CN203376327U - Test strip for testing high-sensitivity aflatoxin M1 - Google Patents
Test strip for testing high-sensitivity aflatoxin M1 Download PDFInfo
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- CN203376327U CN203376327U CN201320541329.XU CN201320541329U CN203376327U CN 203376327 U CN203376327 U CN 203376327U CN 201320541329 U CN201320541329 U CN 201320541329U CN 203376327 U CN203376327 U CN 203376327U
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- aflatoxin
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- test strip
- high sensitivity
- micropore reagent
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Abstract
A test strip for testing high-sensitivity aflatoxin M1 is composed of a microvoid reagent (8) and a test strip body (9). The test strip is characterized in that the microvoid reagent (8) comprises a back plate (1), a protective film sticker (5-1), a sample pad (2), a cellulose membrane (3), a water absorption pad (4) and a protective film sticker (5-2) are respectively pasted on the back plate (1) from left to right, wherein the protective film sticker (5-1), the sample pad (2), the cellulose membrane (3), the water absorption pad (4) and the protective film sticker (5-2) are respectively provided with a mark, a control line C line (7) and a test line T line (6) are arranged on the cellulose membrane (3) respectively, wherein the C line (7) marks a second antibody of goat antimouse or rabbit antimouse, the T line (6) marks a compound of the aflatoxin M1 and a protein carrier, and an aflatoxin M1 monoclonal antibody gold nanoparticle layer (8-1) is filled into the microvoid reagent (8). The test strip is high in sensitivity, wide in test range, rapid in test speed, and convenient to operate.
Description
Technical field
The present invention relates to detect the reagent field, be specially a kind of high sensitivity aflatoxin M 1 test strip.
Background technology
Aflatoxin (aflatoxin), it is a kind of compound that strong bio-toxicity is arranged, often by aspergillus flavus and other several mould, in the cereal gone mouldy, produced, as rice, beans, peanut etc., be that the strongest so far carcinogen is one of chemical substance of bibliographical information toxicity maximum up to now.Aflatoxin mainly comprises aflatoxin B1, B2, G1, G2 and other two kinds of metabolic product M1 and M2.If in animal husbandry cultivation practice the milk cattle fooder after aflatoxin-contaminated feed, most of aflatoxin can be aflatoxin M 1 at the Contents in Cows intracellular metabolite, and exist in cow's milk juice, then eaten by the people and produce harm, especially larger for take baby's harm that milk is Major Foods.Countries in the world have all been made regulation to aflatoxin M 1 in the maximum residue limit of fresh milk at present, and wherein European Union is 0.05 μ g/L, and the U.S. and China are 0.5 μ g/L.
The detection of aflatoxin M 1 at present mainly relies on enzyme-linked immunologic detecting kit method, fluorometry, liquid-phase chromatography method and liquid chromatography mass method for combined use etc.These method detectabilities can meet national limit standard requirement, but majority depends on large-sized analytic instrument as high performance liquid chromatography, LC-MS instrument and fluorophotometer, microplate reader etc., are mainly used in the inner detection in laboratory.And China milk industry industry cultivation large-scale degree is not high at present; milk need to be collected from casual household or small scale producers by most enterprises; therefore be necessary to develop a kind of detection method of applying while being applicable to on-site collection and products thereof, meet the actual detection demand of basic unit's milking station and enterprise.
Summary of the invention
The application's purpose is to provide a kind of high sensitivity aflatoxin M 1 test strip, to solve the problem that quick and precisely detects fresh milk aflatoxin M 1 in the practical application of current basic unit.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of high sensitivity aflatoxin M 1 test strip, by micropore reagent (8) and test strips (9), formed, it is characterized in that: described micropore reagent (8) comprises backboard (1), post respectively from left to right the diaphragm paster (5-1) of tape label on described backboard (1), sample pad (2), cellulose membrane (3), adsorptive pads (4) and diaphragm paster (5-2), be respectively equipped with control line C line (7) and detection line T line (6) on described cellulose membrane (3), described C line (7) mark be the two anti-of sheep anti mouse or the anti-mouse of rabbit, described T line (6) mark be aflatoxin M 1 and the conjugate of protein carrier, aflatoxin M 1 monoclonal antibody nanogold particle layer (8-1) is housed in described micropore reagent (8).
Further, described micropore reagent (8) also comprises plastic microporous (8-2), and covers the vinyl cover (8-3) on plastic microporous (8-2).
Further, described plastic microporous (8-2) is provided with the application of sample scale mark (8-4) as the application of sample volume.
Further, 8 of the every rows of described micropore reagent (8), be pasted together by fixing glue each other.
