CN203310791U - Rapid-assembly micro-circulation detection pool device with magnetic separation - Google Patents
Rapid-assembly micro-circulation detection pool device with magnetic separation Download PDFInfo
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- CN203310791U CN203310791U CN2013203157208U CN201320315720U CN203310791U CN 203310791 U CN203310791 U CN 203310791U CN 2013203157208 U CN2013203157208 U CN 2013203157208U CN 201320315720 U CN201320315720 U CN 201320315720U CN 203310791 U CN203310791 U CN 203310791U
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- pipe
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- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 230000004089 microcirculation Effects 0.000 title claims abstract description 8
- 238000007885 magnetic separation Methods 0.000 title abstract description 8
- 230000004087 circulation Effects 0.000 claims abstract description 19
- 239000012530 fluid Substances 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000006148 magnetic separator Substances 0.000 claims description 16
- 238000004140 cleaning Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000002203 pretreatment Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000012491 analyte Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
The utility model discloses a rapid-assembly micro-circulation detection pool device with magnetic separation, which comprises a circulation detection pool device and a magnetic separation frame, wherein the circulation detection pool device comprises a cover plate, a bottom plate, a sealing element, a detection chip, an interface plate, a magnet, pipeline connectors and pipelines; the pipelines are mounted at a fluid inlet and a fluid outlet of the cover plate respectively by the two pipeline connectors; a sealing slot is formed in the bottom surface of the cover plate and used for mounting the sealing element; the sealing slot is positioned below the two pipeline connectors; an interface plate slot is formed in the bottom surface of the cover plate and used for fixing the interface plate; the interface plate is in elastic contact with an electric connection point of the detection chip; a chip slot is formed in the top surface of the bottom plate and used for embedding the detection chip; a magnet is embedded in the bottom surface of the cover plate; another magnet is embedded in the top surface of the bottom plate; and the cover plate and the bottom plate are close to each other for sealing through the opposite attraction between the upper magnet and the lower magnet respectively. The device disclosed by the utility model has the advantages of high mounting speed, simple structure, advanced functions, convenience in cleaning and the like, and can be widely applied to the fields such as biology, chemistry, life science and the like.
Description
Technical field
The utility model relates to the analyzing and testing field, relates in particular to a kind of micro-circulation detecting pool device of quick assembling with the magnetic separation.
Background technology
The logical pick-up unit of miniflow is the product of micromachining technology and microelectric technique development, it have detection sensitivity high, to advantages such as the consumption of sample are few, in fields such as biology, chemistry, life sciences, be widely used.The logical pick-up unit of miniflow generally includes micro-detection chip and flow cell.How to be micro-detection chip and flow cell combination a kind of important technical matters.Commonly used is, by certain method, microelectrode and flow cell are combined into to a non-removable integral body at present, and this method is high to processing request, and causes the expensive of chip.If seal bad just easily causing, leak, or installation site is not right, easily causes integral body to be scrapped yet, and exists difficult cleaning of device easily to cause residual cross pollution, the problem such as access times are on the low side.Another mode is to adopt a minute cube method, microelectrode and detection cell can be dismantled, as described as patent (200710070709.9, multifunctional micro-electrode chip circulation detecting pool device), this design generally is comprised of top board, elastic membrane, lower platen, micro-electrode chip etc.After assembling, just tighten and can use by set bolt, have conveniently to clean, reuse and wait often advantage.But exist the degree of tightening of set bolt to vary with each individual, and the shortcoming such as bolt tightening is more loaded down with trivial details.In addition, in the analyzing and testing field, immune magnetic separation technique is widely used as a kind of effective pretreatment technology.It is to utilize antigen and antibody specific in conjunction with carrying out target acquisition, then utilizes the quickly separating target that is used in magnetic field.Usually in analyte detection process, need to be equipped with the pre-treatment that special magnetic separating apparatus carries out sample, use inconvenient.
Summary of the invention
The purpose of this utility model is for the deficiencies in the prior art, and a kind of micro-circulation detecting pool device of quick assembling with the magnetic separation is provided.
