulose material; the brush head consists of a metal fixed rod and nylon hairs arranged on the metal fixed rod; brush head protection glue which is made of non-toxic plastic is arranged at the end part of the metal fixed rod; the sample card comprises a solid sample storage medium fixed frame, the rectangular, circular or elliptic solid sample storage medium which is arranged in the solid sample storage medium fixed frame and a circular needle hole thread which is preset on a solid sample processing medium; the sampling card sealing bag comprises an envelope type sealing cover and a code bar label which is connected to the envelop type sealing cover. The DNA sample collector represents the maturity and practicability of a vagina self-sampling technique, and thoroughly changes the traditional mode that the cervical cancer screening is only carried out in the hospital; large-area cervical cancer screening becomes possible in short time, and the long-distance transportation problem is also solved.

Description

The DNA sample harvester

Technical field

This utility model relates to medical instruments field, particularly a kind of DNA sample harvester.

Background technology

Cervical cancers in women examination need at first in women's intravaginal, to obtain the detachment of cervix cytological samples, by detecting " the human papilloma sample virus (HPV) " in exfoliative cyte, examine out positive patient and give early diagnosis and processing.Obtain exfoliative cytology sample in vagina and need " DNA sample harvester ".

All take the liquid sample storage medium as basis for the DNA sample harvester of cervical cancer examination at present, and the Sample preservation liquid that the container that it is not waited by a 5-25 milliliter and main chemical compositions are ethanol forms, and some is furnished with sampling brush, and some does not have.The operation principle of this class sampler is that the medical worker obtains the exfoliative cyte sample with sampling brush from women's cervix uteri position, then will in Sample preservation liquid, soak with the sampling brush of sample.Finally, use the Sample preservation liquid in container, detected by extracting viral DNA.

The sampling brush brush that this class sampler adopts is tapered, and handle is shorter, and a break is arranged, after facilitating the doctor to sample, directly brushhead portion is fractureed and put into Sample preservation liquid.But for layman's oneself sampling, during due to sampling, can not use vaginal dilator and can't see sampling point, the cervix uteri place that shorter handle is difficult for sampling brush is delivered to the vagina top obtains the sample of suitable position; The layman, make, the break on brush holder is easy to make the disconnected intravaginal of losing in the women of brush, causes danger.

This class DNA sample harvester adopts liquid sample to preserve liquid, and when the layman uses, liquid is easy to spill, pollute, so this class DNA sample harvester can only be by the professional and technical personnel in the use of professional medical internal institution, and unsuitable long-distance transportation.Or need special protector to transport.Another shortcoming of liquid storage liquid is that volume is large, and weight is large, allows to transportation, and cost of transportation is also very high.

All employing liquid samples are preserved the sample of liquid storage, storage be all complete cell, need at first carry out DNA extraction in laboratory, then can be detected.

Along with the birth that certainly samples technology, the sampling work of cervical cancer examination can complete at home by being examined people oneself, then is addressed to inspection center and completes detection and get final product.So one of market demand can effectively be stored DNA sample, be convenient to non-medical skill librarian use, and can long-distance transportation can not cause because of spilling the DNA sample harvester of the mutual pollution of sample, take solid-state sample storage medium as basic DNA sample harvester.

The utility model content

The technical problems to be solved in the utility model is to provide a kind of common woman oneself that facilitates and completes the designed DNA sample harvester of the needed vagina sample program of cervical cancer examination.

To achieve these goals, the technical solution of the utility model is as follows: a kind of DNA sample harvester, comprise sampling brush, sample card and sample card sealing bag, described sampling brush comprises the brush of bench-type and the brush holder that the plastic tube-shape material is made, brush is by the metal fixed bar, and the nylon hair be located on the metal fixed bar forms, and be provided with the brush protection glue that nontoxic plastic cement makes in the end of metal fixed bar, described sample card comprises solid-state sample storage medium fixed mount, and be located at rectangle on solid-state sample storage medium fixed mount, circular or oval solid-state sample storage medium, described sample card sealing bag comprises the envelope capping, and the bar code label of connection envelope capping.

Further, described solid-state sample storage medium fixed mount material is papery hard card or plastics.

Further, the integral thickness of described sample card is 1-1.5mm.

Further, the solid-state sample storage medium on described sample card is preset with the circular pin hole line of diameter 6-12 millimeter.

