CN203116704U - Dynamic lens contour capture device in in-situ liquid forming optical microlens manufacturing - Google Patents
Dynamic lens contour capture device in in-situ liquid forming optical microlens manufacturing Download PDFInfo
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- CN203116704U CN203116704U CN 201320070292 CN201320070292U CN203116704U CN 203116704 U CN203116704 U CN 203116704U CN 201320070292 CN201320070292 CN 201320070292 CN 201320070292 U CN201320070292 U CN 201320070292U CN 203116704 U CN203116704 U CN 203116704U
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Abstract
The utility model belongs to the field of biology, analytical chemistry and medicine detection, and discloses a dynamic lens contour capture device in in-situ liquid forming optical microlens manufacturing. The dynamic lens contour capture device is used for monitoring manufacturing of an in-situ forming optical microlens in real time so as to guarantee real-time control of aspheric working surface curvature and dimensional accuracy of the in-situ forming optical microlens, and focusing efficiency of excitation fluorescence of a detected object and micro-spectrum detection sensitivity of a biochip are improved. The device comprises a light source and a camera, three daylighting modes, including a backlight method, a direct side light method and a reflecting side light method, are designed according to a daylighting theory of shooting a transparent object, and a light softening board, a light barrier, a light barrier and a reflector and the light source are adopted respectively. The devices are suspended on a rotating support, and a stepping motor drives rotational shooting around detected transparent liquid drops so as to obtain a 360-degree azimuthal image. A polaroid is arranged in front of the camera, black visors are arranged on the periphery of a lens of the cameral, and stray light generated by the transparent liquid drops is avoided effectively.
Description
Technical field
The present invention proposes lens dynamic outline trap setting in a kind of in-situ liquid shaping optical microlens manufacturing, be mainly used in the manufacturing of original position shaping optical microlens is monitored in real time, curvature and dimensional accuracy with the aspheric curve that guarantees original position shaping optical microlens reach technical requirement, belong to biology and analytical chemistry and medical science detection range.
Background technology
The microorganism chip is a new and high technology that has developed rapidly in recent years in life science, its essence is: on microminiaturized basis, whole biochemical analysis processes and whole laboratory function (as: are sampled, dilution, add reagent, reaction, separate and detection etc.) integrated (embedding) in the chip of stamp or credit card-sized, thereby generically be called " chip lab ".Its science and advance embody a concentrated reflection of miniature and integrated these two aspects of function of structure.Input wherein is the important component part of biochip technology, mainly comprises signal generation, signal collection and transmission, signal processing and identifies three parts.In various biochip signal detecting methods, fluorescence low-light spectrum detection method have selectivity good, can make micro-qualitative and quantitative analysis, have advantage such as non-destructive, become one of most widely used general, detection technique that sensitivity is the highest in the biochip field.
Photomultiplier (PMT) or the charge coupled cells (CCD) of using carry out opto-electronic conversion more in the little detection system of spectrum at present, these elements and supporting light path system volume thereof are big, can not embed in the biochip, hinder the integrated raising of biochip widely, become the bottleneck of biochip development.Therefore, little to reach the little detection system of spectrum of biotechnology requirement imperative to embedding chip and sensitivity high energy for the development volume.Present correlative study both domestic and external still is in the elementary exploratory stage.When the fluorescence spectrum of biochip detected, the faint reason of fluorescence signal was that the measured object amount is few, luminous little, and was not the fluorescence signal intensity reduction of unit volume.Use the optical microlens of particular curvature shape can increase the photon collection total amount of system, and make lenticule and testing end face accurately with the bonding light intensity focusing efficiency that increases system of optical axis.Therefore, obtaining particular optical lenticule and realization high precision bonding with optical axis is the volume characteristic size improves the long-pending little detection sensitivity of spectrum of microbody when millimeter and submillimeter magnitude effective means.
