CN203295499U - Novel special stem tip meristem infestation dish for transgenosis - Google Patents
Novel special stem tip meristem infestation dish for transgenosis Download PDFInfo
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- CN203295499U CN203295499U CN201320295177XU CN201320295177U CN203295499U CN 203295499 U CN203295499 U CN 203295499U CN 201320295177X U CN201320295177X U CN 201320295177XU CN 201320295177 U CN201320295177 U CN 201320295177U CN 203295499 U CN203295499 U CN 203295499U
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Abstract
The utility model belongs to the technical field of experimental apparatuses, and particularly relates to a novel special stem tip meristem infestation dish for transgenosis. The dish comprises a dish body and a dish cover, wherein at least one round or square fixing plate is arranged in the dish body; at least one inserting hole is formed in the surface of the fixing plate; and at least one liquid injection hole is formed in the surface of the fixing plate. According to the technical scheme provided by the utility model, one fixing plate provided with the inserting hole is arranged in the dish body and effectively enables corn stem tips to be inversely inserted and fixed into a plate, so that the stem tips are prevented from dispersed, each grain of the material can be sufficiently contacted with an infestation liquid, and the experimental pollution can be effectively controlled; and further, the novel special stem tip meristem infestation dish for transgenosis has the characteristics of simple structure, simplicity and convenience in operation, convenience and practicability, and can be widely used in the stem tip infestation experiment.
Description
Technical field
The utility model belongs to the experiment equipment technical field, specifically relates to the special-purpose shoot apical meristem of a kind of novel transgenosis and infects ware.
Background technology
In the experiment of corn stem apex genetic transformation, with agrobacterium-mediated transformation, foreign gene is imported to plant, this crosses the range request high cleaning, prevents material contamination, avoids affecting the efficiency of genetic transformation.Material in this experiment is the Course of Corn Seed Germination seedling, when the sprouting height of seedling grows to the 3-6cm left and right, on aseptic working platform, peels off coleoptile and spire, exposes shoot tip meristem, with scalpel is slight, dampens for transforming; Material after processing is about the 2-4cm left and right, at first this material shoot tip meristem position towards the orderly pendulum of direction in the plate the inside, follow the principle of lower short upper length, a ware is put 20 left and right of material, can not finalize the design too much, also can not cover the ware lid, can not guarantee the follow-up effect that infects simultaneously.Then plate is put into to moisture eliminator aseptic on super clean bench, porous plastics makes its 30 ° of left and right that tilt on the plate underlay, will infect in the culture dish that liquid adds inclination, infects liquid and does not have stem apex, tries not to be infected with other position.Cover the vacuum pump lid, under 50kPa pressure, infect about 10min, after end with unnecessary bacterium liquid on aseptic filter paper exhaustion seed.
In experimentation, step of converting is used culture dish and vacuum drier in a large number, but due to space constraint, plate is put quantity very little, infect efficiency low, take time and effort, expend again the energy, and plate does not have fixed action, the stem apex of putting is easily at random, in experiment, also can't guarantee fully contact infection liquid of every material; Plate is put and can not with stem and other positions of seed, be separated infecting position in addition, is difficult to control pollute, and adding with the sucking-off amount of bacterium liquid can't well be controlled, and easily pollutes.Therefore need a kind of special use that can substitute common plate of invention to infect ware.
The utility model content
The purpose of this utility model is the problem that exists for above-mentioned technology, provides a kind of and can guarantee that conventional efficient, the special-purpose shoot apical meristem of a kind of novel transgenosis simple to operate, that can effectively prevent material contamination infect ware.
For achieving the above object, the technical solution adopted in the utility model is: the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware, by ware body and ware lid, formed, in described ware body, be provided with at least a circle or square retaining plate, on described retaining plate surface, have at least a sieve aperture, the quantity of sieve aperture can be offered according to experiment, and described retaining plate marginal surface place is provided with a liquid filling hole at least, and liquid filling hole quantity also can be offered according to the experiment demand.
