CN203128566U - Anaerobic strain preservation bottle - Google Patents
Anaerobic strain preservation bottle Download PDFInfo
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- CN203128566U CN203128566U CN 201320104043 CN201320104043U CN203128566U CN 203128566 U CN203128566 U CN 203128566U CN 201320104043 CN201320104043 CN 201320104043 CN 201320104043 U CN201320104043 U CN 201320104043U CN 203128566 U CN203128566 U CN 203128566U
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- bottle
- bottleneck
- anaerobic
- inner plug
- plug
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Abstract
The utility model relates to an anaerobic strain preservation bottle which comprises an inner plug connecting a cover body and a cylinder body, a cylindrical bottle body with a bottleneck and a round cover, wherein the bottle body is connected with the bottle opening through the bottleneck; the inner plug is arranged in the bottle opening; and the round cover is arranged on the inner plug. The anaerobic strain preservation bottle is characterized in that the inner plug is provided with a matched aluminum envelope; the aluminum envelope is connected with the center of the inner wall of the round cover; the inner diameter of the aluminum envelope is equal to the cover diameter of the cover body in the inner plug and the outer diameter of the bottle opening; the outer diameter of the cylinder body in the inner plug is equal to the inner diameter of the bottle opening; and the length of the bottleneck is less than the length of the inner plug. The anaerobic strain preservation bottle provided by the utility model has the advantages of easiness in cleaning, simplicity and convenience in operation, rare pollution, safety in use, saving of storage space, complete conformation to anaerobic conditions and the like, can be widely used for culturing and observing aerobic, anaerobic or facultative anaerobic microorganisms, and is suitable for long-time preservation of large-scale strains under anaerobic conditions.
Description
Technical field
The utility model relates to the long-term preservation device of very low temperature anaerobism of a kind of microbial strains, relates in particular to a kind of anaerobic species preservation bottle.
Background technology
All are from the bacterial classification with good character of production practice or Science Institute's acquisition, comprising the wild-type strain that obtains from the direct separation of nature, and the good dissociant that seed selection is come out through manual method or engineering strain etc. all are the important germ plasm resource of country.Therefore, culture presevation is the basis of all microorganisms work, the purpose of culture presevation be not dead after making bacterial classification by preservation, do not make a variation, not by living contaminants, and keep its good character, be beneficial to produce the application with scientific research.
The principle of culture presevation is in order to reach long-term maintenance bacterial classification good characteristic, key problem is to reduce the bacterial classification aberration rate, and the variation of bacterial classification mainly betides the vigorous growth and breeding process of microorganism, it is general when microbial metabolism is vigorous, the speed of mutation of bacterial classification is just fast, otherwise Metabolic activity is faint, and speed of mutation is just slow.Therefore in preserving process, must create a kind of environment, make microorganism be in metabolic minimum level, growth and breeding disabled state, it is not morphed and keep active.Culture collection process is a lot of at present, but mainly designs according to following principle: ⑴ must select for use typical good pure growth to carry out preservation, and adopts its hypopus as far as possible; ⑵ create the envrionment conditions that is conducive to microorganism dormancy, as low temperature, drying, anoxic, shortage nutrition and interpolation protective material etc.; ⑶ reduce passage number as far as possible.In addition, in the anaerobion cell of strictness, there are not superoxide dismutase activity and catalase activity, and these two kinds of enzymes are directly relevant with the metabolism of the derivative of intracellular oxygen and oxygen, therefore the oxygen of denier just can make the vital movement of anaerobion be suppressed in the environment, even causes its death.
At present, common culture presevation form is that test tube, culture dish, sandy soil pipe, freeze-drying pipe, peace are cutd open bottle, cillin bottle etc., wherein test tube, culture dish floor space big, easily broken, be unfavorable for microorganism dormancy, easily pollute, can not the anoxic preservation; The husky technology of sandy soil Guan Xuansha system is loaded down with trivial details, can not prolonged preservation; The not easy cleaning packing of freeze-drying pipe, freeze-drying efficient are low; It is easily broken that peace is cutd open pipe, and switching is easily polluted, and a pipe can only use once, and storage takes up room big, and complex steps when opening; The patent No. is the described cillin bottle of CN201400683, needs specific equipment and supporting freeze-drying sleeve pipe during freeze-drying, and equipment manufacturing cost is expensive and purchase difficulty, and introduces living contaminants easily in operating process.
The utility model content
Technical problem to be solved in the utility model provides and a kind ofly is easy to clean, easy to operate, the anaerobic species preservation bottle that is difficult for polluting, can creating anaerobic condition.
For addressing the above problem, a kind of anaerobic species preservation bottle described in the utility model comprises the interior plug that linked together by lid and cylinder, cylindrical and be provided with bottle and the dome of bottleneck; Described bottle is connected with bottleneck by described bottleneck; Be provided with described interior plug in the described bottleneck, be provided with described dome in this beyond the Great Wall, it is characterized in that: be provided with an aluminum big envelope that matches in described beyond the Great Wall, this aluminum big envelope links to each other with the inwall circle centre position of described dome; The internal diameter of described aluminum big envelope with described in the plug lid footpath of lid and the external diameter of described bottleneck all equate; The external diameter of cylinder equates with the internal diameter of described bottleneck in filling in described; The length of described bottleneck is less than the length of plug in described.