Optical microlens has multiple production Technology at present, and main method has: optical resin droplet spray printing method, thermoplastic film method for making, the multilayer photoengraving method of forming.The total characteristics of above-mentioned several processes are that elder generation at other base material makes optical microlens separately, and then it is moved on the optical filter of optical excitation unit or optical detecting unit, uses the optics glue bond.These methods of making optical microlenses all face two common technical barriers: the one, when the optical microlens of making being peeled off the making base material, be difficult to guarantee lens bottom water plane flatness and and optical axis between verticality.The 2nd, when the optical filter of lenticule and optical excitation unit or optical detecting unit was bonding, the excitation source in the optical axis that is difficult to guarantee optical microlens and the optical excitation unit or the central symmetry axis of the electrooptical device in the optical detecting unit were accurately aimed at.Original position forming process optical microlens of the present invention can overcome above-mentioned two hang-ups, realizes that high precision is bonding with optical axis.Specific embodiment is, the ultra-violet curing optical cement that mixes the quartzy nano particle of certain proportion is dropped on the former allocation of chip from certain altitude release, when glue drop in workplace from top to bottom and to around when trickling diffusion, in order to keep potential energy minimum, its surperficial curve shape changes with surface tension of liquid, but remain center symmetry and surface area minimum, Ultra-Violet Laser irradiation in time is solidified into the optical microlens of the design shape that coincide with it.This method helps the little detection system of gene by fluorescence to realize the long-pending and high sensitivity of microbody, and namely volume is small in being directly embedded in the biochip, and is highly sensitive to the technical requirement that can satisfy the microorganism input.
Though can make optical microlens and little testing end face bonding with optical axis accurately though the original position forming process is made lenticule.But be faced with another technical barrier in making the lenticule process: lenticular non-ball working curved surface curvature and dimensional accuracy influence the focusing effect of lens, and this is one of key that influences low-light spectrum detection sensitivity in the biochip.When processing and fabricating original position forming microlens, because some factor (as non-homogeneous curing or inner and surperficial shrink tension difference and when controlling contact angle substrate interface property difference etc.), cause the lenticular non-ball working curved surface curvature of original position and the dimensional accuracy of laser curing can not reach technical requirement on design, make the original position forming microlens reduce the focusing efficiency to light, detection sensitivity equally also will reduce, and this will make the sensitivity of low-light spectrum detection system can't satisfy the technical requirement that faint bio signal is detected.
Because lenslet dimension in the millimeter magnitude, has transparent character simultaneously, and the easy reflective characteristics of smooth surface, the seizure of its contour images is a difficult problem.Therefore, a kind of high speed, high-resolution transparent miniature working fluid dynamic outline capturing technology are very important to the manufacturing of original position shaping optical microlens.
Summary of the invention
Purpose of the present invention namely be to provide a kind of can 360 ° of monitoring devices that catch miniature flowable transparent liquid, the dynamic shape to forming lenticular optical cement when can be used for original position shaping optical microlens and making is monitored.
To achieve these goals, the utility model different daylighting method when transparent substance taken, taked lens dynamic outline trap setting in the manufacturing of three kinds of technical schemes design in-situ liquid shaping optical microlenses:
1, according to the back lighting method: allow light see through the transmittance softbox, as the unique light source that illuminates the ultra-violet curing optical cement.Lens dynamic outline capture system comprises light source, softbox, camera in the manufacturing of in-situ liquid shaping optical microlens, described light source, softbox, camera being aligned successively are arranged on the runing rest, and polaroid is set before the camera, the black shadow shield is set around the cam lens; Described support is by the stepper motor driven rotary, and 9 ° of cameras of the every rotation of support are taken once.Under this light condition, optical cement is reflective can be too not strong.The dark dark profile lines of optical cement can show preferably.
2, according to direct side light method: with the shooting background of black background paper as the ultra-violet curing optical cement, lens dynamic outline capture system comprises light source, light barrier, camera in the manufacturing of in-situ liquid shaping optical microlens, described light source, light barrier, camera being aligned successively are arranged on the runing rest, and the width of described light barrier is consistent with the shooting width of workpiece being shot, before the described camera polaroid is set, the black shadow shield is set around the cam lens; Described support is by the stepper motor driven rotary, and 9 ° of cameras of the every rotation of support are taken once.Blocked by the black background paper back side in the above-mentioned way that light directive ultra-violet curing optical cement is set, part light is injected lenticule by the background paper side and is held in the district, and soft light illumination is provided.