Further, the width of described test strips (9) is 0.8cm, and deviation range is 0.78 ~ 0.82cm.
Further, the number of described micropore reagent (8) and test strips (9) is sealed in packaging bag or Packaging Bottle according to the ratio of 1:1, is kept at 4-8 ℃ of temperature.
Beneficial effect of the present invention is:
1. highly sensitive, sensing range is wide.Test strips (9) be take aflatoxin M 1 high sensitivity monoclonal antibody and is developed as basic material, can be for detection of the aflatoxin M 1 of fresh milk, detectability is minimum can reach 0.05 μ g/L, the aflatoxin M 1 that meets European Union, the U.S. and the China requirement of limiting the quantity of.
2. detection speed is fast, easy to operate.Test strips (9) the experiment reaction time is only 3 minutes, easy and simple to handle, without Special Training.
3. testing result is judged intuitively, without specific apparatus.
4. reduce costs cost saving.Because test strips (9) has been applied highly sensitive monoclonal antibody, the cost of single test strips (9) research and production greatly reduces, and comparing price with the foreign same type product is only 1/3rd.
The accompanying drawing explanation
Fig. 1 test strips of the present utility model (9) structural plan vertical view
Fig. 2 test strips of the present utility model (9) structural profile schematic diagram
Fig. 3 micropore reagent of the present utility model (8) schematic diagram
Fig. 4 is micropore reagent (8) schematic diagram in a row
Fig. 5 testing result of the present utility model is judged schematic diagram
Backboard 1; Sample pad 2; Cellulose membrane 3; Adsorptive pads 4; The diaphragm paster 5-1 of tape label; T line 6; C line 7; Diaphragm paster 5-2; Aflatoxin M 1 monoclonal antibody nanogold particle layer 8-1; Plastic microporous 8-2; Vinyl cover 8-3; Application of sample scale mark 8-4; Micropore reagent 8; Test strips 9
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.
The assembling of high sensitivity aflatoxin M 1 test strip
1. paste: the order by the diaphragm paster (5-1) of sample pad (2), cellulose membrane (3), adsorptive pads (4), tape label and diaphragm paster (5-2) with reference to Fig. 2 is attached on support backboard (1) one by one.Sample pad (2) is joined with cellulose membrane (3); cellulose membrane (3) joins with adsorptive pads (4); post respectively diaphragm paster (5-1) and the diaphragm paster (5-2) of tape label simultaneously on sample pad (2) and adsorptive pads (4); wherein the diaphragm paster (5-1) of the tape label of sample pad (2) is printed on " MAX " mark, and convenient the detection used.The upper mark respectively of cellulose membrane (3) control line C line (7) and detection line T line (6).C line (7) mark be the two anti-of sheep anti mouse or the anti-mouse of rabbit, and T line (6) mark is aflatoxin M 1 bond of bovine serum albumin white marker.
2. slitting: the large plate of test strips (9) pasted is cut with cutting cutter, and the width of each test strips (9) is 0.8cm, and deviation range is 0.78 ~ 0.82cm, and the test strips of well cutting (9) storage is at room temperature standby.
3. the assembling of micropore reagent (8): adopt quantitative aflatoxin M 1 monoclonal antibody nanogold particle layer (8-1) is added in plastic microporous (8-2) of robotization sample adding device, be placed in the freeze dryer freeze drying.Cryodesiccated micropore reagent (8) is built with vinyl cover (8-3), be stored in 4-8 ℃ standby, be provided with the application of sample scale mark (8-4) as the application of sample volume on plastic microporous (8-2), generally in a row in order conveniently to use 8 micropore reagent (8) to organize, for detecting.
4. the assembling of test strips (9) and micropore reagent (8): the ratio by the test strips (9) for preparing and micropore reagent (8) according to 1:1 is sealed in packaging bag or Packaging Bottle, after good seal, deposit in 4-8 ℃ standby.
The use of high sensitivity aflatoxin M 1 test strip
1. the preparation of sample:
To after fresh milk sampling to be detected, return to room temperature, sample should be without precipitation and impurity, too thickness.Sample the best of collection in worksite.
2. the preparation of test strips (9):
After quantity will need the test strips (9) of use to give prominence to per sample, within standing 30 minutes, make it to return to room temperature, then the test strips of taking-up respective numbers (9) and micropore reagent (8) from pack from cold storage environment.All experimental implementation should operate on the experiment table of level or respective environment, and environment temperature is without particular restriction.
3. detect operation:
(1) application of sample: ready sample is drawn to 200 μ l with disposable plastic tube or micropipettor, join in the micropore reagent (8) that removes lid.Attention: the corresponding test strips (9) of a micropore reagent (8), can only detect a sample, can not reuse.
