CN202631544U - External detection reagent box for quick semi-quantitative detection of ferritin concentration - Google Patents
External detection reagent box for quick semi-quantitative detection of ferritin concentration Download PDFInfo
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- CN202631544U CN202631544U CN 201220141022 CN201220141022U CN202631544U CN 202631544 U CN202631544 U CN 202631544U CN 201220141022 CN201220141022 CN 201220141022 CN 201220141022 U CN201220141022 U CN 201220141022U CN 202631544 U CN202631544 U CN 202631544U
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- ferritin
- line
- pad
- quantitative
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Abstract
The utility model discloses an external detection reagent box for a quick semi-quantitative detection of ferritin concentration. The external detection reagent box comprises a reagent box consisting of a ferritin external detection unit and a colorimetric card. The utility model has benefits of semi-quantitative detection, and high detection speed and precision.
Description
Technical field
The utility model relates to the immunology applied technical field, particularly a kind of vitro detection kit that utilizes the colloidal gold immunity chromatography rapid semi-quantitative to detect ferritin concentration.
Background technology
Ferritin (Ferritin) is present in the health in the various cells, and is especially the highest with liver and reticuloendothelial system cell concentration; Its major function is that free state irony too much in the cell is discharged, and uses for health.Therefore, ferritin is main a kind of albumen of storing irony in the bodily tissue, is to keep indispensable a kind of important albumen in the irony balance.Under the normal situation, the ferritin of every 1ng/ml in the serum is equivalent to the ferritin storage capacity of health 8-10mg approximately; So ferritin concentration in the serum; The health status that can directly reflect the human body iron metabolism; Popularize gradually through ferritin concentration determination human body ferro element metabolism status; And find it except as the total stocks in the estimated body, ferritin concentration still can be used as auxiliary characteristics and is used for hepatopathy, and the diagnosis of some malignant tumour.
Clinical practice:
1. ferro element reserves in the direct antimer of ferritin value are as the diagnosis index of anaemia.
2. as some disease auxiliary characteristics of hepatopathy (like liver cancer) particularly, improve the accuracy rate of cancer diagnosis.
Ferritin has reference value to be: the male sex: 15～200ng/ml, women: 12～150ng/ml, children: 7～140ng/ml.Because the difference and the regional disparity of detection method, reference value slightly changes.Generally being lower than 30ng/ml then has the tendency of hypoferric anemia, needs corresponding iron to replenish.Whether there is other disease greater than the 300ng/ml considered.
The immunochromatography colloidal gold technique is novel and comparatively simple diagnostic techniques; Obtained using quite widely, ultimate principle is: utilize a kind of antigen of colloid gold label or antibody, on the NC of reagent film, encapsulate corresponding pairing antigen or antibody; During detection when containing corresponding specific antibody or antigen in the sample; The part formation compound that combines in colloid gold label particle and the sample, chromatography on the NC film then, coated again antigen or antibody capture; Form macroscopic detection (T) line, have or not the judgement of realization the result through detection line.Have easy and simple to handle, reaction fast, high, the high specificity of susceptibility, be fit to on-the-spot the detection and advantage such as economical and practical.
Summary of the invention
The purpose of the utility model is in order to address the above problem, to have designed the vitro detection kit that a kind of rapid semi-quantitative detects ferritin concentration.
The technical scheme that realizes above-mentioned purpose the utility model is that a kind of rapid semi-quantitative detects the vitro detection kit of ferritin concentration, comprises kit, and includes outer detecting unit of ferritin and colorimetric card in the kit.
The outer detecting unit of said ferritin can be the test strips or the reagent card that can detect ferritin.
The structure of said reagent card is that the middle on the PVC plate is pasted nitrocellulose filter; The one end overlap joint of collaurum pad is on the left side of nitrocellulose filter; Overlap the appearance pad on the other end; Overlap joint absorption of sample pad on the right side of nitrocellulose filter, and nitrocellulose filter is provided with detection line T line that encapsulates with ferritin antibody and the nature controlling line C line that encapsulates with anti-rat immune globulin.
Said colorimetric card is provided with chromogenic line colour code and reagent display window.
The said appearance pad of going up is made with nonwoven fabrics, and the print absorption pad is made with absorbent filter.
