CN203244989U - Device used for performing parallel operation of mixing and extracting multiple liquid samples - Google Patents
Device used for performing parallel operation of mixing and extracting multiple liquid samples Download PDFInfo
- Publication number
- CN203244989U CN203244989U CN2013201243957U CN201320124395U CN203244989U CN 203244989 U CN203244989 U CN 203244989U CN 2013201243957 U CN2013201243957 U CN 2013201243957U CN 201320124395 U CN201320124395 U CN 201320124395U CN 203244989 U CN203244989 U CN 203244989U
- Authority
- CN
- China
- Prior art keywords
- test tube
- distal portions
- proximal part
- liquid
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0851—Bottom walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
Abstract
The utility model discloses a device used for performing parallel operation of mixing and extracting multiple liquid samples. The device comprises a multi-position operation platform, wherein each position accommodates a test tube and further comprises a physicochemical condition control part and a switch element; each test tube comprises an opening allowing a liquid to be added and sucked, a proximal end part adjacent to the opening, as well as a remote end part allowing the liquid to be mixed and perform a physicochemical reaction; and each proximal end part is provided with a clamping part which can be repeatedly converted between the extrusion closing state and the looseness opening state. The test tubes and the device can conveniently and effectively solve the cross contamination problem during the liquid mixing and extracting process, and contribute to high-throughput and automatic operation of a liquid mixing and extracting process; and the device is particularly suitable for separating and detecting target substances from biological samples, such as nucleic acid.
Description
The utility model is that application number is 201190000533.X, and denomination of invention is divided an application for the utility model of " test tube, the method that comprises the device of this test tube and utilize their treat liquid ".
Technical field
The utility model relates to a kind of for the test tube that liquid mixes and extraction operates, and described test tube is specially adapted to separation and detection target substance, for example nucleic acid from biological sample.The utility model further relates to a kind of device for a plurality of fluid samples being mixed and extract operation repetitive, and described device comprises described test tube.In addition, the utility model also provides and has used described test tube and device liquid to be mixed and extracts the method for operation.
Background technology
In many experimental sciences field, in the fields such as chemistry, biology, medical science, all relate to mixing and extraction process to liquid.For example, in chemical field, quite a few chemical reaction all carries out in liquid and finishes.And in biology and medical domain, a large amount of biological analysis detections all needs at first to separate and be purified into labelled molecule with medical diagnosis from biological specimen, for example from cell, isolate characteristic protein and nucleic acid, and described separation and purification step carry out in liquid environment usually also.Liquid mix and the various techniques extracted particularly in Multi-example operation repetitive process, owing to there are a series of continuous, loaded down with trivial details operating procedures, such as liquid feeding, move liquid, vibration mixes, regulates physical and chemical parameter (such as temperature, pH) etc., careless slightlyly namely may cause particularly volatile liquid generation cross pollution of fluid sample.Cross-contamination issue also so has greatly increased the difficulty that liquid mixes and extraction process realization high flux, automation are controlled.
Take separate nucleic acid and purifying process as example.Most of separate nucleic acid all comprise lysis, enzyme processing, thereby nucleic acid are separated several key steps such as purification of nucleic acid with the other biological macromolecular substances with purification process.Each step can be realized alone or in combination by multiple diverse ways again.The high electric charge phosphoric acid skeleton of nucleic acid makes it have more hydrophily than other biological macromolecular substances such as protein, polysaccharide, fat, according to the difference of their physicochemical properties, and can be with separate nucleic acid, purifying with methods such as selective precipitation, chromatography, density gradient centrifugations.Main several method for commercial applications is: centrifugal process, membrane separation process, based on paramagnetic particle method of nanoparticle etc. (referring to for example Molecular Cloning:A Laboratory Manual, the 3rd edition, Cold Spring Harbor Laborotary etc., its content is by the complete this paper that incorporates into).
The characteristic feature of centrifugal process is through separating with centrifuge behind the high-temperature boiling, by getting supernatant and going the way of supernatant to carry out nucleic acid and other macromolecular substances and other impurity and analyze.Its shortcoming is to be difficult to carry out automation mechanized operation, and the process of for example get supernatant, abandoning supernatant is difficult to realize with the mode of automation.Other shortcoming is complex operation, wastes time and energy.The technical staff of a skilled operation also can only process tens samples a morning, and operating personnel need the equipment such as frequent operation metal, centrifuge, and operating personnel's whole process is exposed among the biological pollution.
Membrane separation process often is applied to the fields such as scientific research take offshore companies such as AXYGEN, Qiagen as representative.Generally take scientific research research as main, cost is very high, complex operation.
Paramagnetic particle method based on nanoparticle obtains using more and more widely in recent years.In paramagnetic particle method, after passing through the cell pyrolysis liquid cell lysis, free nucleic acid molecules is out caught specifically to the magnetic-particle surface from cell, and the impurity such as protein are not hunted down and stay in the solution.Subsequently, under magnetic fields, magnetic-particle and liquid are separated, reclaim the magnetic-particle of having caught nucleic acid.After this, thus available eluent obtains purified nucleic acid with nucleic acid wash-out from the magnetic-particle.Because that paramagnetic particle method does not need is centrifugal, do not need to add plurality of reagents, simple to operate, meets the nucleic acid automation and extracts requirement, therefore becomes an important development direction of nucleic acid purification method.
