CN103923824B - A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish - Google Patents
A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish Download PDFInfo
- Publication number
- CN103923824B CN103923824B CN201410167967.9A CN201410167967A CN103923824B CN 103923824 B CN103923824 B CN 103923824B CN 201410167967 A CN201410167967 A CN 201410167967A CN 103923824 B CN103923824 B CN 103923824B
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- CN
- China
- Prior art keywords
- seedling
- jack
- plate
- container
- culture dish
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Abstract
A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish, it is a kind of special tool, it comprises container and carries seedling plate, carrying seedling plate is a stepped waffle slab with multiple not loading end at grade, and multiple uniform jack is all provided with on each described loading end, described jack just holds corn seedling and is inverted insertion, the wound of corn seedling is made substantially to be in same plane, having in the above-described container for supporting the rib carrying seedling plate, a year seedling plate being taken and is placed on rib.It is a kind of special tool, and seedling is upside down in suitable jack by the length according to seedling, and finally the top wound of each seedling is in same plane substantially, is convenient in the bacterium liquid in neat insertion container, and dip-dye position is more accurate.
Description
Technical field
The utility model relates to the container of the dip-dye used in a kind of laboratory, and specifically a kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish.
Background technology
Traditional dip-dye apparatus is glass culture dish, be exactly by the tip of the corn seedling through band girdle and rip cutting down, a small amount of bacterium liquid is placed in culture dish, and culture dish is in heeling condition, make bacterium liquid amass in least significant end, as Figure 11, be inverted cutting through injured seedling, be placed in culture dish, add bacterium liquid and do not have apical position, within about 10 minutes, contaminate vacuumizing in moisture eliminator to vacuumize.At present there are no the special container used for this dip-dye experiment.
Traditional dip-dye experiment Problems existing is, the first, soak position not accurately, and as Figure 11, in traditional culture dish, because the upside down of seedling 4 inclination is in culture dish 8, irregular, and bacterium liquid can be contaminated the root generation of seedling, causes the failure of an experiment; Two is that bacterium liquid is easily trickled down, and because the degree of depth of culture dish is more shallow, and is in heeling condition again, and in the process of transfer culture dish, bacterium liquid is easily trickled down, and pollutes event; 3rd, traditional modes of emplacement, as shown in figure 11, makes the comparatively small amt of the seedling of the placement in culture dish, per unit batch vacuumize in moisture eliminator the seedling can only placing a small amount of tens strains, inefficiency, and the waste causing lab resources.
Utility model content
For the problems referred to above, the utility model provides a kind of corn seedling shoot apical meristem transgenosis to contaminate special culture dish, improve the volume of the seedling of contaminating in experiment in unit space, and can realize accurately to needing the wound location contaminated to contaminate accurately.
The technical scheme in the invention for solving the technical problem is: a kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish, comprises container and carries seedling plate, it is characterized in that,
Described year seedling plate is a stepped waffle slab with multiple not loading end at grade, and multiple uniform jack is all provided with on each described loading end, described jack comprises small one and large one two circular holes, and carry out through between, form a strip hole, wherein the stem of seedling is passed in great circle arc, then seedling is moved to small-bore place, described jack just holds corn seedling and is inverted insertion, and the wound of corn seedling is in same plane substantially
Have in the above-described container for supporting the rib carrying seedling plate.
Further, described container is uncovered rectangular-shaped, and described year seedling plate is Rectangular grid plate, is ladder-like arranged at described loading end.
Further, described container is uncovered barrel-shaped, and described year seedling plate is roundness mess plate, and described loading end is not concentric(al) circles at grade.
Further, described container and a year seedling plate are that stainless steel stamping parts is or/and moulding.
Further, the lid that can be fastened on described container is also comprised.
Further, described in each, jack place embeds a falope ring.
