CN202898395U - Controllable hydraulic bioreactor for tissue engineering - Google Patents
Controllable hydraulic bioreactor for tissue engineering Download PDFInfo
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- CN202898395U CN202898395U CN 201220540205 CN201220540205U CN202898395U CN 202898395 U CN202898395 U CN 202898395U CN 201220540205 CN201220540205 CN 201220540205 CN 201220540205 U CN201220540205 U CN 201220540205U CN 202898395 U CN202898395 U CN 202898395U
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- top cover
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Abstract
The utility model discloses a controllable hydraulic bioreactor for tissue engineering, comprising a tank body and a top cover. The bioreactor is characterized in that a controllable hydraulic system is arranged at the upper end of the top cover, a circulating filling system is arranged at the lower part of the tank body, a flexible diaphragm which divides a cavity formed by the tank body and the top cover into a culturing chamber and a pressurizing chamber is arranged inside the tank body, and a multi-hole shelf plate is arranged at the lower part of the culturing chamber. A hydraulic pressurizing manner and a controllable pressure relief technology are adopted by the bioreactor disclosed by the utility model so that real-time and precise regulation and control on key parameters such as pressure stimulation strength, frequency and action time can be achieved to realize the switch among a plurality of stimulation modes; and a culture fluid inlet and a culture fluid outlet are added in a culture system to realize circulating filling culture so that complexity of basic operations such as fluid changing in an engineering cartilage construction process is greatly simplified, and pollution possibility caused by repeated uncovering operations is reduced.
Description
Technical field
The utility model relates to a kind of organizational project bio-reactor, particularly relates to a kind of controlled hydraulic bio-reactor that is used for organizational project that possesses independent pressurization cavity.
Background technology
The auricular defect that the factor such as congenital microtia, wound causes is very common in plastic surgery, but it is large to take from body bone carving reparation at quarter wound, utilizes the organizational engineering method will become important medical new departure of repairing auricular defect at the cartilage that external structure goes out to have accurate auricle form.But at present people's auricular cartilage mechanical property of external structure is relatively poor, does not still reach the clinical requirement of rebuilding the auricle morphological stability after being implanted to subcutis.Therefore must when external structure, improve the mechanical property of through engineering approaches cartilage.
Mechanical stimulation can significantly improve through engineering approaches cartilage mechanical property by approach such as enhancing chondrocyte proliferation, differentiation, extracellular matrix synthesize.Present most widely used bio-reactor mainly contains two kinds---shearing force and stress bio-reactor.Yet these two kinds of reactors are mainly take anisobaric application of force pattern as main, because application of force direction is single, can cause cartilage non-stress face to lack corresponding mechanical stimulation, thereby cause external structure cartilage mechanical property heterogeneity, do not reach the cartilage (such as auricular cartilage) of large volume, specific form for mechanical property and the accurately requirement of shape.Therefore, develop a can on all directions, evenly exerting pressure, and can not cause the bio-reactor of cartilage metamorphosis, most important to external structure through engineering approaches cartilage.
The utility model content
The technical problems to be solved in the utility model is to overcome defects, and a kind of controlled hydraulic bio-reactor that is used for organizational project that can evenly exert pressure to culture on all directions is provided.
For addressing the above problem, the technical scheme that the utility model adopts is:
A kind of controlled hydraulic bio-reactor for organizational project comprises tank body, is provided with top cover on the tank body, and it is characterized in that: the upper end of described top cover is provided with the controlled hydraulic system, and the bottom of described tank body is provided with the circumfusion system;
Be provided with flexible partition in the described tank body, flexible partition is separated into culture chamber and pressurizing chamber with the cavity of tank body and top cover formation;
The bottom of described culture chamber is provided with the porous shelf, and the porous shelf is in order to place culture;
Described controlled hydraulic system comprises that an end runs through and is installed in pipeline on the top cover, and the other end of pipeline is equipped with hydraulic efficiency plant;
Described circumfusion system comprises two pipelines that are installed in respectively on the tank body, and two pipelines are the upper-lower position relation; The pipeline that is positioned at the top is drain pipe, and drain pipe is connected with device for storing liquid, and the pipeline that is positioned at the below is liquid-inlet pipe, and liquid-inlet pipe is connected with device for storing liquid; Be provided with power set between described liquid-inlet pipe and the device for storing liquid.
As a kind of improvement:
Also be provided with two pipelines on the described top cover, the lower end of pipeline is run through and is installed on the top cover, and the upper end is separately installed with safety valve and pressure release valve.
As further improvement:
The bottom of described tank body is provided with pipeline, and an end of pipeline runs through and is installed on the tank body, and the other end is equipped with tensimeter.
On the described liquid-inlet pipe control valve is installed; On the described drain pipe control valve is installed.
As further improving:
Described power set is peristaltic pump, and peristaltic pump is communicated with liquid storage bottle; Described hydraulic efficiency plant is gas cylinder.