3, according to reflection side illumination explicit law: with the shooting background of black background paper as the ultra-violet curing optical cement, lens dynamic outline capture system comprises reflector, light source, light barrier, camera in the manufacturing of in-situ liquid shaping optical microlens, described reflector, light source, light barrier, camera being aligned successively are arranged on the runing rest, and described light source is invested described reflector, the width of described light barrier is consistent with the shooting width of workpiece being shot, before the described camera polaroid is set, the black shadow shield is set around the cam lens; Described support is by the stepper motor driven rotary, and 9 ° of cameras of the every rotation of support are taken once.Above-mentioned not direct directive ultra-violet curing optical cement one side of light that arranges, but the directive opposite side, after soft reflector reflection, part light is blocked by black background paper, part light is injected lenticule by the background paper side and is held in the district, and soft light illumination is provided.
Above-mentioned stepper motor can be with step angle θ=0.9 ° rotation, and rotating speed is 1000pps～3000pps.But this stepper motor driven rotary support is around 360 ° of rotations of subject.
The image analytic degree of above-mentioned CCD camera module is 8,000,000 pixels, and namely Horizontal number of pixels * Vertical number of pixels=3264*2448 is applicable to the shooting of micro lens and amplifies processing.Camera has the Automatic white balance adjustment, and the ratio that can regulate three primary colours under different colour temperature environment automatically reaches the balance of color.Ultra-violet curing optical cement being shot projects on the image sensor surface by the optical imagery that camera lens generates, transfer electric signal then to, through becoming data image signal after the A/D conversion, deliver to again and carry out the view data processing in the digital signal processing chip, offer quality control system and carry out quality control.
Polaroid in the above scheme just can be eliminated the reflective by force of lenticule surface, thereby eliminates or alleviate hot spot; Used in sunshade board is in the camera image of eliminating the optical cement reflection.
Beneficial effect of the present invention:
1, filled up in the original position shaping optical microlens manufacturing technology blank to the microlens shape precise monitoring;
2, can provide high-resolution lenticule image to carry out quality control treatments to the later stage quality control system, make quality control system form a complete closed-loop control system;
3, the resolution of the transparent miniature working fluid dynamic outline capture device of the present invention's employing reaches the micron number magnitude, satisfies the application requirements in the low-light spectrum detection system embedding biochip.
Description of drawings
Fig. 1 is the structural representation of the transparent miniature working fluid dynamic outline capture system of employing back lighting method or direct side light method;
Fig. 2 is for adopting the transparent miniature working fluid dynamic outline capture system principle schematic of back lighting method;
Fig. 3 is for adopting the transparent miniature working fluid dynamic outline capture system principle schematic of direct side light method;
Fig. 4 is the structural representation of the transparent miniature working fluid dynamic outline capture system of employing reflection side illumination explicit law;
Fig. 5 is the principle schematic of the transparent miniature working fluid dynamic outline capture system of employing reflection side illumination explicit law.
Among the figure: 1, light source, 2, light tool frame, 3, little testing end face, 4, ultra-violet curing optical cement (lenticule), 5, camera, 6, polaroid, 7, the black shadow shield, 8, softbox, 9, light barrier, 10, reflector, 11, stepper motor.
Embodiment
Be described in further details below in conjunction with the present invention of accompanying drawing 1-5.
The present invention is used for the manufacturing of original position shaping optical microlens, and wherein lenticule is formed by the optical cement curing of liquid state.Be specially the liquid ultra-violet curing optical cement 4 that will mix the quartzy nano particle of certain proportion and discharge from certain altitude and drop in little testing end face 3, then drop is carried out mouldingly, be cured after meeting the requirement of lenticule curved surface.Transparent miniature working fluid profile capture system of the present invention namely is used for monitoring to the moulding process of drop.
