CN202626197U - Special enzymolysis tank for magnetic immobilized enzyme - Google Patents
Special enzymolysis tank for magnetic immobilized enzyme Download PDFInfo
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- CN202626197U CN202626197U CN 201220341841 CN201220341841U CN202626197U CN 202626197 U CN202626197 U CN 202626197U CN 201220341841 CN201220341841 CN 201220341841 CN 201220341841 U CN201220341841 U CN 201220341841U CN 202626197 U CN202626197 U CN 202626197U
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- tank body
- immobilized enzyme
- magnetic immobilized
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
A special enzymolysis tank for magnetic immobilized enzyme includes a tank body with a thermal insulation layer and a corresponding power supply, wherein a feeding opening, a sampling hole and a manhole are formed at the top of the tank body, a discharging opening is arranged at the center of the bottom part of the tank body, and a stirring mechanism is arranged inside the tank body. The special enzymolysis tank is characterized in that an electromagnetic device is arranged at the bottom part inside the tank body and includes an internal coil encapsulated by resin; an electromagnetic sucker is arranged on the upper surface of the electromagnetic device; the electromagnetic device is provided with an annular structure encircling the discharging opening, and the bottom matched with the bottom part of the tank body and closely attached to the bottom part of the tank body; and the intensity of the magnetism can be controlled by current. The special enzymolysis tank can instantly recover and release the magnetic immobilized enzyme, recovers completely and is easy to clean after the reaction, discharges the waste enzyme in a centralized way, is energy-saving and environment-friendly, facilitates the scientific and reasonable utilization of the magnetic immobilized enzyme, facilitates the industrial popularization and application, can completely recover the magnetic immobilized enzyme, and realize the waste enzyme centralized treatment and discharge when the magnetic immobilized enzyme cannot be applied to the industrial production, and prevents and controls the environmental pollution.
Description
Technical field
The utility model relates to a kind of enzymolysis device, is specifically related to a kind of magnetic immobilized enzyme enzymatic vessel, is applied to the enzyme digestion reaction of various magnetic immobilized enzyme.
Background technology
The magnetic immobilized enzyme is with the catalytic activity of resolvase fully or be substantially limited in the process in the certain space scope; Ultimate principle is: magnetic microsphere is directly put into the mixing solutions that contains a certain amount of enzyme; Make enzyme and microsphere surface reactive group full cross-linked; Immobilized reactant utilizes the external magnetic field that it is separated, and then reuses after finishing.
The magnetic immobilized enzyme is when keeping the efficient single-minded and gentle enzymic catalytic reaction characteristic of resolvase; Overcome the weak point of resolvase again; Demonstrate following advantage: the one, the production aspect: magnetic immobilized enzyme package stability is high, Separation and Recovery is easy, operation is controlled; Can realize continuous automatic production, improve the quality of products; The 2nd, economic aspect: the magnetic immobilized enzyme can be repeatedly used, and can reduce starting material and energy consumption; The 3rd, the environmental protection aspect: the magnetic immobilized enzyme can reduce the ecotope that the industrial residue discharging can reduce or prevent and remedy pollution, and meets the STRATEGIES OF SUSTAINABLE DEVELOPMENT requirement.
Along with developing rapidly of society, the magnetic enzyme immobilization technology obtains developing rapidly and uses widely in the research Showed Very Brisk in fields such as chemical industry, food and environmental science: but like the reaction of magnetic immobilized enzyme catalysis number of chemical, can improve repeating utilization factor; The magnetic immobilized enzyme has stability preferably, has good application prospect aspect food, fermentation industry, glucose industry and the dairy products industry; With environmental pollution be on the rise and administer urgent day by day, the magnetic immobilized enzyme also will have important use at environmental science, so the magnetic immobilized enzyme is the inexorable trend of immobilization technology development.
Tradition enzymolysis device mainly is made up of tank body and the support and the stirring mechanism that support tank body; Nonmagnetic device; Therefore reclaim with traditional enzymolysis apparatus processes machine that magnetic immobilized enzyme operation is loaded down with trivial details, low in economic efficiency, recovery is not thorough; Lose the advantage of magnetic immobilized enzyme safety, cleaning, convenient, fast recycling, caused the increase of enzymolysis cost.