Yet, how effectively to overcome cross pollution and be all the time and needing in nucleic acid extraction high flux, the automation mechanized operation process to realize a major issue solving.Because the high sensitivity of nucleic acid detection assay method, any cross pollution that occurs in the process of purification of nucleic acid sample all probably cause false positive results occurring in follow-up detection is analyzed.And on the other hand, as mentioned above, in series of steps such as heating, vibration, application of sample, washing, the micro liquid that comprises nucleic acid very easily is diffused in the air by aerocolloidal mode, causes the cross pollution between the sample.The known means that overcome cross pollution mainly contain following two kinds at present:
1) cap seal method, namely by use container that lid will be equipped with nucleic acid samples for example test tube seal, only when needs liquid feeding or drawing liquid, lid is opened, thereby has been avoided to greatest extent aerocolloidal diffusion.Yet the shortcoming of this kind method is, in the nucleic acid extraction purge process, and the compound action of the lid that need to repeatedly repeat to uncap/close, this is undoubtedly for realizing that the machine automation mechanized operation has caused great obstacle;
2) oil sealing method namely prevents aerocolloidal diffusion by the liquid level that seals the biological sample that contains nucleic acid with oil reservoir, and stretches into by sample loading gun and to carry out liquid feeding or drawing liquid below the oil reservoir.Yet this kind method has limitation in the process of paramagnetic particle method extraction nucleic acid, because when shifting magnetic-particle through oil reservoir, magnetic-particle is easy to be adsorbed by oil reservoir and be trapped in the oil reservoir, thereby loses.
For above-mentioned reasons, the automaticity of nucleic acid extraction purifying process is also lower at present, also only has two or three enterprise such as Gen-Probe, Roche that nucleic acid extraction and the detection system of the automation of FDA approval are arranged in the world wide.Therefore, be to improve high flux, the automaticity of nucleic acid extraction purifying, need badly exploitation new, effectively and easily overcome the means of cross pollution.
The device that the utility model aims to provide a kind of New test tube and uses this New test tube with overcome easily and effectively liquid mix and leaching process in cross-contamination issue, thereby overcome the deficiency of prior art existence.The application of this New test tube can realize high flux, the automation mechanized operation of liquid technique for extracting mixed easily.
The utility model content
The utility model provides a kind of device that can be used for a plurality of fluid samples are mixed and extract operation repetitive, this device comprises a multiposition operating platform, each position of described operating platform holds a test tube, each position of described operating platform also comprises a physico chemical factor control assembly and a switch element, wherein:
Described test tube comprises can be for adding and the proximal part of the opening of imbitition, adjacent openings and can mix for liquid and carry out the distal portions of physical and chemical reaction, and this proximal part is provided with repeatedly to be closed and to relax in extruding and opens the clamping part of changing between the two states;
The distal portions of the contact of described physico chemical factor control assembly or contiguous described test tube, and can control the mixing of the fluid sample of described distal portions and the physico chemical factor of reaction; And
The proximal part of described switch element contact or contiguous described test tube, and can repeatedly push the clamping part of described proximal part to close and relax and open the conversion and control of two states.
In some embodiments, described physico chemical factor control includes but not limited to the control to the one or more condition in temperature, frequency of oscillation and the magnetic field intensity of the liquid of described distal portions, and described physico chemical factor control assembly is temperature-adjusting device, oscillator and magnet steel.
In some embodiments, described switch element is anchor clamps, is clamped in the clamping part of described proximal part.
In one embodiment, the physico chemical factor control assembly of described a plurality of positions and switch element are integrated interlock, can carry out simultaneously operation repetitive to a plurality of test tubes.
In embodiment further, described multiposition operating platform is a porous plate, each pore volume is received a described test tube, and a plurality of described switch elements are comprised the flat board of porous by unitary construction for another, each borehole jack of described flat board is at the proximal part of each test tube, the folding of the porous on the described flat board is controlled by an elastic device, thereby repeatedly makes the porous on this flat board open or close simultaneously proximal part to a plurality of test tubes and push to close and relax and open the conversion and control of two states simultaneously by this elastic device being applied or removes external force.
More excellent, described porous plate below connects one for the support of fixing and support described test tube.
More excellent, the distal portions of described test tube is made by hard material, and described distal portions comprises bottom and sidewall, thereby the distance of bottom and partial sidewall and angle become a long inclined-plane greater than distance and the angle of itself and other partial sidewall.
In one embodiment, the device that provides of the utility model is used to isolate target components from fluid sample.In further embodiment, described fluid sample comprises target components to be separated and can catch the magnetic-particle of described target components, the outer wall of the distal portions of described test tube is pasted with the magnet steel that can be withdrawn, when described magnet steel is attached to the outer wall of described distal portions, described magnetic-particle is adsorbed, when described magnet steel was withdrawn, described magnetic-particle scattered.Preferably, the distal portions of described test tube is made by hard material, and described distal portions comprises bottom and sidewall, thereby the distance of wherein said bottom and partial sidewall and angle become a long inclined-plane greater than distance and the angle of itself and other partial sidewall, and the described magnet steel that can be withdrawn is attached on the outer wall on described inclined-plane.More preferably, described inclined-plane forms the settling zone at a lower end and sidewall.
Not necessarily, but the device that the utility model provides is inclusion test equipment also, described checkout equipment can carry out various required detections to fluid sample, for example, isolated target components from fluid sample is carried out various signs, include but not limited to determine the composition, structure, content of described target components etc.
