CN203249807U - Device for absorbing and preserving urine protein - Google Patents
Device for absorbing and preserving urine protein Download PDFInfo
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- CN203249807U CN203249807U CN 201320148076 CN201320148076U CN203249807U CN 203249807 U CN203249807 U CN 203249807U CN 201320148076 CN201320148076 CN 201320148076 CN 201320148076 U CN201320148076 U CN 201320148076U CN 203249807 U CN203249807 U CN 203249807U
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- membrane
- funnel
- filter
- filter paper
- adhesion protein
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Abstract
A device for absorbing and preserving urine protein is characterized by comprising the following key components: a funnel, 1-8 layers of filter paper placed in the funnel and a filtration film which is placed on the filter paper and used for absorbing protein, wherein the filter paper and the filtration film for absorbing protein can guarantee that a liquid poured from the position above the funnel is filtered to the below only through the filter paper and the filtration film for absorbing protein, and the filtration film for absorbing protein is selected from a PVDF (polyvinylidene fluoride) film, a cellulose nitrate membrane, a nylon film and a glass fiber film. The device for absorbing and preserving urine protein is simple in structure and easy to prepare, and can be used for absorbing and preserving urine protein at the room temperature.
Description
Technical field
The utility model relates to the device of absorption, preservation urine albumen.Particularly, the utility model relates to the device of the filter membrane long preservation urine albumen that utilizes adhesion protein.
Background technology
For medical research and medical practice, biological sample all has great value.Yet biological specimen is not comprehensively preserved as case history in the current medical practice.This may be since the collection of biological specimen often have traumatic, biological specimen be kept at technical have difficulty and somewhat expensive.
Urine is a kind of important body fluid, contains the information that patient enriches, and the more important thing is, it is a kind of biological specimen that can gather without wound fully.Urine albumen is considered to contain the potential mark of kidney trouble.In fact, be difficult to find again a kind of biological specimen as urine in the human body, both can be fully without the wound collection, and for example this is near the interior a kind of organ of body and reflect the state of this organ.The more important thing is and since urine be blood plasma at the filtration product of kidney, therefore urinate the information that not only comprises urinary system in the albumen, and comprise the information of whole health.
In sum, urine is a kind of important biological specimen in the body, and along with the progress of the course of disease, the urine albumen of every one-phase all should keep getting off.The clinical sample of protein marker checking after the preservation of a large amount of urine albumen will promote is beneficial to research and the final conversion to clinical practice of biomarker.
At present, the preservation of urine generally is frozen in the refrigerator of-80 ° of C.Because volume of urine is large, protein concentration is low, and the preservation of urine often needs huge volume.More seriously, when urine albumen is kept in the liquid, even if frozen in the environment of-80 ° of C, also than preserve be easy to degrade many under the dry condition.
The filter membrane of adhesion protein refers to have the filter membrane of adhesion protein ability, mainly comprises pvdf membrane, nitrocellulose filter, nylon membrane and glass fibre membrane.
Pvdf membrane is PVDF membrane (polyvinylidene fluoride), has higher physical strength, is a kind of solid support commonly used in the immunoblotting.So far, pvdf membrane is mainly used in dot hybridization, in the protein imprinted experiment.Similarly, also have no the report that other filter membrane is used for urine protein adsorption and preservation.
Therefore, develop a kind of device of preservation urine albumen of cheap and simple, can be used as the beginning of comprehensive preservation biological specimen.The utility model will be urinated on the filter membrane that albumen rapid draing is kept at adhesion protein, and simple environmental protection and economy is for extensive patient's protein biology sample of preserving provides possibility comprehensively.
The utility model content
Therefore, technical purpose of the present utility model is to seek a kind of device of simply long preservation urine albumen.
Therefore, for achieving the above object, the utility model provides the device of a kind of absorption, preservation urine albumen, it is characterized in that it comprises following critical component:
Funnel; Place the 1-8 metafiltration paper in the funnel; Place the filter membrane of the adhesion protein on the filter paper;
Wherein the filter membrane of filter paper and adhesion protein can guarantee the liquid poured into from funnel top only the membrane filtration by filter paper and adhesion protein is to the below, and wherein the filter membrane of adhesion protein is selected from pvdf membrane, nitrocellulose filter, nylon membrane and glass fibre membrane.In preferred embodiment, the filter membrane of described adhesion protein is pvdf membrane.
Size and the material of the funnel that the utility model is used are not particularly limited, as long as the ability of the described filter paper of support and its adhesion protein that can realize is lower than the filter membrane of adhesion protein.In preferred embodiment, described funnel is common glass funnel.The used filter paper of the utility model also is not particularly limited, and the ability that needs only its filter membrane that can support described adhesion protein and adhesion protein is lower than the filter membrane of adhesion protein.