The beneficial effects of the utility model are: be a kind of special tool, seedling is upside down in suitable jack by the length according to seedling, the top wound of each seedling final is in same plane substantially, is convenient in the bacterium liquid in neat insertion container, and dip-dye position is more accurate.
Jack can be distributed on and carry on seedling plate, because the seedling be inserted in jack is in vertical state, the effect than comparatively dense can be formed, about long 20 centimetres at one, wide 10 centimetres box just can place seedling 100 strain, in unit space, the quantity of the seedling of carrying is original several times, improves the utilization ratio of vacuum drier and the efficiency of experiment.
Accompanying drawing explanation
Fig. 1 is the stereographic map of embodiment one.
Fig. 2 is the front view of Fig. 1.
Fig. 3 is A--A sectional view in Fig. 2.
Fig. 4 is the stereographic map of the container in Fig. 1.
Fig. 5 is the stereographic map carrying seedling plate in Fig. 1.
Fig. 6 is the schematic diagram of embodiment one.
Fig. 7 is the schematic diagram that this culture dish is placed in vacuum drier.
Fig. 8 is the overall perspective view of embodiment one.
Fig. 9 is the dismounting schematic diagram of embodiment two.
Figure 10 is the schematic diagram of embodiment two.
Figure 11 is the dip-dye view of original seedling in culture dish.
In figure: 1 container, 11 ribs, 2 years seedling plates, 21 loading ends, 211 first loading ends, 212 second loading ends, 213 the 3rd loading ends, 22 overlapping parts, 23 flange, 3 lids, 4 seedling, 5 jacks, 6 bacterium liquid, 7 vacuumize moisture eliminator, 8 culture dish, 1 ' container, and 2 ' carries seedling plate, 21 ' loading end.
Embodiment
Embodiment one, as shown in Figures 1 to 8,
A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish, comprises container 1, carries seedling plate 2 and lid 3.
As Fig. 5, carrying seedling plate 2 is stainless steel pressed sheet, and on pressed sheet, once synchronous flushing extrudes multiple jack 5, and the pattern of this jack 5 is such, comprises small one and large one two circular holes, and carries out through between, forms a strip hole.The effect of great circle arc is wherein, the stem of seedling 4 is passed in great circle arc, because aperture is comparatively large, be convenient to through, then seedling is moved to small-bore place.Certainly above-mentioned jack also can be the shape such as circular hole, elliptical aperture.A falope ring can also be embedded at jack place, tender seedling stem is protected, prevent from scratching.
Because the length of the stem of seedling 4 differs, the pattern with stepped effect is designed to by carrying seedling plate, three loading ends 21 namely shown in Fig. 5, and be spaced apart 2 centimetres or 3 centimetres between every two loading ends, when placing seedling, the seedling of stem head is placed on the first loading end 211, the shorter seedling of stem is placed on the second loading end 212, the shortest is placed on the 3rd loading end 213, ensure that the wound part of each seedling of inversion state is in same level substantially, effectively can ensure that the significant part of 2 centimetres-3 centimetres of seedling wound is immersed in bacterium liquid 6 completely like this under inversion state.
Be provided with the flange 23 of two bendings at the overlapping part 22 carrying seedling plate 2 both sides, form two and pick up the ears, pin can propose carrying seedling plate with the forefinger of two fingers, conveniently pick and place.
Container 1 is a stainless steel box, and have in the inner side of box and carry the rib 11 of seedling plate for supporting, this rib 11 can be welded and fixed by the mode of soldering stainless steel bar, also can adopt that to carry out overall stamping forming mode at container one-body molded.
Lid 3, lid is stainless steel stamping parts, and this is easy understand, and lid 3 is fastened on container 1, protects.
Using method is as follows, first, be placed in the container 1 with bacterium liquid 6 stand-by by carrying seedling plate 2, corn seedling 4 to be infected is upside down in jack, the jack of suitable loading end is selected according to the length of seedling, the like, until seedling to be covered with whole year seedling plate 2, then whole culture dish is placed on to vacuumize in moisture eliminator 7 and carries out vacuumizing dip-dye ten minutes, namely complete dip-dye work.