Adopt above technical scheme, because the unique design of test tube of the present utility model, the proximal part that can make easily test tube is closed and lax opening between the two states repeatedly changes in extruding, therefore is specially adapted to a plurality of fluid samples are mixed and extract the device of operation repetitive; The cross-contamination issue of liquid in mixing and leaching process be can overcome easily and effectively, high flux, the automation mechanized operation of liquid technique for extracting mixed helped to realize.The utility model is specially adapted to separation and detection target substance, for example nucleic acid from biological sample.
Description of drawings
For ease of understanding the utility model, provide following accompanying drawing.In the application hereinafter, in connection with described accompanying drawing specific embodiments of the present utility model is set forth.Yet, should be appreciated that described accompanying drawing and specific embodiments only for exemplary, it should not consist of any restriction to technical solutions of the utility model.
The structural representation of a certain specific embodiments of the test tube that Fig. 1 provides for the utility model.
Fig. 2 is the structural representation by using anchor clamps that the proximal part extruding of test tube is closed.
Fig. 3 a-3c is for having shown from different perspectives the schematic diagram of the porous plate that comprises the test tube that a plurality of the utility model provide.
The partial schematic diagram of the multiposition operating platform that Fig. 4 a-4b provides for the utility model, wherein in Fig. 4 a, the proximal part of test tube is in the state that extruding is closed, and in Fig. 4 b, the proximal part of test tube is in the lax state that opens.
Fig. 5～Figure 12 shown from fluid sample to separate, to wash and the schematic diagram of the component that diverts the aim, wherein:
Fig. 5 has shown that the proximal part at test tube is in the reagent that is used for extracting target components under the lax state that opens to the test tube adding, comprise the magnetic-particle that can catch middle target components in this reagent, and the proximal part of test tube is closed in subsequently extruding;
Fig. 6 has shown that the proximal part at test tube is in the sample that comprises target components under the lax state that opens to the test tube adding, and the proximal part of test tube is closed in subsequently extruding;
Fig. 7 has shown at the proximal part that makes test tube and has kept pushing under the state of closing that mixing is in reagent and the sample of test tube distal portions and regulates its physico chemical factor, and target components is caught by magnetic-particle thus;
Fig. 8 has shown at the proximal part that makes test tube and has kept pushing under the state of closing that magnetic-particle is adsorbed on the inclined-plane by magnet steel;
Fig. 9 has shown that the proximal part at test tube is under the lax state that opens, from the settling zone sucking-off raffinate of test tube;
Figure 10 has shown the washing to the magnetic-particle of having caught target components.
Figure 11 has shown that external straw stretches to an inclined-plane higher end liquid feeding relative with the settling zone of test tube bottom and the step of mixing.
Figure 12 has shown the step that migrates out target components from the settling zone of test tube bottom.
The specific embodiment
Referring to Fig. 1 and 2, the utility model provides a kind of test tube (10) that can be used for liquid is mixed and extracts operation, and this test tube comprises can be for adding and the proximal part (13) of the opening (12) of imbitition, adjacent openings and can mix for liquid and carry out the distal portions (14) of physical and chemical reaction.The same with conventional test tube, the test tube that the utility model provides has the cavity (11) with opening (12) UNICOM.Different from other test tube known in the art is, the proximal part of test tube of the present utility model (13) can repeatedly be closed and relax to open between the two states in extruding and be replaced, it prevents from being present in liquid spill or the evaporation of distal portions (14) when the extruding closed condition, it allows external straw to stretch to distal portions (14) adding or imbitition by opening when lax open configuration.
Can use the proximal part (13) of multiple means realization the utility model test tube to close and lax repetition conversion of opening between the two states in extruding.For example, the proximal part of test tube (13) can be the flexible pipe of being made by elastomeric material, by using the state of outer clamp (20) control flexible pipe.When the clamping part (29) on anchor clamps (20) grip both hose, described flexible pipe is in the extruding closed condition, thereby seals described test tube; And when the clamping part (29) of anchor clamps (20) from the flexible pipe when unclamping, described flexible pipe is in lax open configuration, thereby opens described test tube.Again for example, the proximal part of test tube (13) can be the flexible pipe of being made by elastomeric material, by using the state of external force control flexible pipe.When without External Force Acting on flexible pipe the time, flexible pipe is in the natural shrinking closed condition.When having External Force Acting on flexible pipe, flexible pipe is in the state that pulled open, thereby opens described test tube.This elastomeric material can use any elastomeric material known in the art to make flexible pipe, as long as can respond external force generation deformation and can restore to the original state after external force is removed.Preferably, can use the elastomeric material that comprises silica gel.
The distal portions of the utility model test tube (14) mixes therein for liquid and physical and chemical reaction occurs, so those skilled in the art can use any suitable material to make any suitable shape as required.A preferred embodiment is to use hard material to make shape hard tube as shown in Figure 1.Described hard tube has bottom and sidewall, thereby the distance of wherein said bottom and partial sidewall and angle become a long inclined-plane (15) greater than distance and the angle of itself and other partial sidewall, and described inclined-plane forms settling zone (16) at a lower end and sidewall.Described bottom shape with inclined-plane (15) and settling zone (16) mixes and extraction at liquid with respect to other shapes, may be particularly advantageous in the technique of paramagnetic particle method extraction target components (for example nucleic acid) particularly.Shown in Fig. 8 and 9, in the paramagnetic particle method extraction process, magnetic-particle (24) can be adsorbed on the inclined-plane (15) by magnet steel (25), the settling zone (16) that external straw (26) can be stretched to the test tube bottom easily exhausts raffinate and other materials, easily avoid magnetic-particle (24), thereby effectively avoid mistake to inhale magnetic-particle (24).On the other hand, as shown in figure 11, when in test tube, adding liquid, external straw is stretched to a described inclined-plane higher end relative with described settling zone, thus, magnetic-particle (24) washes away along inclined-plane (15) in settling zone (16) at the liquid that is added into, and is dispersed in fully in the described liquid, so that magnetic-particle (24) is easier to and liquid blending.Can use any hard material known in the art to make described hard tube, preferably, use PP polypropylene material.