In preferred embodiment, described filter paper is close to the funnel inside surface.Certainly, in order to strengthen the area of filtration, described filter paper can be folded into covering of the fan, and in this case, only part filter paper is adjacent to the funnel inside surface.In preferred embodiment, described filter paper is the 4-6 layer, and the filter membrane of described adhesion protein is individual layer.In preferred embodiment, be coated with the low protein adsorption filter membrane in 0.45 micron in aperture on the filter membrane of described adhesion protein.
In preferred embodiment, described funnel is Buchner funnel.In this case, the filter membrane of described filter paper and adhesion protein places on the wire side of Buchner funnel and the size of the filter membrane of filter paper and adhesion protein guarantees the complete wire side that covers Buchner funnel.In preferred embodiment, described funnel effectively is connected with the vacuum filtration device, accelerates the filter membrane that liquid filters described filter paper and adhesion protein to utilize the formed negative pressure of suction filtration.Perhaps, described funnel top adds a dismountable sealing pressing device, to accelerate the filter membrane that liquid filters described filter paper and adhesion protein by pressurization.
Below in conjunction with said structure of the present utility model its useful technique effect is described: the device of absorption of the present utility model, preservation urine albumen utilize common experiment material convenient, will urinate on the filter membrane that albumen rapid draing is kept at adhesion protein at an easy rate.Device of the present utility model provides possibility for the extensive patient urine protein biology sample of preserving comprehensively.
Description of drawings
Fig. 1: the device 1 that the adsorbing urine liquid eggs is white.
Fig. 2: install 1 part sectioned view.
Fig. 3: the device 2 that the adsorbing urine liquid eggs is white.
Fig. 4: install 2 part sectioned views.
Fig. 5: the device 3 that the adsorbing urine liquid eggs is white.
Fig. 6: install 3 part sectioned views.
Embodiment
Relevant the utility model is for achieving the above object, and the technological means that adopts and effect thereof are now enumerated possible embodiments, and cooperates description of drawings as follows:
At first, see also Fig. 1, it illustrates a kind of simple realization mode of the device of absorption of the present utility model, preservation urine albumen, and wherein 4 metafiltration paper 2 place in the funnel with the form of being close to hopper walls 1, and the filter paper that one deck pvdf membrane 3 is close to the superiors places in the funnel.Described filter paper and pvdf membrane form the similar shape of shape with funnel, and preferably, in the situation that filter paper and pvdf membrane size are certain, make the formed space of filter paper and pvdf membrane maximum, to hold more urine as far as possible as far as possible.The utility model there is no specific (special) requirements for indexs such as the thickness of filter paper, granularity, flow velocitys, supports pvdf membrane, makes pvdf membrane be close on it and filtered solution is flow through rapidly as long as be beneficial to, and preferably, described filter paper is used water infiltration before use.Equally, the utility model, attaches filter paper on it, pvdf membrane and the filtered solution that forms is imported bottom container as long as can satisfy stable support also without being strict with for the shape of funnel, material, size.The used pvdf membrane of the utility model is exactly the most frequently used pvdf membrane of biological field, there is no specific (special) requirements.Described pvdf membrane immerse successively before use activate in the methyl alcohol with pure water in clean, and to avoid the generation of bubbling when placing on the filter paper as far as possible.Although the many adsorbing urine liquid eggs that can be as much as possible of the pvdf membrane number of plies are white in theory, the utility model finds, the pvdf membrane of individual layer both can adsorb enough urine albumen, also can guarantee the speed that filters.In the present embodiment, the urine of pouring in the funnel filters described pvdf membrane and filter paper by Action of Gravity Field.Fig. 2 has shown the part sectioned view of device shown in Figure 1, is specially the sectional view of funnel skew wall.
Fig. 3 has shown a kind of improved implementation of the device of absorption of the present utility model, preservation urine albumen, unique difference of itself and device shown in Figure 1 is, also be glued with the low protein adsorption filter membrane 4 in 0.45 micron in aperture on described pvdf membrane, this filter membrane is used for removing cell and the cell fragment of urine.Fig. 4 has shown the part sectioned view of device shown in Figure 3, is specially the sectional view of funnel skew wall.