Relative to the mode that traditional culture dish tilts, one long 20 centimetres, and about the box of wide 10 centimetres just can place seedling 100 strain, in unit space, the quantity of the seedling of carrying is original several times, and wound is substantially in the same plane, the precision of dip-dye have also been obtained and significantly improves.And structure is the simplest, sterilizes and use all very convenient.
Embodiment two, as shown in Figure 9 and Figure 10,
Be with embodiment one difference, container 1 ' and the shape of carrying seedling plate 2 ' be circular, correspondence be also one to be circular layout at described year each loading end 21 ' of seedling plate, such structure one is convenient to stamped, and two is have good appearance effect.
Easy understand, above-mentioned container, year seedling plate and lid can use the plastic injection piece of food grade to make, and can realize above-mentioned purpose equally.
Except the technical characteristic described in specification sheets, be the known technology of those skilled in the art.
Claims (2)
1. corn seedling shoot apical meristem transgenosis contaminates a special culture dish, comprises container and carries seedling plate, it is characterized in that,
Described year seedling plate is a stepped waffle slab with multiple not loading end at grade, and described year seedling plate is Rectangular grid plate, is ladder-like arranged at described loading end; Each described loading end is all provided with multiple uniform jack, and jack place embeds a falope ring described in each, described jack comprises small one and large one two circular holes, and carry out through between, form a strip hole, wherein said jack holds corn seedling and is inverted insertion, and the stem of seedling is passed in great circle arc, then seedling is moved to small-bore place;
Described container is uncovered rectangular-shaped, has for supporting the rib carrying seedling plate in the above-described container;
Described container and a year seedling plate are stainless steel stamping parts.
2. a kind of corn seedling shoot apical meristem transgenosis according to claim 1 contaminates special culture dish, it is characterized in that, also comprises the lid that can be fastened on described container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410167967.9A CN103923824B (en) | 2014-04-24 | 2014-04-24 | A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410167967.9A CN103923824B (en) | 2014-04-24 | 2014-04-24 | A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103923824A CN103923824A (en) | 2014-07-16 |
| CN103923824B true CN103923824B (en) | 2015-11-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410167967.9A Expired - Fee Related CN103923824B (en) | 2014-04-24 | 2014-04-24 | A kind of corn seedling shoot apical meristem transgenosis contaminates special culture dish |
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| CN (1) | CN103923824B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5274953A (en) * | 1992-07-22 | 1994-01-04 | Plantel Nurseries, Inc. | Planting machine suitable seedling tray |
| CN103362256A (en) * | 2013-08-02 | 2013-10-23 | 徐州大亚自动装备有限公司 | Compound solar vertical hinged skylight |
| CN203295499U (en) * | 2013-05-27 | 2013-11-20 | 四川农业大学 | Novel special stem tip meristem infestation dish for transgenosis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1014343C2 (en) * | 2000-02-10 | 2001-08-13 | Flexivorm Beheer B V | Device and method for forcing flower bulbs. |
| US20120137581A1 (en) * | 2009-08-07 | 2012-06-07 | Nuplant Pty Ltd | Plantlet handling system |
-
2014
- 2014-04-24 CN CN201410167967.9A patent/CN103923824B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5274953A (en) * | 1992-07-22 | 1994-01-04 | Plantel Nurseries, Inc. | Planting machine suitable seedling tray |
| CN203295499U (en) * | 2013-05-27 | 2013-11-20 | 四川农业大学 | Novel special stem tip meristem infestation dish for transgenosis |
| CN103362256A (en) * | 2013-08-02 | 2013-10-23 | 徐州大亚自动装备有限公司 | Compound solar vertical hinged skylight |
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| Publication number | Publication date |
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| CN103923824A (en) | 2014-07-16 |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151118 Termination date: 20190424 |