The technical solution adopted in the utility model is as follows: a kind of micro-circulation detecting pool device of quick assembling with the magnetic separation, and it comprises circulation detecting pool device and magnetic separator frame; Wherein, described circulation detecting pool device comprises cover plate, base plate, seal, detection chip, interface board, the first magnet, the second magnet, the 3rd magnet, the 4th magnet, the 5th magnet, the 6th magnet, the 7th magnet, the 8th magnet, the first pipe jointer, second pipe connector and the first pipeline, second pipe; The cover plate end face has fluid intake and fluid egress point, and the first pipe jointer, second pipe connector are threaded connection fluid intake and the fluid outlet that respectively the first pipeline, second pipe is arranged on to cover plate; There is seal groove the bottom surface of cover plate be used to seal is installed, and seal groove is positioned at the below of the first pipe jointer, second pipe connector; Seal is in the detection zone top of detection chip; There is the interface board slot cover plate bottom surface for the fixed interface plate; The end face of base plate has chip groove setting-in detection chip; The electric connecting point Elastic Contact of interface board and detection chip; Bottom surface setting-in first magnet of cover plate, the second magnet, the 3rd magnet, the 4th magnet; End face setting-in the 5th magnet of base plate, the 6th magnet, the 7th magnet, the 8th magnet; There is a natural attraction between the sexes presses close to sealing by the first magnet, the second magnet, the 3rd magnet, the 4th magnet and the 5th magnet, the 6th magnet, the 7th magnet, the 8th magnet respectively for cover plate and base plate; The magnetic separator frame is inlaid on the cover plate of circulation detecting pool device, the magnetic separator frame has the first base hole, the second base hole, the 3rd base hole, the 4th base hole, the 5th base hole, the 6th base hole, the 7th base hole, the 8th base hole, the 9th base hole, the tenth base hole, the 11 base hole, the 12 base hole, separates pipe for fixing magnetic.
Further, the angle between described the first pipe jointer, second pipe connector and normal is 0 degree ~ 90 degree.
The beneficial effect the utlity model has is: the utility model multifunctional micro-electrode chip circulation detecting pool organically combines magnetic based Sample Pretreatment Technique and pick-up unit, have the advantages such as installation and removal are quick, simple in structure, multi-functional, cleaning is convenient, can be widely used in fields such as analyzing and testing, can replace the complicated pick-up unit based on precision processing technology, but all widespread uses in life science, chemical analysis, food security, environmental monitoring and relevant scientific domain.
The accompanying drawing explanation
Fig. 1 is system schematic of the present utility model;
Fig. 2 is upper plate backplan of the present utility model;
Fig. 3 is base plate vertical view of the present utility model;
Fig. 4 is magnetic separator frame vertical view of the present utility model.
In figure, cover plate 1, base plate 2, seal 3, detection chip 4, interface board 5, magnetic separator frame 6, the first pipe jointer 7, second pipe connector 8, the first magnet 9, the second magnet 10, the 3rd magnet 11, the 4th magnet 12, the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16, the first pipeline 17, second pipe 18, the first fixed orifice 101, the second fixed orifice 102, the 3rd fixed orifice 103, the 4th fixed orifice 104, fluid intake 105, fluid egress point 106, interface board slot 107, seal channel 108, the 5th fixed orifice 201, the 6th fixed orifice 202, the 7th fixed orifice 203, the 8th fixed orifice 204, chip groove 205, the first base hole 601, the second base hole 602, the 3rd base hole 603, the 4th base hole 604, the 5th base hole 605, the 6th base hole 606, the 7th base hole 607, the 8th base hole 608, the 9th base hole 609, the tenth base hole 610, the 11 base hole 611, the 12 base hole 612, circulation detecting pool 19.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples.
As shown in Figure 1 to 4, the utility model comprises circulation detecting pool device 19 and magnetic separator frame 6.
Circulation detecting pool device 19 comprises cover plate 1, base plate 2, seal 3, detection chip 4, interface board 5, the first magnet 9, the second magnet 10, the 3rd magnet 11, the 4th magnet 12, the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16, the first pipe jointer 7, second pipe connector 8 and the first pipeline 17, second pipe 18.