Transparent miniature working fluid profile capture system of the present invention adopts the rotating smooth tool frame of a lifting, each device is arranged on below the light tool frame in the system, and light tool frame is in ultra-violet curing optical cement 4 rotations that center under the drive of stepper motor 11 on little testing end face 3.According to different daylighting methods, native system can adopt three kinds of modes:
1, adopts the back lighting method, allow light see through the transmittance softbox as Fig. 1,2, as the unique light source that illuminates the ultra-violet curing optical cement.Each device comprises light source 1, softbox 8, camera 5, and described light source, softbox, camera are successively set on the light tool frame 2, and polaroid 6 is set before the camera 5, and black shadow shield 7 is set around the cam lens.
2, adopt direct side light method, as Fig. 1,3 with the shooting background of black background paper as the ultra-violet curing optical cement.Blocked by the black background paper back side in the way of light directive ultra-violet curing optical cement, part light is injected lenticule by the background paper side and is held in the district.Each device comprises light source 1, light barrier 9, camera 5, described light source, light barrier, camera are successively set on the light tool frame, and the shooting width dimensions basically identical of the width of described light barrier and workpiece being shot (ultra-violet curing optical cement 4), the width of light barrier is a bit larger tham the width of workpiece being shot, polaroid 6 is set before the described camera, black shadow shield 7 is set around the cam lens.
3, adopt reflection side illumination explicit law, with the shooting background of black background paper as the ultra-violet curing optical cement, after light reflected via soft reflector, part light was blocked by black background paper as Fig. 4,5, and part light is injected lenticule by the background paper side and held in the district.Each device comprises light source 1, light barrier 9, camera 5, reflector 10, described reflector, light source, light barrier, camera are successively set on the light tool frame 2, and described light source is invested described reflector, the shooting width dimensions basically identical of the width of described light barrier and workpiece being shot (ultra-violet curing optical cement 4), the width of light barrier 9 is a bit larger tham the width of workpiece being shot, polaroid 6 is set before the described camera, black shadow shield 7 is set around the cam lens.
Under these three kinds of light condition, optical cement is reflective can be too not strong.The dark dark profile lines of optical cement can show preferably.
Wherein, stepper motor 11 is with step angle θ=0.9 ° rotation, rotating speed 1000pps～3000pps.The shutter speed of camera 5 is more than 1/500 second, and image analytic degree is 8,000,000 pixels.9 ° of stepper motor 11 every rotations, camera is taken once, and shot image data stores in the storer, waits until subsequent treatment.
Claims (3)
1. lens dynamic outline trap setting during in-situ liquid shaping optical microlens is made, comprise light source, softbox, camera, it is characterized in that: described light source, softbox, camera being aligned successively are arranged on the runing rest, and polaroid is set before the camera, the black shadow shield is set around the cam lens; Described support is by the stepper motor driven rotary, and 9 ° of cameras of the every rotation of support are taken once.
2. lens dynamic outline trap setting during in-situ liquid shaping optical microlens as claimed in claim 1 is made, it is characterized in that: described stepper motor is with step angle θ=0.9 ° rotation, and rotating speed is 1000pps～3000pps.
3. lens dynamic outline trap setting during in-situ liquid shaping optical microlens as claimed in claim 1 is made, it is characterized in that: described camera is the CCD camera, its image analytic degree is 8,000,000 pixels.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201320070292 CN203116704U (en) | 2013-02-06 | 2013-02-06 | Dynamic lens contour capture device in in-situ liquid forming optical microlens manufacturing |
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| CN 201320070292 CN203116704U (en) | 2013-02-06 | 2013-02-06 | Dynamic lens contour capture device in in-situ liquid forming optical microlens manufacturing |
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| CN203116704U true CN203116704U (en) | 2013-08-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113481797A (en) * | 2021-06-11 | 2021-10-08 | 山西省交通建设工程质量检测中心（有限公司） | Highway subgrade road surface roughness detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113481797A (en) * | 2021-06-11 | 2021-10-08 | 山西省交通建设工程质量检测中心（有限公司） | Highway subgrade road surface roughness detection device |
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Granted publication date: 20130807 Termination date: 20140206 |