The rapid semi-quantitative that utilizes the technical scheme of the utility model to make detects the vitro detection kit of ferritin concentration; Utilize colloidal gold immunochromatographimethod technology to realize the outer detectable of sxemiquantitative monitoring siderosome, have easy and simple to handle, equipment and environment relies on less, reaction fast, the sxemiquantitative accuracy is high, specificity is good, be fit to field quick detection and advantage such as economical and practical.
Description of drawings
Fig. 1 is the structural representation that the said rapid semi-quantitative of the utility model detects the vitro detection kit of ferritin concentration;
Fig. 2 is the structural representation of the said reagent card of the utility model;
Among the figure, 1, go up the appearance pad; 2, collaurum pad; 3, nitrocellulose filter; 4, absorption of sample pad; 5, detect the T line; 6, Quality Control C line; 7, PVC plate; 8, kit; 9, detecting unit; 10, colorimetric card; 11, chromogenic line colour code; 12, reagent display window.
Embodiment
Below in conjunction with accompanying drawing the utility model is specifically described; Like Fig. 1 is the structural representation that the said rapid semi-quantitative of the utility model detects the vitro detection kit of ferritin concentration; Fig. 2 is the structural representation of the said reagent card of the utility model, and is as shown in the figure, and a kind of rapid semi-quantitative detects the vitro detection kit of ferritin concentration; Comprise kit 8, and include outer detecting unit 9 of ferritin and colorimetric card 10 in the kit.Wherein, the outer detecting unit of said ferritin can be the test strips or the reagent card that can detect ferritin; The structure of said reagent card is that the middle on the PVC plate 7 is pasted nitrocellulose filter 3; The one end overlap joint of collaurum pad 2 is on the left side of nitrocellulose filter; Overlap appearance pad 1 on the other end; Overlap joint absorption of sample pad 4 on the right side of nitrocellulose filter, and nitrocellulose filter is provided with detection line T line 5 that encapsulates with ferritin antibody and the nature controlling line C line 6 that encapsulates with anti-rat immune globulin; Said colorimetric card is provided with chromogenic line colour code 11 and reagent display window 12; The said appearance pad of going up is made with nonwoven fabrics, and the print absorption pad is made with absorbent filter.
Be example below with the reagent card, concrete detection method be with seized serum, blood plasma or whole blood balance to the greenhouse, reagent card is kept flat; On the collaurum pad, add 10 μ l serum or 20 μ l whole bloods; And on last appearance pad, add 1 (50-80 μ l) sample dilution, sample dissolution collaurum and chromatography, Direct observation with the naked eye then on the NC film; The response situation of C, T line in 15-20 minute; Supporting colorimetric card is placed on the reagent card, and the reagent display window on the colorimetric card is facing on the color change interval on the reagent card, i.e. detection line T line region; The color and the chromogenic line colour code that detect on the T line are compared, judge the ferritin content on the reagent to be detected.
If detecting unit is a test strips, then can directly color change interval on the test strips and the chromogenic line colour code on the colorimetric card be compared, obtain a result.
The ferritin antibody method for coating of said T line and C line is: with 0.003M; PH=7.2 phosphate buffer (PB) to 0.18mg/mL, confirms that at the NC film position carries out the T line with 0.09 μ l/mm parameter and encapsulate with Membrane jetter with antibody dilution, and another confirms that the position encapsulates the C line with ferritin antibody on the NC film simultaneously; After; As for drying room, its environment is with the NC film: temperature 20-25 ℃, humidity is less than 30%.Dry 8-10 hour.
The preparation method of collaurum pad is: prepare the colloid gold particle colloidal sol that diameter is 30-50nm with gold chloride-trisodium citrate reduction method, preparation is got 100ml collaurum liquid in beaker after accomplishing, and uses 0.2MK
2CO
3Transfer to pH7.5, press the mixed that the 100ml colloidal gold solution adds the 0.5mg ferritin antibody, stirring at room 2 hours; Add 1% volume 20% (m/V) bovine serum albumin(BSA) (BSA); 0.5% volume 20% (m/V) polyglycol (PEG), 20000 sealing 20min, centrifugal 30 minutes of 12000rpm abandons supernatant; Redissolve to 76.5ml with the collaurum working fluid, press 1ml solution shop 22cm
2Ratio be layered on equably on the nonwoven fabrics, place drying room again, its environment is: temperature 20-25 ℃, humidity is less than 30%.Dry 2-4 hour, process the collaurum pad.