Further: described retaining plate is that the 0.3cm high-temperature resistance plastice is made by thickness.
Further: described sieve aperture is that diameter is the circular port of 0.5cm, and described liquid filling hole is the circular port of direct 1.5cm.
Further: described ware body is supporting chimeric the connection with retaining plate.
Further: described ware body is connected by movable rotary column with the ware lid.
Useful technique effect of the present utility model is: in the technical solution of the utility model, because in the ware body, be provided with a retaining plate with sieve aperture, it can make corn stem apex material be fixed in plate effectively, prevent that stem apex is at random, guarantee fully contact infection liquid of every material, effectively Control experiment pollutes; The utlity model has characteristics simple in structure, easy and simple to handle, convenient and practical, can be widely used in stem apex and infect in experiment.
The accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, below will the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is structural representation of the present utility model.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, rather than whole embodiment.Based on the embodiment in the utility model, those of ordinary skills are not making under the creative work prerequisite the every other embodiment that obtains, and all belong to the scope of the utility model protection.
As shown in Figure 1, the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware, by ware body 1 and ware lid 2, formed, ware body 1 is connected by movable rotary column with ware lid 2, and the folding of infecting ware easy to connect of movable rotary column, can be also obviously other on-link mode (OLM)s, in ware body 1, be provided with the retaining plate 3 of a circle, retaining plate 3 is that the 0.3cm high-temperature resistance plastice is made by thickness, on retaining plate 3 surfaces, has 62 sieve apertures 4, retaining plate 3 Surface Edge along on have two liquid filling holes 5; Obviously the quantity of sieve aperture and liquid filling hole can be other quantity.
Wherein, sieve aperture 4 is that diameter is the circular port of 0.5cm, and liquid filling hole 5 is circular ports of direct 1.5cm.
Wherein, ware body 1 is supporting chimeric the connection with retaining plate 3.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (5)
1. the special-purpose shoot apical meristem of novel transgenosis infects ware, by ware body (1) and ware lid (2), formed, it is characterized in that: in described ware body (1), be provided with at least a circle or square retaining plate (3), described retaining plate (3) has a sieve aperture (4) on surface at least, and described retaining plate (3) has a liquid filling hole (5) on surface at least.
2. the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware according to claim 1, and it is characterized in that: described retaining plate (3) is that the 0.3cm high-temperature resistance plastice is made by thickness.
3. the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware according to claim 1, and it is characterized in that: described sieve aperture (4) is that diameter is the circular port of 0.5cm, and described liquid filling hole (5) is the circular port of direct 1.5cm.
4. the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware according to claim 1, it is characterized in that: described ware body (1) is supporting chimeric the connection with retaining plate (3).
5. the special-purpose shoot apical meristem of a kind of novel transgenosis infects ware according to claim 1, it is characterized in that: described ware body (1) is connected by movable rotary column with ware lid (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320295177XU CN203295499U (en) | 2013-05-27 | 2013-05-27 | Novel special stem tip meristem infestation dish for transgenosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320295177XU CN203295499U (en) | 2013-05-27 | 2013-05-27 | Novel special stem tip meristem infestation dish for transgenosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203295499U true CN203295499U (en) | 2013-11-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320295177XU Expired - Fee Related CN203295499U (en) | 2013-05-27 | 2013-05-27 | Novel special stem tip meristem infestation dish for transgenosis |
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| CN (1) | CN203295499U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923824A (en) * | 2014-04-24 | 2014-07-16 | 山东省农业科学院玉米研究所 | Special culture dish for transgenetically dip-dyeing meristem of stem tip of corn seedling |
-
2013
- 2013-05-27 CN CN201320295177XU patent/CN203295499U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923824A (en) * | 2014-04-24 | 2014-07-16 | 山东省农业科学院玉米研究所 | Special culture dish for transgenetically dip-dyeing meristem of stem tip of corn seedling |
| CN103923824B (en) * | 2014-04-24 | 2015-11-18 | 山东省农业科学院玉米研究所 | A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131120 Termination date: 20140527 |