CN202837309U - Novel automatic western blot analyzer - Google Patents
Novel automatic western blot analyzer Download PDFInfo
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- CN202837309U CN202837309U CN201220457942.9U CN201220457942U CN202837309U CN 202837309 U CN202837309 U CN 202837309U CN 201220457942 U CN201220457942 U CN 201220457942U CN 202837309 U CN202837309 U CN 202837309U
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Abstract
The utility model relates to a novel automatic western blot analyzer. In the prior art, the problem that a reagent residues in a pipeline is serious, and is prone to forming liquid drops in a liquid-adding nozzle, and causing testing pollutions. To solve the problem, the novel automatic western blot analyzer comprises an incubation shaking bed, a plurality of liquid-adding arms, a plurality of liquid-absorbing arms, a rotary motor, a shaking motor, a vacuum pump and an early warning component, and is characterized in that one ends of the plurality of the liquid-adding arms are fixedly arranged on the outer edge of the incubation shaking bed in a surrounding mode, the other ends of the plurality of the liquid-adding arms are provided with liquid-adding nozzles, a liquid-adding peristaltic pump is arranged below a bottle frame, and a reversing controlling device is arranged at the liquid-adding peristaltic pump. A plurality of independent shaking boards are arranged on the incubation shaking bed, and a slot board is arranged on each shaking board. The incubation shaking bed is disc-shaped, and the shaking boards are fan-shaped. The novel automatic western blot analyzer reduces residue amount of reagents in a maximum degree, solves the problem that liquid drops are prone to hanging on the liquid-adding nozzles, and ensures a test to be performed reliably.
Description
Technical field
The utility model relates to a kind of protein blot experiment device, particularly a kind of new type auto westem blot analyzer.
Background technology
Detected by Western blot is a kind of effective ways of identifying antigen by specific antibody, the method is a kind of new immune biochemical technology that grows up in gel electrophoresis and solid-phase immunoassay technical foundation, whole process comprises: protein electrophorese separates, electrotransfer, immunology detection and input four parts.Immune detection partly comprises following various motion in the existing protein blot experiment: liquid feeding, imbibition, hatch, the sequence of operations such as washing, action step is rigorous and complicated.
At present, although existing multiple protein trace instrument is used for solving the inconvenience that manual operations brings.But existing this quasi-instrument is controlled in the ducted residual shortage of dosing reagent, owing to moving to carry out the liquid feeding operating position fixing by the driven by motor liquid adding arm, cause pipeline to chew the mobile very large length that needs with liquid feeding, reagent residual problem in pipeline is serious, particularly domestic Immunoassay Product comes from import more, supporting deal is strictly distributed in the person-portion ratio, does not have unnecessary allowance.In addition, traditional instrument is in the dosing process, and reagent is easily chewed the formation drop at liquid feeding, and then is subject to the vibration influence of moving component and drops, and causes test to pollute.
The utility model content
The technical problems to be solved in the utility model is the defects that how to overcome prior art, and a kind of new type auto westem blot analyzer is provided.
For solving the problems of the technologies described above, this new type auto westem blot analyzer, comprise incubation shaking table, a plurality of liquid adding arm, liquid absorbing arm, rotary electric machine, shake motor, vacuum pump and early warning parts, it is characterized in that: described a plurality of liquid adding arm one end ring shapes are around the outward flange that is fixedly installed in incubation shaking table, the other end is provided with liquid feeding and chews, described liquid feeding is chewed by pipeline and is connected with reagent bottle, described reagent bottle is located on the bottle stand, described bottle stand has the liquid feeding peristaltic pump, and described liquid feeding peristaltic pump place is provided with reversal control device; Described incubation shaking table is provided with a plurality of independently rockers, places a frid on each rocker.
The length of dosing pipeline is farthest shortened in so design, reduces residual in pipeline of reagent; Solve liquid feeding and chewed the problem of easy extension liquid pearl, avoided test to pollute.
In addition, described vacuum pump is used for waste liquid bottle is produced negative pressure, and waste liquid bottle and the imbibition on the liquid absorbing arm are chewed to be connected by pipeline and carried out negative-pressure liquid suction, and liquid absorbing arm is subjected to Electric Machine Control, the height displacement that the realization imbibition is chewed.In addition, described incubation shaking table is connected by gear band with rotary electric machine, realizes the rotational positioning of frid.