Adopt the DNA sample harvester of technique scheme, because described sampling brush comprises the brush of bench-type and the brush holder that the plastic tube-shape material is made, for obtain the exfoliative cyte sample in vagina, its operation principle is to utilize the rotation of brush fibril that vaginal exfoliated is collected on the brush fibril, because described sample card comprises solid-state sample storage medium fixed mount, and be located at solid-state sample storage medium rectangular-shaped on solid-state sample storage medium fixed mount, described solid-state sample storage medium fixed mount material is papery hard card or plastics, to sample brush after women sampling smears and the sample obtained can be coated on to sampling and block on the solid-state sample storage medium of sample card, if the solid-state sample storage medium of sample card central authorities contains specific chemical substance, can adsorb the exfoliative cyte in sample, and adsorption moisture, reach the purpose of long preservation sample, the chemical substance of adhering on solid-state sample storage medium can be directly by lysis, discharge viral DNA, like this, in laboratory, as long as with warm water by the DNA elution out, direct-detection just can, removed the leaching process of DNA from, owing to having adopted solid-state sample storage medium, sample can not spill, and can not cause the mutual pollution between sample, and the sample card volume is little, lightweight, is convenient to the transportation of long distance, and can transports by logistics company, because described sample card sealing bag comprises the envelope capping, and connect the bar code label of envelope capping, the sample card sealing bag is that its effect is mainly to comprise and isolate every sampling card when the collective conveyance sample for sealing the sample card that is coated with upper sample up for safekeeping, whole DNA sample harvester is packaged in the outer packing plastic of a sealing, is printed with the sampling guide in packaging bag.

The accompanying drawing explanation

In order to be illustrated more clearly in this utility model embodiment or technical scheme of the prior art, below will the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.

Fig. 1 is sampling brush structural representation in the attached DNA sample harvester of this utility model;

Fig. 2 is sample card structural representation in the attached DNA sample harvester of this utility model;

Fig. 3 is sample card sealing bag structural representation in the attached DNA sample harvester of this utility model.

The specific embodiment

Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail, thereby so that advantage of the present utility model and feature can be easier to be it will be appreciated by those skilled in the art that, protection domain of the present utility model is made to more explicit defining.

As Fig. 1, shown in Fig. 2 and Fig. 3, a kind of DNA sample harvester, comprise sampling brush, sample card and sample card sealing bag, described sampling brush comprises the brush 1 of bench-type and the brush holder 2 that the plastic tube-shape material is made, brush 1 is by metal fixed bar 3, and the nylon hair 4 be located on metal fixed bar 3 forms, and be provided with the brush protection glue 5 that nontoxic plastic cement makes in the end of metal fixed bar 3, described sample card comprises solid-state sample storage medium fixed mount 6, and be located at rectangle on fixed mount 6, circular or oval-shaped solid-state sample storage medium 7, described sample card sealing bag comprises envelope capping 8, and the bar code label 9 of connection envelope capping 8.

Wherein, described solid-state sample storage medium fixed mount 6 materials are papery hard card or plastics.

The integral thickness of described sample card is 1-1.5mm.

Solid-state sample storage medium on described sample card is preset with the circular pin hole line 10 of diameter 6-12 millimeter.

Sampling brush as shown in Figure 1, the PA66 nylon fibril that the brush 1 of sampling brush is 0.10mm by abundant diameter forms, and perpendicular to brush holder 2 long axis directions, is 360.Arrange.Its working method is, the head end of sampling brush is deep in vagina, and through vaginal vault, then by twisted brush holder 2, drives brush 1 left rotation and right rotation, and vagina (cervix uteri) exfoliative cyte are linked with on brush 1 fibril of sampling brush.There is no break on sampling brush, adopted comparatively tough and tensile PE material, using larger-diameter tubulose as brush holder 2, avoided the risk of this section of possibility in the sampling process.

Sample card as shown in Figure 2, after the women completes sampling, slowly shift out vagina by sampling brush, and then by the brush of sampling brush 1, the sample dispensing area on the Sample preservation card is smeared repeatedly.The solid-state sample storage medium of sample applying area is that absorption paper has specific chemical substance, after the sample coating, color there will be change, with indication sample particular location and the zone of smearing, be convenient to place, lab assistant recognition sample place, obtain correct sample for detection of.Sample applying area filter paper on sample card also contains the special chemical material, can the cracking vaginal exfoliated, obtain viral DNA.Laboratory technicians only needs on the treating the preponderant disease instead of the secondary disease scale to cut a fritter and contains the filter paper with sample, boils certain hour in boiling water, gets final product direct-detection, without the step of carrying out complicated extraction DNA.In addition, can also preset diameters on the solid-state sample storage medium of sample applying area the circular pin hole line that is the 6-12 millimeter so that lab assistant obtains sample cheaply, test.Solid-state sample storage medium be basic sample card when Clinical Laboratory, need the sub-fraction of the solid-state sample storage medium of laboratory technicians clip, directly be put into processing and the check of in vitro carrying out sample.The mode of the solid-state sample storage medium of clip has two kinds, automatic punch clip or manual clip.Need to not preset the pin hole line on the solid-state sample process medium of sample card by automatic punch clip sample, automatic punch can complete the clip of sample voluntarily.During by manual clip sample, operator must tool using or utensil see district's sample, each clip all needs instrument is carried out disinfection, otherwise will be in the cross-contamination that becomes sample.In addition, complicated manual operation has also increased the probability of operator's contaminated samples greatly.Therefore, operator must prepare sufficient cutfit, and cause artificial pollution while needing careful operation just can avoid the clip sample, very inconvenient in actual the use.For the ease of the laboratory that do not have ability to buy automatic punch can use easily this DNA sample harvester (in sample card obtain sample, our technical scheme is: the pin hole line 1-that to have preset diameter on the solid-state sample storage medium of sample card be the 6-12 millimeter is several.The pin hole line has been arranged, laboratory operation personnel can use disposable disinfecting cotton swab rod or toothpick to push any one pin hole line zone, can be easily sub-fraction on the solid-state sample storage medium of clip obtain required sample and test, thereby avoided reusing the probability that sample clip instrument and manual operation cause sample contamination, guarantee on sample card to still have enough samples, for storage, in order to check use again in the future.Each solid-state sample storage district is preset with 1-N the circular pin hole do not waited, leaving certain area between circular pin hole line separates each pin hole line, to guarantee that solid-state sample storage district still keeps complete structure and shape after the clip sample, be convenient to need a plurality of sample chips of clip to be detected simultaneously.