Described tank body is fixed by bolt and top cover.
Owing to adopted technique scheme, compared with prior art, the utility model has the advantages that: its adopts hydraulic pressurization mode, can provide uniform Pressure stimulation to each thrust surface of the culture in the reactor and can not cause the mechanical deformation of culture; Utilize flexible partition that compression system is separated with culture systems, on the basis that does not affect the pressurization effect, can also effectively avoid pollutent and gas under pressure to enter culture system; Adopt gas pressurization mode and controlled release technology, can realize real-time, precision control to key parameters such as Pressure stimulation intensity, frequency, action times, realize the conversion of multiple stimulus modality; In culture systems, introduce the nutrient solution import and export and cultivate to realize circumfusion, thereby greatly simplify the complexity of changing the elementary operations such as liquid in the through engineering approaches cartilage building process, and reduce the possibility that the operation that repeats to uncap causes pollution.
The utility model is described in further detail below in conjunction with the drawings and specific embodiments.
Description of drawings
Fig. 1 is the structural representation of a kind of embodiment of the utility model embodiment.
Among the figure: the 1-tank body; The 2-top cover; The 3-bolt; The 4-pipeline; The 5-safety valve; The 6-pipeline; The 7-pressure release valve; The 8-pipeline; The 9-hydraulic bottle; The 10-drain pipe; The 11-control valve; The 12-liquid storage bottle; The 13-tensimeter; The 14-pipeline; 15-porous shelf; The 16-flexible partition; The 17-culture chamber; The 18-pressurizing chamber; The 19-liquid-inlet pipe; The 20-liquid feed valve; The 21-peristaltic pump.
Embodiment
Embodiment:
As shown in Figure 1, a kind of controlled hydraulic bio-reactor for organizational project comprises tank body 1, is provided with top cover 2 on the tank body 1, and the upper end of described top cover 2 is provided with the controlled hydraulic system, and the bottom of described tank body 1 is provided with the circumfusion system.Be arranged on the culture in the tank body, between the pressurization stimulation period, provide required hydraulic pressure to stimulate by the controlled hydraulic system.In the training period, the circumfusion system provides fresh medium and is swapped out old nutrient solution for culture.
Be provided with flexible partition 16 in the described tank body 1, flexible partition 16 is separated into culture chamber 17 and pressurizing chamber 18 with the cavity of tank body 1 and top cover 2 formation.Flexible partition 16 also can be replaced by other identical spacers of function, so every substituent with flexible isolating effect all belongs in the protection domain of the present utility model.The bottom of culture chamber 17 is provided with porous shelf 15, and porous shelf 15 is in order to place culture.
In the present embodiment, the controlled hydraulic system comprises that an end runs through and is installed in pipeline 8 on the top cover 2, and the other end of pipeline 8 is equipped with hydraulic efficiency plant.Hydraulic efficiency plant, pipeline 8 and flexible partition 16 are realized the controllability of hydraulic pressure jointly.Wherein, consider practical situation, hydraulic efficiency plant is selected the gas cylinder 9 of 15Mpa pressurized air specification.
In addition, also be provided with two pipelines 4,6 on the top cover 2, pipeline 4,6 lower end are run through and are installed on the top cover 2, and the upper end is separately installed with safety valve 5 and pressure release valve 7.Wherein the opening pressure of safety valve 5 is 10Mpa, and pressure release valve 7 is gas pressure reducer.
Also have, tank body 1 can be fixing with top cover 2 by bolt 3.Consider the factor of actually operating aspect, the tank body material is the 316L stainless steel, intracavity diameter 100mm, high 100mm, thick 7mm, the thick 40mm of tank flange; The top cover material is the 316L stainless steel.Bolt 3 materials between tank body and the top cover are 304 stainless steels, nut specification M22.
In the present embodiment, the circumfusion system comprises two pipelines that are installed in respectively on the tank body, and two pipelines are the upper-lower position relation.The pipeline that is positioned at the top is drain pipe 10, and drain pipe 10 is connected with device for storing liquid, and the pipeline that is positioned at the below is liquid-inlet pipe 19, and liquid-inlet pipe 19 is connected with device for storing liquid.Be provided with power set between described liquid-inlet pipe 19 and the device for storing liquid.Consider practical situation, device for storing liquid is liquid storage bottle 12.Power set is peristaltic pump 21, and peristaltic pump 21 is communicated with liquid storage bottle.In addition, consider actual needs, each pipeline in the circumfusion system is made by silicone rubber tube.
In addition, the bottom of tank body 1 is provided with pipeline, and an end of pipeline runs through and is installed on the tank body 1, and the other end is equipped with tensimeter 13.What according to actual needs, tensimeter 13 adopted is 316L stainless steel pressure table.Control valve 20 is installed on the described liquid-inlet pipe, control valve 11 is installed on the described drain pipe.Pipeline between the above-mentioned all parts is all made by silicone tube.
