CN202705367U - Electrochemical gene detecting system - Google Patents
Electrochemical gene detecting system Download PDFInfo
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- CN202705367U CN202705367U CN 201120569195 CN201120569195U CN202705367U CN 202705367 U CN202705367 U CN 202705367U CN 201120569195 CN201120569195 CN 201120569195 CN 201120569195 U CN201120569195 U CN 201120569195U CN 202705367 U CN202705367 U CN 202705367U
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Abstract
The utility model provides an electrochemical gene detecting system which comprises an upper processing terminal and a detecting terminal. The detecting terminal comprises a gene detector and a controller. The gene detector is used for marking for molecules by means of electrochemical technology according to hybridization reaction and scanning marked detecting signals by means of alternating current voltammetry. The controller controls the gene detector to achieve gene detection, receives scanning data of the gene detector, carries out result analysis, and sends analyzed results to the upper processing terminal. The upper processing terminal adjusts the controller in real time by sending control instruments. According to the electrochemical gene detecting system, the detecting process is controlled by means of high-precision instruments, the detecting speed and efficiency are greatly improved, detecting cost and labor sources are effectively saved and high-speed, accurate and precise deoxyribonucleic acid (DNA) detection is achieved.
Description
Technical field
The utility model relates to the technique of gene detection field, particularly relates to a kind of electrochemical gene detection system.
Background technology
1998 the end of the year AAAS classify the electrochemical gene sensor technology one of as large progress in 1998 annual natural science fields ten, it serves to show its meaning in the history of science.It with its can be simultaneously, analyze the ability of thousands of genomic informations quickly and accurately and demonstrated huge power.These are used and mainly comprise genetic expression detection, sudden change detection, genome polymorphism analysis and the aspects such as gene library mapping and sequencing by hybridization.
Adopt the change of electrochemical gene sensor gene expression detection can save a large amount of manpower and materials and financial resources, former, scientist's change situation that a plurality of genes are observed in a large amount of experiment of repetition of having to, if it almost is inconceivable using conventional methods the change of thousands of genes in the research cell, because must extraction at first extract the nucleic acid of cell, and want abundant to satisfy the needs of Northern hybridization, then each probe of mark carries out respectively hybridization check again.Adopt electrochemical gene sensor that thousands of times of ground of workload are reduced, utilize electrochemical gene sensor to detect simultaneously the function of 6000 genes in the yeast, the expression change of 8600 genes among the human fibroblasts has then detected to achievement in the Patrick Brown of Stanford University leader's scientific research group.
Electrochemical gene sensor also can be used for gene sequencing, the present U.S. Human Genome Project is being greatly developed this technology and is being striven for substituting present automatic sequencing, compare with automatic sequencing with existing manual order-checking, the electrochemical gene sensor order-checking can be saved a large amount of reagent and instrument loss.People more successfully comprise that to multiple biology Arabidopis thaliana, yeast and people's genomic expression situation is studied in the research that genetic expression detects.Facts have proved that the electrochemical gene sensor technology also can be used for the detection of nucleic acid mutation and the analysis of genome polymorphism, the sudden change that reaches 98% grade with conventional sequencing result consistence detects, to evaluation, mapping and the somatotype of single nucleotide polymorphisms among whole genome, the research of human mitochondria gene group polymorphism etc.
Biosensor is combined with chip technology, by the verified single base mutation that can detect gene of strength of electric field in change probe array zone, thereby by the order of determining overlapping clone Yeast genome is mapped.
Sequencing by hybridization is another important application of electrochemical gene sensor technology.This sequencing technologies a kind of efficient feasible sequence measurement of can yet be regarded as in theory, but need can derive by the hybridization of a large amount of overlap probes and molecules of interest the sequence of purpose nucleic acid molecule, so need to make a large amount of probes.The electrochemical gene sensor technology can be synthesized and fixing a large amount of nucleic acid molecule with comparalive ease, so its appearance provides the possibility of practice undoubtedly for sequencing by hybridization.
