CN203235572U - Centrifugal bone marrow cell collecting device - Google Patents
Centrifugal bone marrow cell collecting device Download PDFInfo
- Publication number
- CN203235572U CN203235572U CN 201320262759 CN201320262759U CN203235572U CN 203235572 U CN203235572 U CN 203235572U CN 201320262759 CN201320262759 CN 201320262759 CN 201320262759 U CN201320262759 U CN 201320262759U CN 203235572 U CN203235572 U CN 203235572U
- Authority
- CN
- China
- Prior art keywords
- bone marrow
- centrifugal
- marrow cell
- collecting pipe
- sedimentator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Centrifugal Separators (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a centrifugal bone marrow cell collecting device. The centrifugal bone marrow cell collecting device is composed of a centrifugal collecting pipe and a centrifuging device, wherein the centrifuging device is sleeved with the centrifugal collecting pipe, can be drawn out in the direction of the longitudinal axis of the centrifugal collecting pipe, and is composed of an isolator, clamping grooves symmetrically arranged along the longitudinal axis of the isolator and a hopper. The centrifugal bone marrow cell collecting device achieves the purpose of separating and collecting bone marrow cells according to the principle that the bone marrow cells are centrifuged to exterior of long bones through centrifugal force and low-speed centrifuging will not damage cell viability. The centrifugal bone marrow cell collecting device is strong in operability, reduces damage of violent manual factors to the bone marrow cells and pollution indexes of the bone marrow cells, can also separate and extract bone marrow cells of small animals in a large-batch mode, can be repeatedly used after being sterilized under a high temperature, has the advantages of being high in experiment efficiency, reducing damage to the cells and pollution and the like, and is used for separating and extracting experiments of bone marrow cells of long bones of small animals like mice, rats and guinea pigs.
Description
Technical field
The utility model relates to a kind of bone marrow cell centrifugal collector, tests in particular for toy (such as mouse, rat, cavy etc.) long bone bone marrow cell separation and Extraction.
Background technology
The study general that the toy stem cell is collected experimental technique is separation and Extraction from its long bone marrow.Traditional means of experiment mainly is by isolate the toy long bone under gnotobasis, first long bone is cut off from the centre, then after its epiphysis end is cut off an osculum, insert the long bone broken ends of fractured bone with the syringe needle that fills cell culture medium, by the method for syringe high pressure washing its bone marrow cell is gone out.
The aforementioned conventional experimental technique is owing to be subject to the restriction of sterile working, syringe needle size and flushing marrow efficient etc., and conventional efficient is low, and pollutes easily.
The utility model content
The deficiencies such as, easy pollution low in separation and Extraction marrow efficient for prior art, according to embodiment of the present utility model, be desirable to provide a kind of effect of both having brought into play the separated and collected bone marrow cell, reduced again the not enough new type bone myelocyte centrifugal collector such as direct flushing bone marrow cell and pollution.
According to embodiment, a kind of bone marrow cell centrifugal collector that the utility model provides is made of centrifugal collecting pipe and sedimentator, and sedimentator is set in the centrifugal collecting pipe, and sedimentator can be extracted out along centrifugal collecting pipe y direction; Sedimentator is reached along draw-in groove and the funnel of isolator longitudinal axis symmetric arrays by isolator and consists of.
With respect to prior art, thereby the utility model utilizes centrifugal force to reach the purpose that principle that low-speed centrifugal can not destroy cytoactive reaches the separated and collected bone marrow cell with the marrow inner cell is centrifugal to long bone.The utility model is workable, has reduced because the people is the damage of violence factor to bone marrow cell, reduces the pollution index of bone marrow cell, can carry out separation and Extraction to toy bone marrow cell in enormous quantities simultaneously.In addition, but reuse, have conventional efficient high behind the utility model centrifugal collector autoclave sterilization, reduce advantages such as the damage of cell and pollutions.
Description of drawings
Fig. 1 is the front section view of the utility model bone marrow cell centrifugal collector.
Fig. 2 is the front section view of sedimentator 2 in the utility model bone marrow cell separated and collected.
Among the figure: 1 is centrifugal collecting pipe; 2 is sedimentator; 3 is the toy long bone; 4 is bone marrow cell; 5 is isolator; 6 is draw-in groove; 7 is the lid of centrifugal collecting pipe; 8 is funnel.
The specific embodiment
Below in conjunction with the drawings and specific embodiments, further set forth the utility model.These embodiment are interpreted as only being used for explanation the utility model and are not used in restriction protection domain of the present utility model.After the content of having read the utility model record, those skilled in the art can make various changes or modifications the utility model, and these equivalences change and modification falls into the utility model claim limited range equally.
Shown in Fig. 1-2, the bone marrow cell centrifugal collector that the utility model preferred embodiment provides by can the centrifugal collecting pipe 1 of 1.5mL high temperature resistant, autoclave sterilization and sedimentator 2 consist of, sedimentator 2 is set in the centrifugal collecting pipe 1, sedimentator 2 is reached along draw-in groove 6 and the funnel 8 of isolator longitudinal axis symmetric arrays by isolator 5 and consists of, and sedimentator 2 can be extracted out along centrifuge tube 1 y direction.Sedimentator 2 can be extracted out along centrifugal collecting pipe 1 y direction, is convenient to the collection of follow-up bone marrow cell.
