CN203376329U - Test strip for testing high-sensitivity sulfuric acid oestrone - Google Patents
Test strip for testing high-sensitivity sulfuric acid oestrone Download PDFInfo
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- CN203376329U CN203376329U CN201320541335.5U CN201320541335U CN203376329U CN 203376329 U CN203376329 U CN 203376329U CN 201320541335 U CN201320541335 U CN 201320541335U CN 203376329 U CN203376329 U CN 203376329U
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Abstract
A test strip for testing high-sensitivity sulfuric acid oestrone is composed of a microvoid reagent (8) and a test strip body (9). The test strip is characterized in that the microvoid reagent (8) comprises a back plate (1), a protective film sticker (5-1), a sample pad (2), a cellulose membrane (3), a water absorption pad (4) and a protective film sticker (5-2) are respectively pasted on the back plate from left to right, wherein the protective film sticker (5-1), the sample pad (2), the cellulose membrane (3), the water absorption pad (4) and the protective film sticker (5-2) are respectively provided with a mark, a control line C line (7) and a test line T line (6) are arranged on the cellulose membrane (3) respectively, wherein the C line (7) marks a second antibody of goat antimouse, the T line (6) marks a sulfuric acid oestrone compound of bovine serum albumin, and a sulfuric acid oestrone monoclonal antibody layer (8-1) is arranged in the microvoid reagent (8). The test strip is high in accuracy, simple in operation and suitable for being massively popularized and applied, and the fertilization conditions of pigs can be found in early periods.
Description
Technical field
The present invention relates to field of biological detection, be specially a kind of high sensitivity OES test strip.
Background technology
Along with the development of intensive industrialized piggery, the sow diagnosis of early gestation is more and more obvious to the effect that improves sow breeding potential and pig farm economic benefit.Sow gestation is made in time and accurately to judgement, can reduce nonpregnantly, and nonpregnant sow is recruited, thereby reduce the feed cost increased because of invalid raising.Traditional sow diagnosis of early gestation method mainly contains the methods such as Disease Diagnosis of Veterinary observation, Urinary hormones detection method, hormone response observation and Ultrasonic Diagnosis.Wherein the Disease Diagnosis of Veterinary observation mainly depends on the situation of oestrusing that animal doctor and experienced producers observe sow after breeding or artificial insemination, determine whether it becomes pregnant, personnel are had relatively high expectations, general grass-roots work personnel are difficult to grasp, and return not necessarily gestation of feelings sow, can be subject to the factor impacts such as the disorder of factors such as hormone secretion, uterine disease; The Urinary hormones detection method is mainly that its accuracy is high by the OES content in radio-immunity or euzymelinked immunosorbent assay (ELISA) working sample, but complex operation, also need to configure corresponding detecting instrument, cost is higher, and general plant is difficult to bear, also can't accomplish Site Detection in addition, on-the-spot judgement; Supersonic testing method depends on ultrasonic detecting equipment, its testing result is accurate, not only can judge pregnancy, can also detect the number of determining fetus, can also judge sex of foetus when measure late period, but the much tens thousand of units of its cost, disposable input cost is higher, and small-sized manufacturing enterprise or individual raiser's majority are reluctant to purchase.In conjunction with the actual conditions of the current pig industry of China, in order to solve the problem of sow diagnosis of early gestation, be necessary to develop a kind of low cost, detect device for fast detecting accurate and the energy Site Detection, meet the detection demand of grass-roots unit.
OES (Estrone sulfate) is a kind of steroid hormone oestrogen-like hormone, has the luxuriant and rich with fragrance parent nucleus of cyclopentane many hydrogen.Pregnant horse, all have this parahormone in the bodies such as farrowing sow.Farrowing sow gestation starts to produce estrogen on the 12nd day, is mainly oestrone, and the OES that is bonding state in the internal conversion of fetus body, then enters the maternal blood circulation.According to the foreign study result, OES content in the time of pregnant the 16th day in sow blood plasma starts to raise, and reached peak value (3ng/ml) at 25 ~ 30 days, then in the time of 35 days, be reduced to below 1ng/ml again, and to maintain this level the 60th day be continue to raise to gestation; By contrast, the OES in in-pig blood plasma does not maintain reduced levels always, and therefore, the OES content in sow blood plasma or urine etc. can be used as the sign of early pregnancy diagnosis, and OES content is higher, and sow is cherished young number can be higher.