Adopt the electrochemical gene sensor technology, can greatly accelerate the job schedule of the Human Genome Project, such as be used for gene sequencing, genetic expression detects and new genetic marker such as SNP location etc., this is significant to seeking new functional gene, seek new drug target and developing new genomic medicine.Adopt that electrochemical gene sensor can carry out that workload out of the former imagination detects different plant species, different tissues, differently sickly plants, the genetic expression under the different treatment condition changes, thereby know exploitation have different purposes diagnostic kit.New drug must be tested by human safety in the experimental phase, just must observe medicine to the impact of human gene expression, owing to and not knowing that medicine works to that a kind of gene, just must the genetic expression in known all or the certain limit all be detected, adopt electrochemical gene sensor can finish rapidly and exactly this task.
But have now by electrochemical sensor and carry out the step that gene test need to be more, and higher for artificial operational requirement, the complex steps time of detecting simultaneously is longer, simultaneously the situation that a plurality of samples detect is needed a large amount of manpower and materials for needs, improved testing cost, again and, owing to being subject to the restriction of local and equipment, the very large restriction that obviously has for the processing of Multi-example, particularly for places such as hospital and laboratories, be subject to the restriction in space, too much equipment can not be installed and hold too much operator, lowered greatly the detection efficiency for sample, than and for needing in the testing process sample is shifted or operates, because a plurality of step separating treatment easily make sample or data be affected, thereby have affected detection accuracy greatly.
Summary of the invention
The purpose of this utility model is to provide a kind of easy, electrochemical gene detection system fast.
A kind of electrochemical gene detection system comprises: upper processing terminal and sense terminals;
Described sense terminals comprises: gene test device and controller, described gene test device is used for according to hybridization, adopt electrochemical techniques that molecule is carried out mark, and by the detection signal of alternating voltammetry scanning through mark, described controller is controlled described gene test device and is carried out gene test, receives the scan-data of gene test device, and carries out interpretation of result, and analytical results is sent to upper processing terminal, described upper processing terminal is with by the real-time adjustment control of sending controling instruction;
Preferably, described gene test device comprises: electrochemical gene sensor, junctor, the second connection management device and chip heating unit, molecule after described electrochemical gene sensor adopts electrochemical techniques to thing hybridization to be detected carries out mark, realize gene test, described the second connection management device is by the detection signal of alternating voltammetry scanning through mark, and according to the process of the instruction control gene test of described controller, and the temperature of controlling described chip heating unit; Described junctor is used for described electrochemical gene sensor and is connected with the chip heating unit with the second connection management device.
Preferably, described upper processing terminal comprises:
Be used for creating in treating processes the module that arranges of one or more user's setting options;
Be used for receiving in treating processes the second receiver module of the data signal of controller transmission;
Be used for the computing module processed in the data that treating processes receives the second receiver module according to user's setting option;
For the output module that transmits control signal to controller according to user's setting option in treating processes;
Be used for receiving in treating processes the first receiver module of one or more inputs of user, one or more inputs of described user are corresponding to described one or more user's setting options;
Preferably, described controller comprises: the first management link block, the first processing module and the second processing module, described the first management link block is connected with described gene test device, receive the scan-data of the second connection management device output, and carry out data interaction with described the second connection management device; Described the second processing module is carried out interpretation of result to the data of obtaining; Described the first processing module is connected with described the first management link block, is used for described gene test device is carried out control and management, the chip heating unit is controlled, and be used for external communication and connection management.
Preferably, the utility model comprises one or more sense terminals, be used for one or more things to be detected are carried out gene test, described sense terminals comprises one or more proofing units, described proofing unit comprises one or more gene test devices, be used for one or more things to be detected are carried out gene test, described chip heating unit comprises one or more chip fire ends, is connected with described one or more gene test devices.
Preferably, described junctor comprises:
The first coupling end that is connected with described electrochemical gene sensor;
Be connected with described chip heating unit, regulate the environment control end of the temperature of control electrochemical gene sensor;
The second electric signal transmission interfaces that is connected with described the second connection management device;
Described the first coupling end comprises: electrochemical gene sensor fixed bit and the first electric signal transmission interfaces that is connected with described electrochemical gene sensor, described electrochemical gene sensor fixed bit is used for fixedly electrochemical gene sensor, and described the first electric signal transmission interfaces is used for carrying out data transmission with electrochemical gene sensor;
Described the second electric signal transmission interfaces and described the first electric signal transmission interfaces interconnect, and are used for transmission of electric signals between the second connection management device and the described electrochemical gene sensor.