The volume of described bottle is 2.0 ~ 4.0ml.
The edge of described aluminum big envelope from the tie point of itself and described dome to described aluminum big envelope do not have indentation.
The utility model compared with prior art has the following advantages:
1, since in the utility model in internal diameter and the interior plug of aluminum big envelope the lid of lid directly and the external diameter of bottleneck all equate, the external diameter of cylinder equates with the internal diameter of bottleneck in the interior plug, the length of bottleneck is less than the length of plug in described, therefore, can make bottle mouth position each several part closure fully, reach the purpose of complete anaerobism.
2, since in the utility model the edge of aluminum big envelope from the tie point of itself and dome to the aluminum big envelope do not have indentation, therefore, when dome was started, the aluminum big envelope is unlikely to be opened simultaneously, the sealing that is conducive to whole bottle is complete, fully reaches the condition of anaerobism.
3, the utility model is according to the principle of very low temperature culture presevation commonly used, avoided traditional bacterial classification save set be difficult for to open, not easy cleaning, pollute shortcomings such as bacterial classification, very low temperature preservation for a long time easily, have be easy to clean, easy and simple to handle, be difficult for polluting, use safety, save storage space, can reach advantage such as anaerobic condition fully, can be widely used in cultivation and the observation of aerobic, anaerobism or amphimicrobe, be fit to the long-term preservation of bacterial classification under fairly large, the anaerobic condition.
Description of drawings
Below in conjunction with accompanying drawing embodiment of the present utility model is described in further detail.
Fig. 1 is structural representation of the present utility model.
Among the figure: 1-aluminum big envelope 2-interior plug 3-bottleneck 4-bottleneck 5-bottle 6-dome.
Embodiment
As shown in Figure 1, a kind of anaerobic species preservation bottle comprises the interior plug 2 that linked together by lid and cylinder, cylindrical and be provided with bottle 5 and the dome 6 of bottleneck 4.Bottle 5 is connected with bottleneck 3 by bottleneck 4; Plug 2 in being provided with in the bottleneck 3, plug 2 is provided with dome 6 in this; Interior plug 2 is provided with an aluminum big envelope 1 that matches, and this aluminum big envelope 1 links to each other with the inwall circle centre position of dome 6; The external diameter of the lid of lid footpath and bottleneck 3 all equates in the internal diameter of aluminum big envelope 1 and the interior plug 2; The external diameter of cylinder equates with the internal diameter of bottleneck 3 in the interior plug 2; The length of bottleneck 4 is less than the length of interior plug 2.
Wherein: the volume of bottle 5 is 2.0 ~ 4.0ml.
The edge of aluminum big envelope 1 from the tie point of itself and dome 6 to aluminum big envelope 1 do not have indentation.
Claims (3)
1. anaerobic species preservation bottle comprises the interior plug (2) that linked together by lid and cylinder, cylindrical and be provided with bottle (5) and the dome (6) of bottleneck (4); Described bottle (5) is connected with bottleneck (3) by described bottleneck (4); Be provided with described interior plug (2) in the described bottleneck (3), should be provided with described dome (6) by interior plug (2), it is characterized in that: plug (2) is provided with an aluminum big envelope (1) that matches in described, and this aluminum big envelope (1) links to each other with the inwall circle centre position of described dome (6); The internal diameter of described aluminum big envelope (1) all equates with the lid footpath of the middle lid of described interior plug (2) and the external diameter of described bottleneck (3); The external diameter of the middle cylinder of plug (2) equates with the internal diameter of described bottleneck (3) in described; The length of described bottleneck (4) is less than the length of plug (2) in described.
2. a kind of anaerobic species preservation bottle as claimed in claim 1, it is characterized in that: the volume of described bottle (5) is 2.0 ~ 4.0ml.
3. a kind of anaerobic species preservation bottle as claimed in claim 1 is characterized in that: the edge of described aluminum big envelope (1) from the tie point of itself and described dome (6) to described aluminum big envelope (1) do not have indentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320104043 CN203128566U (en) | 2013-03-07 | 2013-03-07 | Anaerobic strain preservation bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320104043 CN203128566U (en) | 2013-03-07 | 2013-03-07 | Anaerobic strain preservation bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203128566U true CN203128566U (en) | 2013-08-14 |
Family
ID=48936562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320104043 Expired - Fee Related CN203128566U (en) | 2013-03-07 | 2013-03-07 | Anaerobic strain preservation bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203128566U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651230B (en) * | 2015-03-09 | 2016-06-15 | 香红星 | Spontaneous hot liquid cultivates the apparatus and method of active anaerobion |
-
2013
- 2013-03-07 CN CN 201320104043 patent/CN203128566U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651230B (en) * | 2015-03-09 | 2016-06-15 | 香红星 | Spontaneous hot liquid cultivates the apparatus and method of active anaerobion |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130814 Termination date: 20170307 |
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| CF01 | Termination of patent right due to non-payment of annual fee |