CN203053862U - Digital holographic microscopic refractive index fault imaging device - Google Patents
Digital holographic microscopic refractive index fault imaging device Download PDFInfo
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- CN203053862U CN203053862U CN 201220711022 CN201220711022U CN203053862U CN 203053862 U CN203053862 U CN 203053862U CN 201220711022 CN201220711022 CN 201220711022 CN 201220711022 U CN201220711022 U CN 201220711022U CN 203053862 U CN203053862 U CN 203053862U
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Abstract
The utility model belongs to the field of the digital holographic microscopic technology, and relates to a digital holographic microscopic refractive index fault imaging device. The digital holographic microscopic refractive index fault imaging device comprises a laser, an optical fiber output coupler, an optical fiber splitter, a lens system, a charge coupled device (CCD) camera, a sample stage and a computer. The digital holographic microscopic refractive index fault imaging device is characterized by comprising the optical fiber splitter which is connected with the optical fiber output coupler, wherein the optical fiber splitter divides the laser into reference light and object wave, the lens system comprises a reference optical lens system and an object wave lens system, the tail ends of the reference optical lens system and the object wave lens system form an off-axis holographic diagram on a recording surface of the CCD camera after being converged by a converging prism, and when the sample stage rotates, the CCD camera records a holographic diagram of each angle of a sample to be inputted to the computer. Compared with an optical coherence tomography (OCT) technology, the digital holographic microscopic refractive index fault imaging device has the advantages of high space resolution (the nm-grade resolution can be reached, and the resolution of the OCT is micrometer level), strong biological sample penetrability, high contrast ratio, field imaging, automatic focusing and the like.
Description
Technical field
The utility model belongs to the digital holographic microscopy field, relates to the micro-refractive index fault imaging device of a kind of digital hologram.
Background technology
In order to observe biological tissue, microorganism cultures and understanding material structure, people have been developed multiple tomography technology, for example X ray computer layer scanning technology (X-CT), nuclear magnetic resonance technique (MRI) and optical coherence tomography technology (OCT, Optical Coherent Tomography) etc.Wherein the OCT technology starts from twentieth century the mid-80, and it is a kind of optical sectioning imaging technology, has advantages such as safety and nondestructive, noncontact, price are lower, real time imagery, is suitable for surveying various biological tissues; And has resolution than the advantage of X-CT and MRI technology high tens even thousands of times.But self also exists defective the OCT technology, and what it was measured is certain light path depth location backscattering light signal in the sample, when index distribution is inhomogeneous on the sample path, the distortion on the depth location will occur.In addition, OCT measures reflection and the backscattering light intensity is basic imaging means, has the low shortcoming of contrast for the imaging of phase type biosome and low scattering sample.
Digital holographic microscopy is the product that digital holography and microtechnic combine, and can accurately obtain the PHASE DISTRIBUTION of thing light wave, thereby can analyze the phase information of three-dimensional surface shape or the phase type object of small items.But for the phase type sample, the digital hologram microtechnic can only be obtained the refractive index integral projection of a certain angle.
Summary of the invention
Based on digital holographic microscopic imaging technology and computer tomography (CT) principle, the utility model has been set up a kind of high accuracy number holographic microphotography refractive index fault imaging device.Device rotates based on sample, note the digital micro-analysis hologram of all angles of 180 degree scopes, utilize numerical reconstruction to obtain the phase diagram of each angle, then based on filter back projection's reconstructed image principle of CT technology, reconstruct the tomography index distribution image of sample.Compare with the OCT technology, have spatial resolution height (can reach the resolution of nm magnitude, the resolution of OCT is μ m magnitude), the biological sample penetrability is strong, contrast height, whole audience imaging, advantages such as automatic focus.Digital hologram microscopy tomography technology is not only at biomedical sector, and also has important scientific meaning and using value in many other fields such as micro-optic, photonic crystal, material analysiss.