Fig. 5 has shown a kind of better implementation of the device of absorption of the present utility model, preservation urine albumen, wherein Buchner funnel 1 effectively is connected with vacuum filtration device 2, wire side 3 at Buchner funnel 1 is placed with 6 metafiltration paper 4, is glued with pvdf membrane 5 at filter paper 4.Before use, preferably make filter paper 4 use water infiltration, described pvdf membrane immerse successively before use activate in the methyl alcohol with pure water in clean, and to avoid the generation of bubble when placing on the filter paper as far as possible.The shape of described filter paper and pvdf membrane can be circular, also can be other shapes, and unique requirement is that it will intactly cover the mesh on the Buchner funnel wire side, so that urine only can filter via the filtering layer that described pvdf membrane and filter paper form.During use, urine is poured in the funnel, opened the vacuum filtration device that effectively is connected with funnel, rely on the urine in the negative pressure Accelerating funnel that produces to filter described PDVF film and filter paper.Fig. 6 has shown the part sectioned view of device shown in Figure 5, is specially the sectional view of Buchner funnel wire side.
Certainly, on the pvdf membrane 5 of device shown in Figure 5, also can be close to again the low protein adsorption filter membrane 6 in 0.45 micron in an aperture, be used for removing cell and the cell fragment of urine.
The vacuum filtration device of device shown in Figure 5 also can replace 7 by the dismountable sealing pressing device that places Buchner funnel top, and in this case, the urine in the funnel will utilize the added pressure in top to accelerate to filter described pvdf membrane and filter paper.
Claims (10)
1. the device of an absorption, preservation urine albumen is characterized in that it comprises following critical component:
Funnel; Place the 1-8 metafiltration paper in the funnel; Place the filter membrane of the adhesion protein on the filter paper;
Wherein the filter membrane of filter paper and adhesion protein can guarantee the liquid poured into from funnel top only the membrane filtration by filter paper and adhesion protein is to the below, and wherein the filter membrane of adhesion protein is selected from pvdf membrane, nitrocellulose filter, nylon membrane and glass fibre membrane.
2. the device of absorption according to claim 1, preservation urine albumen, the filter membrane that it is characterized in that described adhesion protein is pvdf membrane.
3. the device of absorption according to claim 1, preservation urine albumen is characterized in that described filter paper is close to the funnel inside surface.
4. the device of absorption according to claim 1, preservation urine albumen is characterized in that described filter paper is the 4-6 layer.
5. the device of absorption according to claim 1, preservation urine albumen, the filter membrane that it is characterized in that described adhesion protein is individual layer.
6. the device of absorption according to claim 1, preservation urine albumen is characterized in that being coated with on the filter membrane of described adhesion protein the low protein adsorption filter membrane in 0.45 micron in aperture.
7. the device of absorption according to claim 1, preservation urine albumen is characterized in that described funnel is Buchner funnel.
8. the device of absorption according to claim 7, preservation urine albumen, the filter membrane that it is characterized in that described filter paper and adhesion protein place on the wire side of Buchner funnel and the size of the filter membrane of filter paper and adhesion protein guarantees the complete wire side that covers Buchner funnel.
9. according to claim 7 or the device of 8 described absorption, preservation urine albumen, it is characterized in that described funnel effectively is connected with the vacuum filtration device, accelerate the filter membrane that liquid filters described filter paper and adhesion protein to utilize the formed negative pressure of suction filtration.
10. according to claim 7 or the device of 8 described absorption, preservation urine albumen, it is characterized in that described funnel top adds a dismountable sealing pressing device, to accelerate the filter membrane that liquid filters described filter paper and adhesion protein by pressurization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320148076 CN203249807U (en) | 2013-03-28 | 2013-03-28 | Device for absorbing and preserving urine protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320148076 CN203249807U (en) | 2013-03-28 | 2013-03-28 | Device for absorbing and preserving urine protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203249807U true CN203249807U (en) | 2013-10-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320148076 Expired - Lifetime CN203249807U (en) | 2013-03-28 | 2013-03-28 | Device for absorbing and preserving urine protein |
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| CN (1) | CN203249807U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567670A (en) * | 2014-10-11 | 2016-05-11 | 中国医学科学院基础医学研究所 | Method and apparatus for preserving nucleic acid in urine sample |
| CN108344620A (en) * | 2017-01-23 | 2018-07-31 | 北京师范大学 | A kind of method and its device concentrated and preserve genomic DNA in urine specimen |
-
2013
- 2013-03-28 CN CN 201320148076 patent/CN203249807U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567670A (en) * | 2014-10-11 | 2016-05-11 | 中国医学科学院基础医学研究所 | Method and apparatus for preserving nucleic acid in urine sample |
| CN108344620A (en) * | 2017-01-23 | 2018-07-31 | 北京师范大学 | A kind of method and its device concentrated and preserve genomic DNA in urine specimen |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131023 |
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| CX01 | Expiry of patent term |