Cover plate 1 end face has fluid intake 105 and fluid egress point 106, the first pipe jointers 7, second pipe connector 8 to be threaded connection fluid intake 105 and fluid egress point 106 places that respectively the first pipeline 17, second pipe 18 are arranged on to cover plate; There is seal groove 108 bottom surface of cover plate 1 be used to seal 3 is installed, and seal groove 108 is positioned at the below of the first pipe jointer 7, second pipe connector 8.Seal 3 is in the detection zone top of detection chip 4.
There is interface board slot 107 cover plate 1 bottom surface for fixed interface plate 5.The end face of base plate 2 has chip groove 205 setting-in detection chip 4.The electric connecting point Elastic Contact of interface board 5 and detection chip 4.
Bottom surface setting-in first magnet 9 of cover plate 1, the second magnet 10, the 3rd magnet 11, the 4th magnet 12.
End face setting-in the 5th magnet 13 of base plate 2, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16.
There is a natural attraction between the sexes presses close to sealing by the first magnet 9, the second magnet 10, the 3rd magnet 11, the 4th magnet 12 and the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16 respectively for cover plate 1 and base plate 2.
Angle between described the first pipe jointer 7, second pipe connector 8 and normal is 0 degree ~ 90 degree.
Application process of the present utility model is as follows:
1, the 5th fixed orifice 201, the 6th fixed orifice 202, the 7th fixed orifice 203, the 8th fixed orifice 204 setting-ins the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16 of base plate 2 end faces, the end face of detection chip 4 being put into to base plate 2 has chip groove 205.
The polarity of 2, the first fixed orifice 101, the second fixed orifice 102, the 3rd fixed orifice 103, the 4th fixed orifice 104 setting-in the first magnet 9, the second magnet 10, the 3rd magnet 11, the 4th magnet 12, the first magnet 9, the second magnet 10, the 3rd magnet 11, the 4th magnet 12 of cover plate 1 bottom surface being pressed close to cover plate 1 bottom surface is opposite with the polarity that the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the 8th magnet 16 are pressed close to plate top surface.The first pipe jointer 7, second pipe connector 8 are threaded connection to fluid intake 105 and fluid egress point 106 places that respectively the first pipeline 17, second pipe 18 are arranged on to cover plate 1.Seal 3 is put into to the seal groove 108 of cover plate 1, interface board 5 is screwed in the interface board slot 107 of cover plate 1 bottom surface.
3, below mounted base plate 2 is placed on, above mounted cover plate 1 is placed on.Cover plate 1 near base plate 2, after because the effect of magnetic force just is assembled into circulation detecting pool device 19 to cover plate 1 and base plate 2 auto-alignments.
4, the test cable interface is connected with the interface board 5 of circulation detecting pool device 19 with an end, the other end is connected with the signal input part of detecting instrument.
5, detected sample is expelled to pipeline 17, and flows in the detection space that the detection zone of detection chip 4 and seal 3 form, laggard pipeline 18 flows out.Read the electrical signal of detecting instrument and just can know the characteristic of detected sample.
6, cleaning electrode only need clean seal 3, detection chip 4 and cover plate 1 and corresponding pipeline cover plate 1 with after base plate 2 directly separates, and can reuse chip like this.
7, by changing the shape and size of seal 3, just can change the volume of circulation detecting pool.
If 8 need to carry out immune magnetic to testing sample, separate pre-treatment, the immunomagnetic beads of modified target antibody to be measured is joined in the magnetic separator tube.The magnetic separator tube is built to rear use hand even, make the magnetic bead of modified good antibody react and combine with the testing sample developmental biology, again the magnetic separator tube is put into to the first base hole 601, the second base hole 602, the 3rd base hole 603, the 4th base hole 604, the 5th base hole 605, the 6th base hole 606, the 7th base hole 607, the 8th base hole 608 is in any one, due to the first magnet 9 in circulation detecting pool device 19, the second magnet 10, the 3rd magnet 11, the 4th magnet 12, the 5th magnet 13, the 6th magnet 14, the 7th magnet 15, the directional magnetic force effect of magnetic bead in the 8th 16 pairs of magnet pipe and make the magnetic bead the magnetic separator tube in and be close to the inwall gathering by circulation detecting pool device 19 these sides with target to be measured, with pipettor, liquid is siphoned away, and magnetic bead and be subject to magneticaction and continue to be adsorbed on tube wall, add again damping fluid in the magnetic separator tube, so just completed the pre-treatment operation that utilizes magnetic to separate.