Can make respectively proximal part and the distal portions of test tube of the present utility model, afterwards described part is tightly connected.For example, can use silica gel material to make elastic hose and use the PP polypropylene material to make hard tube, subsequently elastic hose is enclosed within on the hard tube, form complete test tube.Also can proximal part and the distal portions of test tube of the present utility model is integrally formed.According to the disclosure of the specification, those skilled in the art will have the ability to use the ordinary skill in the art to produce test tube of the present utility model fully.
As mentioned above, because the unique design of test tube of the present utility model, the proximal part that can make easily test tube is closed and lax opening between the two states repeatedly changes in extruding, therefore is specially adapted to a plurality of fluid samples are mixed and extract the device of operation repetitive.
Therefore the utility model also provides a kind of device that can be used for a plurality of fluid samples are mixed and extract operation repetitive, and this device comprises a multiposition operating platform, and each position of described operating platform can hold a test tube of the present utility model.
Described multiposition operating platform can comprise a porous plate.For example, as shown in Fig. 3 a-3c, the test tube that a plurality of the utility model provide can be combined in the porous plate (17).Porous plate (17) is provided with a plurality of through holes (18), and a test tube of the present utility model (10) is housed respectively in each through hole (18).Not necessarily, porous plate (17) below has also connected support (19), and test tube of the present utility model (10) can be fixed and support to this support (19) better.The utility model can use various porous plates, comprises being but being not limited to have 12 holes or still less hole, 24 holes, 48 holes, 96 holes, 192 holes, 384 holes or the more porous plate of porous.
In addition, each position of described multiposition operating platform also comprises a switch element, the proximal part of described switch element contact or contiguous described test tube, and can repeatedly push the replacement control of closing and relaxing and opening two states to described proximal part.Described switch element can be anchor clamps (20) as shown in Figure 2, when the proximal part of anchor clamps (20) clamping test tube makes it be in the extruding closed condition, seals described test tube; And unclamp when making it be in lax open configuration from proximal part when anchor clamps (20), open described test tube.
More preferably, the switch element of a plurality of positions can be fabricated as a whole, simultaneously a plurality of test tubes is pushed the replacement control of closing and relaxing and opening two states.As example, show among Fig. 4 that the switch element of a plurality of positions is become another flat board that comprises porous by unitary construction, the proximal part (13) of test tube (10) passes the hole on the described flat board, and the folding in described hole can be controlled by an elastic device.When by for example driving handle (30) when elastic device is applied external force, can close simultaneously the porous on the flat board, thereby so that the proximal part (13) of a plurality of test tube (10) is in the extruding closed condition, and when removing external force, open simultaneously the porous on the flat board, so that the proximal part (13) of a plurality of test tube (10) is in lax open configuration.Perhaps, can apply external force to this elastic device by pulling handle (30), thereby open simultaneously the porous on the flat board, so that the proximal part (13) of a plurality of test tubes (10) is in lax open configuration, and when removing external force, close simultaneously the porous on the flat board, so that the proximal part (13) of a plurality of test tube (10) is in the extruding closed condition.The elastic device of the folding that can realize the hole of the known various routines of those skilled in the art also can be applied in it in switch element of the present utility model.
Each position of operating platform described in the utility model also comprises a physico chemical factor control assembly, the distal portions of its contact or contiguous described test tube, thus can control the mixing of the fluid sample of described distal portions and the physico chemical factor of reaction.Those skilled in the art can according to the needs of concrete technology, design and arrange different physics and chemistry control assemblies.For example by being set, realizes being positioned at the abundant mixing of invisible spectro liquid by oscillator, by the set temperature adjusting device for example water-bath the temperature that is positioned at invisible spectro liquid is regulated, perhaps by change the magnetic field etc. of test tube distal portions with magnet steel in test tube bottom.In certain embodiments, can realize by this area routine techniques the parallel running of the physics and chemistry control assembly of a plurality of positions, thereby the physico chemical factor of the liquid in a plurality of test tubes is carried out identical adjusting simultaneously.
Not necessarily, the device that the utility model provides also can comprise a checkout equipment, and described checkout equipment can carry out various required detections to fluid sample.For example, the device that provides when the utility model is used to isolate the target components for example when protein or nucleic acid that comes from biological cell from biological sample, described checkout equipment can carry out various signs to the target components after separating, and includes but not limited to determine composition, structure, content of described target components etc.In one embodiment, thus described checkout equipment is set to contact or the distal portions of contiguous described test tube need not to shift invisible spectro sample and directly it is detected.In another embodiment, described checkout equipment is comprised in the device that the utility model provides as standalone module, by fluid sample is transferred to described checkout equipment from test tube after sample is detected.