Summary of the invention
The application's purpose is to provide a kind of high sensitivity OES test strip, to solve the problem of the pregnant feelings Site Detection of sow in Swine Production.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of high sensitivity OES test strip, by micropore reagent (8) and test strips (9), formed, it is characterized in that: described micropore reagent (8) comprises backboard (1), post respectively from left to right the diaphragm paster (5-1) of tape label on described backboard (1), sample pad (2), cellulose membrane (3), adsorptive pads (4) and diaphragm paster (5-2), be respectively equipped with control line C line (7) and detection line T line (6) on described cellulose membrane (3), described C line (7) mark be the two anti-of sheep anti mouse, described T line (6) mark be the OES bond of bovine serum albumin white marker, be provided with OES monoclonal antibody layer (8-1) in described micropore reagent (8).
Further, 8 micropore reagent (8) are in a row by colloid stickup group mutually.
Further, described micropore reagent (8) also comprises plastic microporous (8-2), and covers the vinyl cover (8-3) on plastic microporous (8-2).
Further, described plastic microporous (8-2) is provided with the application of sample scale mark (8-4) as the application of sample volume.
Further, the width of described test strips (9) is 0.8cm, and deviation range is 0.78 ~ 0.82cm.
Further, the number of described micropore reagent (8) and test strips (9) is sealed in packaging bag or Packaging Bottle according to the ratio of 1:1, is kept at 4-8 ℃ of temperature.
The utility model patent can judge the become pregnant requirement of situation of pig by detecting the urine of discharging in 23 days after the pig fertilization, the situation that has that accuracy is high, simple to operate, the early detection pig is become pregnant, improve sow fertility efficiency, improve the characteristics such as income, be suitable for large-scale promotion application.
The accompanying drawing explanation
Fig. 1 test strips of the present utility model (9) structural plan vertical view
Fig. 2 test strips of the present utility model (9) structural profile schematic diagram
Fig. 3 micropore reagent of the present utility model (8) schematic diagram
Fig. 4 is micropore reagent (8) schematic diagram in a row
Fig. 5 testing result of the present utility model is judged schematic diagram
Backboard 1; Sample pad 2; Cellulose membrane 3; Adsorptive pads 4; The diaphragm paster 5-1 of tape label; T line 6; C line 7; Diaphragm paster 5-2; OES monoclonal antibody layer 8-1; Plastic microporous 8-2; Vinyl cover 8-3; Application of sample scale mark 8-4; Micropore reagent 8; Test strips 9
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.
The assembling of high sensitivity OES test strip
1. paste: the order by the diaphragm paster (5-1) of sample pad (2), cellulose membrane (3), adsorptive pads (4), tape label and diaphragm paster (5-2) with reference to Fig. 2 is attached on support backboard (1) one by one.Sample pad (2) is joined with cellulose membrane (3); cellulose membrane (3) joins with adsorptive pads (4); post respectively diaphragm paster (5-1) and the diaphragm paster (5-2) of tape label simultaneously on sample pad (2) and adsorptive pads (4); wherein the diaphragm paster (5-1) of the tape label of sample pad (2) is printed on " MAX " mark, and convenient the detection used.The upper mark respectively of cellulose membrane (3) control line C line (7) and detection line T line (6).C line (7) mark be the two anti-of sheep anti mouse, T line (6) mark be the OES bond of bovine serum albumin white marker.
2. slitting: the large plate of test strips (9) pasted is cut with cutting cutter, and the width of each test strips (9) is 0.8cm, and deviation range is 0.78 ~ 0.82cm, and the test strips of well cutting (9) storage is at room temperature standby.
3. the assembling of micropore reagent (8): adopt the quantitative OES of the high sensitivity by the nanogold particle mark monoclonal antibody of robotization sample adding device to be added in plastic microporous (8-2) and form OES monoclonal antibody layer (8-1), be placed in the freeze dryer freeze drying.Cryodesiccated micropore reagent (8) is built with vinyl cover (8-3), be stored in 4-8 ℃ standby, be provided with the application of sample scale mark (8-4) as the application of sample volume on plastic microporous (8-2), generally in a row in order conveniently to use 8 micropore reagent (8) to organize, for detecting.
4. the assembling of test strips (9) and micropore reagent (8): the ratio by the test strips (9) for preparing and micropore reagent (8) according to 1:1 is sealed in packaging bag or Packaging Bottle, after good seal, deposit in 4-8 ℃ standby.
The use of high sensitivity OES test strip
1. the preparation of sample:
Standby by after the urine sampling of pig to be detected, putting normal temperature, sample should be without precipitation and impurity.Sample the best of collection in worksite.
2. the preparation of test strips (9):
After quantity will need the high sensitivity OES test strip of use to give prominence to per sample, within standing 30 minutes, make it to return to room temperature, then the test strips of taking-up respective numbers (9) and micropore reagent (8) from pack from cold storage environment.All experimental implementation should operate on the experiment table of level or respective environment, and environment temperature is without particular restriction.
3. detect operation:
(1) application of sample: ready sample is drawn to 100 μ l with disposable plastic tube or micropipettor, join in the micropore reagent (8) that removes lid.Attention: the corresponding test strips (9) of a micropore reagent (8), can only detect a sample, can not reuse.