Preferably, described the first processing module comprises: data processing unit and temperature control unit, described data processing unit is used for described gene test device is carried out control and management, and for external communication and connection management, described temperature control unit is used for control chip heating unit, the temperature of regulating in real time electrochemical gene sensor.
Preferably, stating temperature control unit comprises: one or more temperature control components and one or more Temperature Feedback element, described one or more temperature control component corresponds respectively to one or more gene test devices, described one or more temperature feedback element corresponds respectively to one or more gene test devices, described one or more detector unit is followed according to the monitoring temperature of described one or more Temperature Feedback unit to the gene test device, respectively the chip heating unit on one or more gene test devices is controlled in real time.
Preferably, described one or more temperature control component comprises:
Comparing element is used for after the detected temperature simulation digital conversion in one or more Temperature Feedback unit, numerary signal is converted to temperature and target temperature compares;
Controlling elements, result based on the comparison according to parameter prediction temperature variations such as the amplitude of temperature variation, speed, adjusts temperature control work output in real time;
Power element according to the output manipulated variable of controlling elements, is controlled the temperature of described chip heating unit
Preferably, the second connection management device comprises: digital circuit, modular converter, low-voltage driving module and voltage acquisition reaction circuit;
The work output of the energizing voltage that described Digital Circuit Control low-voltage driving module produces, the voltage acquisition reaction circuit gathers energizing voltage, and modular converter becomes numerary signal with analog signal conversion, the controller collection comprise voltage acquisition and through the numerary signal that analog signal conversion becomes, detect, control and interpretation of result.
Compared with prior art, the utlity model has following advantage:
The technical solution of the utility model since intend to adopt each chip independently temperature be controlled to be DNA hybridization highly stable independently environment be provided, and adopt high precision instrument that checkout procedure is controlled, speed and the efficient of check have been improved greatly, and effectively saved cost and the manpower of check, reached at a high speed, accurately and accurately checked DNA.Be used for gene sequencing, compare with automatic sequencing with existing manual order-checking, can save a large amount of reagent and instrument loss.As long as whole detection hybridization and testing process be 30 minutes, and fast and easy, cheapness is compared with order-checking and to have been reduced greatly cost, realizes low-cost detection to the specific gene segment.Can carry out hybridization check to 24 chips simultaneously, high-throughout examination and the detection of carrying out disease.Whole hybridization and testing process automatization have realized left unguarded, have alleviated operator's burden, have improved efficient.
Description of drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of proofing unit in the utility model;
Fig. 3 is the structural representation of the first processing module in the utility model;
Fig. 4 is the syndeton of junctor in the utility model;
Fig. 5 is temperature controlled schematic diagram in the utility model.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail.Should be appreciated that specific embodiment described herein only is used for explaining the utility model, and be not used in restriction the utility model.
Shown in Fig. 1-4, the utility model provides a kind of electrochemical gene detection system, comprising: upper processing terminal and sense terminals;
Described sense terminals comprises: gene test device and controller, described gene test device is used for according to hybridization, adopt electrochemical techniques that molecule is carried out mark, and by the detection signal of alternating voltammetry scanning through mark, described controller is controlled described gene test device and is carried out gene test, receives the scan-data of gene test device, and carries out interpretation of result, and analytical results is sent to upper processing terminal, described upper processing terminal is with by the real-time adjustment control of sending controling instruction;
Preferably, described gene test device comprises: electrochemical gene sensor, junctor, the second connection management device is used for providing alternating voltammetry to excite, simulating signal is adjusted, simulating signal is to the conversion of numerary signal, the electron device that carries out data corresponding with processor plate, with the chip heating unit, molecule after described electrochemical gene sensor adopts electrochemical techniques to thing hybridization to be detected carries out mark, realize gene test, described the second connection management device is by the detection signal of alternating voltammetry scanning through mark, and according to the process of the instruction control gene test of described controller, and the temperature of controlling described chip heating unit; Described junctor is used for described electrochemical gene sensor and is connected with the chip heating unit with the second connection management device.