Further, the utility model provides the test tube that uses the utility model to provide liquid to be mixed and extract the method for operation.Preferably, in the method that the utility model provides, thereby the proximal part of the test tube that the utility model provides is only opened test tube adding liquid to the distal portions of this test tube or be in lax open configuration during from imbitition wherein, and at other constantly, for example heat, vibrate, when leaving standstill, the proximal part of the test tube that the utility model provides all is in thus closed test tube of extruding closed condition, be subject to outside contamination thereby reduced most possibly sample, the chance of cross pollution particularly occurs between the sample.
As previously mentioned, method of the present utility model is applicable to the various techniques that need to mix, extract or occur to liquid physical and chemical reaction.Particularly preferably, method of the present utility model is applicable to isolate the target components that comes from biological cell from biological sample, and described target components includes but not limited to protein or nucleic acid.Particularly preferably, described target components is nucleic acid.Therefore, below will take the separate nucleic acid purifying process as example illustrates method of the present utility model.Yet, it will be understood by those skilled in the art that in the mixing or extraction process that method of the present utility model can similarly be applicable to other any liquid is carried out as required.
As an example, Fig. 5-Figure 10 provides the technique of isolating nucleic acid from biological sample, and it comprises following steps:
As shown in Figure 5, when the proximal part (13) of test tube (10) is in lax open configuration, use external straw (23) in test tube (10), to add nucleic acid extraction liquid (21), comprise in the described nucleic acid extraction liquid (21) can specificity target acquisition nucleic acid magnetic-particle, make subsequently described proximal part (13) be in the extruding closed condition.The method of known each the species specificity target acquisition nucleic acid of the utility model.For example, can with can with target nucleic acid on particular sequence realize that the oligonucleotide chain of specific hybrid is covalently bound on the magnetic-particle surface, by the hybridization between described oligonucleotide chain and the target nucleic acid target nucleic acid is captured in the magnetic-particle surface.
Subsequently, as shown in Figure 6, again make the proximal part (13) of test tube (10) be in lax open configuration, use external straw to add sample (22) in test tube (10), described sample contains target nucleic acid to be separated.After finishing application of sample, make described proximal part (13) again be in the extruding closed condition.
Should be appreciated that nucleic acid extraction liquid and sample can be with any order or simultaneously addings.
After this, as shown in Figure 7, keep the proximal part (13) of test tube (10) to be in the extruding closed condition, tube shaken makes the abundant mixing of nucleic acid extraction liquid (21) and sample (22).Subsequently, to the distal portions (14) of test tube (10) thus the biological cell that carries out in the heating pyrolyze sample discharges nucleic acid.For example, can and keep 8 minutes with cell lysis by heating water bath sample to 60 ℃.Finish after the cracking, magnetic-particle is at the lower capture nucleic acid of room temperature (for example 25 ℃).
In next step, as shown in Figure 8, continue to keep the proximal part (13) of test tube (10) to be in the extruding closed condition, magnet steel (25) is attached to test tube (10) thus outer wall on magnetic-particle (24) in the absorption test tube (10).Preferably, magnet steel (25) is attached on the inclined-plane, bottom (15) of test tube (10).Magnetic-particle (24) can be Ferrite Material, and magnet steel (25) can be natural magnet.
Subsequently, make the proximal part (13) of test tube (10) be in lax open configuration, use external straw (26) from test tube (10), preferably the settling zone (16) from test tube (10) bottom exhausts raffinate.
Via above-mentioned steps, target nucleic acid is trapped on the magnetic-particle, fully separates with other components in nucleic acid reaction liquid and the sample, thereby has obtained purified target nucleic acid.
As required, can the target nucleic acid that obtain further be washed.As shown in figure 10, withdraw magnet steel (25), and make the proximal part (13) of test tube (10) be in lax open configuration, in test tube (10), add cleaning solution (27); Subsequently, make the proximal part (13) of test tube (10) be in the extruding closed condition, behind vibration mixing cleaning solution (27) and the magnetic-particle (24), the outer wall that again magnet steel (25) is attached to test tube (10) for example test tube (10) inclined-plane, bottom (15) thus the interior magnetic-particle (24) of upper absorption test tube (10); At last, make the proximal part (13) of test tube (10) be in lax open configuration, use external straw (26) from test tube (10), preferably from the settling zone (16) of test tube (10) bottom cleaning solution is absorbed.Described washing step can be repeated repeatedly as required.
According to the purpose of separation and purification nucleic acid or albumen, can further operate purified nucleic acid or albumen, for example nucleic acid or albumen after separating are detected.The method of various detection nucleic acid known in the art or albumen, include but not limited to, chemoluminescence method (for example, can be referring to U.S. Patent number 7,169,554, it is in full by the complete this paper that incorporates into), the real-time fluorescence method (for example, can be referring to U.S. Patent Application Publication No. US2010/031152A1, wherein disclose by real-time nucleic acid amplification nucleic acid has been carried out quantitative method and system, described patent application is by the complete this paper that incorporates into), and multiple detection method based on antigen-antibody reaction is (such as ELISA, referring to for example Chinese patent application publication number CN1580772A, it is in full by the complete this paper that incorporates into) etc., those skilled in the art can select suitable detection method known in the art according to testing goal.Described detection can directly be carried out in test tube.For example, can in the distal portions of test tube (10), add the required various reaction reagents of detection, and outside described distal portions configuration detection equipment, for example fluorescence excitation device and reading plotter or spectrophotometric readout instrument need not the overall process that transfer nucleic acid namely can be finished extraction, purifying, detection thus.Described detection also can by the nucleic acid behind the purifying is transferred to other checkout gears, for example be carried out in the micro-reaction plate (28) as shown in figure 12, described micro-reaction plate (28) can but to be not limited to be 96 orifice plates.