Concrete technical scheme of the present utility model is:
The micro-refractive index fault imaging device of digital hologram, comprise laser instrument, the optical fiber output coupler, fiber optic splitter, lens combination, the CCD camera, sample platform and computing machine, it is characterized in that described fault imaging device comprises the fiber optic splitter that links to each other with the optical fiber output coupler, fiber optic splitter is divided into reference light and thing light wave with laser, lens combination comprises reference light lens combination and thing light wave lens combination, the reference light lens combination is successively by the optic fibre end clamper, neutral attenuator, the shadow shield of collimation lens and the logical light circular hole of band is formed, thing light wave lens combination is successively by the optic fibre end clamper, neutral attenuator, collimation lens, the shadow shield of the logical light circular hole of band, sample and microcobjective are formed, reference light lens combination and thing light wave lens combination end form off-axis hologram at CCD cameras record face after a beam cementing prism closes bundle, the sample platform rotates, and the hologram of CCD cameras record sample all angles is also imported computing machine.
Described sample platform is equipped with one and is used for sample wheelwork fixing and the rotation sample, and this sample wheelwork comprises one the 360 accurate rotatable platform of degree and is positioned at the sample holder of the connection branch bar at platform center, connection branch bar upper end.
The rotation step number of the described sample wheelwork of computer control, and control sample wheelwork rotates and the sequential of CCD cameras record.
Microcobjective is selected principle for use in the native system, numerical aperture must be enough big, guarantee to collect sample spectrum information as much as possible, the light path enlargement ratio must guarantee the frequency information of thing light wave is compressed to the sample frequency that satisfies CCD simultaneously, thereby can overcome the restriction of CCD registering device low resolution.
Description of drawings
Fig. 1 is the synoptic diagram of the micro-refractive index fault imaging device of digital hologram.
Perspective view under a certain angle of Fig. 2.
Wherein: 1, laser instrument; 2, optical fiber output coupler; 3,1 ' 2 fiber optic splitter; 4 and 8, optic fibre end adds and holds device; 5 and 9, neutral attenuator; 6 and 10, collimation lens; 7 and 11, narrow meshed shadow shield; 12, sample; 13, sample holder; 14, connect the branch bar; 15, accurate turntable; 16, controller; 17, beam cementing prism; 18, CCD camera; 19, computing machine; 20, microcobjective.
Embodiment
Fig. 1 is the synoptic diagram of the micro-refractive index fault imaging device of digital hologram.The laser that laser instrument 1 sends links to each other through fiber coupler 2 coupling input optical fibres and with 1 ' 2 fiber optic splitter 3, and the two-beam of output is a branch of as reference light, a branch of illumination sample crop light wave.Two output ports of fiber optic splitter are fixed with fibre holder 4 and 8.4 pairs of fiber-optic output mouths of clamper are fixed and are adjusted, and make the horizontal outgoing of outgoing beam, and central optical axis and beam cementing prism 17 optical axises form small angle.The light beam of fiber port output is a spherical light wave, forms plane light wave behind collimation lens 6 collimations, and shadow shield 7 blocks parasitic light on every side, is used as reference light wave.After with clamper 8 another fiber-optic output mouth being fixed equally and being adjusted, the horizontal outgoing of outgoing beam, central optical axis is consistent with beam cementing prism 17 optical axises.The sphere light beam of optical fiber output forms plane light wave behind collimation lens 10 collimations, shadow shield 11 blocks parasitic light on every side.Plane light wave vertical illumination sample 12 sees through the thing light wave of sample through microcobjective 20 micro-amplifications.Thing light wave and reference light wave close bundle through beam cementing prism 17, and meeting to interfere at CCD 18 record surfaces forms off-axis hologram.Neutral attenuator 5 in the system and 9 is further adjusted the ratio of reference light compound light wave, obtains the hologram of high s/n ratio, and the sample platform rotates, the hologram of CCD record sample all angles.
The sample wheelwork: one 360 accurate rotatable platform 15 center fixation of degree connect branch bar 14, connect branch bar upper end fixed sample clamper 13, use the clamper fixed sample.Rotatable platform rotates, and sample is rotated synchronously.Accurate rotatable platform links to each other with computer by controller 16, rotates step number and rotates sequential by the control software control.
The key point of sample wheelwork is the center that the sample center must place rotatable platform, otherwise can increase the complicacy of numerical value calibration.