Technique scheme has only embodied the optimal technical scheme of the utility model technical scheme; The technician in present technique field to some part wherein some changes that possibly make all embodied the principle of the utility model, belong within the protection domain of the utility model.
Claims (5)
1. the vitro detection kit of a rapid semi-quantitative detection ferritin concentration comprises kit (8), it is characterized in that, includes outer detecting unit (9) of ferritin and colorimetric card (10) in the kit.
2. rapid semi-quantitative according to claim 1 detects the vitro detection kit of ferritin concentration, it is characterized in that, the outer detecting unit of said ferritin can be the test strips or the reagent card that can detect ferritin.
3. rapid semi-quantitative according to claim 1 detects the vitro detection kit of ferritin concentration; It is characterized in that; The structure of said reagent card is that the middle on the PVC plate (7) is pasted nitrocellulose filter (3); The one end overlap joint of collaurum pad (2) is on the left side of nitrocellulose filter; Overlap appearance pad (1) on the other end, overlap joint absorption of sample pad (4) on the right side of nitrocellulose filter, and nitrocellulose filter is provided with detection line T line (5) that encapsulates with ferritin antibody and the nature controlling line C line (6) that encapsulates with anti-rat immune globulin.
4. rapid semi-quantitative according to claim 1 detects the vitro detection kit of ferritin concentration, it is characterized in that said colorimetric card is provided with chromogenic line colour code (11) and reagent display window (12).
5. rapid semi-quantitative according to claim 1 detects the vitro detection kit of ferritin concentration, it is characterized in that, the said appearance pad of going up is made with nonwoven fabrics, and the print absorption pad is made with absorbent filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220141022 CN202631544U (en) | 2012-04-06 | 2012-04-06 | External detection reagent box for quick semi-quantitative detection of ferritin concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220141022 CN202631544U (en) | 2012-04-06 | 2012-04-06 | External detection reagent box for quick semi-quantitative detection of ferritin concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202631544U true CN202631544U (en) | 2012-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220141022 Expired - Lifetime CN202631544U (en) | 2012-04-06 | 2012-04-06 | External detection reagent box for quick semi-quantitative detection of ferritin concentration |
Country Status (1)
| Country | Link |
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| CN (1) | CN202631544U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954507A (en) * | 2016-04-26 | 2016-09-21 | 北京言必信科技有限公司 | ELISA kit |
| CN106771272A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method of Furacilin metabolite and detection card in a kind of animal tissue |
| CN107436359A (en) * | 2017-07-25 | 2017-12-05 | 江斌 | Ferritin diagnostic kit |
| US11618032B2 (en) | 2020-08-31 | 2023-04-04 | International Business Machines Corporation | Multiplexed testing strip device |
-
2012
- 2012-04-06 CN CN 201220141022 patent/CN202631544U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954507A (en) * | 2016-04-26 | 2016-09-21 | 北京言必信科技有限公司 | ELISA kit |
| CN106771272A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method of Furacilin metabolite and detection card in a kind of animal tissue |
| CN107436359A (en) * | 2017-07-25 | 2017-12-05 | 江斌 | Ferritin diagnostic kit |
| US11618032B2 (en) | 2020-08-31 | 2023-04-04 | International Business Machines Corporation | Multiplexed testing strip device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: External detection reagent box for quick semi-quantitative detection of ferritin concentration Effective date of registration: 20150204 Granted publication date: 20121226 Pledgee: Tianjin TEDA Small Enterprise Credit Guarantee Center Pledgor: Newscen Coast Bio-Pharmaceutical Co., Ltd. Registration number: 2015120000009 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20160307 Granted publication date: 20121226 Pledgee: Tianjin TEDA Small Enterprise Credit Guarantee Center Pledgor: Newscen Coast Bio-Pharmaceutical Co., Ltd. Registration number: 2015120000009 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 300457 Tianjin Development Zone, Sixth Street, No. 65 Patentee after: Tianjin new torch bio pharmaceutical Limited by Share Ltd Address before: 300457 Tianjin Development Zone, Sixth Street, No. 65 Patentee before: Newscen Coast Bio-Pharmaceutical Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121226 |