Above only take single test tube as example, the treatment step to single fluid sample has been described.Those skilled in the art understand fully, and the device that is used for a plurality of fluid samples are mixed and extract operation repetitive by using the utility model to provide can carry out operation repetitive to a plurality of fluid samples in a plurality of test tubes simultaneously.Therefore, the utility model has also been contained a plurality of fluid samples has been mixed and the method for the operation repetitive that extracts simultaneously.As mentioned above, physico chemical factor control assembly and the switch element of each position of the multiposition operating platform that comprises by parallel running device of the present utility model, can be simultaneously opening or the closed state of a plurality of test tubes be carried out parallel control, and the physico chemical factor of wherein fluid sample is carried out synchronous adjusting control.
Should be appreciated that the just purpose in order to illustrate and to be convenient to understand of embodiment that the application describes and specific embodiments, and any restriction is not occured scope of the present utility model.In the situation that does not deviate from the utility model spirit, those skilled in the art can make various modifications, replacement, omission and increase.Scope of the present utility model is only limited by described claim and equivalent way thereof.
Claims (10)
1. device that is used for a plurality of fluid samples are mixed and extract operation repetitive, this device comprises a multiposition operating platform, each position of described operating platform holds a test tube, it is characterized in that, each position of described operating platform also comprises a physico chemical factor control assembly and a switch element, wherein:
Described test tube comprises can be for adding and the proximal part of the opening of imbitition, adjacent openings and can mix for liquid and carry out the distal portions of physical and chemical reaction, and this proximal part is provided with repeatedly to be closed and to relax in extruding and opens the clamping part of changing between the two states;
The distal portions of the contact of described physico chemical factor control assembly or contiguous described test tube, and can control the mixing of the fluid sample of described distal portions and the physico chemical factor of reaction; And
The proximal part of described switch element contact or contiguous described test tube, and can repeatedly push the clamping part of described proximal part to close and relax and open the conversion and control of two states.
2. device as claimed in claim 1 is characterized in that: temperature-adjusting device, oscillator and the magnet steel of described physico chemical factor control assembly for the one or more condition in temperature, frequency of oscillation and the magnetic field intensity of the liquid of described distal portions is controlled.
3. device as claimed in claim 1, it is characterized in that: described switch element is anchor clamps, is clamped in the clamping part of described proximal part.
4. device as claimed in claim 1 or 2 is characterized in that: the physico chemical factor control assembly of described a plurality of positions and switch element be integrated interlock so that a plurality of test tubes are carried out operation repetitive simultaneously.
5. device as claimed in claim 1 or 2, it is characterized in that: described multiposition operating platform is a porous plate, each pore volume is received a described test tube, and a plurality of described switch elements are comprised the flat board of porous by unitary construction for another, each borehole jack of described flat board is at the proximal part of each test tube, the folding of the porous on the described flat board is controlled by an elastic device, repeatedly makes the porous on this flat board open simultaneously or close by this elastic device being applied or removes external force.
6. device as claimed in claim 1 or 2, it is characterized in that: the outer wall of the distal portions of described test tube is pasted with the magnet steel that can be withdrawn.
7. device as claimed in claim 6, it is characterized in that: the distal portions of described test tube is made by hard material, and described distal portions comprises bottom and sidewall, thereby bottom and the distance of partial sidewall and angle become a long inclined-plane greater than distance and the angle of itself and other partial sidewall, and the described magnet steel that can be withdrawn is attached on the outer wall on described long inclined-plane.
8. device as claimed in claim 7 is characterized in that: described long inclined-plane forms the settling zone at a lower end and sidewall.
9. device as claimed in claim 5 is characterized in that: connect one below the described porous plate for the support of fixing and support described test tube.