Sample card sealing bag as shown in Figure 3, when the sample shellfish be coated on sampling card upper after, allow its natural air drying 5-10 minute, it can be sealed up for safekeeping in the sample card storage bag.Preservation, with sealing strip, is easy to adhesive seal.Sample card very light in weight after sealing, volume is very little, has evaded the restriction of logistic industry to liquid transport, can realize with lower cost the transportation of long distance.

The main innovate point of this utility model DNA sample harvester is:

1) lengthened length and the width of sampling brush brush, increased the diameter of bristle, and made brush become bench-type.Such design makes brush more easily obtain the vaginal exfoliated sample.

2) brush holder of sampling brush has adopted tubular material, has removed break, but and increase length, make it women that is more convenient for be more enough under stance sampling brush fully is deep into to vaginal vault, the oneself obtains vaginal sample.

3) the sampling card has adopted absorption paper as solid-state sample storage medium, and the layman that is more convenient for operates use.

4) use of FTA Elute Paper can directly be understood and DNA, realizes the long preservation of sample, and can indicate whether to exist sample and sample domain of the existence by the transformation of color.

The design of 5 sampling card profiles takes into account the needs that user grips card, in left side, Sample preservation district, a gripping area is arranged, and has effectively prevented from polluting the finger of user.The design of sampling card overall dimensions can meet the requirement of professional automatic punch.

6) on sampling Herba Carlinae biebersteinii envelope except can preserving salable sample, also designed two subsidiary title pages, one for pasting experimenter's special-purpose bar code, is convenient to the experimenter and completes after sampling the preservation of tearing; Another is convenient to laboratory when detecting sample for registering experimenter's personal information, cuts this title page, completes the note shorthand typing of experimenter's personal information.

7) be printed with detailed sampling guide in the outer package of DNA sample harvester, be convenient to user and consult sampling.

The birth of the DNA sample harvester due to this based on solid-state sample storage medium, make the examination of cervical cancer to carry out in the community activity mode, like this, most community female is registered to queue up and both can be realized cervical cancer examination timely without rushing towards hospital.The long-distance transportation problem that this technology also solves, also can allow the women enjoy high-quality cervical cancer examination detection even this makes in the remote countryside area of medical resource scarcity.The exploitation of this technology has thoroughly changed the present mode that the cervical cancer examination is only carried out in hospital, makes large-area cervical cancer examination in the short time become possibility.

Above demonstration and described ultimate principle of the present utility model, principal character and advantage of the present utility model.The technical staff of the industry should understand; this utility model is not restricted to the described embodiments; that in above-described embodiment and description, describes just illustrates principle of the present utility model; under the prerequisite that does not break away from this utility model spirit and scope, this utility model also has various changes and modifications, and these changes and improvements all fall in claimed this utility model scope.The claimed scope of this utility model is defined by appending claims and equivalent thereof.

Claims

1. a DNA sample harvester, comprise sampling brush, sample card and sample card sealing bag, it is characterized in that: described sampling brush comprises the brush of bench-type and the brush holder that the plastic tube-shape material is made, brush is by the metal fixed bar, and the nylon hair be located on the metal fixed bar forms, and be provided with the brush protection glue that nontoxic plastic cement makes in the end of metal fixed bar, described sample card is basic sample card for adopting solid-state sample storage medium, comprise solid-state sample storage medium fixed mount, and be located at rectangle on solid-state sample storage medium fixed mount, circular or oval-shaped solid-state sample storage medium, described sample card sealing bag comprises the envelope capping, and the bar code label of connection envelope capping.

2. DNA sample harvester as claimed in claim 1, it is characterized in that: described solid-state sample storage medium fixed mount material is papery hard card or plastics.

3. DNA sample harvester as claimed in claim 1, it is characterized in that: the integral thickness of described sample card is 1-1.5mm.

4. DNA sample harvester as claimed in claim 1, is characterized in that: the circular pin hole line that on the solid-state sample storage medium of described sample card, preset diameters is the 6-12 millimeter.