Wherein, described electrochemical gene sensor comprises: detection zone and electric signal transmission end, described detection zone is used for the sample before and after the thing hybridization to be detected is adopted electrochemical techniques, and by the detection signal of alternating voltammetry scanning through mark, be transferred in described the second connection management device via the electric signal transmission end, and then make further analyzing and processing, described detection zone can be DNA electrochemical sensor or electronics electrochemical gene sensor etc., can realize the gene detection tool of electrochemical measuring technique.
Preferably, described upper processing terminal can be common PC, and it comprises:
Be used for creating in treating processes the module that arranges of one or more user's setting options;
Be used for receiving in treating processes the second receiver module of the data signal of controller transmission;
Be used for the computing module processed in the data that treating processes receives the second receiver module according to user's setting option;
For the output module that transmits control signal to controller according to user's setting option in treating processes;
Be used for receiving in treating processes the first receiver module of one or more inputs of user, one or more inputs of described user are corresponding to described one or more user's setting options;
Preferably, described controller comprises: the first management link block, the first processing module and the second processing module;
Described the first management link block is connected with described gene test device, receives the scan-data of the second connection management device output, and carries out data interaction with described the second connection management device, and described the second processing module is carried out interpretation of result to the data of obtaining; Described the first processing module is connected with described the first management link block, is used for described gene test device is carried out control and management, the chip heating unit is controlled, and be used for external communication and connection management.
Preferably, comprise one or more sense terminals, be used for one or more things to be detected are carried out gene test, described sense terminals comprises one or more proofing units, described proofing unit comprises one or more gene test devices, be used for one or more things to be detected are carried out gene test, described chip heating unit comprises one or more chip fire ends, is connected with described one or more gene test devices.
Preferably, described junctor comprises:
The first coupling end is used for connecting described electrochemical gene sensor, is connected with the electrical signal of electrochemical gene sensor;
The environment control end is connected with described chip heating unit, regulates the temperature of control electrochemical gene sensor;
The second electric signal transmission interfaces is connected with described the second connection management device;
Described the first coupling end comprises: electrochemical gene sensor fixed bit and the first electric signal transmission interfaces that is connected with described electrochemical gene sensor, described electrochemical gene sensor fixed bit is used for fixedly electrochemical gene sensor, and described the first electric signal transmission interfaces is used for carrying out data transmission with electrochemical gene sensor;
Described the second electric signal transmission interfaces and described the first electric signal transmission interfaces interconnect, be used for transmission of electric signals between the second connection management device and the described electrochemical gene sensor, to be sent on the second connection management device by the information of electrochemical gene sensor electrical signal transmission, thereby further analyze and process.
The gene test device is connected connection and is connected by junctor with electrochemical gene sensor, wire between the two comprise electrochemical gene sensor power on signal output terminal wire and with the wire of the second connection management device.The switching performance of chip connector parts is very crucial, intends adopting high performance metal clips and the precise injection molding part of special processing to make up assembling, guarantees to reach in contact impedance and work-ing life requirement.
Preferably, described the first processing module comprises: data processing unit (being CPU) and temperature control unit, described data processing unit is used for described gene test device is carried out control and management, and for external communication and connection management, described temperature control unit is used for control chip heating unit, the temperature of regulating in real time electrochemical gene sensor.
Gene test device and controller exist a large amount of data exchange and numerous control lines, connect configuration for reaching high performance hardware connection and being connected, and the first management link block and the two or two connection management device all adopt FPGA as linkage interface among both.FPGA (Field Programmable Gate Array) is field programmable gate array, and it is the product that further develops on the basis of the programming devices such as PAL, GAL, EPLD.It occurs as a kind of semi-custom circuit in application specific integrated circuit (ASIC) field, has both solved the deficiency of custom circuit, has overcome again the limited shortcoming of original programming device gating circuit number.The use of FPGA is very flexible, can produce different circuit functions with a slice FPGA by different programming datas.FPGA provides the highest logic density, the abundantest characteristic and the highest performance.Detection module intends adopting the FPGA device of 10-50 ten thousand gate leves, and the tower switchboard adopts the FPGA device of 1,000,000 gate leves.