10. device as claimed in claim 5, it is characterized in that: the distal portions of described test tube is made by hard material, and described distal portions comprises bottom and sidewall, thereby the distance of bottom and partial sidewall and angle become a long inclined-plane greater than distance and the angle of itself and other partial sidewall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013201243957U CN203244989U (en) | 2010-05-31 | 2011-05-31 | Device used for performing parallel operation of mixing and extracting multiple liquid samples |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010189028.6 | 2010-05-31 | ||
| CN2010101890286A CN101869855B (en) | 2010-05-31 | 2010-05-31 | Nucleic acid separation and detection method and test tube suitable for method |
| CN2013201243957U CN203244989U (en) | 2010-05-31 | 2011-05-31 | Device used for performing parallel operation of mixing and extracting multiple liquid samples |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201190000533XU Division CN203030295U (en) | 2010-05-31 | 2011-05-31 | Test tube for mixing and extracting liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203244989U true CN203244989U (en) | 2013-10-23 |
Family
ID=42995051
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101890286A Active CN101869855B (en) | 2010-05-31 | 2010-05-31 | Nucleic acid separation and detection method and test tube suitable for method |
| CN201190000533XU Expired - Lifetime CN203030295U (en) | 2010-05-31 | 2011-05-31 | Test tube for mixing and extracting liquid |
| CN2013201243957U Expired - Lifetime CN203244989U (en) | 2010-05-31 | 2011-05-31 | Device used for performing parallel operation of mixing and extracting multiple liquid samples |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101890286A Active CN101869855B (en) | 2010-05-31 | 2010-05-31 | Nucleic acid separation and detection method and test tube suitable for method |
| CN201190000533XU Expired - Lifetime CN203030295U (en) | 2010-05-31 | 2011-05-31 | Test tube for mixing and extracting liquid |
Country Status (2)
| Country | Link |
|---|---|
| CN (3) | CN101869855B (en) |
| WO (1) | WO2011150833A1 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232476A (en) * | 2014-09-17 | 2014-12-24 | 深圳市宝安区沙井人民医院 | High-channel nucleic acid extraction and purification device |
| CN105944789A (en) * | 2016-06-12 | 2016-09-21 | 青岛市中心血站 | Medical test tube rack |
| WO2019071450A1 (en) * | 2017-10-11 | 2019-04-18 | Shanghai Rendu Biotechnology Co., Ltd. | Automated nucleic acid sample preparation, detection, and analysis system |
| CN110869130A (en) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | Magnetically assisted separation device and related methods |
| CN116930088A (en) * | 2023-09-15 | 2023-10-24 | 佳木斯大学 | Device and method for detecting content of polysaccharide component in plant extract |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869855B (en) * | 2010-05-31 | 2013-04-03 | 嘉兴凯实生物科技有限公司 | Nucleic acid separation and detection method and test tube suitable for method |
| CN103013814B (en) * | 2012-12-20 | 2014-07-02 | 嘉兴凯实生物科技有限公司 | Nucleic acid purification device |
| CN104479993B (en) * | 2014-12-16 | 2016-04-06 | 嘉兴凯实生物科技有限公司 | A kind of instrument for extracting nucleic acid nucleic acid-extracting apparatus |
| CN104593252B (en) * | 2015-01-12 | 2017-02-22 | 国家纳米科学中心 | Sample pretreatment and amplification integrated nucleic acid analysis system based on pipette tip and application of system |
| CN104815583B (en) * | 2015-05-25 | 2017-02-08 | 刘瑞霞 | Test device for internal medicine |
| CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
| CN106281977B (en) * | 2016-11-22 | 2019-01-04 | 安图实验仪器(郑州)有限公司 | Double nucleic acid extraction box suitable for diagnostic nucleic acid system |
| CN106367310B (en) * | 2016-11-22 | 2018-05-29 | 安图实验仪器(郑州)有限公司 | For in-vitro diagnosis equipment and with square aperture reaction warehouse |
| CN106399054B (en) * | 2016-11-22 | 2019-01-04 | 安图实验仪器(郑州)有限公司 | For in-vitro diagnosis equipment and with the reaction warehouse of shaped open |
| CN106367309B (en) * | 2016-11-22 | 2019-01-04 | 安图实验仪器(郑州)有限公司 | Single nucleic acid extraction box suitable for diagnostic nucleic acid system |
| WO2018185908A1 (en) * | 2017-04-06 | 2018-10-11 | 株式会社島津製作所 | Magnetic particle operation device |
| CN109307653A (en) * | 2017-07-27 | 2019-02-05 | 国家纳米科学中心 | Capillary detection platform based on ring mediated isothermal nucleic acid amplification |
| CN107309001A (en) * | 2017-08-08 | 2017-11-03 | 安阳工学院 | A kind of townhouse formula centrifuge tube group |
| CN107560924A (en) * | 2017-10-13 | 2018-01-09 | 孔治 | A kind of suspended matter mechanical mixing apparatus and the mixer using the device |
| CN110042039A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of multi-functional sample processing apparatus |
| CN109395875B (en) * | 2018-12-11 | 2023-10-20 | 苏州英赛斯智能科技有限公司 | Magnetic bead separation mechanism, device and magnetic bead separation method |
| CN111363672B (en) * | 2020-04-15 | 2023-04-07 | 湖州市妇幼保健院 | Quick nucleic acid extraction equipment |
| CN113512487A (en) * | 2021-04-14 | 2021-10-19 | 新羿制造科技（北京）有限公司 | Nucleic acid extraction card box |
| CN114591824A (en) * | 2022-03-10 | 2022-06-07 | 广东和信健康科技有限公司 | Detection consumable and detection device |
| EP4327944A1 (en) * | 2022-08-23 | 2024-02-28 | Eppendorf SE | Microplate |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2037981U (en) * | 1988-12-05 | 1989-05-24 | 韩建礼 | Plastic negative pressure test tube |
| US5707860A (en) * | 1996-03-12 | 1998-01-13 | Becton Dickinson And Company | Vehicle for delivery of particles to a sample |
| FI108232B (en) * | 2000-02-16 | 2001-12-14 | Bio Nobile Oy | Procedure for closing and opening an opening |
| DE10231659B4 (en) * | 2002-07-12 | 2006-01-19 | Preanalytix Gmbh | A composition for binding nucleic acid to a solid phase |