Summary of the invention
In order to solve the deficiency that traditional enzymatic vessel reclaims the magnetic immobilized enzyme; The utility model provides a kind of magnetic immobilized enzyme special-purpose enzymatic vessel; This enzymatic vessel can not only be realized moment recovery, the abrupt release to the magnetic immobilized enzyme; Can also guarantee thoroughness and reusable convenience that the magnetic immobilization reclaims, for the scientific utilization of magnetic immobilized enzyme provides condition.
For achieving the above object, the technical scheme that the utility model adopts is following:
The special-purpose enzymatic vessel of a kind of magnetic immobilized enzyme; Comprise that outside surface is provided with the tank body and the corresponding power supply of thermal insulation layer, the top of this tank body is provided with drain hole, thief hole, manhole, and bottom central is provided with discharge port; Inside is provided with stirring mechanism, it is characterized in that this tank interior bottom surface is provided with calutron.
Described calutron comprises the Inside coil by the resin embedding, and the calutron upper surface is a magneticchuck.
Described calutron is the ring texture body around discharge port, and bottom shape and tank interior bottom shape match, and closely is fitted in the bottom of tank body.
Described calutron is by the power of power supply control adjustment magnetic, and promptly the power of calutron magnetic can change, and magnetic can disappear because of the disappearance of electric current.
Described stirring mechanism comprises vertically intravital at jar, as to have a many group paddle wheels stir shaft, and this stir shaft is driven by the agitator motor that is arranged on the tank body top.
The utility model is simple in structure, easy to operate, and has the following advantages:
1, the moment that can realize the magnetic immobilized enzyme reclaims, abrupt release, and calutron be annular, is convenient to the discharging of feed liquid, and the outside is set as smooth circular-arc, can prevent effectively that the dead angle from producing, and is prone to cleaning.
2, can thoroughly reclaim the magnetic immobilized enzyme, the product quality problem that causes when avoiding reclaiming thoroughly.
3, can realize when magnetic immobilized enzyme enzyme live reduction can not be applied to industrial production the time useless enzyme focus on discharging, control environmental pollution.
Description of drawings
Fig. 1 is the overall structure synoptic diagram of the utility model.
Fig. 2 is the vertical view of the calutron of the utility model.
Fig. 3 is the side-view of the calutron of the utility model.
Fig. 4 is the stereographic map of the calutron of the utility model.
Wherein: 1, tank body, 11, drain hole, 12, thief hole, 13, manhole, 14, discharge port, 2, thermal insulation layer, 3, stirring mechanism, 31, agitator motor, 32, stir shaft, 33, paddle wheel, 4, calutron, 41, Inside coil, 42, magneticchuck.
Embodiment
Below in conjunction with accompanying drawing the utility model embodiment is further specified:
As shown in Figure 1; The special-purpose enzymatic vessel of a kind of magnetic immobilized enzyme; Comprise that outside surface is provided with the tank body 1 and the corresponding power supply of thermal insulation layer 2, the top of this tank body 1 is provided with drain hole 11, thief hole 12, manhole 13, and bottom central is provided with discharge port 14; Inside is provided with stirring mechanism 3, it is characterized in that this tank body 1 inner bottom part is provided with calutron 4.
As shown in Figure 1, described stirring mechanism 3 comprises a stir shaft 32 vertically in tank body 1, that have many group paddle wheels 33, and this stir shaft 32 is by 31 drivings of the agitator motor that is arranged on tank body 1 top.Wherein, stir shaft 33 upper ends be connected with agitator motor 31, the lower end can rotational fixation on the bottom surface of tank body 1.
Like Fig. 1, shown in 2, described calutron 4 comprises the Inside coil 41 by the resin embedding, and calutron 4 upper surfaces are the magneticchuck 42 that is used to adsorb the magnetic immobilized enzyme; Wherein magneticchuck 42 can produce magnetic when Inside coil 41 energisings, can adopt existing commercially available prod.
Described calutron 4 is the ring texture bodies around discharge port 14; Obviously owing to adopt the mode of resin to Inside coil 41 embeddings; Resin itself has very high plasticity-; Therefore the bottom shape of calutron 4 is processed into the structure that matches with tank body 1 inner bottom surface shape and accomplishes easily, thereby can closely fit together, be unlikely to produce the dead angle with tank body 1 inner bottom surface.Shown in Fig. 1～4, the bottom design of calutron 4 becomes to have the shape of smooth circular arc chamfering, and is consistent with the inner bottom surface of tank body 1, thereby can closely be shelved on the bottom of tank body 1.