Implementation step of the present utility model is as follows:
Step 1: the control valve on the liquid-inlet pipe and the control valve on the drain pipe are closed, culture placed the porous shelf and pour cell culture fluid in the culture chamber make liquid level cover culture, flexible partition is placed between culture chamber and the pressurizing chamber and with bolt top cover closely is fixedly connected with tank body.
Step 2: open control valve, the control valve on the drain pipe and peristaltic pump on the liquid-inlet pipe, nutrient solution in the liquid storage bottle is fed into culture chamber, treat that drain pipe has nutrient solution to flow out, when namely flexible partition has been close to the nutrient solution surface, closeall control valve and peristaltic pump.
Step 3: close pressure release valve, open hydraulic bottle, the output pressure of regulator solution pressure bottle is adjusted to 5Mpa, and the tensimeter on the tank body shows that the culture chamber internal pressure is 5MPa at this moment, pressurize 30 minutes;
Step 4: pressurize is closed hydraulic bottle after finishing, and opens pressure release valve, and the output pressure of adjusting pressure release valve is to 0.1MPa, and after for some time, the pressure in the culture chamber reduces to 0.
Step 5: the hydraulic pressure stimulating course is opened control valve, the control valve on the drain pipe and peristaltic pump on the liquid-inlet pipe after finishing, and realizes the reactant in the reactor is realized the circumfusion cultivation.
Above-described only is preferred implementation of the present utility model; should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the utility model creation design; can also make some distortion and improvement, these all belong to protection domain of the present utility model.
Claims (6)
1. the controlled hydraulic bio-reactor that is used for organizational project, comprise tank body (1), be provided with top cover (2) on the tank body (1), it is characterized in that: the upper end of described top cover (2) is provided with the controlled hydraulic system, and the bottom of described tank body (1) is provided with the circumfusion system;
Be provided with flexible partition (16) in the described tank body, flexible partition (16) is separated into culture chamber (17) and pressurizing chamber (18) with the cavity of tank body (1) and top cover (2) formation;
The bottom of described culture chamber is provided with porous shelf (15), and porous shelf (15) is in order to place culture;
Described controlled hydraulic system comprises that an end runs through and is installed in pipeline (8) on the top cover (2), and the other end of pipeline (8) is equipped with hydraulic efficiency plant;
Described circumfusion system comprises two pipelines that are installed in respectively on the tank body, and two pipelines are the upper-lower position relation; The pipeline that is positioned at the top is drain pipe (10), and drain pipe (10) is connected with device for storing liquid, and the pipeline that is positioned at the below is liquid-inlet pipe (19), and liquid-inlet pipe (19) is connected with device for storing liquid; Be provided with power set between described liquid-inlet pipe (19) and the device for storing liquid.
2. the controlled hydraulic bio-reactor for organizational project according to claim 1, it is characterized in that: also be provided with two pipelines (4,6) on the described top cover, the lower end of pipeline (4,6) is run through and is installed on the top cover, and the upper end is separately installed with safety valve (5) and pressure release valve (7).
3. the controlled hydraulic bio-reactor for organizational project according to claim 2, it is characterized in that: the bottom of described tank body (1) is provided with pipeline (14), one end of pipeline runs through and is installed on the tank body (1), and the other end is equipped with tensimeter (13).
4. the controlled hydraulic bio-reactor for organizational project according to claim 3 is characterized in that: control valve (20) is installed on the described liquid-inlet pipe (19); Control valve (11) is installed on the described drain pipe (10).
5. the controlled hydraulic bio-reactor for organizational project according to claim 4, it is characterized in that: described hydraulic efficiency plant is gas cylinder (9); Described power set is peristaltic pump (21), and peristaltic pump (21) is communicated with liquid storage bottle (12).
6. the described controlled hydraulic bio-reactor for organizational project of any one according to claim 1-5 is characterized in that: described tank body (1) is fixing by bolt (3) and top cover (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220540205 CN202898395U (en) | 2012-10-22 | 2012-10-22 | Controllable hydraulic bioreactor for tissue engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220540205 CN202898395U (en) | 2012-10-22 | 2012-10-22 | Controllable hydraulic bioreactor for tissue engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202898395U true CN202898395U (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220540205 Expired - Fee Related CN202898395U (en) | 2012-10-22 | 2012-10-22 | Controllable hydraulic bioreactor for tissue engineering |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899250A (en) * | 2012-10-22 | 2013-01-30 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
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2012
- 2012-10-22 CN CN 201220540205 patent/CN202898395U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899250A (en) * | 2012-10-22 | 2013-01-30 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
| WO2014063574A1 (en) * | 2012-10-22 | 2014-05-01 | 湖南大学 | Controllable hydraulic bioreactor used in tissue engineering |
| CN102899250B (en) * | 2012-10-22 | 2014-12-03 | 湖南大学 | Tissue engineering controllable hydraulic bioreactor |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130424 Termination date: 20141022 |
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| EXPY | Termination of patent right or utility model |