(2) mix: inhale up and down with micropipettor or disposable plastic tube the liquid of beating in micropore reagent (8) and make it fully to mix, the micropore after mixing should present pink or lightpink etc.
(3) transplant a cutting: take out test strips (9) and an end of the diaphragm paster (5-1) of tape label is inserted in micropore reagent (8).Please, not with the stage casing of finger or other objects touching test strips (9), contain cellulose membrane (3) section of detection line T line (6) and control line C line (7).
(4) timing: start timing after cutting 3 minutes, observe C line (7) and whether occur, if in 3min, C line (7) occurs, get final product sentence read result; If C line (7) does not occur, please extend 2 minutes and make liquid rise to the rear sentence read result of C line (7) appearance.
4. result is judged: Fig. 5
Negative: as shown in the 1st of Fig. 5, C line (7) colour developing, and T line (6) also develops the color, and T line (6) color is dark than C line (7);
Positive: C line (7) colour developing, T line (6) color is compared with C line (7) color:
(1) if T line (6) color is shallow than C line (7): as shown in the 2nd of Fig. 5, show that the concentration of aflatoxin M 1 in sample is between 0.05 μ gL and 0.5 μ gL.Belong to positive according to European Union's standard judgement, and belong to positive according to the judgement of China and U.S.A standard.
(2), if T line (6) does not develop the color, as shown in the 3rd of Fig. 5, show that the concentration of aflatoxin M 1 in sample is greater than 0.5 μ gL.All belong to positive according to European Union, the judgement of China and U.S.A standard.
Invalid: the 4th and 5 shown in, C line (7) does not show as in Fig. 5.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (6)
1. high sensitivity aflatoxin M 1 test strip, by micropore reagent (8) and test strips (9), formed, it is characterized in that: described micropore reagent (8) comprises backboard (1), post respectively from left to right the diaphragm paster (5-1) of tape label on described backboard (1), sample pad (2), cellulose membrane (3), adsorptive pads (4) and diaphragm paster (5-2), be respectively equipped with control line C line (7) and detection line T line (6) on described cellulose membrane (3), described C line (7) mark be the two anti-of sheep anti mouse or the anti-mouse of rabbit, described T line (6) mark be aflatoxin M 1 and the bond of protein carrier, aflatoxin M 1 monoclonal antibody nanogold particle layer (8-1) is housed in described micropore reagent (8).
2. a kind of high sensitivity aflatoxin M 1 test strip according to claim 1, it is characterized in that: described micropore reagent (8) also comprises plastic microporous (8-2), and covers the vinyl cover (8-3) on plastic microporous (8-2).
3. a kind of high sensitivity aflatoxin M 1 test strip according to claim 2, it is characterized in that: described plastic microporous (8-2) is provided with the application of sample scale mark (8-4) as the application of sample volume.
4. a kind of high sensitivity aflatoxin M 1 test strip according to claim 3, is characterized in that: described micropore reagent (8 8 of every rows.
5. according to described a kind of high sensitivity aflatoxin M 1 test strip of claim 1-4, it is characterized in that: the width of described test strips (9) is 0.8cm, and deviation range is 0.78 ~ 0.82cm.
6. a kind of high sensitivity aflatoxin M 1 test strip according to claim 5, it is characterized in that: the number of described micropore reagent (8) and test strips (9) is sealed in packaging bag or Packaging Bottle according to the ratio of 1:1, is kept at 4-8 ℃ of temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320541329.XU CN203376327U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity aflatoxin M1 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320541329.XU CN203376327U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity aflatoxin M1 |
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| CN203376327U true CN203376327U (en) | 2014-01-01 |
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| CN201320541329.XU Expired - Fee Related CN203376327U (en) | 2013-09-02 | 2013-09-02 | Test strip for testing high-sensitivity aflatoxin M1 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198966A (en) * | 2016-09-06 | 2016-12-07 | 中国农业大学 | A kind of near-infrared fluorescent immune chromatography reagent kit detecting aflatoxin and application thereof |
| CN106526171A (en) * | 2015-09-11 | 2017-03-22 | 睿嘉生物科技股份有限公司 | Immune detection kit |
-
2013
- 2013-09-02 CN CN201320541329.XU patent/CN203376327U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106526171A (en) * | 2015-09-11 | 2017-03-22 | 睿嘉生物科技股份有限公司 | Immune detection kit |
| CN106198966A (en) * | 2016-09-06 | 2016-12-07 | 中国农业大学 | A kind of near-infrared fluorescent immune chromatography reagent kit detecting aflatoxin and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140101 Termination date: 20200902 |