The FPGA of gene test device connects the generation of this module of control energizing voltage, and energizing voltage comprises choppy sea and sine-wave superimposed, and waveform is produced by DAC by numerary signal; Chip electrode obtains digital quantity by switch arrays with after amplifying through ADC, and ADC is connected FPGA and is connected control with switch arrays.Be connected with FPGA for sensor provides the hybridization environment to get temperature controlling system, realize temperature control and flow rate control.With the manipulated variable of upper module FPGA and the upper processing terminal by the gene test device, the FPGA of controller connects, and realizes the instruction and data communication.
Controller can connect 8 gene test devices, requirement can be simultaneously to 8 sensors scan, data gathering and analyzing and processing.There is a large amount of instruction and data exchanges between controller and gene test device, for realizing data communication at a high speed, adopts FPGA to carry out two kinds of module hardwares and connect.With the essential scan-data of analyzing each electrode of Time Controller, the data of each electrode must be carried out data transformation and analysis, and the task of number processing is finished by the second processing module DSP fast.DSP obtains at a high speed data by the first management link block (FPGA), and analytical results is equally by FPGA connection data processing unit (CPU) common data storage area, and PC directly obtains analytical results from this zone by the CPU notice.
The transaction management of controller is by the CPU(ARM9 treater) finish, CUP need to carry out control and management to modules and DSP data analysis are initial.CPU is connected the connection of DSP and is carried out hardware by the first management link block (FPGA) and connect with the gene test device, realize that CPU carries out control and management to each control object, and the Status Flag of each control object also feeds back to CPU by FPGA.
The data upload PC of controller realizes by network.The embedded ICP/IP protocol stack of CPU software is connected with the network interface card controller on the hardware.System comprises 3 detection towers, and each detection tower is connected with the route of inside, and route connects with the industry PC of being connected again, thereby realizes detecting the network connection of tower and PC.Local area network by inside and ICP/IP protocol realize detecting the data exchange of tower and embedded industry PC.
Preferably, stating temperature control unit comprises: one or more temperature control components and one or more Temperature Feedback element, described one or more temperature control component corresponds respectively to one or more gene test devices, described one or more temperature feedback element corresponds respectively to one or more gene test devices, described one or more detector unit is followed according to the monitoring temperature of described one or more Temperature Feedback unit to the gene test device, respectively the chip heating unit on one or more gene test devices is controlled in real time.
Preferably, described one or more temperature control component comprises:
Comparing element is used for after the detected temperature simulation digital conversion in one or more Temperature Feedback unit, numerary signal is converted to temperature and target temperature compares;
Controlling elements, result based on the comparison according to parameter prediction temperature variations such as the amplitude of temperature variation, speed, adjusts temperature control work output in real time;
Power element according to the output manipulated variable of controlling elements, is controlled the temperature of described chip heating unit.
Preferably, the second connection management device comprises: digital circuit, modular converter, low-voltage driving module and voltage acquisition reaction circuit;
The work output of the energizing voltage that described Digital Circuit Control low-voltage driving module produces, the voltage acquisition reaction circuit gathers energizing voltage, and modular converter becomes numerary signal with analog signal conversion, the controller collection comprise voltage acquisition and through the numerary signal that analog signal conversion becomes, detect, control and interpretation of result.
The technical solution of the utility model can independently be carried out at most 24 simultaneously and be adopted the electrochemical gene sensor of electrochemical techniques to carry out hybridization check, each sensor requires with arriving with inspection, therefore instrument must possess 24 independent detection modules, each module can both be carried out independent temperature control, satisfying the temperature requirement of different hybridization check stage and different test items, and the essential accurate stable of the temperature of each module control.In order to reach this requirement, each module adopts independent closed loop thermal control unit, system comprises based on the comparing element of ARM9 treater, based on the controlling elements of PWM output control, based on the power element of heating power pipe, amplifies and the feedback element of ADC based on the heating target (being the chip heating unit) of high thermal conductivity copper base with based on high-precision sensing, as shown in Figure 5;
Test item based on each element of ARM9 Treatment Analysis, and obtain the target temperature of current modules by this project chip agreement and crossing phase, obtain the copper base Current Temperatures by Temperature Feedback element (comprising temperature sensors of high precision, high-precision amplification, accurate ADC) simultaneously, after relatively calculating, obtain temperature deviation and manipulated variable, the power element power ratio control outputs to heating tube, copper base is carried out temperature control, make it obtain the temperature of accurate stable.Copper base fully contacts with the chip that inserts detection module, and temperature is transmitted on the chip, for chip hybridization provides suitable temperature environment.