The control software that utilization is write is controlled rotatable platform and CCD camera automatically.By the control sequential of camera and rotatable platform is set, and the rotation step number of exposure parameter and the rotatable platform of camera is set, realizes the automatic record of all angles hologram.
Another characteristics of this system are to utilize optical fiber to realize the miniaturization flexibility of system.The position of laser instrument can arbitrarily be placed, and two output terminals of optical fiber are fixed with clamper, clamper adjustment beam outbound course.
Microcobjective is selected principle for use in the system, numerical aperture must be enough big, guarantee to collect sample spectrum information as much as possible, the light path enlargement ratio must guarantee the frequency information of thing light wave is compressed to the sample frequency that satisfies CCD simultaneously, thereby can overcome the restriction of CCD registering device low resolution.
The micro-refractive index tomography technology of digital hologram is actual to be exactly that the refractive index integration data that comprises of the Wave-front phase information of utilizing filtered back projection's reconstruction technique that digital holographic microscopic imaging is obtained solves one by one, to obtain the distributed in three dimensions of sample interior refractive index, obtain other three-dimensional information then.Under each angle, record a frame off-axis digital holographic microphotography hologram, by numerical reconstruction, phase place solution parcel, phase automatic compensating computing, obtain the refractive index integral projection under each angle.
As Fig. 2, representative original image to be rebuild in shadow region among the figure, original image yet to be built is turned to by discrete
N=
k*
l*
hIndividual pixel, composition comprises
NThe one-dimension array of row [
x 1 ,
x 2 ...,
x N ], establish projection ray sum (comprising all angles) and be
M, then
iThe projecting integral of bar ray can be expressed as:
In the formula
p i Be
iThe refractive index projection value of bar ray is to measure given value;
w I, j Be weighted value, expression the
jIndividual pixel is to
iThe contribution of bar ray is from assignment;
x j Be
jThe refractive index value to be measured of individual pixel.
In data completely under the prerequisite, can utilize the filter back-projection algorithm that is inversely transformed into the basis with radon to rebuild the index distribution of sample interior, mathematical description is suc as formula (2):
In the formula,
Represent function yet to be built
N (r, q) jThe projection of direction,
lThe expression projected length and
jIt is projecting direction.
This programme has made up system and device as shown in Figure 1, and laser instrument adopts semi-conductor solid laser, wavelength 532nm in the device.Fiber-optic output adopts the FC/APC interface, adopts optical fiber method door screen to do fibre-optical fixator, utilizes the structural adjustment of the mechanical adjustment up and down output light direction of method door screen.Adopt the electronic accurate rotatable platform of 360 degree, connect the branch bar in the rotatable platform center fixation, branch bar upper end connects sample holder.Sample is single-mode fiber in this example.Adopt 0.25/10 the micro-amplification of microcobjective, enlargement ratio is 10.Sample is imaged on the CCD plane.Computer control CCD camera and rotatable platform are realized recording a width of cloth hologram automatically every 1 degree in the 180 degree scopes.
Claims (3)
1. the micro-refractive index fault imaging device of digital hologram, comprise laser instrument, the optical fiber output coupler, fiber optic splitter, lens combination, the CCD camera, sample platform and computing machine, it is characterized in that described fault imaging device comprises the fiber optic splitter that links to each other with the optical fiber output coupler, fiber optic splitter is divided into reference light and thing light wave with laser, lens combination comprises reference light lens combination and thing light wave lens combination, the reference light lens combination is successively by the optic fibre end clamper, neutral attenuator, the shadow shield of collimation lens and the logical light circular hole of band is formed, thing light wave lens combination is successively by the optic fibre end clamper, neutral attenuator, collimation lens, the shadow shield of the logical light circular hole of band, sample and microcobjective are formed, reference light lens combination and thing light wave lens combination end form off-axis hologram at CCD cameras record face after a beam cementing prism closes bundle, the sample platform rotates, and the hologram of CCD cameras record sample all angles is also imported computing machine.
