CN201021925Y - Colloid gold immune test paper for detecting progestin - Google Patents
Colloid gold immune test paper for detecting progestin Download PDFInfo
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- CN201021925Y CN201021925Y CNU2007200051475U CN200720005147U CN201021925Y CN 201021925 Y CN201021925 Y CN 201021925Y CN U2007200051475 U CNU2007200051475 U CN U2007200051475U CN 200720005147 U CN200720005147 U CN 200720005147U CN 201021925 Y CN201021925 Y CN 201021925Y
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- monoclonal antibody
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Abstract
The utility model relates to a colloidal gold immune indicator paper to detect luteohormone, which can detect outside a female body the function of a female epoophoron by detecting the level of luteohormone in human urine. The utility model belongs to the field of colloidal gold immune detection. The indicator paper comprises a bottom board, a water-absorbing board, a celluloid film, a monoclonal antibody gold labeled pad and a sample imbibition layer, wherein the center of the bottom board is provided with the celluloid film which is provided with a test line and a monoclonal antibody control line, one end of the bottom board is provided with the water-absorbing board and the other end is provided with the sample imbibition layer, the two ends of the celluloid film are respectively overlapped and connected with the water-absorbing board and the monoclonal antibody gold labeled pad, the sample imbibition layer is pressed on the monoclonal antibody gold labeled pad, and the indicator paper is made by the colloidal gold immune double-antibody sandwich method. The utility model has the advantages that the utility model is good in specificity, high in sensibility and easy to store, the result of the utility model is easy to read, and the utility model can be operated without professional technicians.
Description
Technical field
The present invention relates to a kind of progesterone level that passes through in the human body urine, judge women's ovarian function, belong to colloid gold immune diagnostic techniques field.
Background technology
Progesterone is the steroid hormone of 21 carbon atoms, and molecular weight is 314.5.Progesterone is regulated relevant accessory organ's function with the menstrual cycle together with estrogen, particularly importantly keeps gestation and prepares to endometrium for the blastocyte implantation.Mainly by the corpus luteum secretion, the pregnant woman is then mainly by placenta secretion for non-pregnant women's progesterone.The adrenal cortex of men and women's both sexes and the male sex's testis are also secreted a small amount of progesterone.Main and the corticosteroid-binding globulin (CBG) of progesterone in the blood circulation, sex hormone binding globulin (SHBG) and albumin bound and transport, in all round-robin progesterone, having only 2-10% is free form.
In the blood and urine in progesterone concentration with the menstrual cycle significant change is arranged.Follicular phase is less than 1ng/ml (3.2mol/L), is that 10-20ng/ml (32-64mol/L) highest level is after ovulation 4～7 days in luteal phase, and continues to keep high level 4～6 days level before menstruation rolled back ovulation in preceding 24 hours.Because the lifting of progesterone level is relevant with ovarian follicle and luteal function, measure that blood plasma or urine progesterone level are clinical to be used to judge the luteal function that anovulation and non-pregnant woman are arranged.If not ovulation, corpus luteum does not form, and does not just have the periodicity of plasma progesterone level to raise.Unusual progesterone secretion and premenstrual tension, in utero film irregularly strips off, dysmenorrhoea is relevant with luteal phase defect.
The assay method of progesterone has gas chromatography, protein binding analysis method and radioimmunology and chemoluminescence method.Because progesterone content is lower in the blood, using more method at present is immuno-enzymatic connection determination method (IEMA) and the luminescence method that protein binding analysis, radio immunoassay (RIA) and magnetic particle separate.These methods need special safeguard, harsh condition, expensive instrument and well-trained personnel, and during operating cost, the sample storage difficulty, be not easy to apply.
Immunoassay provides a new analyzing and testing approach for the detection of progesterone, and existing having utilizes the immune colloid gold method to make the report of test paper: Chinese patent 200510109165.3 is used for the test paper and the detection method thereof of milk cow diagnosis of early gestation.This invention is for detecting the progesterone content in the dairy cow milk of breeding back, the detection test paper and the detection method thereof of field diagnostic milk cow's early pregnancy by the colloid gold immune method.The present invention is for passing through the progesterone in the human body urine, and external qualitative detection women's ovarian function, technical characterstic and purposes are all different, do not constitute infringement.
Summary of the invention
The present invention has overcome some shortcomings that exist in the prior art, provides a kind of by the progesterone in the colloid gold immune method human body urine, thus external qualitative detection women's ovarian function.
The present invention is achieved by the following technical solutions:
At progesterone colloidal gold diagnosis test paper base plate middle part is nitrocellulose filter, a test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the urine liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water accepting layer respectively and are connected (the overlapping coupling part is in 1-2 millimeter scope), are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
A test wire and a polyclonal antibody control line are arranged on the nitrocellulose filter.Control line is to be made into by sheep anti mouse polyclonal antibody bag; Go out at a kind of immunogenic two kinds of pairing monoclonal antibodies by immunoscreening, a kind of monoclonal antibody I that is called is used for bag by test wire, another kind is called monoclonal antibody II and is used for colloid gold label, common and immune original affinity interaction, the test paper that utilizes double antibodies sandwich method principle to make.