Temperature control algorithm adopts ratio, integration, the differential control based on ARM9, is called for short PID control, claims again PID to regulate.The PID controller algorithm has advantages of good stability, reliable operation, easy to adjust, is one of major technique of Industry Control, is used for hybridization temperature control expection of the present utility model and can reaches good effect.The structure of controlled plant copper base, parameter and random disturbance can not be on top of, can not get accurate mathematical model, other algorithm of control theory is difficult to adopt, therefore the temperature controlling system structure and parameter must rely on the debugging of experience and development to determine, the thereof using PID control techniques is the most convenient, the PID controller is exactly the error according to system, and proportion of utilization, integration, difference gauge are calculated manipulated variable and controlled.Ratio (P) control is the simplest a kind of control mode, the proportional relation of the output of its controller and input error signal, and system exports and has steady-state error when proportional control only.The output of integration (I) control and input error signal be integrated into proportional relation, temperature controlling system to this instrument, if after entering stable state, there is the steady temperature error, this temperature controlling system has the steady temperature error, in order to eliminate steady-state error, in controller, introduce " integral ", integral depends on the integration of time to error, increase along with the time, integral can increase, even if error is very little like this, integral also can strengthen along with the increase of time, the output increase that it promotes controller further reduces the steady temperature error, until equal zero.Therefore, adoption rate+integration (PI) controller algorithm, can make system after entering stable state without the steady temperature error.The output of differential (D) control and the differential (being the velocity of variation of error) of input error signal are proportional, temperature controlling system vibration even unstability can occur in overcoming the regulate process of error, its reason is owing to having larger inertia assembly or lag module (lagging behind may appear in copper base heat conduction) is arranged, have the effect that suppresses error, it changes the variation that always lags behind temperature error.For addressing this problem, make the variation " in advance " of the effect that suppresses error, so that when temperature error approached zero, that suppresses error act as zero, what need to increase is " differential term ", the trend that its can predicated error variation.Like this, have stability controller and the algorithm of PID, the control action kou that suppresses temperature error is equalled zero, even be negative value, thereby avoid the serious overshoot of controlled volume.
Claims (10)
1. an electrochemical gene detection system is characterized in that, comprising: upper processing terminal and sense terminals;
Described sense terminals comprises: gene test device and controller, described gene test device is used for according to hybridization, adopt electrochemical techniques that molecule is carried out mark, and by the detection signal of alternating voltammetry scanning through mark, described controller is controlled described gene test device and is carried out gene test, receives the scan-data of gene test device, and carries out interpretation of result, and analytical results is sent to upper processing terminal, described upper processing terminal is by the real-time adjustment control of sending controling instruction.
2. electrochemical gene detection system according to claim 1, it is characterized in that, described gene test device comprises: electrochemical gene sensor, junctor, the second connection management device and chip heating unit, molecule after described electrochemical gene sensor adopts electrochemical techniques to thing hybridization to be detected carries out mark, realize gene test, described the second connection management device is by the detection signal of alternating voltammetry scanning through mark, and according to the process of the instruction control gene test of described controller, and the output temperature of controlling described chip heating unit; Described junctor is used for described electrochemical gene sensor and is connected with the chip heating unit with the second connection management device respectively.
3. electrochemical gene detection system according to claim 2 is characterized in that, described upper processing terminal comprises:
Be used for receiving in treating processes the second receiver module of the data signal of controller transmission;
Be used for the computing module processed in the data that treating processes receives the second receiver module according to user's setting option;
For the output module that transmits control signal to controller according to user's setting option in treating processes;
Be used for receiving in treating processes the first receiver module of one or more inputs of user, one or more inputs of described user are corresponding to described one or more user's setting options.