(2) mix: inhale up and down with micropipettor or disposable plastic tube the liquid of beating in micropore reagent (8) and make it fully to mix, the micropore after mixing should present pink or lightpink etc.
(3) transplant a cutting: take out test strips (9) and an end of the diaphragm paster (5-1) of tape label is inserted in micropore reagent (8).Please, not with the stage casing of finger or other objects touching test strips (9), contain cellulose membrane (3) section of detection line T line (6) and control line C line (7).
(4) timing: start timing after cutting 3 minutes, observe C line (7) and whether occur, if in 3min, C line (7) occurs, get final product sentence read result; If C line (7) does not occur, please extend 2 minutes and make liquid rise to the rear sentence read result of C line (7) appearance.
4. result is judged: Fig. 5
Negative: as shown in the 1st of Fig. 5, C line (7) colour developing, and T line (6) also develops the color, and T line (6) color is dark than C line (7);
Positive: C line (7) colour developing, T line (6) color is compared with C line (7) color:
(1) if T line (6) color than C line (7) shallow or T line (6) color and C line (7) nearer: as shown in the 2nd of Fig. 5, show that the concentration of OES in sample is between 40 μ g/L and 100 μ g/L.This sow of this presentation of results is successfully become pregnant.
(2), if T line (6) does not develop the color, as shown in the 3rd of Fig. 5, show that in sample, the concentration of OES is greater than 100 μ g/L.The sow that this presentation of results detects is successfully become pregnant, and it is higher to cherish young number.
Invalid: as shown in the 4th in Fig. 5 and 5, C line (7) does not show.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; the material that the utility model relates to is known materials; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (6)
1. a high sensitivity OES test strip, by micropore reagent (8) and test strips (9), formed, it is characterized in that: described micropore reagent (8) comprises backboard (1), post respectively from left to right the diaphragm paster (5-1) of tape label on described backboard (1), sample pad (2), cellulose membrane (3), adsorptive pads (4) and diaphragm paster (5-2), be respectively equipped with control line C line (7) and detection line T line (6) on described cellulose membrane (3), described C line (7) mark be the two anti-of sheep anti mouse, described T line (6) mark be the OES bond of bovine serum albumin white marker, be provided with OES monoclonal antibody layer (8-1) in described micropore reagent (8).
2. a kind of high sensitivity OES test strip according to claim 1 is characterized in that: 8 micropore reagent (8) are in a row by colloid stickup group mutually.
3. a kind of high sensitivity OES test strip according to claim 2, it is characterized in that: described micropore reagent (8) also comprises plastic microporous (8-2), and covers the vinyl cover (8-3) on plastic microporous (8-2).
4. a kind of high sensitivity OES test strip according to claim 3, it is characterized in that: described plastic microporous (8-2) is provided with the application of sample scale mark (8-4) as the application of sample volume.
5. a kind of high sensitivity OES test strip according to claim 4, it is characterized in that: the width of described test strips (9) is 0.8cm, deviation range is 0.78 ~ 0.82cm.
6. a kind of high sensitivity OES test strip according to claim 5, it is characterized in that: the number of described micropore reagent (8) and test strips (9) is sealed in packaging bag or Packaging Bottle according to the ratio of 1:1, is kept at 4-8 ℃ of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320541335.5U CN203376329U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity sulfuric acid oestrone |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320541335.5U CN203376329U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity sulfuric acid oestrone |
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| CN203376329U true CN203376329U (en) | 2014-01-01 |
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| CN201320541335.5U Expired - Fee Related CN203376329U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity sulfuric acid oestrone |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107192827A (en) * | 2017-07-13 | 2017-09-22 | 济南齐鲁医学检验有限公司 | A kind of anti-Miao Le pipes hormone（AMH）Detection means and method |
| CN108646021A (en) * | 2018-05-14 | 2018-10-12 | 新疆医科大学第附属医院 | Oestrone enzyme-linked immunologic detecting kit and its detection method and application |
-
2013
- 2013-09-02 CN CN201320541335.5U patent/CN203376329U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107192827A (en) * | 2017-07-13 | 2017-09-22 | 济南齐鲁医学检验有限公司 | A kind of anti-Miao Le pipes hormone（AMH）Detection means and method |
| CN108646021A (en) * | 2018-05-14 | 2018-10-12 | 新疆医科大学第附属医院 | Oestrone enzyme-linked immunologic detecting kit and its detection method and application |
| CN108646021B (en) * | 2018-05-14 | 2021-08-24 | 新疆医科大学第一附属医院 | Estrone enzyme-linked immunoassay kit, and detection method and application thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140101 Termination date: 20200902 |
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| CF01 | Termination of patent right due to non-payment of annual fee |