If 7 pipe or the reagent that do not need magnetic to separate, can be placed on the 9th base hole 609, the 611, the 12 base hole 612, the 610, the 11 base hole, the tenth base hole.
Claims (2)
1. micro-circulation detecting pool device of quick assembling separated with magnetic, it is characterized in that: it comprises circulation detecting pool device (19) and magnetic separator frame (6); Wherein, described circulation detecting pool device (19) comprises cover plate (1), base plate (2), seal (3), detection chip (4), interface board (5), the first magnet (9), the second magnet (10), the 3rd magnet (11), the 4th magnet (12), the 5th magnet (13), the 6th magnet (14), the 7th magnet (15), the 8th magnet (16), the first pipe jointer (7), second pipe connector (8) and the first pipeline (17), second pipe (18); Cover plate (1) end face has fluid intake (105) and fluid egress point (106), and the first pipe jointer (7), second pipe connector (8) are threaded connection respectively fluid intake (105) and fluid egress point (106) that the first pipeline (17), second pipe (18) are arranged on to cover plate and locate; There is seal groove (108) bottom surface of cover plate (1) be used to seal (3) is installed, and seal groove (108) is positioned at the below of the first pipe jointer (7), second pipe connector (8); Seal (3) is in the detection zone top of detection chip (4); There is interface board slot (107) cover plate (1) bottom surface for fixed interface plate (5); The end face of base plate (2) has chip groove (205) setting-in detection chip (4); The electric connecting point Elastic Contact of interface board (5) and detection chip (4); Bottom surface setting-in first magnet (9) of cover plate (1), the second magnet (10), the 3rd magnet (11), the 4th magnet (12); End face setting-in the 5th magnet (13) of base plate (2), the 6th magnet (14), the 7th magnet (15), the 8th magnet (16); There is a natural attraction between the sexes presses close to sealing by the first magnet (9), the second magnet (10), the 3rd magnet (11), the 4th magnet (12) and the 5th magnet (13), the 6th magnet (14), the 7th magnet (15), the 8th magnet (16) respectively for cover plate (1) and base plate (2); Magnetic separator frame (6) is inlaid on the cover plate (1) of circulation detecting pool device (19), magnetic separator frame (6) has the first base hole (601), the second base hole (602), the 3rd base hole (603), the 4th base hole (604), the 5th base hole (605), the 6th base hole (606), the 7th base hole (607), the 8th base hole (608), the 9th base hole (609), the tenth base hole (610), the 11 base hole (611), the 12 base hole (612), separates pipe for fixing magnetic.
2. the micro-circulation detecting pool device of quick assembling separated with magnetic according to claim 1 is characterized in that: the angle between described the first pipe jointer (7), second pipe connector (8) and normal is 0 degree ~ 90 degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203157208U CN203310791U (en) | 2013-06-03 | 2013-06-03 | Rapid-assembly micro-circulation detection pool device with magnetic separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203157208U CN203310791U (en) | 2013-06-03 | 2013-06-03 | Rapid-assembly micro-circulation detection pool device with magnetic separation |
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| Publication Number | Publication Date |
|---|---|
| CN203310791U true CN203310791U (en) | 2013-11-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013203157208U Expired - Lifetime CN203310791U (en) | 2013-06-03 | 2013-06-03 | Rapid-assembly micro-circulation detection pool device with magnetic separation |
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| CN (1) | CN203310791U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
-
2013
- 2013-06-03 CN CN2013203157208U patent/CN203310791U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323492A (en) * | 2013-06-03 | 2013-09-25 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
| CN103323492B (en) * | 2013-06-03 | 2015-05-06 | 浙江大学 | Rapid assembly micro-flow detection cell device with magnetic separation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20131127 Effective date of abandoning: 20150506 |
|
| RGAV | Abandon patent right to avoid regrant |