As optimization, described incubation shaking table is collar plate shape.So design, the frid location that can realize dosing and imbibition step.
As optimization, described rocker is fan-shaped.So design can adapt to the test of different people umber amount, and maximum people's umber that can carry out is about two times of traditional instrument.
In addition, after test was finished, this new type auto westem blot analyzer built-in system provided the function that the reagent in the charging line can be back to reagent bottle to select.
This new type auto westem blot analyzer, the experiment capacity is large, the sample person-portion quantity that can test simultaneously is many, reduce to greatest extent the residual quantity of reagent in the pipeline, reduce the waste of resource, solve simultaneously liquid feeding and chew the problem of easy extension drop, can prevent effectively that the test that causes owing to the instrument self reason from polluting, warranty test carries out reliably.
Description of drawings
Below in conjunction with accompanying drawing this new type auto westem blot analyzer is described further:
Fig. 1 is the perspective view of this new type auto westem blot analyzer;
Fig. 2 is the plan structure schematic diagram of this new type auto westem blot analyzer;
Fig. 3 is the left TV structure schematic diagram of this new type auto westem blot analyzer;
Fig. 4 is the right TV structure schematic diagram of this new type auto westem blot analyzer;
Fig. 5 is the perspective view of the incubation shaking table module of this new type auto westem blot analyzer.
Among the figure: 1 chews, 6 is that rotational positioning optocoupler, 12 is for shaking motor, 13 for shaking the location optocoupler for rotary electric machine, 11 for imbibition motor, 10 for vacuum pump, 9 for liquid absorbing arm, 8 for liquid feeding peristaltic pump, 7 for liquid feeding for liquid adding arm, 5 for frid, 4 for rocker, 3 for incubation shaking table, 2.
Embodiment
Embodiment one: shown in Fig. 1-4, this new type auto westem blot analyzer, comprise incubation shaking table 1, eight liquid adding arms 4, liquid absorbing arm 7, rotary electric machines 10, shake motor 12, vacuum pump 8 and early warning parts, it is characterized in that: described eight liquid adding arms, 4 one end ring shapes are around the outward flange that is fixedly installed in incubation shaking table 1, the other end is provided with liquid feeding and chews 5, described liquid feeding is chewed 5 and is connected with reagent bottle by pipeline, described reagent bottle is located on the bottle stand, described bottle stand has liquid feeding peristaltic pump 6, and described liquid feeding peristaltic pump 6 places are provided with reversal control device; Described incubation shaking table 1 is provided with 12 independently rockers 2, places a frid 3 on each rocker 2.
Described incubation shaking table 1 is collar plate shape; Described rocker 2 is fan-shaped.
During use, the utility model has shared 6, one liquid feeding peristaltic pumps of eight liquid feeding peristaltic pumps 6 and has connected a liquid adding arm 4 and a reagent bottle, forms a liquid feeding passage, forms altogether eight liquid feeding passage availability tests and uses.By default, the liquid feeding range is 100uL to 3000uL to liquid volume added, can satisfy different test demands by the user.Reversal control device is added in control at liquid feeding peristaltic pump 6, after each liquid feeding action is finished, automatically controls liquid feeding peristaltic pump 6 and carries out the counter-rotating of short time, carries out gobbet and refluxes, and is used for solving liquid feeding and chews the problem that easily hangs with the liquid pearl on 5.The imbibition passage is comprised of liquid absorbing arm 7, vacuum pump 8 and waste liquid bottle, one end of liquid absorbing arm 7 maintains static, the other end is with the 9 control liftings of imbibition motor, imbibition is chewed to fit reliably the bottom, groove position of frid 3, waste liquid is drawn totally, and the air intake of vacuum pump 8 connects the gas outlet of waste liquid bottle, and the air intake of waste liquid bottle connection imbibition is chewed, make waste liquid bottle produce negative pressure by software control vacuum pump 8 work, and chew by imbibition waste liquid is drawn in the waste liquid bottle.The imbibition time can be passed through default by the user, can be corresponding with the setting of liquid volume added, be satisfied with different test demands, and save simultaneously the imbibition time.