The sample liquid-adsorption layer end of progesterone colloidal gold diagnosis test paper is put into urine to be measured (liquid level must not surpass the MAX line), because the capillarity urine will move along test strips water accepting layer end, in up process, progesterone in the urine and antiprogestin monoclonal antibody gold mark probe generation specific bond, when moving to the test wire that is fixed with progesterone monoclonal antibody I, another site of progesterone in the urine combines with progesterone monoclonal antibody I in the test wire again, therefore its collaurum is stranded on the test wire, the test wire place shows red, and is positive; If do not have progesterone in the opposite urine, specific bond just can not take place with the progesterone monoclonal antibody I on the test wire in antiprogestin monoclonal antibody gold mark probe, does not have collaurum to be detained.Promptly only there is a red control line negative, double antibodies sandwich method principle that Here it is.According to this principle, two lines are positive, and line is negative to draw diagnosis.
When moving to sheep anti mouse polyclonal antibody control line, no matter have or not corresponding antigens in the urine, the gold mark probe of mark all can combine delay with the sheep anti mouse polyclonal antibody that has configured, and it is red that control line is shown.Therefore control line does not have colour band and produces that then the representative operation is wrong, during detection the urine liquid level surpass the MAX line or test paper expired.
Because adopt technique scheme, the colloid gold immune test paper of detection progesterone provided by the present invention has such beneficial effect, promptly high specificity is highly sensitive, easily stores, need not the technical skill personnel operation, and readability as a result.
Description of drawings
Fig. 1 is main TV structure figure of the present invention.
Fig. 2 is the sectional structure chart of overlooking of Fig. 1.
The negative figure as a result of Fig. 3.
The positive figure as a result of Fig. 4.
Among the figure 1, water sucting plate, 2, nitrocellulose filter, 3, sheep anti mouse polyclonal antibody control line, 4, test wire, 5, monoclonal antibody gold mark pad, 6, the urine liquid-adsorption layer, 7, base plate, 8, the MAX line.
Specific embodiment
1. the progesterone immunogene of the synthetic two kinds of different binding sites of chemical synthesis process (progesterone-BSA).
2. the preparation of progesterone monoclonal antibody.
The progesterone of two kinds of different binding sites that (1) will prepare-BSA is immune BALB/c mouse respectively.Set up the knurl strain with the SP20 Fusion of Cells and screen, get oncocyte and be injected in the BALB/c mouse abdominal cavity, make it produce ascites.
(2) extract the screening of mouse ascites purifying, obtain the two kinds of progesterone monoclonal antibody I, the progesterone monoclonal antibody II that combine with progesterone molecule different loci.
The preparation of collaurum and with the combining of progesterone monoclonal antibody.
(1) get distilled water and add an amount of gold chloride magnetic agitation and be warmed to 90～95 ℃, add an amount of citric acid three and receive and continue heated and stirred to seething with excitement 5 minutes, it is standby to keep in Dark Place after the cooling.
(2) the collaurum liquid of progesterone monoclonal antibody mark, on the adsorbing fiber material, dry back is standby.
4. film-making machine system film: utilize computer control transmission speed, guarantee that the antibody amount of bag quilt on the per unit film equates.
5. test strips combination: at progesterone colloidal gold diagnosis test paper base plate middle part is nitrocellulose filter, a test wire and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water accepting layer, other end termination is the urine liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water accepting layer respectively and are connected (the overlapping coupling part is in 1-2 millimeter scope), are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
6. using method: collect urina sanguinis, test paper liquid-adsorption layer end is inserted in the urine, the sample to be tested interface surpasses " MAX " line on the diagnose test paper, observations in 5 minutes.The colour developing district is two red line, and detection line is darker or equal than control line color, and then testing result is positive; It is negative when response line is shallower than control line or a control line only occurs; Colour band does not appear in control line, shows the expired or misoperation of test paper, please gets test paper in addition and detects again.
Claims (2)
1. a colloid gold immune test paper that detects progesterone is characterized in that being made up of base plate (7), water sucting plate (1), nitrocellulose filter (2), monoclonal antibody gold mark pad (5), sample liquid-adsorption layer (6), MAX line (8); The base plate middle part is a nitrocellulose filter, a test wire (4) and a polyclonal antibody control line (3) are arranged on the nitrocellulose filter, base plate one end termination is a water sucting plate, other end termination is the sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the sample liquid-adsorption layer on monoclonal antibody gold mark pad.
2. a kind of colloid gold immune test paper that detects progesterone according to claim 1 is characterized in that control line is formed by sheep anti mouse polyclonal antibody bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200051475U CN201021925Y (en) | 2007-02-14 | 2007-02-14 | Colloid gold immune test paper for detecting progestin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200051475U CN201021925Y (en) | 2007-02-14 | 2007-02-14 | Colloid gold immune test paper for detecting progestin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201021925Y true CN201021925Y (en) | 2008-02-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200051475U Expired - Lifetime CN201021925Y (en) | 2007-02-14 | 2007-02-14 | Colloid gold immune test paper for detecting progestin |
Country Status (1)
| Country | Link |
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| CN (1) | CN201021925Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297966A (en) * | 2011-05-27 | 2011-12-28 | 天津农学院 | Progesterone semi-quantitative determining colloidal gold test paper and preparation method thereof |
| CN109813920A (en) * | 2017-11-20 | 2019-05-28 | 丹阳亿太生物科技发展有限公司 | A kind of remaining Rapid detection test strip of detection progesterone |
-
2007
- 2007-02-14 CN CNU2007200051475U patent/CN201021925Y/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297966A (en) * | 2011-05-27 | 2011-12-28 | 天津农学院 | Progesterone semi-quantitative determining colloidal gold test paper and preparation method thereof |
| CN109813920A (en) * | 2017-11-20 | 2019-05-28 | 丹阳亿太生物科技发展有限公司 | A kind of remaining Rapid detection test strip of detection progesterone |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20080213 |