Described calutron 4 is by the power of power supply control adjustment magnetic, and promptly the power of calutron 4 magnetic can change, and magnetic can disappear because of the disappearance of electric current.
During use, reactant and an amount of magnetic immobilized enzyme are put into enzymatic vessel, start agitator motor 31; Make whipped-up structure 3 runnings; Enzymolysis to required state stops to stir, and opens the power supply of control calutron 4; Moment is adsorbed on the magnetic immobilized enzyme on the magneticchuck 42, and zymolyte is discharged from discharge port 14; Again blowing breaks off the power supply of controlling calutron 4, starts agitator motor 31; Make whipped-up structure 3 running, enzymolysis once more, above process repeats repeatedly; Till the magnetic immobilized enzyme does not reach enzyme requirement alive, be with zymolyte at last after discharge port 14 discharges, break off the power supply of control calutron 4; Discharge depleted magnetic immobilized enzyme with flushing with clean water from discharge port 14, focus on discharging.Because it is smooth circular-arc that the outside of calutron 4 is set as, can prevent effectively that the dead angle from producing, and cleans more thorough.
Claims (4)
1. the special-purpose enzymatic vessel of a magnetic immobilized enzyme; Comprise that outside surface is provided with tank body of thermal insulation layer (2) (1) and corresponding power supply; The top of this tank body (1) is provided with drain hole (11), thief hole (12), manhole (13); Bottom central is provided with discharge port (14), and inside is provided with stirring mechanism (3), it is characterized in that this tank body (1) inner bottom surface is provided with calutron (4).
2. the special-purpose enzymatic vessel of magnetic immobilized enzyme according to claim 1 is characterized in that described calutron (4) comprises the Inside coil (41) by the resin embedding, and calutron (4) upper surface is magneticchuck (42).
3. the special-purpose enzymatic vessel of magnetic immobilized enzyme according to claim 2; It is characterized in that described calutron (4) is the ring texture body around discharge port (14); And bottom shape and tank body (1) inner bottom surface shape matches, and closely the be fitted in tank body bottom of (1).
4. the special-purpose enzymatic vessel of magnetic immobilized enzyme according to claim 1 is characterized in that the power of described calutron (4) by power supply control adjustment magnetic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220341841 CN202626197U (en) | 2012-07-13 | 2012-07-13 | Special enzymolysis tank for magnetic immobilized enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220341841 CN202626197U (en) | 2012-07-13 | 2012-07-13 | Special enzymolysis tank for magnetic immobilized enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202626197U true CN202626197U (en) | 2012-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220341841 Expired - Fee Related CN202626197U (en) | 2012-07-13 | 2012-07-13 | Special enzymolysis tank for magnetic immobilized enzyme |
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| CN (1) | CN202626197U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087891A (en) * | 2013-01-21 | 2013-05-08 | 浙江农林大学 | Device and method for reducing and removing urea in fermented wine |
| CN105861307A (en) * | 2016-05-13 | 2016-08-17 | 山东师范大学 | Production device of magnetically-recyclable biological enzymes suitable for industrialized application |
| CN108220159A (en) * | 2018-04-08 | 2018-06-29 | 北京电子科技职业学院 | It is a kind of for the bioconversion reactor of immobilized lactase and the preparation method of low lactose milk |
-
2012
- 2012-07-13 CN CN 201220341841 patent/CN202626197U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087891A (en) * | 2013-01-21 | 2013-05-08 | 浙江农林大学 | Device and method for reducing and removing urea in fermented wine |
| CN103087891B (en) * | 2013-01-21 | 2014-07-16 | 浙江农林大学 | Device and method for reducing and removing urea in fermented wine |
| CN105861307A (en) * | 2016-05-13 | 2016-08-17 | 山东师范大学 | Production device of magnetically-recyclable biological enzymes suitable for industrialized application |
| CN105861307B (en) * | 2016-05-13 | 2018-08-28 | 山东师范大学 | Suitable for industrial applications can magnetic recovery biological enzyme process units |
| CN108220159A (en) * | 2018-04-08 | 2018-06-29 | 北京电子科技职业学院 | It is a kind of for the bioconversion reactor of immobilized lactase and the preparation method of low lactose milk |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121226 Termination date: 20130713 |