As shown in Figure 5, incubation shaking table 1 comprises 12 fan-shaped rockers 2, and to placing 12 frids 3, each frid 3 respectively comprises four reagent troughs, altogether can carry out simultaneously the test of 48 person-portions; One end of rocker 2 is fixed, and the other end turns the lifting of eccentric wheel control push rod by the mechanical connection push rod by shaking motor 12 connections, realizes shaking hatching, and shakes speed and is divided into Three Estate, can be by the user by system's setting.In addition, the overall shape of incubation shaking table 1 is circular turntable structure, provides power by rotary electric machine 10, in conjunction with rotational positioning optocoupler 11, can make each reagent trough accurately aim at liquid feeding chew 5 and imbibition chew, in order to carry out liquid feeding and imbibition step.
In addition, the utlity model has alarm function, the software control buzzing is reported to the police, and carries out alarm when needs carry out manual ancillary test action, hatches residue in the time of one minute at incubation shaking table 1, and the prompting user is careful the test process.
The utility model includes but not limited to above-mentioned embodiment, and any product that meets the description of these claims all falls within the protection domain of the present utility model.
Claims (3)
1. new type auto westem blot analyzer, comprise incubation shaking table, a plurality of liquid adding arm, liquid absorbing arm, rotary electric machine, shake motor, vacuum pump and early warning parts, it is characterized in that: described a plurality of liquid adding arm one end ring shapes are around the outward flange that is fixedly installed in incubation shaking table, the other end is provided with liquid feeding and chews, described liquid feeding is chewed by pipeline and is connected with reagent bottle, described reagent bottle is located on the bottle stand, and described bottle stand has the liquid feeding peristaltic pump, and described liquid feeding peristaltic pump place is provided with reversal control device; Described incubation shaking table is provided with a plurality of independently rockers, places a frid on each rocker.
2. new type auto westem blot analyzer as claimed in claim 1, it is characterized in that: described incubation shaking table is collar plate shape.
3. new type auto westem blot analyzer as claimed in claim 2, it is characterized in that: described rocker is fan-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220457942.9U CN202837309U (en) | 2012-09-11 | 2012-09-11 | Novel automatic western blot analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201220457942.9U CN202837309U (en) | 2012-09-11 | 2012-09-11 | Novel automatic western blot analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202837309U true CN202837309U (en) | 2013-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201220457942.9U Expired - Lifetime CN202837309U (en) | 2012-09-11 | 2012-09-11 | Novel automatic western blot analyzer |
Country Status (1)
| Country | Link |
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| CN (1) | CN202837309U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954785A (en) * | 2014-03-20 | 2014-07-30 | 欧蒙医学诊断（中国）有限公司 | Liquid system of western-blot analyzer |
| CN103983795A (en) * | 2014-03-25 | 2014-08-13 | 深圳市爱康生物科技有限公司 | Blood card puncturing, sampling and incubation apparatus |
| CN104007270A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis apparatus |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN108226463A (en) * | 2018-01-19 | 2018-06-29 | 深圳市亚辉龙生物科技股份有限公司 | Blending incubation device and method |
| CN110631883A (en) * | 2019-08-23 | 2019-12-31 | 山东滨州拓普化工工程有限公司 | High-efficient automatic dyeing appearance |
-
2012
- 2012-09-11 CN CN201220457942.9U patent/CN202837309U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954785A (en) * | 2014-03-20 | 2014-07-30 | 欧蒙医学诊断（中国）有限公司 | Liquid system of western-blot analyzer |
| CN104007270A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis apparatus |
| CN103954785B (en) * | 2014-03-20 | 2016-06-29 | 欧蒙医学诊断（中国）有限公司 | A kind of liquid system of westem blot analyzer |
| CN104007270B (en) * | 2014-03-20 | 2016-08-17 | 欧蒙医学诊断（中国）有限公司 | A kind of analytical equipment of biological detection |
| CN103983795A (en) * | 2014-03-25 | 2014-08-13 | 深圳市爱康生物科技有限公司 | Blood card puncturing, sampling and incubation apparatus |
| CN103983795B (en) * | 2014-03-25 | 2015-08-26 | 深圳市爱康生物科技有限公司 | The puncture of blood card, application of sample and incubating device |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN108226463A (en) * | 2018-01-19 | 2018-06-29 | 深圳市亚辉龙生物科技股份有限公司 | Blending incubation device and method |
| CN110631883A (en) * | 2019-08-23 | 2019-12-31 | 山东滨州拓普化工工程有限公司 | High-efficient automatic dyeing appearance |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130327 |