2. the micro-refractive index fault imaging device of digital hologram according to claim 1, it is characterized in that described sample platform is equipped with one and is used for sample wheelwork fixing and the rotation sample, this sample wheelwork comprises one the 360 accurate rotatable platform of degree and is positioned at the sample holder of the connection branch bar at platform center, connection branch bar upper end.
3. the micro-refractive index fault imaging device of digital hologram according to claim 1 and 2 is characterized in that the rotation step number of the described sample wheelwork of computer control, and controls the sequential of the rotation of sample wheelwork and CCD cameras record.
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| CN 201220711022 CN203053862U (en) | 2012-12-20 | 2012-12-20 | Digital holographic microscopic refractive index fault imaging device |
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| CN 201220711022 CN203053862U (en) | 2012-12-20 | 2012-12-20 | Digital holographic microscopic refractive index fault imaging device |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390722A (en) * | 2014-11-14 | 2015-03-04 | 北京航空航天大学 | Optical fiber conduction-based digital holographic tomographic three-dimensional temperature measuring device |
| CN104819961A (en) * | 2015-04-23 | 2015-08-05 | 上海大学 | Digital holography system for carrying out online non-destructive measurement on refractive index of special optical fiber |
| CN105159043A (en) * | 2015-09-29 | 2015-12-16 | 南京理工大学 | Reflective digital holographic microscopic imaging device based on telecentric optical structure |
| CN105242512A (en) * | 2015-09-29 | 2016-01-13 | 南京理工大学 | Telecentric optical structure-based transmission-type digital holographic microscopic imaging device |
| CN106651964A (en) * | 2017-02-28 | 2017-05-10 | 嘉兴锐视智能科技有限公司 | Calibration system and calibration device of laser camera |
| CN108801980A (en) * | 2018-03-30 | 2018-11-13 | 北京工业大学 | Microfluid refractive index and concentration measurement system based on digital holographic microscopy and method |
| CN110108232A (en) * | 2019-05-10 | 2019-08-09 | 南京理工大学 | A kind of three mode digital holographic microscopic imaging systems |
| CN113295632A (en) * | 2021-05-24 | 2021-08-24 | 三门峡职业技术学院 | Environmental pollution monitoring devices based on big data and artificial intelligence |
-
2012
- 2012-12-20 CN CN 201220711022 patent/CN203053862U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390722A (en) * | 2014-11-14 | 2015-03-04 | 北京航空航天大学 | Optical fiber conduction-based digital holographic tomographic three-dimensional temperature measuring device |
| CN104819961A (en) * | 2015-04-23 | 2015-08-05 | 上海大学 | Digital holography system for carrying out online non-destructive measurement on refractive index of special optical fiber |
| CN104819961B (en) * | 2015-04-23 | 2019-02-22 | 上海大学 | A kind of digital hologram system of online nondestructive measurement special optical fiber refractive index |
| CN105159043A (en) * | 2015-09-29 | 2015-12-16 | 南京理工大学 | Reflective digital holographic microscopic imaging device based on telecentric optical structure |
| CN105242512A (en) * | 2015-09-29 | 2016-01-13 | 南京理工大学 | Telecentric optical structure-based transmission-type digital holographic microscopic imaging device |
| CN106651964A (en) * | 2017-02-28 | 2017-05-10 | 嘉兴锐视智能科技有限公司 | Calibration system and calibration device of laser camera |
| CN108801980A (en) * | 2018-03-30 | 2018-11-13 | 北京工业大学 | Microfluid refractive index and concentration measurement system based on digital holographic microscopy and method |
| CN110108232A (en) * | 2019-05-10 | 2019-08-09 | 南京理工大学 | A kind of three mode digital holographic microscopic imaging systems |
| CN110108232B (en) * | 2019-05-10 | 2020-12-04 | 南京理工大学 | Three-mode digital holographic microscopic imaging system |
| CN113295632A (en) * | 2021-05-24 | 2021-08-24 | 三门峡职业技术学院 | Environmental pollution monitoring devices based on big data and artificial intelligence |
| CN113295632B (en) * | 2021-05-24 | 2023-05-16 | 三门峡职业技术学院 | Environmental pollution monitoring device based on big data and artificial intelligence |
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Granted publication date: 20130710 Termination date: 20141220 |
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