When separating bone marrow cell osculum is opened in toy long bone two ends under aseptic condition, then put it in the sedimentator 2 with lid, the lid 7 of the centrifugal collecting pipe that closes is to prevent pollution.Funnel 8 prevents that the toy long bone from revealing into centrifugal collecting pipe 1 bottom.
After above step is carried out, can put into the 1.5mL centrifuge with 3000rpm low-speed centrifugal 1-2 minute, treat centrifugal finishing, just can under aseptic condition, sedimentator 2 and inner long bone be taken out the bone marrow cell 4 of centrifugal collecting pipe 1 bottom in the lump.
Specify the process that the utility model is used for centrifugal collection bone marrow cell below in conjunction with accompanying drawing.
Shown in Fig. 1-2, during experiment, experiment operator only needs osculum is opened in toy long bone two ends under aseptic condition, then puts it in the sedimentator 2 with lid, and the lid 7 of the centrifugal collecting pipe that closes is to prevent pollution.Then put into the 1.5mL centrifuge with 3000rpm low-speed centrifugal 1-2 minute, treat centrifugal finishing, just can under aseptic condition sedimentator 2 and inner long bone be taken out in the lump, 4 are the bone marrow cell 4 of centrifugal collecting pipe 1 bottom.With namely reusable behind centrifugal collecting pipe 1 and sedimentator 2 autoclave sterilizations.
Claims (4)
1. a bone marrow cell centrifugal collector is characterized in that, is made of centrifugal collecting pipe and sedimentator, and sedimentator is set in the centrifugal collecting pipe, and sedimentator can be extracted out along centrifugal collecting pipe y direction; Sedimentator is reached along draw-in groove and the funnel of isolator longitudinal axis symmetric arrays by isolator and consists of.
2. bone marrow cell centrifugal collector according to claim 1 is characterized in that, centrifugal collecting pipe and sedimentator are made by high temperature resistant, high voltage bearing material.
3. bone marrow cell centrifugal collector according to claim 1 is characterized in that, the volume of centrifugal collecting pipe is 1.5mL.
4. bone marrow cell centrifugal collector according to claim 1 is characterized in that sedimentator has lid, and centrifugal collecting pipe has lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320262759 CN203235572U (en) | 2013-05-15 | 2013-05-15 | Centrifugal bone marrow cell collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320262759 CN203235572U (en) | 2013-05-15 | 2013-05-15 | Centrifugal bone marrow cell collecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203235572U true CN203235572U (en) | 2013-10-16 |
Family
ID=49312908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320262759 Expired - Lifetime CN203235572U (en) | 2013-05-15 | 2013-05-15 | Centrifugal bone marrow cell collecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203235572U (en) |
-
2013
- 2013-05-15 CN CN 201320262759 patent/CN203235572U/en not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2009019686A3 (en) | Sweat collectors and methods of collecting sweat | |
| CN103997974A (en) | Systems and methods for processing cells | |
| CN204602344U (en) | The electronic crushing/dewatering device of a kind of changing food waste | |
| CN104630204A (en) | Extraction method of oil reservoir microbial genome DNA | |
| CN206560700U (en) | A kind of effective ingredients in plant extractor | |
| EP2503007A1 (en) | Method for separating stem cells | |
| CN206289239U (en) | DNA of plants rapid extraction corollary apparatus | |
| CN203396606U (en) | Watermelon sampler | |
| CN203235572U (en) | Centrifugal bone marrow cell collecting device | |
| CN102838758B (en) | Method of preparing gutta-percha with eucommia leaves | |
| CN204150517U (en) | Medical sharp apparatus separated and collected case | |
| CN201453726U (en) | Autogenous bone marrow blood refluxing device | |
| CN204165815U (en) | Streaming cell filtration head | |
| CN207908243U (en) | A kind of separation and Extraction kit of excretion body | |
| CN104359738B (en) | A kind of processing method suitable for flow cytometry plant drying tissue | |
| CN106076473A (en) | A kind of glass breaker | |
| KR101390920B1 (en) | The device for fat disrupting and the element separator which has the function of fat disrupting | |
| CN206143221U (en) | Eccentric large capacity blake bottle and centrifuge adapter assembly thereof | |
| CN204746353U (en) | Centrifuging tube | |
| CN205234649U (en) | Medical multifunctional wastebasket | |
| CN107828726A (en) | A kind of method of the fractionation of fatty mescenchymal stem cell from fat | |
| CN206304413U (en) | A kind of filter membrane pressurizing filter device | |
| CN208287008U (en) | A kind of blood collection traditional vacuum pipe | |
| CN103316889A (en) | Environmentally friendly treatment method for kitchen waste | |
| CN203408068U (en) | Disposable sterile cervix sampler |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131016 |