| CN2608801Y (en) * | 2003-04-11 | 2004-03-31 | 孙银苟 | Trisheet type rubber pipe clamping head |
| CN2737716Y (en) * | 2004-10-27 | 2005-11-02 | 赵晋荣 | Sealed storing device |
| KR100766103B1 (en) * | 2006-10-27 | 2007-10-17 | 박경주 | Experiment device for charles' law |
| FR2912727B1 (en) * | 2007-02-19 | 2013-07-19 | Cryo Bio System | PACKAGING TUBE OF A PREDETERMINED VOLUME OF BIOLOGICAL SUBSTANCE TO BE PRESERVED AT LOW TEMPERATURE AND SYSTEM COMPRISING SAME |
| CN201182601Y (en) * | 2008-03-18 | 2009-01-21 | 刘淑美 | Negative-pressure hemospasia tube |
| CN201201943Y (en) * | 2008-04-08 | 2009-03-04 | 上海理工大学 | Nucleic acid extracting system apparatus |
| CN101502810A (en) * | 2009-02-03 | 2009-08-12 | 范志鹏 | Experimental laboratory limestone dry distillation instrument as well as manufacturing process and use |
| CN101869855B (en) * | 2010-05-31 | 2013-04-03 | 嘉兴凯实生物科技有限公司 | Nucleic acid separation and detection method and test tube suitable for method |
-
2010
- 2010-05-31 CN CN2010101890286A patent/CN101869855B/en active Active
-
2011
- 2011-05-31 CN CN201190000533XU patent/CN203030295U/en not_active Expired - Lifetime
- 2011-05-31 CN CN2013201243957U patent/CN203244989U/en not_active Expired - Lifetime
- 2011-05-31 WO PCT/CN2011/075017 patent/WO2011150833A1/en active Application Filing
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232476A (en) * | 2014-09-17 | 2014-12-24 | 深圳市宝安区沙井人民医院 | High-channel nucleic acid extraction and purification device |
| CN105944789A (en) * | 2016-06-12 | 2016-09-21 | 青岛市中心血站 | Medical test tube rack |
| CN110869130A (en) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | Magnetically assisted separation device and related methods |
| US11433402B2 (en) | 2017-07-19 | 2022-09-06 | Amgen Inc. | Magnetic assisted separation apparatuses and related methods |
| WO2019071450A1 (en) * | 2017-10-11 | 2019-04-18 | Shanghai Rendu Biotechnology Co., Ltd. | Automated nucleic acid sample preparation, detection, and analysis system |
| CN116930088A (en) * | 2023-09-15 | 2023-10-24 | 佳木斯大学 | Device and method for detecting content of polysaccharide component in plant extract |
| CN116930088B (en) * | 2023-09-15 | 2024-01-16 | 佳木斯大学 | Device and method for detecting content of polysaccharide component in plant extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101869855B (en) | 2013-04-03 |
| CN101869855A (en) | 2010-10-27 |
| WO2011150833A1 (en) | 2011-12-08 |
| CN203030295U (en) | 2013-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203244989U (en) | Device used for performing parallel operation of mixing and extracting multiple liquid samples | |
| CN105562132B (en) | A kind of device extracted and detect biological sample | |
| CN111876468B (en) | Full-automatic nucleic acid detection method and test tube | |
| CN108660074B (en) | Integrated solution for nucleic acid extraction PCR amplification detection | |
| EP2215103B1 (en) | Method for purifying nucleic acids | |
| US9250169B2 (en) | Selective capture and release of analytes | |
| EP1740720A2 (en) | Method and device for sample preparation control | |
| CN108517290A (en) | A kind of five chamber nucleic acid-extracting apparatus and method based on immiscible phase interfacial tension | |
| CN110167674B (en) | System and method for isolating substances in bulk liquids | |
| CN116445258A (en) | PCR tube and detection method thereof applied to target nucleic acid molecules | |
| CN105126793B (en) | A kind of preparation method based on single stranded DNA nucleic acid aptamers modification hybrid quartz capillary integral post | |
| CN113528625A (en) | Microfluidic nucleic acid detection method and application | |
| US20170067807A1 (en) | Separation and assay of target entities using filtration membranes comprising a perforated two-dimensional material | |
| CN114276915A (en) | Chip and kit for detecting novel coronavirus nucleic acid, preparation method and application | |
| CN113552254A (en) | Method for detecting polycyclic musk in water body | |
| JP2021047170A (en) | Microfluidic device for preparing and analyzing biological sample | |
| AU2019269296A1 (en) | Systems and methods for nucleic acid purification using flow cells with actuated surface-attached structures | |
| RU2451747C2 (en) | Method for homogenising biological samples containing magnetic nanoparticles when extracting dna during automatic sample preparation for pcr analysis | |
| CN103083940A (en) | Microwave-assisted-hollow filter-liquid/liquid micro-extraction device and application | |
| CN205774476U (en) | Dissociative DNA extraction system | |
| Kim et al. | A microchip for nucleic acid isolation and enrichment | |
| Chen et al. | Microdevice-based DNA extraction method using green reagent | |
| AU2015252784A1 (en) | Separation and assay of target entities using filtration membranes comprising a perforated two-dimensional material | |
| Sugishita et al. | A centrifugation-based method for high-throughput biomaterial separation using magnetic microbeads | |
| EP4330659A1 (en) | Sample transfer method and system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: JINGJIANG HUAYU YANGGUANG MEDICAL EQUIPMENT CO., L Free format text: FORMER OWNER: SHANGHAI RENDU BIOTECHNOLOGY CO., LTD. Effective date: 20150203 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 201203 PUDONG NEW AREA, SHANGHAI TO: 214500 TAIZHOU, JIANGSU PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150203 Address after: Jingjiang City, Jiangsu province 214500 students in Taizhou City Hall Zhenjiang Road No. 78 Patentee after: Hua Yu sunlight Medical Devices Co., Ltd. of Jingjiang City Address before: 201203 Shanghai City, Pudong New Area Zhangjiang hi tech Park East Ruiqinglu No. 590 building 7 layer 3 Patentee before: Shanghai Rendu Biotechnology Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131023 |