4. electrochemical gene detection system according to claim 2 is characterized in that, described controller comprises: the first management link block, the first processing module and the second processing module;
Described the first management link block is connected with described gene test device, receives the scan-data of the second connection management device output, and carries out data interaction with described the second connection management device, and described the second processing module is carried out interpretation of result to the data of obtaining; Described the first processing module is connected with described the first management link block, is used for described gene test device is carried out control and management, the chip heating unit is controlled, and be used for external communication and connection management.
5. electrochemical gene detection system according to claim 4, it is characterized in that, comprise one or more sense terminals, be used for one or more things to be detected are carried out gene test, described sense terminals comprises one or more proofing units, and described proofing unit comprises one or more gene test devices, is used for one or more things to be detected are carried out gene test, described chip heating unit comprises one or more chip fire ends, is connected with described one or more gene test devices.
6. electrochemical gene detection system according to claim 2 is characterized in that, described junctor comprises:
The first coupling end that is connected with described electrochemical gene sensor;
Be connected with described chip heating unit, regulate the environment control end of the temperature of control electrochemical gene sensor;
The second electric signal transmission interfaces that is connected with described the second connection management device;
Described the first coupling end comprises: electrochemical gene sensor fixed bit and the first electric signal transmission interfaces that is connected with described electrochemical gene sensor, described electrochemical gene sensor fixed bit is used for fixedly electrochemical gene sensor, and described the first electric signal transmission interfaces is used for carrying out data transmission with electrochemical gene sensor;
Described the second electric signal transmission interfaces and described the first electric signal transmission interfaces interconnect, and are used for transmission of electric signals between the second connection management device and the described electrochemical gene sensor.
7. electrochemical gene detection system according to claim 4, it is characterized in that, described the first processing module comprises: data processing unit and temperature control unit, described data processing unit is used for described gene test device is carried out control and management, and for external communication and connection management, described temperature control unit is used for control chip heating unit, the temperature of regulating in real time electrochemical gene sensor.
8. electrochemical gene detection system according to claim 7, it is characterized in that, stating temperature control unit comprises: one or more temperature control components and one or more Temperature Feedback element, described one or more temperature control component corresponds respectively to one or more gene test devices, described one or more temperature feedback element corresponds respectively to one or more gene test devices, described one or more detector unit is followed according to the monitoring temperature of described one or more Temperature Feedback unit to the gene test device, respectively the chip heating unit on one or more gene test devices is controlled in real time.
9. electrochemical gene detection system according to claim 8 is characterized in that, described one or more temperature control components comprise:
Comparing element is used for after the detected temperature simulation digital conversion in one or more Temperature Feedback unit, numerary signal is converted to temperature and target temperature compares;
Controlling elements, result based on the comparison according to parameter prediction temperature variations such as the amplitude of temperature variation, speed, adjusts temperature control work output in real time;
Power element according to the output manipulated variable of controlling elements, is controlled the temperature of described chip heating unit.
10. described electrochemical gene detection system is characterized in that according to claim 1～9, and the second connection management device comprises: digital circuit, modular converter, low-voltage driving module and voltage acquisition reaction circuit;
The work output of the energizing voltage that described Digital Circuit Control low-voltage driving module produces, the voltage acquisition reaction circuit gathers energizing voltage, and modular converter becomes numerary signal with analog signal conversion, the controller collection comprise voltage acquisition and through the numerary signal that analog signal conversion becomes, detect, control and interpretation of result.
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| CN 201120569195 CN202705367U (en) | 2011-12-31 | 2011-12-31 | Electrochemical gene detecting system |
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| CN 201120569195 CN202705367U (en) | 2011-12-31 | 2011-12-31 | Electrochemical gene detecting system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554920A (en) * | 2016-12-11 | 2017-04-05 | 天津福德信泰生物科技有限公司 | Electrochemical gene detection system |
| CN108441420A (en) * | 2018-05-11 | 2018-08-24 | 南京思达捷信息科技有限公司 | A kind of gene detection system under controller and its method |
-
2011
- 2011-12-31 CN CN 201120569195 patent/CN202705367U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554920A (en) * | 2016-12-11 | 2017-04-05 | 天津福德信泰生物科技有限公司 | Electrochemical gene detection system |
| CN108441420A (en) * | 2018-05-11 | 2018-08-24 | 南京思达捷信息科技有限公司 | A kind of gene detection system under controller and its method |
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